Stem-loop sequence mmu-mir-218-1

AccessionMI0000700
Symbol MGI:Mir218-1
DescriptionMus musculus miR-218-1 stem-loop
Gene family MIPF0000026; mir-218
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled mir-218_microRNA_precursor_family. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

miR-218 microRNA precursor is a small non-coding RNA that regulates gene expression by antisense binding. miR-218 appears to be a vertebrate specific microRNA and has now been predicted and experimentally confirmed in a wide range of vertebrate species. The extents of the hairpin precursors are not known. In this case the mature sequence in excised from the 5'arm of the hairpin. miR-218, along with miR-585, has been found to be silenced by DNA methylation in oral squamous cell carcinoma. It is also downregulated in Nasopharyngeal carcinoma, with artificially-induced expression serving to slow tumour growth. miR-218 has also been found to have tumour suppressing qualities in bladder cancer cells.

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Stem-loop
   gugauaaugga   a   u      u         cu        ---g   gcg 
5'            gcg gau uucugu gugcuugau  aaccaugu    cuu   a
              ||| ||| |||||| |||||||||  ||||||||    |||   g
3'            cgc cug aaggua cacgaacug  uugguaca    gag   g
   acaucuuucga   a   c      c         cc        aaaa   uau 
Get sequence
Deep sequencing
341837 reads, 1.91e+03 reads per million, 81 experiments
Confidence
Feedback: Do you believe this miRNA is real?
Comments

Mouse miR-218 is predicted [2] based on homology to a reported miR from human (MI0000294) [1]. Its expression was later verified by cloning [3].

Genome context
Coordinates (GRCm38) Overlapping transcripts
chr5: 48223942-48224051 [+]
sense
OTTMUST00000096636 ; Slit2-013; intron 1
OTTMUST00000096653 ; Slit2-016; intron 8
OTTMUST00000096559 ; Slit2-003; intron 14
OTTMUST00000096561 ; Slit2-001; intron 14
OTTMUST00000096560 ; Slit2-002; intron 15
OTTMUST00000096578 ; Slit2-008; intron 15
OTTMUST00000096562 ; Slit2-006; intron 16
OTTMUST00000096577 ; Slit2-007; intron 16
ENSMUST00000172824 ; Slit2-013; intron 1
ENSMUST00000174487 ; Slit2-016; intron 8
ENSMUST00000033967 ; Slit2-003; intron 14
ENSMUST00000170109 ; Slit2-001; intron 14
ENSMUST00000174313 ; Slit2-002; intron 15
ENSMUST00000173107 ; Slit2-008; intron 15
ENSMUST00000174421 ; Slit2-006; intron 16
ENSMUST00000173702 ; Slit2-007; intron 16
Database links

Mature sequence mmu-miR-218-5p

Accession MIMAT0000663
Previous IDsmmu-miR-218
Sequence

25 - 

uugugcuugaucuaaccaugu

 - 45

Get sequence
Deep sequencing211239 reads, 58 experiments
Evidence experimental; cloned [3], Illumina [4-5]
Validated targets
Predicted targets

Mature sequence mmu-miR-218-1-3p

Accession MIMAT0004665
Previous IDsmmu-miR-218-1*
Sequence

64 - 

aaacaugguuccgucaagcacc

 - 85

Get sequence
Deep sequencing224 reads, 30 experiments
Evidence experimental; cloned [3], Illumina [4-5]
Predicted targets

References

1
PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
2
3
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4
PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5
PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).