Stem-loop sequence mmu-mir-532

Accession

MI0003206 (change log)

Symbol

MGI:Mir532

Description

Mus musculus miR-532 stem-loop

Gene family

MIPF0000113; mir-188

Literature search

13 open access papers mention mmu-mir-532
(76 sentences)

Stem-loop

   c   uuu    u       c  u      a    a   cc  ugaca 
5'  aga   gcuu uucucuu ca gccuug gugu gga  gu     u
    |||   |||| ||||||| || |||||| |||| |||  ||     c
3'  ucu   cgaa gagagga gu cggaac caca ccu  ca     u
   c   -uc    c       c  u      c    c   cc  uuaau 

Deep sequencing

168276 reads, 314 reads per million, 108 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chrX: 7248402-7248497 [-]
• Overlapping transcripts:

  sense

  OTTMUST00000039775 ; Clcn5-003; intron 2

  OTTMUST00000039776 ; Clcn5-004; intron 3

  OTTMUST00000039777 ; Clcn5-005; intron 3

  ENSMUST00000128319 ; Clcn5-003; intron 2

  ENSMUST00000115746 ; Clcn5-004; intron 3

  ENSMUST00000132126 ; Clcn5-005; intron 3

Clustered miRNAs

< 10kb from mmu-mir-532

• mmu-mir-532: chrX: 7248402-7248497 [-]
• mmu-mir-188: chrX: 7247989-7248056 [-]
• mmu-mir-362: chrX: 7241982-7242046 [-]
• mmu-mir-501: chrX: 7241243-7241351 [-]

Database links

• EntrezGene:Mir532
• MGI:Mir532
• RNAcentral:URS000075BC73_10090
• miRBase Tracker:MI0003206

Mature sequence mmu-miR-532-5p

• Accession: MIMAT0002889
• Previous IDs: mmu-miR-532
• Sequence: 21 - caugccuugaguguaggaccgu - 42
• Deep sequencing: 152005 reads, 105 experiments
• Evidence: experimental; PCR [1], miRAP-cloned [2], cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS00004E8341_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-532-5p
  • miRDB:mmu-miR-532-5p
  • microrna.org:mmu-miR-532-5p

Mature sequence mmu-miR-532-3p

• Accession: MIMAT0004781
• Sequence: 58 - ccucccacacccaaggcuugca - 79
• Deep sequencing: 16259 reads, 103 experiments
• Evidence: experimental; cloned [3], Illumina [4-5]
• Database links:
  • RNAcentral:URS00004B4B85_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-532-3p
  • miRDB:mmu-miR-532-3p
  • microrna.org:mmu-miR-532-3p

References

1. "PCR-based expression analysis and identification of microRNAs" Lu DP, Read RL, Humphreys DT, Battah FM, Martin DIK, Rasko JEJ RNAi and Gene Silencing 1:44-49(2005).
2. PMID:16973894 "Mouse microRNA profiles determined with a new and sensitive cloning method" Takada S, Berezikov E, Yamashita Y, Lagos-Quintana M, Kloosterman WP, Enomoto M, Hatanaka H, Fujiwara S, Watanabe H, Soda M, Choi YL, Plasterk RH, Cuppen E, Mano H Nucleic Acids Res. 34:e115(2006).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).