Stem-loop sequence hsa-mir-216b

Accession

MI0005569 (change log)

Symbol

HGNC:MIR216B

Description

Homo sapiens miR-216b stem-loop

Gene family

MIPF0000054; mir-216

Literature search

57 open access papers mention hsa-mir-216b
(175 sentences)

Stem-loop

   --g       gaa         a  --caaa      g  ac 
5'    cagacug   aaucucugc gg      ugugau uc  u
      |||||||   ||||||||| ||      |||||| ||   
3'    gucugac   uuagagaug cc      acacua ag  g
   aca       auc         c  auucac      a  ga 

Deep sequencing

963 reads, 5.88 reads per million, 85 experiments

Confidence

Annotation confidence: not enough data

Feedback: Do you believe this miRNA is real?

Comments

This sequence was identified as a miRNA candidate by Berezikov et al. using RAKE and MPSS techniques [1]. Expression was later confirmed by cloning [2].

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chr2: 56000714-56000795 [-]
• Overlapping transcripts:

  intergenic

Database links

• EntrezGene:MIR216B
• HGNC:MIR216B
• RNAcentral:URS000075EC67_9606
• miRBase Tracker:MI0005569

Mature sequence hsa-miR-216b-5p

• Accession: MIMAT0004959
• Sequence: 11 - aaaucucugcaggcaaauguga - 32
• Deep sequencing: 779 reads, 76 experiments
• Evidence: experimental; cloned [2], Illumina [3]
• Database links:
  • RNAcentral:URS000046CDA6_9606
• Predicted targets:
  • TargetScanVert:hsa-miR-216b-5p
  • miRDB:hsa-miR-216b-5p
  • microrna.org:hsa-miR-216b-5p

Mature sequence hsa-miR-216b-3p

• Accession: MIMAT0026721
• Sequence: 49 - acacacuuacccguagagauucua - 72
• Deep sequencing: 12 reads, 10 experiments
• Evidence: experimental; Illumina [3]
• Predicted targets:
  • TargetScanVert:hsa-miR-216b-3p
  • miRDB:hsa-miR-216b-3p

References

1. PMID:16954537 "Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis" Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E Genome Res. 16:1289-1298(2006).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
3. PMID:23034410 "Birth and expression evolution of mammalian microRNA genes" Meunier J, Lemoine F, Soumillon M, Liechti A, Weier M, Guschanski K, Hu H, Khaitovich P, Kaessmann H Genome Res. 23:34-45(2013).