miRBase entry: ppy-mir-27a

Stem-loop ppy-mir-27a

Accession

MI0003046

Description

Pongo pygmaeus ppy-mir-27a precursor miRNA

Gene family

MIPF0000036; mir-27

Sequence

cugaggagcacggggcuuagcugcuugugagcaggguccacaccaaguugugUUCACAGUGGCUAAGUUCCGCCccccag
(((.((.(..(((((((((((((((.(((((((.((.(........))).))))))))))))))))))))))..))))))

Structure

   a  a ca               u       g  u cac 
cug gg g  cggggcuuagcugcu gugagca gg c   a
||| || |  ||||||||||||||| ||||||| || |    
gac cc c  GCCUUGAAUCGGUGA CACUUgu uu g   c
   -  - CC               -       g  - aac

Annotation confidence Not enough data

Do you think this miRNA is real?

Comments

Berezikov et al. used primers designed from human miRNA gene flanking sequence to amplify miRNA precursor regions in primates [1]. The expression of the mature miRNA was not validated.

Genome context

NW_002966782.1: 23150-23229 [-]

Clustered miRNAs

2 other miRNAs are < 10 kb from ppy-mir-27a

Name	Accession	Chromosome	Start	End	Strand	Confidence

Database links

Mature ppy-miR-27a

Accession	MIMAT0002748
Description	Pongo pygmaeus ppy-miR-27a mature miRNA
Sequence	53 - UUCACAGUGGCUAAGUUCCGCC - 74
Evidence	not_experimental

References

PubMed ID: 15652478
Phylogenetic shadowing and computational identification of human microRNA genes
"Berezikov E, Guryev V, van de Belt J, Wienholds E, Plasterk RH, Cuppen E"
"Cell (2005) 120:21-24