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10 publications mentioning dme-mir-6-3

Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-6-3. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 40
It is noteworthy that since mRFP and miG-1 expressions rely on the same ubiquitin promoter, the level of mRFP protein directly reflects the expression level of miG-1. As a negative control, we used the miR-5-miR-6.1-mRFP construct that is devoid of GFP -targeting miRNA (Fig. 1A, left panel). [score:8]
The miG-1-miR-6.1–mRFP construct (right) expresses miR-6.1 as well as miG-1 which targets the GFP mRNA expressed from pUbi-GFP with perfect complementarity. [score:7]
The GFP protein was also undetectable upon co-transfections of miG-1-miR-6.1-mRFP, pUbi-GFP with a vector expressing the HA-tagged B2 VSR (Fig. 1B, compare lane 4 with lane 5). [score:3]
The ubiquitin promoter in the miG-1-miR-6.1-mRFP construct drives the expression of both the monomeric Red Fluorescent Protein and the stem-loop precursor sequences of miG-1 and miR-6.1 miRNAs inserted in an Rpl17 intron (Fig. 1A, right panel). [score:3]
The GFP protein was readily detected in western blot experiments upon co-transfection of Drosophila S2 cells with the miR-5-miR-6.1-mRFP control plasmid and the pUbi-GFP target plasmid (Fig. 1B, lane 1). [score:3]
Here, we set up an automiG-derivate system combining a miG-1-miR-6.1-mRFP silencing vector and a pUbi-GFP target plasmid (Fig. 1A). [score:3]
In contrast, the GFP protein was barely detectable in the presence of the miG-1-miR-6.1-mRFP silencing plasmid (Fig. 1B, lanes 5 and 8), indicating that miG-1 efficiently silences GFP expression. [score:3]
Control plasmid with non-cognate miR-5 and miR-6 was used as a control for miRNA target specificity and α-tubulin is the loading control. [score:3]
The miR-5-miR-6.1-mRFP construct (left), expresses miR-5 and miR-6.1 without complementarity to the GFP and was used as a control. [score:3]
edu/) and carrying an ubiquitin promoter and a mRFP gene reporter to generate the miR-5-miR-6.1-mRFP and miG-1-miR-6.1-mRFP plasmids respectively (Fig. 1A). [score:1]
The pENTRY-miR-5-miR-6.1 and pENTRY-miG-1-miR-6.1 plasmids [34] were recombined with the Gateway pUWR destination vector (a kind gift from Clara Moch and Jean-Rene Huynh) described at the Drosophila Genome Ressource Center (https://dgrc. [score:1]
The resulting pENTRY-3C_miG-2 construct was then recombined with pAWH destination vector to give pAct-miG-2. To generate the automiW construct, DNA oligonucleotides containing the backbone sequences of pre-miR-5 and pre-miR6-1 [34] and 22 nt perfectly complementary to exons 6 and 5 of the white gene, respectively, in place of the mature miR-5 and miR-6.1 sequences (bolded in S1 Table) were annealed and cloned in place of miG-1 and miG-2, respectively, in the pENTRY-3C_miG-1_miG-2 construct [34]. [score:1]
The resulting pENTRY-3C_miG-2 construct was then recombined with pAWH destination vector to give pAct-miG-2. To generate the automiW construct, DNA oligonucleotides containing the backbone sequences of pre-miR-5 and pre-miR6-1 [34] and 22 nt perfectly complementary to exons 6 and 5 of the white gene, respectively, in place of the mature miR-5 and miR-6.1 sequences (bolded in S1 Table) were annealed and cloned in place of miG-1 and miG-2, respectively, in the pENTRY-3C_miG-1_miG-2 construct [34]. [score:1]
[1 to 20 of 13 sentences]
2
[+] score: 15
Other miRNAs from this paper: dme-mir-5, dme-mir-6-1, dme-mir-6-2, dme-mir-7, dme-bantam
Note that the mir-5/mir-6 cluster is mostly expressed during embryogenesis and that only very few reads matching these miRNAs are recovered from the S2R+ and adult flies libraries. [score:3]
In contrast, S2R+ cells stably transfected with the miR5-6.1-GFP construct express mature miR-5 and miR-6.1 miRNAs upon copper induction (Fig. 1C). [score:3]
Drosophila miR-5 and miR6.1 are not expressed in S2R+ cultured cells. [score:3]
mir-5 and mir-6.1 are light-blue shaded. [score:1]
A pENTR-3C_miG1_miG2 vector was then produced by replacing the EcoRI-mir-5-SphI and HindIII-mir-6-1-ClaI fragments in pENTR-3C_miR5-miR6 by EcoRI-miG1-SphI and HindIII-miG2-ClaI sequences, as depicted in Fig. 1A. [score:1]
Probes were AS-miR5 5′-CATATCACAACGATCGTTCCTTT-3′, AS-miR6 5′-AAAAAGAACAGCCACTGTGATA-3′, AS-miG1 5′-AGAACGGCATCAAGGTGAACTTC-3′, AS-miG2 5′-TGAAGGGCATCGACTTCAAGGA-3′, AS-miG1* 5′-ATGAAGTTCCCTTAATGCCGTT-3′, AS-miG2* 5′-TACCATTGAAACCGACGCCCCT-3′ AS-U6 5′-CGATTTTGCGTGTCATCCTTGC-3′. [score:1]
Bulges and mismatches were introduced in the stems of pre-miG-1 and pre-miG-2 to keep as much as possible the pre-miR-5 and pre-miR-6.1 backbone structures. [score:1]
Positions of mature miR-5 and miR-6.1 sequences are indicated with blue bars. [score:1]
A Gateway pENTR-3C vector (Invitrogen) was engineered to give rise to pENTR-3C_miR5-miR6. [score:1]
[1 to 20 of 9 sentences]
3
[+] score: 13
As qPCR experiments showed, the levels of pri-mir-6 and pri-mir-286 decreased after HS, apparently owing to downregulation of the entire 6–309 cluster. [score:4]
However, mature miR-5-5p, miR-286-3p and miR-6-3p were concurrently upregulated after HS. [score:4]
Using qPCR, we found that HS exposure led to a decrease in the expression (two- to 2.5-fold, p ≤ 0.05) of several selected pri-miRNAs, specifically, pri-mir-14, pri-mir-6, pri-mir-286, pri-mir-311 and pri-mir-308 in the w [1118] strain (figure 5 a). [score:3]
After a 24 h recovery period, pri-mir-14, pri-mir-6 and pri-mir-286 gradually returned to their original levels. [score:1]
The same applies to miR-6 and miR-286, which are encoded in the genome as miRNA cluster 6–309 and transcribed as a single pri-miRNA transcript. [score:1]
[1 to 20 of 5 sentences]
4
[+] score: 7
Other miRNAs from this paper: dme-mir-5, dme-mir-6-1, dme-mir-6-2, dme-mir-14, dme-mir-309
We found that target sets for the majority of our short-listed miRNAs (10 out of 17) show significant enrichment in our mixed decay class V (Figure 8e); this includes predicted targets for miR-6, miR-5 and miR-309, all of which belong to the miR-309 cluster, and is in agreement with the enrichment patterns of their experimentally validated targets (Figure 8c). [score:7]
[1 to 20 of 1 sentences]
5
[+] score: 6
Other miRNAs from this paper: dme-mir-6-1, dme-mir-6-2
We also generated flies expressing GMR -driven artificial microRNAs, using the mir-6.1 backbone, designed to target specific 21 bp sequences within echinus. [score:5]
In brief, 22 bp sequences complementary to echinus were substituted into the mir-6.1 precursor stem backbone at the position normally occupied by the mature mir-6 miRNA (C. H. Chen and B. A. Hay, unpublished). [score:1]
[1 to 20 of 2 sentences]
6
[+] score: 3
Finally, of the four conserved genomic miRNA clusters identified in B. dorsalis, only the mir-309-6 cluster (dme-mir-4, dme-mir-6-3, dme-mir-5, dme-mir-286, and dme-mir-309) had an analogous expression pattern across life stages. [score:3]
[1 to 20 of 1 sentences]
7
[+] score: 3
miR-6 is expressed at very low levels in S2 cells. [score:3]
[1 to 20 of 1 sentences]
8
[+] score: 1
For sickle we found one conserved site in all flies for miR-2, miR-13, and miR-6, which share the same nucleus. [score:1]
[1 to 20 of 1 sentences]
9
[+] score: 1
Of particular interest is the miRNA 309 cluster, which, in addition to mir-309, comprises mir-286, mir-3, mir-4, mir-5 and mir-6. The latter gives rise to three alternative stem-loop configurations with similar conserved seed regions [31, 32]. [score:1]
[1 to 20 of 1 sentences]
10
[+] score: 1
Ongoing work in our laboratory suggests, for instance, that the mir-6-3∼mir-309 cluster may be conserved beyond dipterans (Ninova, Ronshaugen and Griffiths-Jones; in preparation). [score:1]
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