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miRBase |
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![]() 6 publications mentioning dme-mir-4Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-4. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary. |
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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-24-1, hsa-mir-24-2, dme-mir-1, dme-mir-2a-1, dme-mir-2a-2, dme-mir-2b-1, dme-mir-2b-2, dme-mir-3, dme-let-7, dme-mir-2c, hsa-let-7g, hsa-let-7i, hsa-mir-1-2, hsa-mir-15b, hsa-mir-1-1
Tg-miR-4 and other non-polysomal miRNA may regulate target mRNAs at later stages of parasite differentiation (e. g. bradyzoite); alternatively, they might not be involved in translation control (data not shown), as has been recently shown for a class of Arabidopsis miRNA [41] that use cleavage-competent and/or cleavage-resistant target sites found in specific non-coding RNAs to initiate the production of trans-acting (tasi)RNAs via the action of RDR6 [42].
[score:8]
MIR-60, together with MIR-4, also constitute the two most diversified Tg-MIRNA gene families (with 8 distinct members in each) among the 14 families identified with high confidence (Figure S2B and S3B).
[score:1]
Likewise Tg-miR-4, -49 and -60 were more abundant in type I strain, whereas Tg-miR-40 and -56 were clearly more abundant in type II.
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Number of reads: Tg-proto-miR-1 (158 reads), Tg-proto-miR-2 (109 reads), Tg-proto-miR-3 (138 reads) and Tg-proto-miR-4 (107 reads).
[score:1]
Not all Tg-miRNA, however, were found associated to polysomes, and this was notably the case of Tg-miR-4 (Figure 4E).
[score:1]
Tg-miR-4 and -43, and as well Tg-rdsRNA-17 and -28 were indeed detected in the HaFlag- Tg-AGO immuno-precipitates but not in control immuno-precipitates (Figure 7A), indicating that Tg-AGO is a common effector of both types of sRNAs.
[score:1]
Thus, normalized to the Tg-tRNA [Ala] signal, the miR-4 signal was six fold greater in type I than it was in types II and III (Figure 4A).
[score:1]
Figure S3 Tg-microRNA-4 family.
[score:1]
RNAs isolated from the immunoprecipitates were probed for Tg-miR-4, Tg-miR-43, Tg-rdsRNA-17 and Tg-rdsRNA-28.
[score:1]
[1 to 20 of 9 sentences]
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Other miRNAs from this paper: dme-mir-2a-1, dme-mir-2a-2, dme-mir-2b-1, dme-mir-2b-2, dme-mir-6-1, dme-mir-6-2, dme-mir-6-3, dme-mir-7, dme-mir-10, dme-mir-11, dme-mir-13a, dme-mir-13b-1, dme-mir-13b-2, dme-mir-184, dme-mir-277, dme-mir-133, dme-mir-279, dme-mir-124, dme-mir-79, dme-mir-210, dme-mir-285, dme-mir-286, dme-bantam, dme-mir-304, dme-let-7, dme-mir-125, dme-mir-310, dme-mir-2c
Furthermore, many targets of Notch signaling were also predicted as targets of the Bearded-box microRNAs miR-4 and miR-79 (E(spl)m5, Bearded, E(spl)mγ, and Tom) and of the K-box microRNAs miR-2 and miR-11 (E(spl)m5, E(spl)m2, E(spl)mδ, and E(spl)m3), consistent with previous observations [27].
[score:5]
The same microRNA is thought to repress bagpipe [14], which ranks second in the list of miR-4 target predictions (S3).
[score:3]
This gene is again very high scoring (15.6) and ranks second in the list of miR-4 target predictions (setting S1).
[score:3]
The Notch gene Bearded is recovered as a target of miR-4 (or miR-79, equivalently).
[score:3]
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Other miRNAs from this paper: dme-mir-2a-1, dme-mir-2a-2, dme-mir-2b-1, dme-mir-2b-2, dme-mir-7, dme-mir-79, dme-bantam, dme-mir-2c
We also examined expression of a different reporter transgene, GMR > eGFP:: Brdmut, which has its miR-4/-79 binding sites mutated in the Brd 3′UTR.
[score:3]
A GMR > eGFP:: Brd fragment, which had its miR-4 binding site seeds changed from AGCTTTA to ATAGGGA, was cloned into a pPelican vector with its mini- white marker gene deleted.
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Binding sites for miR-4/-79 (red) and miR-7 (blue) are indicated in the Brd 3′UTR.
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This UTR has been experimentally demonstrated to contain three binding sites for miR-4/miR-79 and one binding site for miR-7 (Lai et al. 2005).
[score:1]
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Other miRNAs from this paper: cel-let-7, cel-mir-1, cel-mir-2, cel-mir-43, cel-mir-75, cel-mir-79, cel-mir-80, cel-mir-81, cel-mir-82, cel-mir-86, cel-mir-87, dme-mir-1, dme-mir-2a-1, dme-mir-2a-2, dme-mir-2b-1, dme-mir-2b-2, cel-mir-124, cel-mir-233, cel-mir-248, cel-mir-273, dme-mir-184, dme-mir-277, dme-mir-124, dme-mir-79, dme-mir-87, dme-bantam, dme-let-7, dme-mir-2c, cbr-let-7, cbr-mir-1, cbr-mir-43, cbr-mir-75, cbr-mir-79, cbr-mir-80, cbr-mir-81, cbr-mir-82, cbr-mir-86, cbr-mir-87, cbr-mir-124a, cbr-mir-233, cbr-mir-248, cel-mir-356a, dps-bantam, dps-let-7, dps-mir-1, dps-mir-2a-1, dps-mir-2a-2, dps-mir-2b-1, dps-mir-2b-2, dps-mir-2c, dps-mir-4, dps-mir-79, dps-mir-87, dps-mir-124, dps-mir-184, dps-mir-277, cbr-mir-356, cel-mir-356b, cbr-mir-2, cbr-mir-124b, cbr-mir-124c
The target sets for the miRNAs targeting the Brd box, miR-4 and miR-79, are also enriched with genes involved in the Notch signaling pathway (p < 10 [−5] in both cases).
[score:5]
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Other miRNAs from this paper: dme-mir-5, dme-mir-6-3, dme-mir-276a, dme-mir-282, dme-mir-100, dme-mir-286, dme-mir-87, dme-let-7, dme-mir-125, dme-mir-309, dme-mir-981, dme-mir-927, dme-mir-137, bdo-mir-100, bdo-let-7, bdo-mir-276a, bdo-mir-981, bdo-mir-137, bdo-mir-125, bdo-mir-5b-1, bdo-mir-4, bdo-mir-5a, bdo-mir-927, bdo-mir-87, bdo-mir-5b-2, bdo-mir-282, bdo-mir-286, bdo-mir-11593, bdo-mir-11594, bdo-mir-11595, bdo-mir-309b, bdo-mir-309a, bdo-mir-11596, bdo-mir-11597
Finally, of the four conserved genomic miRNA clusters identified in B. dorsalis, only the mir-309-6 cluster (dme-mir-4, dme-mir-6-3, dme-mir-5, dme-mir-286, and dme-mir-309) had an analogous expression pattern across life stages.
[score:3]
Six out of the 11 novel B. dorsalis miRNAs belong to this group, as did miRNAs belonging to the D. melanogaster miR-309-6 cluster (i. e. mir-4, mir-5 and mir-286), (Fig. 2, group 3).
[score:1]
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Other miRNAs from this paper: dme-mir-3, dme-mir-5, dme-mir-6-1, dme-mir-6-2, dme-mir-6-3, dme-mir-281-1, dme-mir-34, dme-mir-92b, dme-mir-286, dme-mir-309, dme-mir-310, dme-mir-193, dme-mir-988, dme-mir-1000
Of particular interest is the miRNA 309 cluster, which, in addition to mir-309, comprises mir-286, mir-3, mir-4, mir-5 and mir-6. The latter gives rise to three alternative stem-loop configurations with similar conserved seed regions [31, 32].
[score:1]
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