Stem-loop sequence mmu-mir-300

Accession

MI0000400 (change log)

Symbol

MGI:Mir300

Description

Mus musculus miR-300 stem-loop

Gene family

MIPF0000018; mir-154

Literature search

12 open access papers mention mmu-mir-300
(27 sentences)

Stem-loop

      a           g  au             gc   a 
5' gcu cuugaagagag uu  ccuuuguguguuu  uuu c
   ||| ||||||||||| ||  |||||||||||||  |||  
3' cga gagcuucucuc aa  gggaacguauaag  aag g
      g           g  -c             ua   c 

Deep sequencing

266047 reads, 1.06e+03 reads per million, 90 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr12: 109724313-109724391 [+]
• Overlapping transcripts:

  sense

  ENSMUST00000083485 ; mmu-mir-300.1-201; exon 1

Clustered miRNAs

< 10kb from mmu-mir-300

• mmu-mir-494: chr12: 109715318-109715402 [+]
• mmu-mir-679: chr12: 109715577-109715650 [+]
• mmu-mir-1193: chr12: 109715671-109715791 [+]
• mmu-mir-666: chr12: 109717085-109717183 [+]
• mmu-mir-543: chr12: 109717258-109717333 [+]
• mmu-mir-495: chr12: 109718754-109718816 [+]
• mmu-mir-667: chr12: 109720006-109720097 [+]
• mmu-mir-376c: chr12: 109722718-109722803 [+]
• mmu-mir-654: chr12: 109723218-109723301 [+]
• mmu-mir-376b: chr12: 109723458-109723539 [+]
• mmu-mir-376a: chr12: 109723781-109723848 [+]
• mmu-mir-300: chr12: 109724313-109724391 [+]
• mmu-mir-381: chr12: 109726822-109726896 [+]
• mmu-mir-487b: chr12: 109727333-109727414 [+]
• mmu-mir-539: chr12: 109728129-109728202 [+]
• mmu-mir-544: chr12: 109729325-109729402 [+]
• mmu-mir-382: chr12: 109733771-109733846 [+]
• mmu-mir-134: chr12: 109734139-109734209 [+]

Database links

• EntrezGene:Mirn300
• MGI:Mir300
• RNAcentral:URS000071A79E_10090
• miRBase Tracker:MI0000400

Mature sequence mmu-miR-300-5p

• Accession: MIMAT0004578
• Previous IDs: mmu-miR-300*
• Sequence: 6 - uugaagagagguuauccuuugu - 27
• Deep sequencing: 4573 reads, 51 experiments
• Evidence: experimental; cloned [2], Illumina [3-4]
• Database links:
  • RNAcentral:URS000002CC9A_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-300-5p
  • miRDB:mmu-miR-300-5p
  • microrna.org:mmu-miR-300-5p

Mature sequence mmu-miR-300-3p

• Accession: MIMAT0000378
• Previous IDs: mmu-miR-300
• Sequence: 51 - uaugcaagggcaagcucucuuc - 72
• Deep sequencing: 261469 reads, 89 experiments
• Evidence: experimental; cloned [1-2], Illumina [3-4]
• Database links:
  • RNAcentral:URS000022074F_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-300-3p
  • miRDB:mmu-miR-300-3p
  • microrna.org:mmu-miR-300-3p

References

1. PMID:12919684 "Embryonic stem cell-specific MicroRNAs" Houbaviy HB, Murray MF, Sharp PA Dev Cell. 5:351-358(2003).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
3. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
4. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).