miRBase entry: ath-MIR166a

Stem-loop ath-MIR166a


Accession
MI0000201
Description
Arabidopsis thaliana ath-MIR166a precursor miRNA
Gene family
MIPF0000004; MIR166

Literature search
39 open access papers mention ath-MIR166a
(333 sentences)

Sequence

aggggcuuucucuuuugaggGGACUGUUGUCUGGCUCGAGGacucuggcucgcucuauucauguuggaucucuuucgaucuaacaaucgaauugaaccuucagauuucagauuugauuaggguuuuagcgucuUCGGACCAGGCUUCAUUCCCCccaauuguugcucccu
.(((((.....(..(((.(((((.((..((((((.((((((((.((((...((((((.((((((((((((......)))))))))((((((((((....))))..))).))).))).)))))).)))).)))))))).))))))..)).))))).)))..)..)))))..

Structure
-a     uuucu uu   a     C  UU      C        u    cuc      u   ----------------------------         uc 
  ggggc     c  uug ggGGA UG  GUCUGG UCGAGGac cugg   gcucua uca                            uguuggauc  u
  |||||     |  ||| ||||| ||  |||||| |||||||| ||||   |||||| |||                            |||||||||   
  ccucg     g  aac CCCCU AC  CGGACC GGCUucug gauu   ugggau agu                            acaaucuag  u
uc     ---uu uu   c     U  UU      A        c    --u      u   uuagacuuuagacuuccaaguuaagcua         cu 


Annotation confidence Not enough data
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Comments
MIR166a, like miR165, is thought to target mRNAs coding for HD-Zip transcription factors including Phabulosa (PHB) and Phavoluta (PHV) that regulate axillary meristem initiation and leaf development [2].

Genome context
chr2: 19176108-19176277 [+]

Database links

Mature ath-miR166a-3p

Accession MIMAT0000189
Description Arabidopsis thaliana ath-miR166a-3p mature miRNA
Sequence 134 - UCGGACCAGGCUUCAUUCCCC - 154
Evidence experimental
cloned [1,3], Northern [1], 5'RACE [3], 454 [4-5], MPSS [4], Illumina [6]

Mature ath-miR166a-5p

Accession MIMAT0031880
Description Arabidopsis thaliana ath-miR166a-5p mature miRNA
Sequence 21 - GGACUGUUGUCUGGCUCGAGG - 41
Evidence experimental
Illumina [7]

References

  1. PubMed ID: 12101121
    MicroRNAs in plants
    "Reinhart BJ, Weinstein EG, Rhoades MW, Bartel B, Bartel DP"
    "Genes Dev (2002) 16:1616-1626

  2. PubMed ID: 12202040
    Prediction of plant microRNA targets
    "Rhoades MW, Reinhart BJ, Lim LP, Burge CB, Bartel B, Bartel DP"
    "Cell (2002) 110:513-520

  3. PubMed ID: 16040653
    Expression of Arabidopsis MIRNA genes
    "Xie Z, Allen E, Fahlgren N, Calamar A, Givan SA, Carrington JC"
    "Plant Physiol (2005) 138:2145-2154

  4. PubMed ID: 16954541
    MicroRNAs and other small RNAs enriched in the Arabidopsis RNA-dependent RNA polymerase-2 mutant
    "Lu C, Kulkarni K, Souret FF, MuthuValliappan R, Tej SS, Poethig RS, Henderson IR, Jacobsen SE, Wang W, Green PJ, Meyers BC"
    "Genome Res (2006) 16:1276-1288

  5. PubMed ID: 17182867
    A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana
    "Rajagopalan R, Vaucheret H, Trejo J, Bartel DP"
    "Genes Dev (2006) 20:3407-3425

  6. PubMed ID: 19815687
    Hypoxia-responsive microRNAs and trans-acting small interfering RNAs in Arabidopsis
    "Moldovan D, Spriggs A, Yang J, Pogson BJ, Dennis ES, Wilson IW"
    "J Exp Bot (2010) 61:165-177

  7. PubMed ID: 24119003
    Integrated RNA-seq and sRNA-seq analysis identifies novel nitrate-responsive genes in Arabidopsis thaliana roots
    Vidal EA, Moyano TC, Krouk G, Katari MS, Tanurdzic M, McCombie WR, Coruzzi GM, Gutiérrez RA
    BMC Genomics (2013) 14:701