sort by

10 publications mentioning mmu-mir-293

Open access articles that are associated with the species Mus musculus and mention the gene name mir-293. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 42
0108519.g005 Figure 5(A) Predicted secondary structures of the duplexes formed between miR-292-3p+1 and miR-371-3p+1 with the 3-7CG-B target and of miR-293-3p+2 and miR-371-3p+2 with the 4-7CGC-B target. [score:5]
Importantly, the inefficient silencing of the 4-7CG-B reporter demonstrates that 6mer seed (positions 2–7) pairing of any putative 3p+2 isoforms does not contribute significantly to the silencing of 7mer target 3-7CG-B. Rescue experiments in miR-290–295 knockout ES cells confirmed that silencing of the 3-7CG-B reporter depends on pre-miR-292 and silencing of the 4-7CGC-B reporter depends on pre-miR-293 (Figure 5C, D). [score:4]
The incorporation of 3-7CG-S, 4-7CGC-S and 3-7UA-S seed only target sites, designed to interact with miR-292-3p+1, miR-293-3p+2 and miR-295-3p+1 respectively resulted in either no silencing or very inefficient silencing of the reporters (Figure 4E). [score:3]
The pre-miRNA relationships deduced from the seed expression data are also consistent with the clustering of pre-miR-371, pre-miR-290, pre-miR-292 and pre-miR-293 in a separate branch of the multiple sequence alignment UPMGA tree (Figure 1A). [score:3]
Mo dels of the miR-290–295/miR-371–373 target interaction networks should incorporate the pre-miR-371/pre-miR-290/pre-miR-292/pre-miR-293 seeds. [score:3]
Furthermore, the lack of high confidence targets for the pre-miR-290/pre-miR-292/pre-miR-293 seeds in HITS-CLIP data from mouse ES cells suggests that the corresponding miRNAs might be physiologically relevant in other biological contexts such as the extraembryonic lineages and/or TS and XEN cells. [score:3]
Thus, silencing of the bulge reporters proves that active miR-292-3p+1 and miR-293-3p+2 isoforms are expressed in mouse ES cells. [score:3]
If this hypothesis is correct, then processing of the corresponding mouse pre-miR-290, pre-miR-292 and pre-miR-293 might also be differentially regulated. [score:2]
Nevertheless, we note that the 3-7UA-S seed only reporter, which corresponds to the putative miR-295-3p+1 isoform, had the same activity in wild type and miR-290-295 knockout ES cells, whereas silencing of the 3-7CG-S and 4-7CGC-S reporters, which correspond to the active miR-292-3p+1 and miR-293-3p+2 isomiRs was consistently lower in the wild type ES cells (Figure 4E). [score:2]
Given the involvement of the 2-7U seed in cell proliferation and survival, it is likely that the phenotypes caused by the loss of the 2-7U seed miRNAs in the miR-290-295 knockout mouse mask any specific phenotypes due to the loss of function of pre-miR-290, pre-miR-292 and pre-miR-293 [20]. [score:2]
In the mouse, their seeds are represented by miR-290-5p0/miR-292-5p0, miR-292-3p+1 and miR-293-3p+2 respectively (Figure 1A, Table 1). [score:1]
Reads that originate from the mouse pre-miR-290 and the human pre-miR-371 (the most upstream hairpins in the clusters) map predominantly to the 5′- strand of the hairpin stem, suggesting that miR-290-5p and miR-371-5p and not the corresponding 3p- isomiRs are the active mature miRNA species and most sequencing data imply that miR-293-3p+2 is the sole isomiR processed from pre-miR-293 (Figure 2, pre-miR-293). [score:1]
The miR-292-3p+1/miR-371-3p+1 bulge reporter 3-7CG-B and its corresponding position 2 mismatch control 4-7CG-B (C, E) or the miR-293-3p+2/miR-371-3p+2 bulge reporter 4-7CGC-B and its position 2 mismatch control 5-7CGC-B (D, F) were co -transfected as in Figure 3C, E. Our results demonstrate that despite their different pre-miRNA organization the seed repertoires of miR-290–295 in the mouse and miR-371–373 in human are very similar if not identical (Figure 1A, Table 1). [score:1]
The miR-292-3p+1/miR-371-3p+1 bulge reporter 3-7CG-B and its corresponding position 2 mismatch control 4-7CG-B (C, E) or the miR-293-3p+2/miR-371-3p+2 bulge reporter 4-7CGC-B and its position 2 mismatch control 5-7CGC-B (D, F) were co -transfected as in Figure 3C, E. We used the 3-7CG-B and 4-7CGC-B reporters described above to test if the human pre-miR-371 produces 3p+1 and 3p+2 isoforms “seed equivalent” to the mouse miR-292-3p+1 and miR-293-3p+2. [score:1]
The miR-292-3p+1/miR-371-3p+1 bulge reporter 3-7CG-B and its corresponding position 2 mismatch control 4-7CG-B (C, E) or the miR-293-3p+2/miR-371-3p+2 bulge reporter 4-7CGC-B and its position 2 mismatch control 5-7CGC-B (D, F) were co -transfected as in Figure 3C, E. We used the 3-7CG-B and 4-7CGC-B reporters described above to test if the human pre-miR-371 produces 3p+1 and 3p+2 isoforms “seed equivalent” to the mouse miR-292-3p+1 and miR-293-3p+2. [score:1]
pre-miR-290, pre-miR-292 and pre-miR293 are co-orthologs of pre-miR-371: evolution of miR-290-295 from a three-hairpin ancestor. [score:1]
This dinucleotide is only present in the promoter-proximal pre-miRNAs of the miR-290–295/miR-371–373 cluster family (pre-miR-290 and pre-miR-371 in human) and the additional paralogs in the mouse miR-290–295 (pre-miR-292 and pre-miR-293). [score:1]
Because the sequences of pre-miR-371 on the one hand and pre-miR-292/pre-miR293 on the other vary, the predicted secondary structures of the complexes formed between 3-7CG-B and 4-7CGC-B and their cognate human and mouse miRNAs differ (Figure 5A). [score:1]
However, discrepancies between the various datasets, make the unambiguous assignment of active mature miRNAs to each pre-miRNA hairpin difficult (Figure 2, total RNA datasets for pre-miR-291a, pre-miR-293, pre-miR-294 and total RNA versus HITS-CLIP data for pre-miR-290). [score:1]
Conservation of the miR-292-3p+1/miR-371-3p+1 and miR-293-3p+2/miR-371-3p+2 seeds requires that the dinucleotide sequence CG is present at positions 3p+7 and 3p+8 of the pre-miRNA multiple sequence alignment (Figure 1A). [score:1]
Our data strongly suggest that the mouse pre-miR-290, pre-miR-292 and pre-miR-293 have taken over specialized functions from the single human pre-miR-371 hairpin. [score:1]
The multiple sequence alignments of pre-miR-290–295/pre-miR-371–373 suggest that pre-miR-371 in the human cluster is capable of producing isomiRs with seeds corresponding to the mouse miR-292-3p0, miR-292-3p+1 and miR-293-3p+2, implied by the sequencing data. [score:1]
[1 to 20 of 22 sentences]
2
[+] score: 31
ch/ElMMo2/) of predicted target transcripts revealed a consensus set of regulated cellular functions for miR-291a-3p, miR-292-3p, miR-294 and miR-295, but not for miR-293 (Dataset S2 for the EIMMo target prediction software and Dataset S3 for the Pictar target prediction software). [score:8]
Dataset S2 Predicted target transcripts from the EIMMo target prediction software for miR-290 cluster (Sheet 1), miR-293 (Sheet 2), and miR-302 cluster (Sheet 3). [score:5]
Dataset S3 Predicted target transcripts from the Pictar target prediction software for miR-290 cluster (Sheet 1), miR-293 (Sheet 2), and miR-302 cluster (Sheet 3). [score:5]
Perhaps even more compelling, the analysis of the miR-290 cluster also revealed an unexpected expression profile for the highly abundant miR-293. [score:3]
Interestingly, all members of the miR-302 cluster share the same AAGUGC(U/C) 5′ seed sequence with the highly expressed members of the miR-290 cluster (with the notable exception, of course, of miR-293; Figure 2A–2B). [score:3]
Our study also underscores the benefit of unsupervised classification analyses in deciphering complex regulations of miRNAs cistrons, notably by uncovering outlier members within miRNA clusters, as shown here with the surprising findings made with miR-293. [score:2]
Thus, the most likely explanation to the result obtained in our analysis is a specific, post-transcriptional regulation of pre-miR-293 or mature miR-293. [score:2]
However, this could not be achieved with miR-293, indicating different functions for this specific miRNA. [score:1]
The miR-290 cluster comprises two pri-miRNA giving rise to six pre-miRNAs, of which pre-miR-293, premir-294 and premiR-295 are produced from the same primary transcript [17], [18]. [score:1]
Accordingly, a seed inspection uncovered a completely different sequence for miR-293 (Figure 2A, red), thereby confirming its singular status within the miRNA-290 cluster. [score:1]
[1 to 20 of 10 sentences]
3
[+] score: 11
In addition, Luningschror and his co-workers [29] have demonstrated that two members of this cluster, namely miR-291b-5p and miR-293, inhibit NF-κB signaling pathway through inhibition of p65. [score:5]
The other miRNAs of the miR-290-295 cluster (miR-290-5p, miR-291a-5p, miR-291b-5p, miR-292-5p, miR-293, miR-293 [*], miR-294 [*], and miR-295 [*]) differing in their seed sequences, are still highly expressed in ESCs with the exception of the hardly detectable [22] minor forms of miR-293, miR-294, and miR-295 (miR-293 [*], miR-294 [*], and miR-295 [*]). [score:3]
The miR-290-291 unit replication forms miR-292-291b, and then the miR-290, miR-291a and miR-292 (as the same unit) replication results in the formation of miR-293, miR-294 and miR-295, finally forming the present miR-290-295 cluster [21] (Fig.   1). [score:1]
The bold font are seed sequence, and the seed sequence of miR-293 is different from other members. [score:1]
miR-290-291a unit replication formed miR-292-291b, and then miR-290, miR-291a and miR-292 (as the same unit) replication resulted in the formation of miR-293, miR-294 and miR-295 ESC and iPSC self-renewals need to eliminate differentiation signal and obtain the pluripotency signal, in addition, the differentiation process trigger the closure of pluripotency procedure and the induction of lineage specification. [score:1]
[1 to 20 of 5 sentences]
4
[+] score: 5
Furthermore, miR-293 expression and seed sequence differs markedly from the other members of this family, indicating the need to re-examine previous inferences based on whole miR-290-295 overexpression studies [15]– [17]. [score:5]
[1 to 20 of 1 sentences]
5
[+] score: 4
We found that the mir-293-5p homologue, which was not previously identified in the rat, was upregulated in SHR livers. [score:4]
[1 to 20 of 1 sentences]
6
[+] score: 4
Members of the miR-290-295 cluster (miR-290, miR-291a, miR-292, miR-291b, miR-293, miR-294 and miR-295-1) were the most abundant among known miRNAs that were upregulated in rat PSCs. [score:4]
[1 to 20 of 1 sentences]
7
[+] score: 3
As expected from the microarray data, we verified that miR-125b-5p and miR-293 were significantly overexpressed in mouse brain and mouse ES cells, by 20.7-fold and 6.5-fold, respectively (Figure 7). [score:3]
[1 to 20 of 1 sentences]
8
[+] score: 3
Heatmap showing correct expression of control miRNA probe sets for muscle (mir-133a and mir-206) and ES cell control (mir-293 containing cluster). [score:3]
[1 to 20 of 1 sentences]
9
[+] score: 3
Other miRNAs from this paper: mmu-mir-9-2, mmu-mir-292a, mmu-mir-16-1, mmu-mir-124-1, mmu-mir-292b
We could confirm this expression pattern for mmu-mir-292 and mmu-mir-293 which are part of a larger pri-miRNA transcript detected exclusively in CCE cells (Figure 4B left panel). [score:3]
[1 to 20 of 1 sentences]
10
[+] score: 2
miR-291a-3p, miR-291a-5p, miR-292-3p, miR-290-5p and miR-293 belong to the miR-290-295 cluster [27]. [score:1]
Many embryo miRNAs, such as miR-293, miR-290-3p or miR-290-5p were in cluster C. Cluster C also included miR-106a which is believed to be both ES cell-specific and cardiac-related [26]. [score:1]
[1 to 20 of 2 sentences]