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24 publications mentioning mmu-mir-291a

Open access articles that are associated with the species Mus musculus and mention the gene name mir-291a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 61
Considering the targets for those miRNAs either highly expressed in WT ES cells or for whom there is a clear seed sequence enrichment amongst those genes up-regulated following Dgcr8 depletion (Table 2), Cdkn1a (miR-291a-3p, miR-302a and miR-106a) and E2f2 (miR-106a, miR-302a and miR-21) both appear to be potentially targeted by three miRNAs, while Skp2 (miR-21 and miR-302a) and Cyclin D1 (miR-106a and miR-302a) may be targeted by two each. [score:12]
In addition, the number of targets generated above expected, varied between 242 target transcripts for miR-302a to 20 target transcripts for miR-291a-3p (Table S2). [score:7]
Although the targets of miR-291a-3p are not enriched in this pathway, this is not surprising given the relatively small size of the miR-291a-3p target list. [score:5]
Briefly, 1500 ng of biotinylated cRNA was hybridised to Illumina expression BeadChips (Mouse-6 v1.1 for mmu-miR-291-3p and mmu-miR-25 mimics and cell line expression profiles, and Mouse-6 v2 for mmu-miR-302, mmu-miR-292-5p, mmu-miR-106a, mmu-miR-21 and mmu-miR-298 mimics. [score:5]
Cdkn1a, a key regulator of the G1/S phase transition, also appears in our miR-302a and miR-291a-3p target lists. [score:4]
The functional redundancy expected between the targets of miR-291a-3p and miR-302a, which share a seed sequence, is clearly evident. [score:3]
This implies a degree of cross-regulation and redundancy not only between miRNAs with shared seed sequences (miR-291a-3p and miR-302a) but also both amongst miRNAs with no shared seed identity and through the regulation of multiple components of a functional complex. [score:3]
These two miRNAs share 86% of the miR-291a-3p target transcripts. [score:3]
A: Sylamer plots comparing the expression profiles of Dgcr8 [gt1/tm1] cells transfected with a miRNA mimic (miR-25, miR-291a-3p, miR-292-5p or miR-298) and a cel-miR-239b control miRNA. [score:3]
Intriguingly, miR-106a also shares a shifted seed sequence (7mer(3)) with both miR-291a-3p and miR-302a (7mer(2)), which may also suggest that they maintain functionally redundant roles through interaction with the same target sites. [score:3]
For a full description see Figure 3. B: GSEA enrichment plots [29] judging the enrichment of the transcripts within the miRNA target lists for miR-25, miR-291a-3p, miR-292-5p or miR-298 within regions of a list of transcripts ordered according to log fold change following the depletion of Dgcr8 in homozygous mutant cell lines. [score:3]
In addition miR-298, miR-302a, miR-21 and miR-291a-3p appear to target genes in these pathways to varying degrees. [score:3]
Mouse ES cells bearing mutations in the miRNA processing pathway had an extended G1/S phase phenotype that was rescued by transfection of members of miRNA families that include miR-106a, miR-302a and miR-291a-3p [22]. [score:2]
Of these, miR-302a, miR-21 and miR-291a-3p, or their human homologues, have all been associated with cancer [33], [34]. [score:1]
miRIDIAN Negative Control #2 (Dharmacon CN-002000-01-05) miRIDIAN mmu-miR-291-3p mimic (Dharmacon C-310470-01-0005) miRIDIAN mmu-miR-25 mimic (Dharmacon C-310564-01-0005) miRIDIAN mmu-miR-302 mimic (Dharmacon C-310483-05-0005) miRIDIAN mmu-miR-292-5p mimic (Dharmacon C-310471-03-0005) miRIDIAN mmu-miR-106a mimic (Dharmacon C-310488-07-0005) miRIDIAN mmu-miR-21 mimic (Dharmacon C-310515-05-0005) miRIDIAN mmu-miR-298 mimic (Dharmacon C-310479-07-0005) Trizol purified RNA was cleaned up with an RNeasy MiniElute Cleanup Kit (Qiagen). [score:1]
miRIDIAN Negative Control #2 (Dharmacon CN-002000-01-05) miRIDIAN mmu-miR-291-3p mimic (Dharmacon C-310470-01-0005) miRIDIAN mmu-miR-25 mimic (Dharmacon C-310564-01-0005) miRIDIAN mmu-miR-302 mimic (Dharmacon C-310483-05-0005) miRIDIAN mmu-miR-292-5p mimic (Dharmacon C-310471-03-0005) miRIDIAN mmu-miR-106a mimic (Dharmacon C-310488-07-0005) miRIDIAN mmu-miR-21 mimic (Dharmacon C-310515-05-0005) miRIDIAN mmu-miR-298 mimic (Dharmacon C-310479-07-0005) Trizol purified RNA was cleaned up with an RNeasy MiniElute Cleanup Kit (Qiagen). [score:1]
A set of miRNAs was transfected including miR-25, miR-291a-3p, miR-292-5p, miR-106a, miR-21, miR-302a and miR-298. [score:1]
Initially, miR-291a-3p and miR-25 were transfected into the Dgcr8 [gt1/tm1] cells. [score:1]
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2
[+] score: 45
Other miRNAs from this paper: mmu-mir-290a, mmu-mir-290b
b The expression of Gas5 in miR-291a KD mESCs c Overexpression of miR-291a restores the expression of Gas5 in Dicer knockdown mESCs. [score:8]
Conversely, forced expression of cMyc in Dicer or miR-291a KD mESCs could counteract the downregulation of Gas5 (Fig. 5e). [score:6]
Furthermore, overexpression of miR-291a in Dicer knockdown mESCs rescued the expression of Gas5 (Fig. 5c). [score:6]
e Enforced expression of cMyc rescues the expression of Gas5 in Dicer and miR-291a KD mESCs. [score:5]
Taken together, these data suggest a possible Dicer-miR291a regulatory pathway for the endogenous Gas5 expression in mESCs. [score:4]
From our experiments, cMyc KD mESCs showed decreased Gas5 expression, similar to what we observed in Dicer KD and miR-291a KD mESCs (Fig. 5d). [score:3]
Gas5 is regulated by the Dicer-miR291a–cMyc pathway in ESCs. [score:2]
MiR-291a mimics and inhibitors was purchased from GenePharma (Shanghai, China). [score:2]
In addition, Gas5 was regulated by the Dicer-miR291a–cMyc axis and was involved in the DNA demethylation process in mESCs. [score:2]
Overall, these results suggest that Gas5 is regulated by the Dicer-miR-291a–cMyc axis in mESCs. [score:2]
The regulatory axis of Dicer-miR291a–cMyc-Gas5 and the relationship between Gas5 and Tet/5hmC in mESCs was examined by qRT-PCR,, and. [score:2]
Fig. 5Gas5 is regulated by the Dicer-miR291a–cMyc pathway in ESCs. [score:2]
Indeed, Gas5 was repressed in miR-291a (the most abundant miRNA of the miR-290 cluster in mESCs) KD mESCs (Fig. 5b). [score:1]
[1 to 20 of 13 sentences]
3
[+] score: 26
Therefore, we selected five DEMs, three upregulated (mmu-miR-192-5p, mmu-miR-291a-3p and mmu-miR-320-3p), and two downregulated (mmu-miR-139-5p and mmu-miR-378a-3p), construct miRNA-mRNA network to explore the regulatory mechanisms of miRNAs. [score:8]
In this study, we selected five DEMs, three upregulated (mmu-miR-192-5p, mmu-miR-291a-3p and mmu-miR-320-3p), and two downregulated (mmu-miR-139-5p and mmu-miR-378a-3p), to construct the miRNA-mRNA network (Figure 6). [score:7]
Red (mmu-miR-192-5p, mmu-miR-291a-3p and mmu-miR-320-3p) and Green (mmu-miR-139-5p and mmu-miR-378a-3p) are square nodes represent up-regulated and down-regulated miRNAs, respectively. [score:7]
The miRNA-mRNA network revealed that the potential target genes of Crtc2 (miR-291a-3p), Pik3 (miR-320-3p), and Pik3ca (miR-320-3p) are associated with PI3K-Akt and FoxO signaling pathway. [score:3]
According to the network we can intuitive to see that, mmu-miR-192-5p was associated with 18 mRNAs, mmu-miR-291a-3p was associated with 66 mRNAs and mmu-miR-320-3p was associated with 44 mRNAs. [score:1]
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4
[+] score: 25
For example, Hlf was targeted by mmu-miR-18b-5p, mmu-miR-429-3p and mmu-miR-291a-3p; Cnot6 and Zfp91 were targeted by mmu-miR-206-3p and mmu-miR-291a-3p; Rbfox2 was targeted by mmu-miR-429-3p and mmu-miR-449c-5p; Aebp2 was targeted by mmu-miR-125a-3p, mmu-miR-223-3p and mmu-miR-496-3p; Nfya was targeted by mmu-miR-199a-5p, mmu-miR-216b-5p and mmu-miR-671-5p; Nucks1 was targeted by mmu-miR-142-3p, mmu-miR-146a-5p and mmu-miR-223-3p; Notch2 was targeted by mmu-miR-146a-5p and indirectly via Ascl1 by mmu-miR-1903. [score:16]
Seven of the up-regulated miRNAs (miR-465b, miR-466f, miR-669o, miR-18b, miR-291a, miR-696, miR-101a) were also much more (≥ 5 fold) expressed in the retina compared to the sclera suggesting that they might be involved in the regulation of retina-specific processes. [score:6]
MP1, MP2, MP3, MP4, MP5, MP6 and MP7 had a common core comprised of mmu-miR-1-3p, mmu-miR-145-5p, mmu-miR-18a-5p, mmu-miR-199a-5p, mmu-miR-200b-3p, mmu-miR-223-3p, mmu-miR-291a-3p, mmu-miR-34a-5p and their target mRNAs. [score:3]
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5
[+] score: 17
Among these four miRNAs, only miR-291a-3p was upregulated in the lead-treatment alone group, in which the expression of Uc. [score:6]
Analysis of miRSystem revealed among the eight miRNAs, four miRNAs (miR-291a-3p, miR-295-3p, miR-302b-3p and miR-302d-3p) have apoptosis -associated target genes (PI3K, NIK and Cn) or a DNA-damage repair -associated target gene (RAD23B). [score:5]
173 likely inhibits apoptosis through the mediation of miR-291a-3p, which needs further studies to verify. [score:3]
173 potentially regulated apoptosis through the mediation of miR-291a-3p. [score:2]
173 (miR-186-5p, miR-208a-5p, miR-291a-3p, miR-294-3p, miR-295-3p, miR-302a-3p, miR-302b-3p, miR-302c-3p and miR-302d-3p). [score:1]
[1 to 20 of 5 sentences]
6
[+] score: 16
ch/ElMMo2/) of predicted target transcripts revealed a consensus set of regulated cellular functions for miR-291a-3p, miR-292-3p, miR-294 and miR-295, but not for miR-293 (Dataset S2 for the EIMMo target prediction software and Dataset S3 for the Pictar target prediction software). [score:8]
We also used a miR-291a-3p duplex as a representative of the miR-290 family, of which several members are also predicted to target Arid4b owing to seed identity with miR-302 (Figure 4D, Dataset S2 and Dataset S3). [score:3]
In agreement with these previous studies, separate time-course analyses of each member revealed that only 4 miRNAs, which share the same AAAGUGC 5′ seed sequence (miR-291a-3p, miR-292-3p, miR-294, miR-295, Figure 2A, blue), likely contribute significantly to the global trend of miR-290 cluster expression (reduced throughout differentiation, PAM class A; Figure 2A, grey). [score:3]
24 h post-transfection, a similar decrease in Renilla luciferase reporter gene activity was observed with both miR-302d and miR-291a-3p treatments, but not upon transfection of the control siRNA duplex (Figure 4D), indicating a sequence-specific effect. [score:1]
HEK-293 cells were transiently transfected with psiCHECK-Arid4b together with miR-302d, miR-291-3p, or a control siRNA, using lipofectamine 2000 (Invitrogen). [score:1]
[1 to 20 of 5 sentences]
7
[+] score: 13
We chose to profile the ubiquitously expressed miR-16, five ESC-specific miRNAs (miR-290, miR-291-3p, miR-292-3p, miR-294, and miR-295) [23], [24], and two miRNAs that are upregulated in ESCs undergoing differentiation (miR-21 and miR-22) [23], [24]. [score:6]
RNU6b is significantly less abundant than all miRNAs tested except for miR-22, miR-290, and miR-291. [score:1]
The miRNAs tested include miR-16 (lane 1), miR-21 (lane 2), miR-22 (lane 3), miR-290 (lane 4), miR-291-3p (lane 5), miR-292-3p (lane 6), miR-294 (lane 7), miR-295 (lane 8), and the small nuclear RNA, RNU6b (lane 9). [score:1]
Thus, based on the 95 [th] percentile confidence interval, it appears that RNU6b is significantly less abundant than several of the miRNAs tested except miR-22, miR-290, and miR-291. [score:1]
The difference in Ct values between the negative control (MEFs alone) and each experimental group (miR-290, miR-291-3p, miR-292-3p, miR-294, miR-295, miR-16, and RNU6b) is shown. [score:1]
The relative abundance of all tested miRNAs overlaps except for that of miR-295, which is significantly more abundant than miR-290 and miR-291 (Figure 5B). [score:1]
The abundance of several miRNAs (miR-290, miR-291-3p, miR-292-3p, miR-294, and miR-295) increased in MEFs as early as 1 hour after incubation, suggesting transfer. [score:1]
The majority of miRNAs tested do not differ significantly from one another except for miR-295, which is significantly more abundant than miR-290 and miR-291. [score:1]
[1 to 20 of 8 sentences]
8
[+] score: 10
Other miRNAs from this paper: mmu-mir-290a, mmu-mir-291b, mmu-mir-219b, mmu-mir-290b
In our previously study, we identified AMPK as a primary target of miR-291, and revealed that miR-291 inhibitors ameliorated hepatic TG accumulation, reduced FAS and SREBP-1 activity and this was attributed to the targeting of miR-291b-3p of AMPK 24. [score:7]
Moreover, to assess whether the effect of miR-291 on insulin resistance is dependent on AMPK, compound c (Sigma), an AMPK inhibitor, was intravenously injected at a dose of 2 mg/kg for 3 days before sacrifice. [score:3]
[1 to 20 of 2 sentences]
9
[+] score: 9
Thus, similarly to its interaction with the mouse miR-291-3p0, miR-294-3p0 and/or miR-295-3p0, the 2-7C-S target appears to interact via G:U wobble base pairing with miR-372-3p0 and/or miR-373-3p0 which also have a 2-7U seed. [score:3]
In this case pre-miR-295 and pre-miR-372 would express the same single 2-7U 7mer seed, which is shared with pre-miR-291a, pre-miR-294 and pre-miR373 and, importantly, is represented in all homologous miR-290–295/miR-371–373 loci. [score:3]
However, discrepancies between the various datasets, make the unambiguous assignment of active mature miRNAs to each pre-miRNA hairpin difficult (Figure 2, total RNA datasets for pre-miR-291a, pre-miR-293, pre-miR-294 and total RNA versus HITS-CLIP data for pre-miR-290). [score:1]
The 2-7C-S miRNA binding site might be recognized by 3p0 miRNAs with 2-7U seeds (miR-291a-3p0, miR-294-3p0 and miR-295-3p0, Figure 4A) via a G:U wobble at position 8 of the miRNA seed. [score:1]
Therefore, pre-miR-291a, pre-miR-291b and pre-miR294 are likely co-orthologs of pre-miR-372 and pre-miR-295 is an ortholog of the promoter distal pre-miR-373. [score:1]
[1 to 20 of 5 sentences]
10
[+] score: 9
Expression levels of these miRNAs were maintained in both male and female germ cells at E12.5 and E13.5, except miR-291-5p and miR-292-3p both of which were slightly down-regulated in E13.5 female PGCs (Figure 1A). [score:6]
MiRNAs belonging to miR-17-92 and miR-290-295 clusters were still highly expressed in neonatal spermatogonia and at almost the same levels as those in E13.5 PGCs (Figure 4E), although miR-290 and miR-291-3p were not detectable. [score:3]
[1 to 20 of 2 sentences]
11
[+] score: 8
The other miRNAs of the miR-290-295 cluster (miR-290-5p, miR-291a-5p, miR-291b-5p, miR-292-5p, miR-293, miR-293 [*], miR-294 [*], and miR-295 [*]) differing in their seed sequences, are still highly expressed in ESCs with the exception of the hardly detectable [22] minor forms of miR-293, miR-294, and miR-295 (miR-293 [*], miR-294 [*], and miR-295 [*]). [score:3]
More interestingly, Ash1l is a target of miR-291, which was validated by using reporter assays [65]. [score:2]
Within the miR-290-295 cluster, the seed sequences of ‘AAGUGC’ hexamer are found in miR-290-3p, miR-291a-3p, miR-291b-3p, miR-292-3p, miR-294, and miR-295. [score:1]
miR-290-291a unit replication formed miR-292-291b, and then miR-290, miR-291a and miR-292 (as the same unit) replication resulted in the formation of miR-293, miR-294 and miR-295 ESC and iPSC self-renewals need to eliminate differentiation signal and obtain the pluripotency signal, in addition, the differentiation process trigger the closure of pluripotency procedure and the induction of lineage specification. [score:1]
The miR-290-291 unit replication forms miR-292-291b, and then the miR-290, miR-291a and miR-292 (as the same unit) replication results in the formation of miR-293, miR-294 and miR-295, finally forming the present miR-290-295 cluster [21] (Fig.   1). [score:1]
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12
[+] score: 8
qPCR analysis of miR291-5p, miR290-3p, and miR292-3p expression levels in RNA purified from primary wild-type pro-B (B220+, CD43+, IgM−) or pre-B (B220+, CD43−, IgM−) cells. [score:3]
However, aside from miR291-5p, we could not detect their expression in sorted pre-B cells of wild-type mice. [score:3]
A third member of the miR290 polycistronic cluster with a similar seed sequence as miR290-5p/292-5p, miR291-5p, is also induced; however other members of the miR290 cluster with an alternate seed sequence did not increase upon STI571 treatment (Figure S1). [score:1]
miR290-5p/292-5p share the seed sequence CUCAAA similar to miR291-5p (100049715, 100124471), AUCAAA. [score:1]
[1 to 20 of 4 sentences]
13
[+] score: 7
Of these, 12 (mir-9, mir-200c, mir-708, mir-377, mir-26b, mir-296, mir-369, mir-32, mir-1965, mir-1190, mir-135b and mir-201) were differentially up-regulated and five (mir-291a, mir-190b, mir-297c, mir-713 and mir-470) were differentially down-regulated. [score:7]
[1 to 20 of 1 sentences]
14
[+] score: 5
For example, stem cell specific miRNAs of the miR-290 family (miR-291–295, [24]) were detected to be about 25-fold overexpressed in mouse embryonic stem cells, while brain-specific miR-124 and miR-9 were about 14-fold overexpressed in mouse brain (Supplementary Table  2). [score:5]
[1 to 20 of 1 sentences]
15
[+] score: 5
Members of the miR-290-295 cluster (miR-290, miR-291a, miR-292, miR-291b, miR-293, miR-294 and miR-295-1) were the most abundant among known miRNAs that were upregulated in rat PSCs. [score:4]
Some miRNAs, such as miR-291, miR-294 and miR-295, can enhance reprogramming that is induced by OSK factors [22]. [score:1]
[1 to 20 of 2 sentences]
16
[+] score: 4
By contrast, some miRNAs that are significantly up-regulated were also observed, including miR-290 cluster members, miR-291a and miR-291b, miR-129-1-3p, miR-129-2-3p, miR-23a-3p, miR-434-3p, miR-145-5p, and miR-203-3p. [score:4]
[1 to 20 of 1 sentences]
17
[+] score: 4
These miRNAs, which include miR-291-3p, miR-294 and miR-295, are thus named ES cell-specific cell cycle -regulating (ESCC) miRNAs based on their ability to regulate G1-S transition [39]. [score:3]
Although miR-290 itself was not known to promote reprogramming, several members of the miR-290 family, miR-291-3p, miR-294 and miR-295, enhance reprogramming of MEFs in the absence of c-Myc [41]. [score:1]
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18
[+] score: 4
In this report, some miRNAs putatively targeting the COUP-TFII mRNA were conversely regulated by androgens on GD 17.0: miR-291a-3p, miR-467b and miR-467d, and miR467h. [score:4]
[1 to 20 of 1 sentences]
19
[+] score: 3
In contrast, control miRNAs derived from the mir-291 cluster were strongly expressed in the RNA derived from ES cells, as expected. [score:3]
[1 to 20 of 1 sentences]
20
[+] score: 3
A closer look at those 5 miRNA revealed that the expression of 4 miRNA including mmu-miR-291a-5p, mmu-miR-291b-5p, mmu-miR-664-3p, and mmu-miR-1306-3p increased in tumor tissues following cigarette smoke exposure, yet they decreased in the parenchyma of exposed mice, whereas mmu-miR-378-3p displayed the same behavior in both tissues following MS exposure. [score:3]
[1 to 20 of 1 sentences]
21
[+] score: 3
On chromosome 2, five miRNAs (miR-26a, mir-291a, miR-423, miR-671 and miR-23b) were mapped between 28–51 Mb (Fig.   2). [score:1]
Examples are given by miR-671-5p (P = 1.224713e-12), miR-26a (P = 2.654271e-19) and miR-291a-3p (P = 3.530522e-02) (Additional file 1: Table S3). [score:1]
We found that 4/38 (10.53 %) miRNAs (miR-291a-3p, miR-341, miR-449b and miR-681) exhibits in dels in CAST/EiJ strain on chromosome 7 (3.2 Mb), 12 (69 and 109 Mb) and 13 (113 Mb), which may suggest false positive associations for those loci (Table  1). [score:1]
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22
[+] score: 2
Endogenous miR-291a-5p levels in Ago2 [flox/ flox]; Dicer1 [flox/ flox] ES cells are included as a comparison for transfected levels of miR-291a-5p. [score:1]
Only miR-291-3p, miR-294 and miR-295 can promote the G1-S transition of the cell cycle and the induction of pluripotency [13], [14]. [score:1]
[1 to 20 of 2 sentences]
23
[+] score: 1
miR-291a-3p, miR-291a-5p, miR-292-3p, miR-290-5p and miR-293 belong to the miR-290-295 cluster [27]. [score:1]
[1 to 20 of 1 sentences]
24
[+] score: 1
Within the mir-290-295 cluster, the ‘AAGUGC’ seed is found in miR-290-3p, miR-291a-3p, miR-291b-3p, miR-292-3p, miR-294, and miR-295. [score:1]
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