miRBase entry: ath-MIR396b

Stem-loop ath-MIR396b


Accession
MI0001014
Description
Arabidopsis thaliana ath-MIR396b precursor miRNA
Gene family
MIPF0000047; MIR396

Literature search
26 open access papers mention ath-MIR396b
(271 sentences)

Sequence

ggucauacuuUUCCACAGCUUUCUUGAACUUucuuuuucauuuccauuguuuuuuucuuaaacaaaaguaagaagaaaaaaaacuuuaagauuaagcauuuuggaaGCUCAAGAAAGCUGUGGGAAAacaugaca
.(((((..(((((((((((((((((((.(((((((..((..........(((((((((((........)))))))))))..........))..))).......)))).)))))))))))))))))))..))))).

Structure
g     ac                   A    -------   uu  auuuccauug           aac 
 gucau  uuUUCCACAGCUUUCUUGA CUUu       cuu  uc          uuuuuuucuua   a
 |||||  ||||||||||||||||||| ||||       |||  ||          |||||||||||    
 cagua  AAAGGGUGUCGAAAGAACU Gaag       gaa  ag          aaaagaagaau   a
a     ca                   C    guuuuac   uu  aauuucaaaa           gaa 


Annotation confidence Not enough data
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Comments
This sequence belongs to the miR396 family of miRNAs, which are predicted to target mRNAs coding for Growth Regulating Factor (GRF) transcription factors, rhodenase-like proteins, and kinesin-like protein B [1]. The mature sequence reported in [2] is offset by 1 nt with respect to the sequence shown here.

Genome context
chr5: 13611798-13611932 [+]

Database links

Mature ath-miR396b-5p

Accession MIMAT0000945
Description Arabidopsis thaliana ath-miR396b-5p mature miRNA
Sequence 11 - UUCCACAGCUUUCUUGAACUU - 31
Evidence experimental
5'RACE [1], Northern [1-2], PCR [1], 454 [3], MPSS [3]

Mature ath-miR396b-3p

Accession MIMAT0031909
Description Arabidopsis thaliana ath-miR396b-3p mature miRNA
Sequence 107 - GCUCAAGAAAGCUGUGGGAAA - 127
Evidence not_experimental

References

  1. PubMed ID: 16954541
    MicroRNAs and other small RNAs enriched in the Arabidopsis RNA-dependent RNA polymerase-2 mutant
    "Lu C, Kulkarni K, Souret FF, MuthuValliappan R, Tej SS, Poethig RS, Henderson IR, Jacobsen SE, Wang W, Green PJ, Meyers BC"
    "Genome Res (2006) 16:1276-1288

  2. PubMed ID: 17182867
    A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana
    "Rajagopalan R, Vaucheret H, Trejo J, Bartel DP"
    "Genes Dev (2006) 20:3407-3425

  3. PubMed ID: 15345049
    Prediction and identification of Arabidopsis thaliana microRNAs and their mRNA targets
    Wang XJ, Reyes JL, Chua NH, Gaasterland T
    Genome Biol (2004) 5:R65

  4. PubMed ID: 15200956
    Computational identification of plant microRNAs and their targets, including a stress-induced miRNA
    "Jones-Rhoades MW, Bartel DP"
    "Mol Cell (2004) 14:787-799