miRBase entry: mmu-mir-215

Stem-loop mmu-mir-215


Accession
MI0000974
Symbol
MGI: Mir215
Description
Mus musculus mmu-mir-215 precursor miRNA
Gene family
MIPF0000063; mir-192

Literature search
29 open access papers mention mmu-mir-215
(277 sentences)

Sequence

70706 reads, 1085 reads per million, 91 experiments
agcucucagcaucaacgguguacaggagaAUGACCUAUGAUUUGACAGACcgugcagcuguguaugUCUGUCAUUCUGUAGGCCAAUauucuguaugucacugcuacuuaaa
.(((...))).....((((((((((((...((.(((((((..((((((((.(((((....))))))))))))).)).))))).))...)))))))...))))).........

Structure
agcucucagcaucaa     ---       gaA  A     -  UU        c     g 
               cggug   uacagga   UG CCUAU GA  UGACAGAC gugca c
               |||||   |||||||   || ||||| ||  |||||||| |||||  
               gucac   augucuu   AC GGAUG CU  ACUGUCUg uaugu u
------aaauucauc     ugu       aUA  C     U  -U        -     g 


Annotation confidence High
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Comments
This mouse miRNA was predicted by computational methods using conservation with human and Fugu rubripes sequences [1]. Expression of the excised miR has been validated in zebrafish, and the 5' end mapped by PCR. The 3' end was not experimentally determined. The mature sequence differs from the human miR-215 at A12->U, and its expression has not been verified in mouse.

Genome context
chr1: 185313581-185313692 [+]
Clustered miRNAs
1 other miRNA is < 10 kb from mmu-mir-215
Name Accession Chromosome Start End Strand Confidence




Database links

Mature mmu-miR-215-5p

Accession MIMAT0000904
Description Mus musculus mmu-miR-215-5p mature miRNA
Sequence 30 - AUGACCUAUGAUUUGACAGAC - 50
Evidence experimental
Illumina [2-3]
Database links
Predicted targets

Mature mmu-miR-215-3p

Accession MIMAT0017169
Description Mus musculus mmu-miR-215-3p mature miRNA
Sequence 67 - UCUGUCAUUCUGUAGGCCAAU - 87
Evidence experimental
Illumina [3]
Database links
Predicted targets

References

  1. PubMed ID: 12624257
    Vertebrate microRNA genes
    "Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP"
    "Science (2003) 299:1540

  2. PubMed ID: 20215419
    MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing
    "Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A"
    "Mol Hum Reprod (2010) 16:463-471

  3. PubMed ID: 20413612
    Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
    "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
    "Genes Dev (2010) 24:992-1009