Stem-loop sequence hsa-mir-204

Accession

MI0000284

Symbol

HGNC:MIR204

Description

Homo sapiens miR-204 stem-loop

Gene family

MIPF0000042; mir-204

Stem-loop

   ggcuacagucuuucu   -  -    ucg     u          a     u    gagaau 
5'                uca ug ugac   uggac ucccuuuguc uccua gccu      a
                  ||| || ||||   ||||| |||||||||| ||||| ||||      u
3'                ggu ac acug   acuug agggaaacgg agggu cgga      a
   --------------c   c  u    uua     c          a     -    ggaagu 

Deep sequencing

4851 reads, 1.59e+03 reads per million, 53 experiments

Confidence

Feedback: Do you believe this miRNA is real?

Comments

This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences [1]. Expression of the excised miR has been validated in zebrafish, and the ends mapped by cloning. Landgraf et al. confirm expression in human [2].

Genome context

• Coordinates (GRCh38): chr9: 70809975-70810084 [-]
• Overlapping transcripts:

  intergenic

Database links

• MIRBASE-TRACKER:MI0000284
• ENTREZGENE: 406987;MIR204
• HGNC: 31582;MIR204
• RNACENTRAL:URS000039AE8A/9606

Mature sequence hsa-miR-204-5p

• Accession: MIMAT0000265
• Previous IDs: hsa-miR-204
• Sequence: 33 - uucccuuugucauccuaugccu - 54
• Deep sequencing: 1445 reads, 40 experiments
• Evidence: experimental; cloned [2]
• Validated targets:
  • MIRTARBASE:hsa-miR-204-5p
  • TARBASE:hsa-miR-204-5p
• Predicted targets:
  • DIANA-MICROT:hsa-miR-204-5p
  • MICRORNA.ORG:hsa-miR-204-5p
  • MIRDB:hsa-miR-204-5p
  • RNA22-HSA:hsa-miR-204-5p
  • TARGETMINER:hsa-miR-204-5p
  • TARGETSCAN-VERT:hsa-miR-204
  • PICTAR-VERT:hsa-miR-204

Mature sequence hsa-miR-204-3p

• Accession: MIMAT0022693
• Sequence: 72 - gcugggaaggcaaagggacgu - 92
• Deep sequencing: 259 reads, 35 experiments
• Evidence: not experimental
• Predicted targets:
  • DIANA-MICROT:hsa-miR-204-3p
  • MIRDB:hsa-miR-204-3p
  • TARGETMINER:hsa-miR-204-3p

References

1. PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).