Stem-loop sequence dme-mir-312

Accession MI0000424
Description Drosophila melanogaster miR-312 stem-loop
Gene family MIPF0000573; mir-312
Stem-loop 
    u   u    a    g     u   cauu 
gauu ggu cguc caag gcaau cug    u
|||| ||| |||| |||| ||||| |||    u
uuag ccg gcag guuc cguua gau    u
    u   -    a    a     u   caau
Get sequence
Deep sequencing
45031 reads, 48 experiments
Comments

miR-312 was identified and 5' end mapped by cloning. Reference [1] reports a length distribution of 22-23 nt, with 22 nt most commonly expressed. The sequence is localised to chromosome 2R.

Genome context
Coordinates (BDGP5.0) Overlapping transcripts
2R: 16471561-16471626 [-]
intergenic
Clustered miRNAs
< 10kb from dme-mir-312
dme-mir-992 2R: 16472819-16472910 [-]
dme-mir-991 2R: 16472705-16472800 [-]
dme-mir-2498 2R: 16472214-16472325 [-]
dme-mir-313 2R: 16471695-16471765 [-]
dme-mir-312 2R: 16471561-16471626 [-]
dme-mir-311 2R: 16471382-16471465 [-]
dme-mir-310 2R: 16471257-16471345 [-]

Mature sequence dme-miR-312-5p

Accession MIMAT0020838
Sequence

5 -  

ugguucgucacaagggcaauucu

 - 27

Get sequence
Deep sequencing 5435 reads, 40 experiments
Evidence not experimental
Predicted targets

Mature sequence dme-miR-312-3p

Accession MIMAT0000404
Previous IDs dme-miR-312
Sequence

43 -  

uauugcacuugagacggccuga

 - 64

Get sequence
Deep sequencing 39596 reads, 44 experiments
Evidence experimental; cloned [1], Northern [1], 454 [2-3], Solexa [3]
Predicted targets

References

1
PMID:12919683 "The small RNA profile during Drosophila melanogaster development" Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T Dev Cell. 5:337-350(2003).
2
PMID:17989254 "Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs" Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC Genome Res. 17:1850-1864(2007).
3
PMID:17989255 "Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes" Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M Genome Res. 17:1865-1879(2007).