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24 publications mentioning dme-mir-14

Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-14. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 165
Other miRNAs from this paper: dme-mir-317, dme-mir-981
We therefore introduced over expressed miR-14 (UAS miR-14) in flies having over expressed Ago-1. Interestingly, GMR-GAL4 driven over expression of miR-14 (UAS miR-14) under Ago-1 over expressed background (UAS Ago-1/ GMR-GAL4; UAS miR-14) successfully recovered Ago-1 over expression induced eye phenotype to normal (Fig 9III and S15 Fig). [score:11]
Ago-1 regulates miR-14 expression to sensitize cells for apoptosis and control organ developmentAs miRs are one of the crucial regulators of various cellular processes [42– 46], our interest was to see whether any change in their expression occurs under Ago-1 overexpression. [score:10]
Since miR-14 expression was extremely down regulated in Ago-1 overexpressed lines, we wanted to see whether it plays any role in reverting the organ shape and size to normal when it is over expressed under Ago-1 over expressed condition. [score:10]
Based on the present study in Drosophila it is resolved that, Ago-1 acts as a regulator of cell death, which is crucial for proper development of organs and might act as a tumour suppressor by inhibiting onco-miR-14 expression as well as by promoting JNK -dependent apoptosis. [score:9]
It has already been established as a suppressor of cell death, induced by hid, grim and rpr [47] Further, miR-14 has also been reported as a negative regulator of Dronc -dependent cell death and a regulator of Drice expression either directly or indirectly [47]. [score:9]
In Drosophila, miR-14 is expressed throughout development and has already been demonstrated as a negative cell death regulator at different stages of insect development by targeting pro apoptotic genes and caspases [47, 50]. [score:8]
A little up regulation of miR-14 expression in Ago-1 mutant line and high down regulation in Ago-1 over expressed group was noticed (Fig 9II). [score:7]
Apart from that, Ago-1 also negatively regulates the expression of apoptotic inhibitor micro RNA, miR-14. [score:6]
Ago-1 regulates miR-14 expression to sensitize cells for apoptosis and control organ development. [score:5]
Regulation of JNK pathway in one hand and miR-14 expression in other hand allows Ago-1 to take a better control with more perfection on developmental apoptosis. [score:5]
miR-14 regulates autophagy during developmental cell death by targeting ip3-kinase 2. Molecular cell. [score:5]
Ectopic expression of miR-14 successfully rescued the scalloped-GAL4 (sd-GAL4) driven Ago-1 over expressed phenotype of fruit fly wing. [score:5]
These observations positively suggest that Ago-1 regulates miR-14 expression to take a better hold in the control of developmental apoptosis in fruit fly. [score:5]
This ectopic expression of miR-14 by sd-GAL4 successfully rescued Ago-1 over expression induced wing phenotype (S16 Fig) to nearly normal phenotype. [score:5]
Up regulation of miR-14 in Ago-1 mutant and strong down regulation in Ago-1 over expression was noticed in quantitative PCR analysis. [score:5]
Our study revealed an interesting finding that Ago-1 promotes cell death and controls JNK phosphorylation by regulating Drosophila JNK kinase hep through its upstream activator, Tak1 to trigger the pathway in one hand and down regulate onco-miR miR-14 expression in another hand. [score:5]
Ectopic expression of miR-14 successfully rescued the GMR-GAL4 driven Ago-1 over expressed phenotype of fruit eye. [score:5]
S16 FigEctopic expression of miR-14 successfully rescued the scalloped-GAL4 (sd-GAL4) driven Ago-1 over expressed phenotype of fruit fly wing. [score:5]
0190548.g009 Fig 9 Ago-1 regulates miR-14 expression to get a better grip on the control of apoptosis. [score:4]
I. micro RNA micro array analysis clearly showed a significant down regulation of both miR-14 variant as a result of Ago-1 over expression. [score:4]
Ago-1 regulates miR-14 expression to get a better grip on the control of apoptosis. [score:4]
Therefore, miR-14 expression equips Ago-1 to apprehend developmental apoptosis in more disciplined manner. [score:4]
miR-14 is expressed throughout development of Drosophila. [score:4]
It also regulates miR-14 expression to control the cell death with better control. [score:4]
Further, flies carrying over expressed Ago-1 under sd- GAL4 (scalloped GAL4) resulted in flies having wings with reduced width compared to normal were also recovered with the introduction of over expressed miR 14 (sd-GAL4; UAS Ago-1; UAS miR-14) when driven with scalloped GAL4 in the wings of adult Drosophila. [score:4]
Several gene transcripts including Drice, Dcp-1 and grim possesses potential target sites for miR-14 binding (S14 Fig). [score:3]
Here, we’ve identified miR-14 as one of the key regulators of Ago-1 mediated developmental apoptotic process. [score:3]
This study sheds a new beam of light on the crosstalk and involvement of Ago-1 in the induction of cell death through JNK signaling in one hand and through regulating miR-14 in other hand (Fig 10) during organ development. [score:3]
For further validation of the involvement of miR-14 with Ago-1 regulated apoptotic cell death quantitative PCR analysis was also performed. [score:2]
Interestingly a huge down regulation of mir-14 which is related to apoptosis was observed (Fig 9I and S3 File). [score:2]
The Drosophila microRNA Mir-14 suppresses cell death and is required for normal fat metabolism. [score:2]
mRNAs of caspases and pro apoptotic genes with their miR-14 binding sites. [score:1]
S14 Fig The effector caspase, Drice carries the miR-14 binding site at the 3’UTR region of its mRNA; whereas DCP1 has the binding site for the same miR at the 5’ UTR end and pro apoptotic gene, grim mRNA posses the binding location at 3’UTR region. [score:1]
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2
[+] score: 59
Expression Patterns of sli-miR-14, sli-miR-2a and sli-bantam from S. lituraThe expression patterns of the putative miRNAs, sli-miR-14, sli-miR-2a and sli-bantam were also studied to make a further validation of this method. [score:5]
Sli-miR-14 was expressed strongly in all developmental stages of S. litura, but there were some differences among developmental stages. [score:5]
The relative expression levels of sli-miR-14 were 1.47-, 4.22-, 3.78-, 2.26-, 1.87-, 0.37- and 9.07-fold higher in the first instar, third instar, fourth instar, six instar, pre-pupa, pupa and adult than in eggs, respectively (Figure 5a), which indicated that sli-miR-14 expressed in pupal stage lower than in other stages, and especially strongly in adult stage. [score:5]
It has been clearly stated that cell autonomous anti-apoptotic activity mediated by miR-14 and miR-2a [12, 27], miR-14 also plays critical role in molting process [25], indicating miRNAs are involved in strict developmental regulation in insects. [score:3]
Three known miRNAs, miR-14, miR-2a and bantam, play important roles in the developmental stages in D. melanogaster [23, 24], such as regulating steroid hormone signaling [25] and apoptosis [12, 26, 27]. [score:3]
The expression patterns of the putative miRNAs, sli-miR-14, sli-miR-2a and sli-bantam were also studied to make a further validation of this method. [score:3]
Moreover, expression patterns analysis indicated that the highly conserved miRNAs, both miR-14 and miR-2a, could be detected successfully by real-time quantitative PCR, which confirms that stem-loop RT-PCR can be used not only for quantification of miRNAs, but also for identifying highly conserved miRNAs in non-mo del insects. [score:3]
Expression Patterns of sli-miR-14, sli-miR-2a and sli-bantam from S. litura. [score:3]
The homologues of miR-14, miR-2a and bantam were cloned from Spodoptera litura, a prevalent agriculture pest in China, by stem-loop RT-PCR, and their expression patterns in different developmental stages were also investigated to confirm the results of sequence analysis. [score:2]
However, the expression patterns of sli-miR-14 and sli-miR-2a, two highly conserved miRNAs in insect, could be obtained in this assay. [score:2]
Two gene fragments of S. frugiperda (FP355748.1 and FP352735.1) were found in EST Database (NCBI) by homologous searching according to the sequence of pupative sli-miR-14. [score:1]
Results showed the putative pre-sli-miR-14 shared 83.8% and 73% homology to pre-bmo-miR-14 and pre-dme-miR-14 respectively (Figure 2b). [score:1]
Results showed that putative pre-miR-14 and pre-miR-2a of S. litura can form stable stem-loop structures (initial Δ G = −41.80 and Δ G = −41.20 respectively) and are highly homologous to those of B. mori and D. melanogaster, indicating they are the precursors of sli-miR-14 and sli-miR-2a (Figure 4). [score:1]
Although the identification of miRNAs in non-mo del insects with stem-loop RT-PCR is limited by 5′ region of putative miRNA, large amounts of miRNAs that are highly conserved, such as miR-14 and miR-2a, can be simply cloned by this method. [score:1]
MiR-14 and miR-2a are more conserved members of miRNAs family than bantam in mo del insects including D. melanogaster and B. mori (Table S1). [score:1]
Genomic DNA Isolation and the Amplification of pre-miR-14 and pre-miR-2a from S. litura. [score:1]
The putative homologue of the three miRNAs in S. litura, namely sli-miR-14, sli-miR-2a and sli-bantam were cloned by a stem-loop RT-PCR technique. [score:1]
Amplification and Identification of sli-miR-14, sli-miR-2a and sli-bantam from S. lituraTo identify the availability of stem-loop RT-PCR technology for cloning conserved miRNAs from non-mo del insects, the sequences of miR-14, miR-2a and bantam in mo del insects were searched from miRBase (Table S1). [score:1]
When Group 1 was used for amplification of miR-14, miR-2a and bantam, PCR products were 86 bp, 89 bp, 89 bp, respectively (Figure 1a); when Group 2 was used, PCR products were 76 bp, 79 bp and 79 bp in length, respectively (Figure 1b). [score:1]
Blast procedure had been performed to analyze the homology among the putative pre-miR-14 in S. litura and that in B. mori and D. melanogaster. [score:1]
Cloning and Analysis of pre-miR-14 and pre-miR-2a from S. lituraTo verify the present results, homology of pre-miR-14 and pre-miR-2a were cloned from S. litura. [score:1]
Cloning and Analysis of pre-miR-14 and pre-miR-2a from S. litura. [score:1]
Pre-miR-14 and pre-miR-2a as representatives were also cloned from S. litura; both their sequences and secondary structures shared a high degree of homology with those in mo del insects, and the mature sequences of miR-14 and miR-2a in their precursors shared 100% identity to the results shown by stem-loop RT-PCR. [score:1]
The mature sli-miR-14 and sli-miR-2a in their precursors shared 100% identity to the results shown by stem-loop RT-PCR. [score:1]
PCR of sli-miR-14, sli-miR-2a and sli-bantam. [score:1]
This fragment showed 97.5% identity to the ESTs of S. frugiperda and 82 bp putative pre-sli-miR-14 was contained in it (Figure 2a). [score:1]
Amplification and Identification of sli-miR-14, sli-miR-2a and sli-bantam from S. litura. [score:1]
Three homologues of known miRNAs, miR-14, miR-2a and bantam in mo del insects, were cloned from S. litura by stem-loop RT-PCR, and named sli-miR-14, sli- miR-2a and sli-bantam. [score:1]
In order to confirm whether the PCR products are endogenous miRNAs, putative precursors of miR-14 and miR-2a as representatives were also cloned from S. litura, results showed that both their sequences and secondary structures were highly conserved with mo del insects. [score:1]
The efficiency of miR-14, miR-2a and 5S rRNA was close to the ideal value of 2 (Table 1), therefore relative quantification (RQ) of miRNA expression was calculated with 2 [−Δ ΔCt] method [28, 29]. [score:1]
To identify the availability of stem-loop RT-PCR technology for cloning conserved miRNAs from non-mo del insects, the sequences of miR-14, miR-2a and bantam in mo del insects were searched from miRBase (Table S1). [score:1]
Partial sequence of the two ESTs showed high similarities with pre-bmo-miR-14 and pre-dme-miR-14. [score:1]
Genomic DNA Isolation and the Amplification of pre-miR-14 and pre-miR-2a from S. lituraTotal genomic DNA was isolated from the adult of S. litura according to the instruction of E. Z. N. A. Insect DNA Kit (OMEGA, USA). [score:1]
To verify the present results, homology of pre-miR-14 and pre-miR-2a were cloned from S. litura. [score:1]
Moreover, both mature sequences of miR-14 and miR-2a in their precursors shared 100% identity to the results shown by stem-loop RT-PCR. [score:1]
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3
[+] score: 48
Other miRNAs from this paper: dme-mir-34, dme-mir-9c, dme-mir-989
GO term comparisons of miR-14 and miR-34 putative targets and of all the Drosophila miRNA putative targets. [score:5]
To determine whether these putative inhibitors of the piRNA pathway are part of a single biological process that antagonizes piRNA -mediated TE repression, we performed a gene ontology (GO) term enrichment analysis on the miR-14 and miR-34 target genes using GOrilla (http://cbl-gorilla. [score:5]
1005194.g007 Fig 7GO term comparisons of miR-14 and miR-34 putative targets and of all the Drosophila miRNA putative targets. [score:5]
We compared the 153 miR-14 and the 98 miR-34 putative targets using as background set the 3759 genes that are putatively targeted by all Drosophila miRNAs. [score:4]
We report here that, in Drosophila somatic ovarian tissues, two miRNAs, miR-14 and miR-34, are required for the accumulation of piRNAs that prevent the expression of transposable elements and, probably, the subsequent invasion of the germinal genome. [score:3]
org/fly_12/), we found 153 and 98 putative targets for miR-14 and miR-34, respectively. [score:3]
The presence of a comparable piRNA loss (Fig 6E) and TE de-repression (S4 Fig), in this mutant ruled out a possible off-target effect of the miR-14-SP approach. [score:3]
Conversely, the “plasma membrane component” GO term was significantly enriched in miR-14 target genes (P-value 5.2E-4) (Fig 7 and S6 Table). [score:3]
List of miR-14 putative target genes enriched in the GO terms GO0016020 and GO0030139. [score:3]
We observed similar phenotypes upon individual titration (by expression of the corresponding miR-sponge) of miR-14 and miR-34, and also in a miR-14 loss of function mutant. [score:3]
Next we wanted to identify the gene(s) that are regulated by miR-14 and miR-34 for piRNA -mediated TE repression in follicle cells. [score:2]
Moreover, like upon drosha and AGO1 knock down, the level of two piRNAs (ZAM and Tabor), quantified by RT-qPCR, was clearly decreased following miR-SP -induced miR-14 and miR-34 titration (Fig 6D). [score:2]
MiR-SP mediated titration of two miRNAs (miR-14 and miR-34) resulted in lacZ de-repression, as indicated by β-Gal staining and RT-qPCR (Fig 6A– 6B). [score:1]
miR-14 and miR-34 are specifically required for TE piRNA biogenesis and/or stability in follicle cells. [score:1]
Moreover, we report that individual titration of two miRNAs (miR-14 and miR-34) leads to a similar TE de-repression phenotype. [score:1]
Two Drosha -dependent miRNAs, miR-14 and miR-34, are therefore individually required for both TE repression and TE-derived piRNA accumulation in follicle cells. [score:1]
For instance, oogenesis did not seem to be affected when miR-sponge -mediated titration of either miR-14 or miR-34 resulted in gypsy- and ZAM-lacZ de-repression. [score:1]
We could partly confirm the results of this screen by using a miR-14 null mutant. [score:1]
miRNA screen and genetic validation of the requirement of miR-14 for TE repression. [score:1]
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4
[+] score: 46
Further examination of miR-14 expression across adult lifespan showed a relatively consistent expression regardless of age, sex, and hematophagy (Figure 5). [score:5]
Further research on the targets and function of miR-14 in mosquitoes will help determine whether it is important to mosquito longevity. [score:3]
Consistent expression of miR-14 suggests that it is likely important across all mosquito life stages from embryos to aged adults. [score:3]
miR-14 was expressed in the same stages in An. [score:3]
B) miR-14 expression in An. [score:3]
We also determined the expression profile of miR-14, which represents 25% of the miRNA sequences during our cloning experiment (Table 2). [score:3]
We provided extended expression analysis on miR-14, the miRNA that represents 25% of the sequenced miRNAs during the cloning of the 17-day old An. [score:3]
A) miR-14 expression in An. [score:3]
miR-14 expression is consistent during the adult lifespan regardless of age, sex, and blood feeding status. [score:3]
Consistent expression of miR-14 suggests that miR-14 is likely important across all mosquito life stages from embryos to aged adults. [score:3]
Intriguingly, miR-14, which is involved in the regulation of apoptosis and longevity in D. melanogaster [26], represents 25% of all the identified miRNAs. [score:2]
We showed that the miR-14 level increased slightly during embryonic development and remained relatively high through larvae, pupae and adult stages and we did not observe significant changes in adults regardless of age, sex, and blood feeding status. [score:2]
A mosquito homolog of miR-14, a regulator of longevity and apoptosis in D. melanogaster, represented 25% of all sequenced miRNA clones from 17-day old An. [score:2]
Shown here are northern blots performed using Dig-labeled miRCURY LNA probes designed for hybridization to miR-14. [score:1]
As shown in Figure 1, miR-14 displays a strong signal starting from late embryonic to adult stages. [score:1]
Nonetheless, it appears that miR-14 is important across different mosquito life stages from embryos to aged adults. [score:1]
miR-14 is observed in all life stages in D. melanogaster as well [37]. [score:1]
These results do not necessarily imply that mosquito miR-14 is important to longevity, a function of miR-14 demonstrated in D. melanogaster. [score:1]
Thus miR-14 is likely important across all mosquito life stages. [score:1]
Shown here are eight northern blots performed using Dig-labeled miRCURY LNA probes designed for hybridization to either miR-14, let-7, miR-9a, miR-210, or to one of the four novel miRNAs (miR-x1–x4). [score:1]
stephensi miR-14 displayed a relatively strong signal from late embryonic to adult stages. [score:1]
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5
[+] score: 45
One hour after HS, miR-14-5p was downregulated in all of the studied strains and was restored to its original level at 24 h, whereas miR-14-3p expression remained stable in the hsp70 [−] and yw strains; however, in the w [1118] strain, miR-14-3p was upregulated (figure 5 d, e). [score:9]
Surprisingly, although pri-mir-14 and pri-mir-308 were downregulated after HS, mature miR-14-3p and miR-308-5p were upregulated (figures  5 a and 4 b; electronic supplementary material, table S3). [score:7]
Despite the observed downregulation of pri-mir-14 and pri-mir-308, mature miRNAs (e. g. miR-14-3p and miR-308-5p) originating from these precursor molecules exhibit upregulation after HS (figure 5 a; electronic supplementary material, table S3). [score:7]
As computational miRNA target prediction shows, hsp70 transcripts may be a target for regulation by a set of abundant miRNAs; for example, miR-14 and miR-8 (data not shown). [score:6]
Because miR-14-3p regulates lipid and insulin metabolism and suppresses cell death, we speculate that its involvement in the regulation of these processes is crucial especially during the HSR but may significantly differ in different strains under normal physiological conditions [41, 42]. [score:5]
12, 371 (doi:10.1186/1471-2164-12-371) 41 Xu P, Vermooy S, Guo M, Hay B 2003 The Drosophila microRNA miR-14 suppresses cell death and is required for normal fat metabolism. [score:3]
Using qPCR, we found that HS exposure led to a decrease in the expression (two- to 2.5-fold, p ≤ 0.05) of several selected pri-miRNAs, specifically, pri-mir-14, pri-mir-6, pri-mir-286, pri-mir-311 and pri-mir-308 in the w [1118] strain (figure 5 a). [score:3]
One example involves miR-14-3p, which exhibits minimal expression levels under normal conditions but is actively accumulated upon HS treatment in the w [1118] strain (figure 5 d, e). [score:3]
After a 24 h recovery period, pri-mir-14, pri-mir-6 and pri-mir-286 gradually returned to their original levels. [score:1]
Based on this premise, we observed the HS -induced ‘arm-switching’ of miR-14. [score:1]
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6
[+] score: 27
Other miRNAs from this paper: dme-mir-5, dme-mir-6-1, dme-mir-6-2, dme-mir-6-3, dme-mir-309
Analysis of the expression levels of Hr78 by semi-quantitative RT-PCR revealed that, indeed, Hr78 mRNA stabilization depends on miR-14 dosage, with highest expression in the homozygous mutant background, intermediate expression in the heterozygous condition and lowest expression levels in the wild type (Figure 10c). [score:9]
To test this hypothesis, we studied the expression of Hr78, an unstable mRNA (Figure 10a) predicted to be targeted by miR-14 (Figure 10b) in embryos with two (wild type), one (heterozygous mutant) and no genomic copies (homozygous mutant) of miR-14 (Figure 10c). [score:5]
To establish whether modulations of miRNA level had an impact on RNA degradation patterns in vivo, we focused on miR-14, which is known to be present during early embryogenesis [75] and has multiple predicted targets within our instable RNA classes. [score:3]
Figure 10Effects of miR-14 on mRNA expression during early Drosophila embryogenesis. [score:3]
We confirmed the activity of one predicted RNA decay regulator, miR-14, in early embryos experimentally (Figure 10). [score:2]
These results are consistent with an active role of miR-14 in RNA stability control during early Drosophila development, as predicted by our study. [score:2]
miR-14 heterozygous and homozygous embryos were recovered from miR-14 Δ[1]/CyO [98] (a gift from Stephen Cohen). [score:1]
Semi-quantitative RT-PCR experiments for Hr78 were carried out on RNA samples from wild-type (+/+) and embryos heterozygous (ΔmiR-14/+) or homozygous (ΔmiR-14miR-14) for a miR-14 deletion. [score:1]
Lowering the dose of miR-14 led to stabilization of Hr78 mRNAs in a dose -dependent manner. [score:1]
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7
[+] score: 22
Other miRNAs from this paper: dme-mir-278
miR-278 down-regulates dilps expressed in the IPCs while miR-14 positively regulates dilp3 and dilp5 expression. [score:9]
Drosophila miR-14 regulates insulin production and metabolism through its target, sugarbabe. [score:4]
Interestingly, the hyperlipidemic defect seen in miR-14 mutants was rescued by overexpressing dilp3 implying that miR-14 regulates lipid metabolism through modulation of dilp3 and also outlines a role for dilp3 in this regard (Varghese et al., 2010). [score:4]
One such miRNA, miR-14 expressed in Drosophila IPCs systemically regulates fat levels. [score:4]
Using a reverse genetic approach, Varghese et al. detected reduced dilp3 and dilp5 mRNA levels in miR-14 mutant flies, which accompanied increased triglyceride levels (Varghese et al., 2010). [score:1]
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8
[+] score: 16
Overexpression of non-mammalian (drosophila, C. elegans) and endogenous mouse miRNAsIt was previously reported that miRNAs derived from C. elegans or other lower species are difficult to express efficiently in mammalian cells in vitro 1. We therefore sought to compare the expression efficiency of endogenous mouse miRNAs (mir-107, mir-122, mir-675) and exogenous miRNAs from two non-mammalian species, namely drosophila (mir-14 and mir-276), and C. elegans (mir-77, mir-230). [score:7]
It was previously reported that miRNAs derived from C. elegans or other lower species are difficult to express efficiently in mammalian cells in vitro 1. We therefore sought to compare the expression efficiency of endogenous mouse miRNAs (mir-107, mir-122, mir-675) and exogenous miRNAs from two non-mammalian species, namely drosophila (mir-14 and mir-276), and C. elegans (mir-77, mir-230). [score:5]
We were able to recapitulate the lower expression level of C. elegans (miR-77-3p and miR-230-3p) miRNA and, to a lesser extent, of drosophila miRNA (miR-14-3p and miR-276a-3p), using reverse pri-mir or pre-mir strategy. [score:3]
This included the drosophila miR-14 (n = 5–9) and miR-276a (n = 5–15), the C. elegans miR-77 (n = 5–12) and miR-230 (n = 6–10), and mouse miR-107-3p (n = 6–15), miR-122-5p (n = 3–12), and miR-675-3p (n = 6–11). [score:1]
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9
[+] score: 10
Therefore, feeding of the DCPTN-PT containing culture media did not depress the regulatory microRNAs (miR-2, miR-13 and miR-14) that control the translation of three pro-apoptotic host genes (miR-2 and miR-13 targets rpr, grim and miR-14 targets Drice), suggesting that DCPTN-PT compound is not an inhibitor of all proapoptotic genes but very specific to oncomiR bantam. [score:10]
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10
[+] score: 8
In contrast, mir-14-3p was greater expressed in the embryo than in the egg. [score:3]
Some other maternal microRNAs have roles unrelated with embryonic development, such as mir-14, which regulates insulin production (Xu et al. 2003); mir-279, involved in the circadian clock (Luo and Sehgal 2012); or mir-8, associated to abdominal pigmentation (Kennell et al. 2012), to name but a few cases. [score:3]
Although the microRNA level varies substantially across biological replicates, the presence of seven of the maternal microRNAs here described is validated (bantam-3p, mir-311-3p, mir-92b-3p, mir-184-3p, mir-14-3p, mir-995-3p, and mir-9c-5p), although the levels of the latter two were relatively low. [score:1]
Another maternal microRNA gene, mir-14, seems to be involved in transcriptional silencing of transposable elements in the germline (Mugat et al. 2015). [score:1]
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11
[+] score: 7
However, the expression levels of miR-1-3p and miR-100-5p were significantly increased after treatment with 20E, whereas those of bantam-3p, miR-125-5p, miR-14-3p, miR-276-3p, miR-34-5p and let-7-5p showed a tendency to increase with respect to controls, although differences were not statistically significant. [score:3]
miR-14-3p has been described targeting the ecdysone receptor (EcR) in D. melanogaster[29], and miR-34-5p is inducible by the JH analogue methoprene in Drosophila S2 cells [30]. [score:3]
Finally, we included two additional miRNAs (miR-14-3p and miR-34-5p) because of their potential interest in the context of moulting and metamorphosis. [score:1]
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12
[+] score: 6
The micro -RNA miR-14 induces autophagy of the salivary glands during early pupal life by targeting IP3K2 (Nelson et al. 2014). [score:3]
These findings suggest the intriguing possibility that IP3K2 and IP3R regulate autophagy in the developing wing, perhaps by interactions with the Atg6/ miR-14 module. [score:2]
This same study suggested that Atg6, an autophagy-inducing gene that encodes a component of the Vps34 phosphatidylinositol 3-kinase (PI3K) complex III, acts in the same pathway as miR-14. [score:1]
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13
[+] score: 6
bmo-miR-1, bmo-let-7a, bmo-miR-8, bmo-miR-14, bmo-miR-276a, bmo-miR-279 were strongly expressed in all developmental stages (larva, pupa and moth). [score:4]
We designated these sequences bmo-miR-13a*, bmo-miR-14, bmo-miR-46, bmo-miR-46*, bmo-miR-71, bmo-miR-277 and bmo-bantam. [score:1]
Using methylated DNA probes, signal strength was significantly improved in Northern blots for bmo-miR-14 and bmo-miR-8*, which could not be detected using non-methylated DNA probes [see Figure S2 of Additional file 1]. [score:1]
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14
[+] score: 6
In C. elegans, the miRNAs lin-4 (Lee et al. 1993; Olsen and Ambros 1999) and let-7 (Reinhart et al. 2000) regulate developmental timing, whereas the Drosophila miRNAs bantam and miR-14 control cell survival by repressing translation of proapoptotic genes (Brennecke et al. 2003; Xu et al. 2003). [score:5]
gov/LocusLink/) ID numbers for the genes discussed in this paper are alg-1 (181504), alg-2 (173468), bantam (117376), let-7 (266954), lin-4 (266860), lin-41 (172760), miR-14 (170868), and rde-4 (176438). [score:1]
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15
[+] score: 4
Drosophila miR-14 regulates insulin production and metabolism through its target, sugarbabe. [score:4]
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16
[+] score: 4
It was reported that Drosophila microRNA mir-14 inhibits reaper -dependent cell death and is required for lipid metabolism (Xu et al., 2003). [score:3]
Depletion of mir-14 results in reduced lifespan and lowered stress tolerance and is accompanied with increased levels of triacylglycerol and diacylglycerol and the above phenotypes are reverted upon increasing mir-14 copy number in Drosophila. [score:1]
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17
[+] score: 4
The expression of the non-related microRNA miR-14 driven by L4 had no effect on nicotine HRT or cuticle phenotype (Fig 5B). [score:3]
UAS-iRNA esg (Bloomington Stock # 34063), snail [18] (Bloomington Stock # 2311), Δ40 line (deletion of cluster miR-310 [c]), UAS-DsRed-53-miR-310-313 were kindly provided by Pejmun Haghighi Lab (McGill University), individual UAS-LUC-miR-311, UAS-LUC-miR-14 and all other lines used in this work are available at the Bloomington Drosophila Stock Center (NIH P40OD018537). [score:1]
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18
[+] score: 3
However, there are numerous examples of microRNAs that are present in RISC more than expected from their expression levels (miR-14-3p, miR-317-3p, 275-3p) or less than expected (miR-13a-3p, miR-317-5p, miR-190-3p). [score:3]
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19
[+] score: 2
Other potential losses in chelicerates include mir-14 and mir-3477, which was only present in P. tepidariorum and S. mimosarum. [score:1]
The conserved families of mir-14, mir-3477, and mir-3791 were present in S. mimosarum, but absent in A. genticulata and C. sculpturatus (fig. 2). [score:1]
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20
[+] score: 2
miR-14 mutants do not impact on IOB apoptosis ([36] and our unpublished observations). [score:1]
miR-14 has also been reported to be anti-apoptotic [35]. [score:1]
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21
[+] score: 2
Other miRNAs from this paper: dme-mir-2a-1, dme-mir-2a-2, mmu-mir-184, dme-mir-184, hsa-mir-184
Experiments using miR-14 and miR-184 gave similar results (Additional file 5, Figure S5). [score:1]
Strikingly, the order of preference for nt 1 was not the same across the three tested miRNA: miR-2a preferred U > A > C (Figure 3), miR-14 preferred U ~ C > A and miR-184 preferred U ~ A > C (Additional file 6, Figure S6). [score:1]
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22
[+] score: 1
37), thus leaving 4% as conclusive false positives (miR-14, miR-79, miR-307 and miR-975; dark blue in Fig. 2b and ). [score:1]
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[+] score: 1
Other miRNAs from this paper: dme-mir-8
In miR-14 mutant flies, sug was found to control dIlp mRNA levels, suggesting that it may act as a transcription factor to these genes [69]. [score:1]
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[+] score: 1
Other miRNAs from this paper: dme-mir-8
Recently, several novel components of the insulin pathway were identified, including miRNAs (miR-8 and miR-14) and secreted proteins (Upd and SDR) [13]– [16]. [score:1]
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