Stem-loop sequence rno-mir-300

Accession

MI0000971 (change log)

Description

Rattus norvegicus miR-300 stem-loop

Gene family

MIPF0000018; mir-154

Literature search

7 open access papers mention rno-mir-300
(36 sentences)

Stem-loop

      a           g  au             gc   a 
5' gcu cuugaagagag uu  ccuuuguguguuu  uuu c
   ||| ||||||||||| ||  |||||||||||||  |||  
3' cga gagcuucucuc aa  gggaacguauaag  aag g
      g           g  -c             ua   c 

Deep sequencing

371732 reads, 217 reads per million, 463 experiments

Confidence

Annotation confidence: not enough data

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Genome context

• Coordinates (Rnor_6.0; GCA_000001895.4): chr6: 133874152-133874230 [+]
• Overlapping transcripts:

  sense

  ENSRNOT00000053757 ; Mir300-201; exon 1

Clustered miRNAs

< 10kb from rno-mir-300

• rno-mir-494: chr6: 133864370-133864452 [+]
• rno-mir-679: chr6: 133864564-133864700 [+]
• rno-mir-1193: chr6: 133864698-133864817 [+]
• rno-mir-666: chr6: 133866523-133866621 [+]
• rno-mir-543: chr6: 133866660-133866739 [+]
• rno-mir-495: chr6: 133868288-133868367 [+]
• rno-mir-667: chr6: 133869570-133869661 [+]
• rno-mir-376c: chr6: 133872439-133872522 [+]
• rno-mir-376b: chr6: 133873095-133873180 [+]
• rno-mir-3595: chr6: 133873587-133873706 [-]
• rno-mir-376a: chr6: 133873605-133873686 [+]
• rno-mir-300: chr6: 133874152-133874230 [+]
• rno-mir-381: chr6: 133876615-133876675 [+]
• rno-mir-487b: chr6: 133877124-133877205 [+]
• rno-mir-3576: chr6: 133877812-133877926 [-]
• rno-mir-539: chr6: 133877832-133877907 [+]
• rno-mir-6331: chr6: 133878578-133878700 [+]
• rno-mir-544: chr6: 133879169-133879246 [+]

Database links

• EntrezGene:Mir300
• miRBase Tracker:MI0000971

Mature sequence rno-miR-300-5p

• Accession: MIMAT0004743
• Sequence: 6 - uugaagagagguuauccuuugu - 27
• Deep sequencing: 889 reads, 124 experiments
• Evidence: experimental; cloned [2-3], SOLiD [4]
• Predicted targets:
  • TargetScanVert:rno-miR-300-5p
  • miRDB:rno-miR-300-5p
  • microrna.org:rno-miR-300-5p

Mature sequence rno-miR-300-3p

• Accession: MIMAT0000902
• Previous IDs: rno-miR-300
• Sequence: 51 - uaugcaagggcaagcucucuuc - 72
• Deep sequencing: 370842 reads, 463 experiments
• Evidence: experimental; cloned [1-3], SOLiD [4]
• Predicted targets:
  • TargetScanVert:rno-miR-300-3p
  • miRDB:rno-miR-300-3p
  • microrna.org:rno-miR-300-3p

References

1. PMID:15345052 "Microarray analysis of microRNA expression in the developing mammalian brain" Miska EA, Alvarez-Saavedra E, Townsend M, Yoshii A, Sestan N, Rakic P, Constantine-Paton M, Horvitz HR Genome Biol. 5:R68(2004).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
3. PMID:17805466 "Cloning and identification of novel microRNAs from rat hippocampus" He X, Zhang Q, Liu Y, Pan X Acta Biochim Biophys Sin (Shanghai). 39:708-714(2007).
4. PMID:20403161 "Small RNA expression and strain specificity in the rat" Linsen SE, de Wit E, de Bruijn E, Cuppen E BMC Genomics. 11:249(2010).