Stem-loop sequence hsa-mir-192

Accession

MI0000234 (change log)

Symbol

HGNC:MIR192

Description

Homo sapiens miR-192 stem-loop

Gene family

MIPF0000063; mir-192

Literature search

214 open access papers mention hsa-mir-192
(1082 sentences)

Stem-loop

   gccgagacc   u  --   g g  c         -     a       ugcucuc 
5'          gag gc  aca g cu ugaccuaug aauug cagccag       g
            ||| ||  ||| | || ||||||||| ||||| |||||||        
3'          cuc cg  ugu u ga acuggauac uuaac gucgguc       u
   cgaccguaa   -  cu   a g  c         c     c       uccccuc 

Deep sequencing

1045613 reads, 781 reads per million, 159 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

Lagos-Quintana et al. validated the presence of an 18 nt excised sequence by cloning [1]. Lim et al. predicted the miR by computational methods using conservation with mouse and Fugu rubripes sequences. Expression of the excised miR was validated in zebrafish, and the 5' end mapped by PCR [2]. The 3' ends of the reported sequences differ by 3 nt - this entry contains the longer sequence. Lim et al. report three separate copies of this gene named mir-192-1, -2 and -3 based on 2001 human genome assemblies [2]. Subsequent assemblies suggest the presence of only one gene located on chromosome 11.

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chr11: 64891137-64891246 [-]
• Overlapping transcripts:

  sense

  OTTHUMT00000385272 ; SYVN1-012; intron 1

  ENST00000530451 ; SYVN1-012; intron 1

  antisense

  OTTHUMT00000385288 ; MRPL49-002; intron 1

  OTTHUMT00000385289 ; MRPL49-007; intron 1

  OTTHUMT00000385290 ; MRPL49-008; intron 1

  OTTHUMT00000385291 ; MRPL49-004; intron 1

  OTTHUMT00000385292 ; MRPL49-006; intron 1

  OTTHUMT00000385293 ; MRPL49-001; intron 1

  OTTHUMT00000385294 ; MRPL49-009; intron 1

  OTTHUMT00000385295 ; MRPL49-005; intron 1

  ENST00000524482 ; MRPL49-002; intron 1

  ENST00000528529 ; MRPL49-007; intron 1

  ENST00000534078 ; MRPL49-008; intron 1

  ENST00000526319 ; MRPL49-004; intron 1

  ENST00000526171 ; MRPL49-006; intron 1

  ENST00000279242 ; MRPL49-001; intron 1

  ENST00000531705 ; MRPL49-009; intron 1

  ENST00000533943 ; MRPL49-005; intron 1

Clustered miRNAs

< 10kb from hsa-mir-192

• hsa-mir-6750: chr11: 64898363-64898437 [-]
• hsa-mir-194-2: chr11: 64891355-64891439 [-]
• hsa-mir-192: chr11: 64891137-64891246 [-]

Database links

• EntrezGene:MIR192
• HGNC:MIR192
• RNAcentral:URS00006ECF62_9606
• miRBase Tracker:MI0000234

Mature sequence hsa-miR-192-5p

• Accession: MIMAT0000222
• Previous IDs: hsa-miR-192
• Sequence: 24 - cugaccuaugaauugacagcc - 44
• Deep sequencing: 1045071 reads, 159 experiments
• Evidence: experimental; cloned [3-4]
• Database links:
  • RNAcentral:URS0000155642_9606
• Predicted targets:
  • TargetMiner:hsa-miR-192-5p
  • TargetScanVert:hsa-miR-192-5p
  • miRDB:hsa-miR-192-5p
  • microrna.org:hsa-miR-192-5p

Mature sequence hsa-miR-192-3p

• Accession: MIMAT0004543
• Previous IDs: hsa-miR-192*
• Sequence: 67 - cugccaauuccauaggucacag - 88
• Deep sequencing: 452 reads, 93 experiments
• Evidence: experimental; cloned [4]
• Database links:
  • RNAcentral:URS00000B59A2_9606
• Predicted targets:
  • TargetMiner:hsa-miR-192-3p
  • TargetScanVert:hsa-miR-192-3p
  • miRDB:hsa-miR-192-3p
  • microrna.org:hsa-miR-192-3p

References

1. PMID:12554859 "New microRNAs from mouse and human" Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).
2. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
3. PMID:14573789 "Reduced accumulation of specific microRNAs in colorectal neoplasia" Michael MZ, O' Connor SM, van Holst Pellekaan NG, Young GP, James RJ Mol Cancer Res. 1:882-891(2003).
4. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).