Stem-loop sequence hsa-mir-449a

Accession

MI0001648

Previous IDs

hsa-mir-449

Symbol

HGNC:MIR449A

Description

Homo sapiens miR-449a stem-loop

Gene family

MIPF0000133; mir-449

Stem-loop

-c      ug     c       -    u          ugaa   g 
  ugugug  augag uggcagu guau guuagcuggu    uau u
  ||||||  ||||| ||||||| |||| ||||||||||    |||  
  auauac  uauuc gucguca cgua caaucggcua    gua g
ac      gu     u       a    -          --cg   a 

Deep sequencing

22 reads, 6 experiments

Comments

Xie et al. [1] refer to this sequence by the internal identifier MIR54. The sequence is unrelated to C. elegans mir-54 (MI0000025 ).

Genome context

• Coordinates (GRCh37.p5): chr5: 54466360-54466450 [-]
• Overlapping transcripts:

  sense

  OTTHUMT00000369716 ; CDC20B-005; intron 1

  OTTHUMT00000369716 ; CDC20B-005; intron 1

  OTTHUMT00000369716 ; CDC20B-005; intron 1

  OTTHUMT00000253908 ; CDC20B-001; intron 2

  OTTHUMT00000369715 ; CDC20B-004; intron 2

  OTTHUMT00000369714 ; CDC20B-003; intron 2

  OTTHUMT00000369713 ; CDC20B-002; intron 2

  OTTHUMT00000253908 ; CDC20B-001; intron 2

  OTTHUMT00000369715 ; CDC20B-004; intron 2

  OTTHUMT00000369714 ; CDC20B-003; intron 2

  OTTHUMT00000369713 ; CDC20B-002; intron 2

  OTTHUMT00000253908 ; CDC20B-001; intron 2

  OTTHUMT00000369715 ; CDC20B-004; intron 2

  OTTHUMT00000369714 ; CDC20B-003; intron 2

  OTTHUMT00000369713 ; CDC20B-002; intron 2

  ENST00000507931 ; CDC20B-005; intron 1

  ENST00000331730 ; CDC20B-201; intron 1

  ENST00000507931 ; CDC20B-005; intron 1

  ENST00000331730 ; CDC20B-201; intron 1

  ENST00000507931 ; CDC20B-005; intron 1

  ENST00000331730 ; CDC20B-201; intron 1

  ENST00000296733 ; CDC20B-001; intron 2

  ENST00000381375 ; CDC20B-004; intron 2

  ENST00000322374 ; CDC20B-003; intron 2

  ENST00000513180 ; CDC20B-002; intron 2

  ENST00000334206 ; CDC20B-202; intron 2

  ENST00000296733 ; CDC20B-001; intron 2

  ENST00000381375 ; CDC20B-004; intron 2

  ENST00000322374 ; CDC20B-003; intron 2

  ENST00000513180 ; CDC20B-002; intron 2

  ENST00000334206 ; CDC20B-202; intron 2

  ENST00000296733 ; CDC20B-001; intron 2

  ENST00000381375 ; CDC20B-004; intron 2

  ENST00000322374 ; CDC20B-003; intron 2

  ENST00000513180 ; CDC20B-002; intron 2

  ENST00000334206 ; CDC20B-202; intron 2

Clustered miRNAs

< 10kb from hsa-mir-449a

• hsa-mir-449c: chr5: 54468090-54468181 [-]
• hsa-mir-449b: chr5: 54466474-54466570 [-]
• hsa-mir-449a: chr5: 54466360-54466450 [-]

Database links

• ENTREZGENE: 554213;MIR449A
• HGNC: 27645;MIR449A

Mature sequence hsa-miR-449a

• Accession: MIMAT0001541
• Previous IDs: hsa-miR-449
• Sequence: 16 - uggcaguguauuguuagcuggu - 37
• Deep sequencing: 22 reads, 6 experiments
• Evidence: experimental; cloned [1-2]
• Validated targets:
  • MIRECORDS:hsa-miR-449a
  • MIRTARBASE:hsa-miR-449a
  • TARBASE:hsa-miR-449a
• Predicted targets:
  • DIANA-MICROT:hsa-miR-449a
  • MICRORNA.ORG:hsa-miR-449a
  • MIRDB:hsa-miR-449a
  • RNA22-HSA:hsa-miR-449a
  • TARGETMINER:hsa-miR-449a
  • TARGETSCAN-VERT:hsa-miR-449a

References

1. PMID:15735639 "Systematic discovery of regulatory motifs in human promoters and 3' UTRs by comparison of several mammals" Xie X, Lu J, Kulbokas EJ, Golub TR, Mootha V, Lindblad-Toh K, Lander ES, Kellis M Nature. 434:338-345(2005).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).