Stem-loop mmu-mir-544

Accession

MI0005555

Symbol

MGI: Mir544

Description

Mus musculus mmu-mir-544 precursor miRNA

Gene family

MIPF0000436; mir-544

Literature search

5 open access papers mention mmu-mir-544
(103 sentences)

Sequence

2137 reads, 68 reads per million, 38 experiments

caccuagggaUCUUGUUAAAAAGCAGAGUCUgauugaggggccaagAUUCUGCAUUUUUAGCAAGCUCucaagugaug
(((...((((.((((((((((((((((((((.............))))))))).))))))))))).))))..)))...

Structure

---   cua    U           -         gauug 
   cac   ggga CUUGUUAAAAA GCAGAGUCU     a
   |||   |||| ||||||||||| |||||||||     g
   gug   cuCU GAACGAUUUUU CGUCUUAga     g
gua   -aa    C           A         accgg 

Annotation confidence High

Comments

This sequence was identified as a miRNA candidate by Berezikov et al. using RAKE and MPSS techniques [1]. Expression was independently shown in human and rat [2].

Genome context

chr12: 109729325-109729402 [+]

Clustered miRNAs

16 other miRNAs are < 10 kb from mmu-mir-544

Mature mmu-miR-544-5p

• Accession: MIMAT0017282
• Description: Mus musculus mmu-miR-544-5p mature miRNA
• Sequence: 11 - UCUUGUUAAAAAGCAGAGUCU - 31
• Evidence: experimental ; Illumina [4]

Mature mmu-miR-544-3p

• Accession: MIMAT0004941
• Description: Mus musculus mmu-miR-544-3p mature miRNA
• Sequence: 47 - AUUCUGCAUUUUUAGCAAGCUC - 68
• Evidence: experimental ; RAKE [1], Illumina [3-4]

References

1. PubMed ID: 17604727
   A mammalian microRNA expression atlas based on small RNA library sequencing
   "Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M"
   "Cell (2007) 129:1401-1414


2. PubMed ID: 20215419
   MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing
   "Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A"
   "Mol Hum Reprod (2010) 16:463-471


3. PubMed ID: 20413612
   Mammalian microRNAs: experimental evaluation of novel and previously annotated genes
   "Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP"
   "Genes Dev (2010) 24:992-1009


4. PubMed ID: 16954537
   Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis
   "Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E"
   "Genome Res (2006) 16:1289-1298