Stem-loop sequence mmu-mir-547

Accession

MI0003523

Symbol

MGI:Mir547

Description

Mus musculus miR-547 stem-loop

Gene family

MIPF0000779; mir-547

Stem-loop

     a   a     ug           cc   uuaaca g 
gugug ugu ucacu  aggauguacca  cau      g a
||||| ||| |||||  |||||||||||  |||      |  
cacac aua aguga  uucuacauggu  gua      c a
     a   g     gu           uc   ------ a 

Deep sequencing

1470 reads, 37 experiments

Comments

The predominant miRNA cloned by Langraf et al. has a 3' terminal U residue, which is incompatible with the genome sequence [2].

Genome context

• Coordinates (GRCm38): chrX: 67988374-67988451 [-]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from mmu-mir-547

• mmu-mir-547: chrX: 67988374-67988451 [-]
• mmu-mir-201: chrX: 67988096-67988161 [-]

Database links

• ENTREZGENE: 723918;Mir547
• MGI: 3619434;Mir547

Mature sequence mmu-miR-547-5p

• Accession: MIMAT0017210
• Previous IDs: mmu-miR-547*
• Sequence: 12 - cacuugaggauguaccaccca - 32
• Deep sequencing: 14 reads, 8 experiments
• Evidence: experimental; Solexa [4]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-547-5p
  • MIRDB:mmu-miR-547-5p
  • RNA22-MMU:mmu-miR-547-5p

Mature sequence mmu-miR-547-3p

• Accession: MIMAT0003173
• Previous IDs: mmu-miR-547
• Sequence: 49 - cuugguacaucuuugagugag - 69
• Deep sequencing: 1453 reads, 36 experiments
• Evidence: experimental; cloned [1-2], Solexa [3-4]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-547-3p
  • MICRORNA.ORG:mmu-miR-547-3p
  • MIRDB:mmu-miR-547-3p
  • RNA22-MMU:mmu-miR-547-3p
  • TARGETSCAN-VERT:mmu-miR-547

References

1. PMID:16274478 "Identification of clustered microRNAs using an ab initio prediction method" Sewer A, Paul N, Landgraf P, Aravin A, Pfeffer S, Brownstein MJ, Tuschl T, van Nimwegen E, Zavolan M BMC Bioinformatics. 6:267(2005).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
3. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
4. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).