miRBase entry: cel-mir-356a

Stem-loop cel-mir-356a


Accession
MI0000755
Description
Caenorhabditis elegans cel-mir-356a precursor miRNA
Gene family
MIPF0001209; mir-356

Literature search
2 open access papers mention cel-mir-356a
(3 sentences)

Sequence

215 reads, 0 reads per million, 13 experiments
uaaaguuuccuugagaacaauguggUUGAGCAACGCGAACAAAUCAucaauguaaauuucagcggauucguuacgacgugucucaccacaucguuaguaggauuucagaa
.......((((....(((.(((((((.(((..((((((((.((((.....((.......))...)))).))).)).)))..)))))))))).)))...))))........

Structure
-uaaaguu    ugag   a       U   CA   -  -   A    Aucaa  ua 
        uccu    aac auguggU GAG  ACG CG AAC AAUC     ug  a
        ||||    ||| ||||||| |||  ||| || ||| ||||     ||  a
        agga    uug uacacca cuc  ugc gc uug uuag     ac  u
aagacuuu    -uga   c       -   ug   a  a   c    --gcg  uu 


Annotation confidence Not enough data
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Comments
This miRNA was predicted by computational analysis of C. elegans and C. briggsae, and expression of the mature microRNA confirmed by PCR amplification, cloning and sequencing. The 5' end of the mature sequence ws mapped by PCR, but the 3' ends have not been experimentally determined.

Genome context
chrIII: 7860147-7860256 [+]
Clustered miRNAs
1 other miRNA is < 10 kb from cel-mir-356a
Name Accession Chromosome Start End Strand Confidence




Database links

Mature cel-miR-356a

Accession MIMAT0000698
Description Caenorhabditis elegans cel-miR-356a mature miRNA
Sequence 26 - UUGAGCAACGCGAACAAAUCA - 46
Evidence experimental
cloned [1], PCR [1]

References

  1. PubMed ID: 15317971
    Patterns of flanking sequence conservation and a characteristic upstream motif for microRNA gene identification
    "Ohler U, Yekta S, Lim LP, Bartel DP, Burge CB"
    "RNA (2004) 10:1309-1322