Stem-loop sequence hsa-mir-127

Accession

MI0000472 (change log)

Symbol

HGNC:MIR127

Description

Homo sapiens miR-127 stem-loop

Gene family

MIPF0000080; mir-127

Literature search

154 open access papers mention hsa-mir-127
(919 sentences)

Stem-loop

   -----  u   ca   ucu      ugcug         g  c      --  a 
5'      ug gau  cug   ccagcc     aagcucaga gg ucugau  uc g
        || |||  |||   ||||||     ||||||||| || ||||||  || a
3'      ac cug  ggc   ggucgg     uucgagucu cc aggcua  ag a
   cuacu  u   aa   --u      -----         g  u      cu  a 

Deep sequencing

220520 reads, 396 reads per million, 134 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

This miRNA sequence is predicted based on homology to a verified miRNA from mouse [1]. The expression of this miRNA was later confirmed by cloning in human cells [2,3].

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chr14: 100882979-100883075 [+]
• Overlapping transcripts:

  sense

  OTTHUMT00000414309 ; WDR25-007; intron 1

  OTTHUMT00000414313 ; WDR25-008; intron 1

  OTTHUMT00000414315 ; WDR25-005; intron 1

  OTTHUMT00000414316 ; WDR25-006; intron 1

  OTTHUMT00000414310 ; WDR25-002; intron 2

  OTTHUMT00000414311 ; WDR25-001; intron 2

  OTTHUMT00000414312 ; WDR25-004; intron 2

  OTTHUMT00000414314 ; WDR25-003; intron 2

  ENST00000555775 ; WDR25-007; intron 1

  ENST00000554492 ; WDR25-008; intron 1

  ENST00000557710 ; WDR25-005; intron 1

  ENST00000542471 ; WDR25-006; intron 1

  ENST00000554998 ; WDR25-002; intron 2

  ENST00000402312 ; WDR25-001; intron 2

  ENST00000335290 ; WDR25-004; intron 2

  ENST00000554175 ; WDR25-003; intron 2

Clustered miRNAs

< 10kb from hsa-mir-127

• hsa-mir-337: chr14: 100874493-100874585 [+]
• hsa-mir-665: chr14: 100875033-100875104 [+]
• hsa-mir-431: chr14: 100881007-100881120 [+]
• hsa-mir-433: chr14: 100881886-100881978 [+]
• hsa-mir-127: chr14: 100882979-100883075 [+]
• hsa-mir-432: chr14: 100884483-100884576 [+]
• hsa-mir-136: chr14: 100884702-100884783 [+]

Database links

• EntrezGene:MIR127
• HGNC:MIR127
• RNAcentral:URS0000007340_9606
• miRBase Tracker:MI0000472

Mature sequence hsa-miR-127-5p

• Accession: MIMAT0004604
• Sequence: 23 - cugaagcucagagggcucugau - 44
• Deep sequencing: 13044 reads, 118 experiments
• Evidence: experimental; cloned [3-4]
• Database links:
  • RNAcentral:URS00000A33DB_9606
• Predicted targets:
  • TargetMiner:hsa-miR-127-5p
  • TargetScanVert:hsa-miR-127-5p
  • miRDB:hsa-miR-127-5p
  • microrna.org:hsa-miR-127-5p

Mature sequence hsa-miR-127-3p

• Accession: MIMAT0000446
• Previous IDs: hsa-miR-127
• Sequence: 57 - ucggauccgucugagcuuggcu - 78
• Deep sequencing: 207374 reads, 133 experiments
• Evidence: experimental; cloned [2-3]
• Database links:
  • RNAcentral:URS00001E3DAA_9606
• Predicted targets:
  • TargetMiner:hsa-miR-127-3p
  • TargetScanVert:hsa-miR-127-3p
  • miRDB:hsa-miR-127-3p
  • microrna.org:hsa-miR-127-3p

References

1. PMID:12007417 "Identification of tissue-specific microRNAs from mouse" Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T Curr Biol. 12:735-739(2002).
2. PMID:15891114 "Clustering and conservation patterns of human microRNAs" Altuvia Y, Landgraf P, Lithwick G, Elefant N, Pfeffer S, Aravin A, Brownstein MJ, Tuschl T, Margalit H Nucleic Acids Res. 33:2697-2706(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:18230126 "New miRNAs cloned from neuroblastoma" Afanasyeva EA, Hotz-Wagenblatt A, Glatting KH, Westermann F BMC Genomics. 9:52(2008).