Stem-loop sequence hsa-mir-200a

Accession

MI0000737 (change log)

Symbol

HGNC:MIR200A

Description

Homo sapiens miR-200a stem-loop

Gene family

MIPF0000019; mir-8

Literature search

692 open access papers mention hsa-mir-200a
(3904 sentences)

Stem-loop

   c    - c   g        -            -----------     a 
5'  cggg c ccu ugagcauc uuaccggacagu           gcugg u
    |||| | ||| |||||||| ||||||||||||           ||||| u
3'  gccc g gga acuuguag aauggucuguca           cgacc u
   c    a u   a        c            caaucucaguu     c 

Deep sequencing

1216765 reads, 2.31e+03 reads per million, 155 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

miR-200a was cloned from mouse kidney tissue [1], and expression later confirmed by cloning in human [3].

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chr1: 1167863-1167952 [+]
• Overlapping transcripts:

  sense

  OTTHUMT00000005071 ; B3GALT6-001; 3'UTR (exon 1)

  ENST00000379198 ; B3GALT6-001; 3'UTR (exon 1)

Clustered miRNAs

< 10kb from hsa-mir-200a

• hsa-mir-200b: chr1: 1167104-1167198 [+]
• hsa-mir-200a: chr1: 1167863-1167952 [+]
• hsa-mir-429: chr1: 1169005-1169087 [+]

Database links

• EntrezGene:MIR200A
• HGNC:MIR200A
• RNAcentral:URS000075A884_9606
• miRBase Tracker:MI0000737

Mature sequence hsa-miR-200a-5p

• Accession: MIMAT0001620
• Previous IDs: hsa-miR-200a*
• Sequence: 16 - caucuuaccggacagugcugga - 37
• Deep sequencing: 16269 reads, 114 experiments
• Evidence: experimental; cloned [3-4]
• Database links:
  • RNAcentral:URS000023B77E_9606
• Predicted targets:
  • TargetMiner:hsa-miR-200a-5p
  • TargetScanVert:hsa-miR-200a-5p
  • miRDB:hsa-miR-200a-5p
  • microrna.org:hsa-miR-200a-5p

Mature sequence hsa-miR-200a-3p

• Accession: MIMAT0000682
• Previous IDs: hsa-miR-200a
• Sequence: 54 - uaacacugucugguaacgaugu - 75
• Deep sequencing: 1200448 reads, 153 experiments
• Evidence: experimental; cloned [3-5]
• Database links:
  • RNAcentral:URS000008DA94_9606
• Predicted targets:
  • TargetMiner:hsa-miR-200a-3p
  • TargetScanVert:hsa-miR-200a-3p
  • miRDB:hsa-miR-200a-3p
  • microrna.org:hsa-miR-200a-3p

References

1. PMID:12554859 "New microRNAs from mouse and human" Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:15891114 "Clustering and conservation patterns of human microRNAs" Altuvia Y, Landgraf P, Lithwick G, Elefant N, Pfeffer S, Aravin A, Brownstein MJ, Tuschl T, Margalit H Nucleic Acids Res. 33:2697-2706(2005).
4. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
5. PMID:17616659 "Patterns of known and novel small RNAs in human cervical cancer" Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).