miRBase entry: dme-mir-315

Stem-loop dme-mir-315


Accession
MI0000427
Description
Drosophila melanogaster dme-mir-315 precursor miRNA
Gene family
MIPF0000141; mir-315

Literature search
5 open access papers mention dme-mir-315
(10 sentences)

Sequence

246620 reads, 3566 reads per million, 46 experiments
cacuuauauaaUUUUGAUUGUUGCUCAGAAAGCccucauuguuuaccaguuggCUUUCGAGCAAUAAUUGAAACCagauaagug
(((((((....(((((((((((((((.(((((((...((((.....)))).))))))))))))))))))))))....)))))))

Structure
       auaa               A       cuc    u 
cacuuau    UUUUGAUUGUUGCUC GAAAGCc   auug u
|||||||    ||||||||||||||| |||||||   |||| u
gugaaua    AAAGUUAAUAACGAG CUUUCgg   ugac a
       gaCC               -       --u    c 


Annotation confidence High
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Comments
Lai et al. predicted this sequence based on conservation in D. pseudoobscura, but did not verify expression [2]. Aravin et al. describe identification and mapping of the ends of the excised sequence by cloning [1].

Genome context
chr3L: 18860971-18861054 [+]

Database links

Mature dme-miR-315-5p

Accession MIMAT0000407
Description Drosophila melanogaster dme-miR-315-5p mature miRNA
Sequence 12 - UUUUGAUUGUUGCUCAGAAAGC - 33
Evidence experimental
cloned [1], 454 [3-4], Illumina [4]
Database links
Predicted targets

Mature dme-miR-315-3p

Accession MIMAT0020841
Description Drosophila melanogaster dme-miR-315-3p mature miRNA
Sequence 54 - CUUUCGAGCAAUAAUUGAAACC - 75
Evidence not_experimental
Database links

References

  1. PubMed ID: 12919683
    The small RNA profile during Drosophila melanogaster development
    "Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T"
    "Dev Cell (2003) 5:337-350

  2. PubMed ID: 17989254
    Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs
    "Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC"
    "Genome Res (2007) 17:1850-1864

  3. PubMed ID: 17989255
    Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes
    "Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M"
    "Genome Res (2007) 17:1865-1879

  4. PubMed ID: 12844358
    Computational identification of Drosophila microRNA genes
    "Lai EC, Tomancak P, Williams RW, Rubin GM"
    "Genome Biol (2003) 4:R42