Stem-loop sequence hsa-mir-323a

Accession

MI0000807 (change log)

Previous IDs

hsa-mir-323

Symbol

HGNC:MIR323A

Description

Homo sapiens miR-323a stem-loop

Gene family

MIPF0000018; mir-154

Literature search

45 open access papers mention hsa-mir-323a
(107 sentences)

Stem-loop

   ---uu      u g        g u     gcgc  u    uua 
5'      gguacu g agagaggu g ccgug    gu cgcu   u
        |||||| | |||||||| | |||||    || ||||    
3'      cuauga c uuucucca c ggcac    ca gcgg   u
   cuaau      - g        g u     auua  c    uau 

Deep sequencing

3721 reads, 1.79 reads per million, 112 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

This sequence is the predicted homologue of a miRNA cloned from rat neuronal tissue [1,2], later verified in human [3].

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chr14: 101025732-101025817 [+]
• Overlapping transcripts:

  antisense

  OTTHUMT00000414334 ; BEGAIN-007; intron 1

  OTTHUMT00000414329 ; BEGAIN-001; intron 2

  OTTHUMT00000414331 ; BEGAIN-005; intron 2

  OTTHUMT00000414332 ; BEGAIN-003; intron 2

  OTTHUMT00000414335 ; BEGAIN-006; intron 2

  OTTHUMT00000414337 ; BEGAIN-004; intron 2

  ENST00000557378 ; BEGAIN-007; intron 1

  ENST00000443071 ; BEGAIN-201; intron 1

  ENST00000355173 ; BEGAIN-001; intron 2

  ENST00000553553 ; BEGAIN-005; intron 2

  ENST00000556188 ; BEGAIN-003; intron 2

  ENST00000554140 ; BEGAIN-006; intron 2

  ENST00000554747 ; BEGAIN-004; intron 2

Clustered miRNAs

< 10kb from hsa-mir-323a

• hsa-mir-379: chr14: 101022066-101022132 [+]
• hsa-mir-411: chr14: 101023325-101023420 [+]
• hsa-mir-299: chr14: 101023794-101023856 [+]
• hsa-mir-380: chr14: 101025017-101025077 [+]
• hsa-mir-1197: chr14: 101025564-101025651 [+]
• hsa-mir-323a: chr14: 101025732-101025817 [+]
• hsa-mir-758: chr14: 101026020-101026107 [+]
• hsa-mir-329-1: chr14: 101026785-101026864 [+]
• hsa-mir-329-2: chr14: 101027100-101027183 [+]
• hsa-mir-494: chr14: 101029634-101029714 [+]
• hsa-mir-1193: chr14: 101030052-101030129 [+]
• hsa-mir-543: chr14: 101031987-101032064 [+]
• hsa-mir-495: chr14: 101033755-101033836 [+]

Database links

• EntrezGene:MIR323
• HGNC:MIR323A
• RNAcentral:URS000075AB81_9606
• miRBase Tracker:MI0000807

Mature sequence hsa-miR-323a-5p

• Accession: MIMAT0004696
• Previous IDs: hsa-miR-323-5p
• Sequence: 16 - aggugguccguggcgcguucgc - 37
• Deep sequencing: 603 reads, 28 experiments
• Evidence: experimental; cloned [3]
• Predicted targets:
  • TargetMiner:hsa-miR-323a-5p
  • TargetScanVert:hsa-miR-323a-5p
  • miRDB:hsa-miR-323a-5p
  • microrna.org:hsa-miR-323a-5p

Mature sequence hsa-miR-323a-3p

• Accession: MIMAT0000755
• Previous IDs: hsa-miR-323;hsa-miR-323-3p
• Sequence: 51 - cacauuacacggucgaccucu - 71
• Deep sequencing: 3109 reads, 112 experiments
• Evidence: experimental; cloned [3]
• Database links:
  • RNAcentral:URS00003CCAB4_9606
• Predicted targets:
  • TargetMiner:hsa-miR-323a-3p
  • TargetScanVert:hsa-miR-323a-3p
  • miRDB:hsa-miR-323a-3p
  • microrna.org:hsa-miR-323a-3p

References

1. PMID:14691248 "Identification of many microRNAs that copurify with polyribosomes in mammalian neurons" Kim J, Krichevsky A, Grad Y, Hayes GD, Kosik KS, Church GM, Ruvkun G Proc Natl Acad Sci U S A. 101:360-365(2004).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).