miRBase entry: bta-let-7i

Stem-loop bta-let-7i


Accession
MI0005065
Description
Bos taurus bta-let-7i precursor miRNA
Gene family
MIPF0000002; let-7

Literature search
53 open access papers mention bta-let-7i
(253 sentences)

Sequence

614624 reads, 3806 reads per million, 76 experiments
cuggcUGAGGUAGUAGUUUGUGCUGUUggucggguugugacauugcccgcuguggagauaacugcgcaagcuacugccuugcua
.((((.(((((((((((((((((.(((((.(((((.........)))))))........))).)))))))))))))))))))))

Structure
c    U                 U   --------  u     ugu 
 uggc GAGGUAGUAGUUUGUGC GUU        gg cgggu   g
 |||| ||||||||||||||||| |||        || |||||   a
 aucg uuccgucaucgaacgcg caa        uc gcccg   c
-    -                 u   uagaggug  -     uua 


Annotation confidence High
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Comments
The 5' terminal base of the mature let-7i sequence identified by Coutinho et al [1] is reported as C, which conflicts with the draft genome sequence that requires U as shown here. The 5' end reported by Gu et al. is shorter by 7 nts [2].

Genome context
chr5: 51387456-51387539 [-]

Database links

Mature bta-let-7i

Accession MIMAT0003851
Description Bos taurus bta-let-7i mature miRNA
Sequence 6 - UGAGGUAGUAGUUUGUGCUGUU - 27
Evidence experimental
cloned [1-3]

References

  1. PubMed ID: 17105755
    Discovery and profiling of bovine microRNAs from immune-related and embryonic tissues
    "Coutinho LL, Matukumalli LK, Sonstegard TS, Van Tassell CP, Gasbarre LC, Capuco AV, Smith TP"
    "Physiol Genomics (2007) 29:35-43

  2. PubMed ID: 17306260
    Identification and characterization of microRNAs from the bovine adipose tissue and mammary gland
    "Gu Z, Eleswarapu S, Jiang H"
    "FEBS Lett (2007) 581:981-988

  3. PubMed ID: 19267191
    Identification and characteristics of cattle microRNAs by homology searching and small RNA cloning
    "Long JE, Chen HX"
    "Biochem Genet (2009) 47:329-343