Stem-loop sequence mmu-mir-136

Accession

MI0000162

Symbol

MGI:Mir136

Description

Mus musculus miR-136 stem-loop

Gene family

MIPF0000099; mir-136

Stem-loop

        c     uuu          uuc 
gaggacuc auuug   ugaugaugga   u
|||||||| |||||   ||||||||||   u
cuucugag uaaac   gcuacuaccu   a
        -     ucu          cga 

Deep sequencing

397738 reads, 70 experiments

Comments

The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [4].

Genome context

• Coordinates (GRCm38): chr12: 109595327-109595388 [+]
• Overlapping transcripts:

  sense

  ENSMUST00000093621 ; Mir136-201; exon 1

  antisense

  ENSMUST00000149046 ; Rtl1-201; exon 2

Clustered miRNAs

< 10kb from mmu-mir-136

• mmu-mir-337: chr12: 109585789-109585885 [+]
• mmu-mir-3544: chr12: 109585813-109585873 [-]
• mmu-mir-540: chr12: 109586080-109586146 [+]
• mmu-mir-665: chr12: 109586314-109586407 [+]
• mmu-mir-3070a: chr12: 109587943-109588031 [+]
• mmu-mir-3070b: chr12: 109588592-109588680 [+]
• mmu-mir-431: chr12: 109590447-109590537 [+]
• mmu-mir-433: chr12: 109591715-109591838 [+]
• mmu-mir-127: chr12: 109592846-109592915 [+]
• mmu-mir-434: chr12: 109594506-109594599 [+]
• mmu-mir-432: chr12: 109594956-109595030 [+]
• mmu-mir-3071: chr12: 109595318-109595397 [-]
• mmu-mir-136: chr12: 109595327-109595388 [+]

Database links

• EMBL: AJ459747;MMU459747
• ENTREZGENE: 387154;Mir136
• MGI: 2676821;Mir136

Mature sequence mmu-miR-136-5p

• Accession: MIMAT0000148
• Previous IDs: mmu-miR-136
• Sequence: 5 - acuccauuuguuuugaugaugg - 26
• Deep sequencing: 351955 reads, 56 experiments
• Evidence: experimental; cloned [1-2,4], PCR [3], Solexa [5-6]
• Validated targets:
  • TARBASE:mmu-miR-136-5p
• Predicted targets:
  • DIANA-MICROT:mmu-miR-136-5p
  • MICRORNA.ORG:mmu-miR-136-5p
  • MIRDB:mmu-miR-136-5p
  • RNA22-MMU:mmu-miR-136-5p
  • TARGETSCAN-VERT:mmu-miR-136

Mature sequence mmu-miR-136-3p

• Accession: MIMAT0004532
• Previous IDs: mmu-miR-136*
• Sequence: 40 - aucaucgucucaaaugagucuu - 61
• Deep sequencing: 45694 reads, 55 experiments
• Evidence: experimental; cloned [4], Solexa [5-6]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-136-3p
  • MICRORNA.ORG:mmu-miR-136-3p
  • MIRDB:mmu-miR-136-3p
  • RNA22-MMU:mmu-miR-136-3p

References

1. PMID:12007417 "Identification of tissue-specific microRNAs from mouse" Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T Curr Biol. 12:735-739(2002).
2. PMID:15538371 "A pancreatic islet-specific microRNA regulates insulin secretion" Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P, Stoffel M Nature. 432:226-230(2004).
3. PMID:15854907 "RNAi-mediated allelic trans-interaction at the imprinted Rtl1/Peg11 locus" Davis E, Caiment F, Tordoir X, Cavaille J, Ferguson-Smith A, Cockett N, Georges M, Charlier C Curr Biol. 15:743-749(2005).
4. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
5. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
6. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).