Stem-loop sequence mmu-mir-194-1

AccessionMI0000236
Previous IDsmmu-mir-194
Symbol MGI:Mir194-1
DescriptionMus musculus miR-194-1 stem-loop
Gene family MIPF0000055; mir-194
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled mir-194_microRNA_precursor_family. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

In molecular biology, miR-194 microRNA precursor is a small non-coding RNA gene that regulated gene expression. Its expression has been verified in mouse (MI0000236, MI0000733) and in human (MI0000488, MI0000732). mir-194 appears to be a vertebrate-specific miRNA and has now been predicted or experimentally confirmed in a range of vertebrate species (MIPF0000055). The mature microRNA is processed from the longer hairpin precursor by the Dicer enzyme. In this case, the mature sequence is excised from the 5' arm of the hairpin.

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Stem-loop
         u         a      g    --   u 
5' aucggg guaacagca cuccau ugga  cug g
   |||||| ||||||||| |||||| ||||  |||  
3' uagucu cauugucgu gaggug accu  ggc c
         u         c      -    ua   u 
Get sequence
Deep sequencing
331746 reads, 2.4e+03 reads per million, 108 experiments
Confidence Annotation confidence: high
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Comments

Lagos-Quintana cloned miR-194 from mouse kidney tissue [1]. Michael et al. subsequently verified expression of miR-194 in human [2]. Two putative pairs of orthologous hairpin precursors structures are found in mouse (mir-194-1 (MI0000236) on chromosome 1, and mir-194-2 (MI0000733) on chromosome 19) and human (mir-194-1 (MI0000488) on chromosome 1, and mir-194-2 (MI0000732) on chromosome 11).

Genome context
Coordinates (GRCm38) Overlapping transcripts
chr1: 185313319-185313385 [+]
antisense
OTTMUST00000051852 ; Iars2-001; intron 12
OTTMUST00000051855 ; Iars2-004; intron 12
OTTMUST00000051856 ; Iars2-005; intron 12
ENSMUST00000027921 ; Iars2-001; intron 12
ENSMUST00000110975 ; Iars2-005; intron 12
ENSMUST00000110974 ; Iars2-004; intron 12
Clustered miRNAs
< 10kb from mmu-mir-194-1
mmu-mir-194-1chr1: 185313319-185313385 [+]
mmu-mir-215chr1: 185313581-185313692 [+]
Database links

Mature sequence mmu-miR-194-5p

Accession MIMAT0000224
Previous IDsmmu-miR-194
Sequence

7 - 

uguaacagcaacuccaugugga

 - 28

Get sequence
Deep sequencing372357 reads, 91 experiments
Evidence experimental; cloned [1,3], Illumina [4-5]
Validated targets
Predicted targets

Mature sequence mmu-miR-194-1-3p

Accession MIMAT0016999
Previous IDsmmu-miR-194-1*
Sequence

42 - 

ccaguggagcugcuguuacuuc

 - 63

Get sequence
Deep sequencing108 reads, 12 experiments
Evidence experimental; Illumina [5]
Predicted targets

References

1
PMID:12554859 "New microRNAs from mouse and human" Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).
2
PMID:14573789 "Reduced accumulation of specific microRNAs in colorectal neoplasia" Michael MZ, O' Connor SM, van Holst Pellekaan NG, Young GP, James RJ Mol Cancer Res. 1:882-891(2003).
3
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4
PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5
PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).