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25 publications mentioning mmu-mir-324

Open access articles that are associated with the species Mus musculus and mention the gene name mir-324. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 119
To further determine whether down-regulation of DICER could lead to cytoskeleton remo deling by suppression of miR-324-5p and hence restoration of its target proteins, R KO and HCT116 cells were transfected with siRNAs targeting DICER. [score:10]
More importantly, we found a key mechanism that Dicer suppresses cytoskeleton remo deling of colorectal epithelium by miR-324-5p mediated suppression of HMGXB3 and WASF-2. As one of most abundant miRNAs, miR-324-5p is significantly regulated by Dicer and has a key role in maintaining intestinal epithelial integrity and intestinal homeostasis. [score:6]
To further verify whether miR-324-5p could directly bind to the 3′UTR of HMGXB3 and WASF-2, HCT116 cells were co -transfected either miR-324-5p mimic or miR-324-5p inhibitor with 3′UTR promoter reporter plasmids bearing WT target sequences. [score:6]
Importantly, DICER deletion led to cytoskeleton remo deling and disruption of intestinal barrier by downregulation of miR-324-5p, which targets HMGXB3 and WASF-2. Based on these findings, we propose that DICER/miR-324-5p/ HMGXB3/WASF-2 axis plays a dominant role in cytoskeleton remo deling, intestinal barrier integrity maintaining and CRC tumorigenesis. [score:6]
Importantly, the elevated expressions of HMGXB3 and WASF-2 resulted from DICER knockdown could be alleviated by miR-324-5p overexpression (Figure 5E). [score:6]
To unravel the mechanisms by which miR-324-5p participates in maintaining intestinal barriers, we employed three miRNA targets predicting websites including TargetScan, miRBase and microRNA. [score:5]
Furthermore, administration of miR-324-5p agonist could successfully rescue cytoskeleton remo deling and inflammatory response resulted from Dicer dyregulation, suggesting that miR-324-5p is a potential target for prevention and treatment of CRC. [score:4]
HMGXB3 and WASF-2 are two direct targets of miR-324-5p. [score:4]
DICER deletion is accompanied with miR-324-5p downregulation. [score:4]
We found that HMGXB3 and WASF-2 are two direct targets involved in cytoskeleton remo deling of colorectal epithelium for miR-324-5p. [score:4]
HMGXB3 and WASF-2 are two direct targets for miR-324-5p. [score:4]
org to find the direct targets of miR-324-5p. [score:4]
B. Expression of DICER and hsa-miR-324-5p in DICER knockout cell lines. [score:4]
The luciferase reporter gene vectors including HMGXB3 and WASF-2 3′ untranslated region (3′UTR) sequences of the human miR-324-5p binding sites were constructed. [score:3]
G. A schematic mo del displaying the protective role of DICER/miR-324-5p in maintaining the intestinal epithelial integrity by targeting HMGXB3 and WASF-2. A. - B. Rhodamine phalloidin staining of Cacao2 cells was performed after 48h transfection with siDICER#2 and together with mimic-324-5p or not. [score:3]
A. Schematic diagram presenting the predicted miR-324-5p binding sequences for HMGXB3 and WASF-2. B. - C. Luciferase activity of HMGXB3 (B) and WASF-2 (C) 3′UTR constructs were analyzed after being transfected with miR-324-5p mimic or inhibitor. [score:3]
RT-PCR experiments validated decreased has-miR-324-5p expressions in all the three DICER deletion cell lines (Figure 3B). [score:3]
Conversely, the luciferase activities were increased by co -transfected with miR-324-5p inhibitor (Figure 5B and 5C). [score:3]
G. A schematic mo del displaying the protective role of DICER/miR-324-5p in maintaining the intestinal epithelial integrity by targeting HMGXB3 and WASF-2. To understand the clinical significance of DICER in CRC, DICER expression levels were evaluated in clinical samples of CRC patients. [score:3]
D. - E. Cells were transfected with siDICER#1 and #2 for 24h; DICER and has-miR-324-5p expression were analyzed by RT-PCR in R KO (D) and HCT116 (E) cells. [score:3]
G. A schematic mo del displaying the protective role of DICER/miR-324-5p in maintaining the intestinal epithelial integrity by targeting HMGXB3 and WASF-2. DICER, known as the RNase III endonuclease, plays a key role in the biogenesis of microRNAs (miRNAs), has been wi dely studied in many processes including mammalian embryogenesis [25, 26], DNA repair [27], genome stability [28] and different types of cancers. [score:3]
As shown in Figure 5A, both the has-miR-324-5p and its mouse homolog (mmu-miR-324-5p) share the identical sequence and are predicted to target WASF-2 and HMGXB3 within the 3′UTR region conservatively (Figure 5A). [score:3]
Figure 5 A. Schematic diagram presenting the predicted miR-324-5p binding sequences for HMGXB3 and WASF-2. B. - C. Luciferase activity of HMGXB3 (B) and WASF-2 (C) 3′UTR constructs were analyzed after being transfected with miR-324-5p mimic or inhibitor. [score:3]
Moreover, RT-PCR experiments showed that has-miR-324-5p mRNA levels were also significantly inhibited after siDICER transfection in R KO (Figure 3D) and HCT116 cells (Figure 3E). [score:3]
HMGXB3 and WASF-2, two proteins involved in cell motility and actin rearrangement were predicted as potential miR-324-5p targets. [score:3]
Our results, as summarized in Figure 6G, illustrate a key axis that contains DICER/miR-324-5p/ HMGXB3/WASF-2 in the epithelial cell cytoskeleton remo deling and dysregulated DICER/miR-324-5p/ HMGXB3/WASF-2 exacerbated gut inflammatory response and might lead to tumorigenesis of CRC. [score:2]
And, miR-324-5p mimic can effectively rescue the effects of DICER knockdown (Figure 6A) or deletion (Figure 6B) on cell polarity and cell tight-junction. [score:2]
MiR-324-5p mimic and inhibitor were purchased from RiboBio (Guangzhou, China). [score:2]
MiR-324-5p protects intestinal mucosal integrity through DCIER/ miR-324-5p/HMGXB3/WASF-2 axis. [score:1]
Importantly, we suggest a new DICER/miR-324-5p/HMGXB3/WASF-2 axis plays a dominant role in cytoskeleton remo deling, intestinal barrier integrity maintaining and tumorigenesis. [score:1]
For in vivo studies, the mmu-miR-324-5p agomir (modified miR-324-5p mimic) and the negative control were from RiboBio (Guangzhou, China). [score:1]
To explore the functional role of miR-324-5p in maintaining intestinal mucosal barrier, agomiR-324-5p was used to rescue the effects of Dicer deletion on intestinal injury and inflammatory response. [score:1]
MiR-324-5p agomiR antagonized inflammatory process in mice induced by DSS administration. [score:1]
In mice with IECs specific Dicer deficiency (Dicer [loxp/loxp]&Villin [Cre] and Dicer [loxp/+]&Villin [Cre]), mmu-miR-324-5p mRNA levels were also significantly decreased in the intestine epithelial layer (Figure 3C). [score:1]
C. mRNA levels of Dicer and mmu-miR-324-5p in Dicer [loxp/+] mice (n = 8), Dicer [loxp/+]&Villin [Cre] mice (n = 8) and Dicer [loxp/loxp]&Villin [Cre] mice (n = 8). [score:1]
We have found that DICER/miR-324-5p/HMGXB3/WASF-2 axis exists in vitro and in vivo. [score:1]
Taken together, our study demonstrated that miR-324-5p is a key player in maintaining intestinal barriers. [score:1]
Together, these data suggest that miR-324-5p is one of the key indispensible DICER downstream microRNAs involved in cytoskeleton remo deling. [score:1]
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2
[+] score: 30
We selected, for experimental validation, targets to three of these miRNAs: hsa-miR-17-5p (one target: see below), hsa-miR-15a (two targets), and hsa-miR-324-3p (three targets). [score:9]
In some cases the true miRNA found more target sites than did, on average, the corresponding controls (miR-324-3p, Figure 1D), whereas in other cases each control, on average, found more targets than did the miRNA (miR-129, Figure 1E). [score:5]
Numbers of predicted target sites per miRNA and its control sequences for (A) miR-1 and its controls with WC nt 2–8; if miR-1 hybridized with perfect WC complementarity this would yield −30.8 kcal/mol (see Methods); (B) let-7a and imposing only the requirement of WC base pairs within nucleotide positions 2–8; let-7a perfect WC complementarity would yield −33.2 kcal/mol; (C) miR-17-5p and its controls with WC nt 2–8; perfect WC complementarity would yield −44.5 kcal/mol; (D) miR-324-3p and its controls with WC nt 2–8; perfect WC complementarity would yield −52.8 kcal/mol; and (E) miR-129 and its controls with WC nt 2–8; perfect WC complementarity would yield −41.4 kcal/mol. [score:3]
0005745.g001 Figure 1Numbers of predicted target sites per miRNA and its control sequences for (A) miR-1 and its controls with WC nt 2–8; if miR-1 hybridized with perfect WC complementarity this would yield −30.8 kcal/mol (see Methods); (B) let-7a and imposing only the requirement of WC base pairs within nucleotide positions 2–8; let-7a perfect WC complementarity would yield −33.2 kcal/mol; (C) miR-17-5p and its controls with WC nt 2–8; perfect WC complementarity would yield −44.5 kcal/mol; (D) miR-324-3p and its controls with WC nt 2–8; perfect WC complementarity would yield −52.8 kcal/mol; and (E) miR-129 and its controls with WC nt 2–8; perfect WC complementarity would yield −41.4 kcal/mol. [score:3]
Five of the target sites (hsa-miR-15a/TSPYL2, hsa-miR-15a/BCL2, hsa-miR-17-5p/TNFSF12, hsa-miR-324-3p/CREBBP and hsa-miR-324-3p/WNT9B) exhibit perfect WC complementarity in the seed regions, while has-miR-324-3p/DVL2 has one GU pair in the same region (Supplementary Figure S1). [score:3]
This selection was made on the basis of functional association with cancer (hsa-miR-17-5p, hsa-miR-15a) or predicted targets in the Wnt signalling pathway (hsa-miR-324-3p) as described in Supplementary Table S7. [score:3]
Panels B, C and D of Figure 1 show the increasingly better energy-score ranges for predicted targets of let-7a, miR-17-5p and miR-324-3p. [score:3]
Although the binding site of hsa-miR-324-3p/DVL2 has very low (stable) free energy, the presence of a GU pair in the seed region means that binding at the 3′ end of the miRNA would have to be optimal to compensate (if indeed 3′-end hybridization can compensate at all); in this case, however, two unpaired bases and two GU pairs are predicted in the 3′ end, blocking even the possibility of adequate compensation. [score:1]
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3
[+] score: 22
Predicted targets of miR-762, miR-29a, and miR-324-5p were analyzed in the Sanger Center miRNA registry [19], TargetScan and PicTar target predictions [20]. [score:7]
In the present study, the expression of NKX2.5 was reduced in P19 cells treated with PCBs and NKX2.5 is a potential target of miR-324-5p. [score:5]
In the course of exploring the targets of these miRNAs by computational prediction, we found that Wnt1, GSK3β, and NKX2.5 were potential targets of miR-762, miR-29a, and miR-324-5p, respectively. [score:5]
This suggests that miR-324-5p might be important in controlling pathological events in congenital heart disease caused by exposure to PCBs. [score:3]
To validate the microarray results, eight miRNAs were selected for further experimental confirmation: miR-126-5p, miR-99a, miR-324-5p, miR-762, miR-29a, miR-302c, miR-295, miR-20b. [score:1]
Eight miRNAs (miR-126-5p, miR-99a, miR-324-5p, miR-762, miR-29a, miR-302c, miR-295, miR-20b) were randomly selected to confirm the microarray results using real-time RT-PCR. [score:1]
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4
[+] score: 9
Of the 186 miRNAs the expression of which was altered, nine were up-regulated at both time points (miR-125a-3p, miR-297c, miR-421, miR-452, miR-483, miR-574-3p, miR-574-5p, miR-669a, miR-720) and 11 were down-regulated at both time points (let-7g, miR-107, miR-10a, miR-15a, miR-15b, miR-199b*, miR-26a, miR-29c, miR-324-5p, miR-331-3p, miR-342-3p). [score:9]
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5
[+] score: 7
Furthermore, mirPRo reported that hsa-miR-324-5p is up-regulated significantly (see human data alignments in) whereas miRDeep2 determined that it does not have a significant differential expression. [score:6]
mmu-miR-152-3p exemplifies the case (Δ/−) while has-miR-324-5p exemplifies the case (Δ/+) shown in Fig. 2. The alignment results from miRDeep2 are presented in Supplementary Data 12– 21. [score:1]
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6
[+] score: 7
Macconi et al. 36, reported that high levels of miR-324-3p and the associated suppression of POP expression led to the suppression of low AcSDKP levels in urine and progressive renal damage in Munich Wistar Frömter (MWF) rats. [score:7]
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7
[+] score: 6
not significant Many Th1- and Th2-type inflammation-linked miRNAs, including miR-155, miR-146, and miR-324-5p, appear to be conserved in both mouse and human macrophages and are induced by LPS or IL-4, suggesting that the regulation of miRNA expression and function during classic or alternative macrophage activation is conserved [15, 18, 20, 24, 75]. [score:4]
We observed that the majority of IL-4-regulated miRNAs were strictly STAT6 -dependent in mouse macrophages, including miR-342-3p, miR-125a-5p, and miR-99b-5p, as well as the previously studied miR-511-5p and miR-324-5p. [score:2]
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8
[+] score: 6
In this study, five miRNAs (miR-29a, miR-29b, miR-126*, miR-127-3p, miR324-3p) were found upregulated and four (miR-188-5p, miR-25, miR-320a, miR-346) downregulated in both quiescent and active UC compared to healthy controls (Fasseu et al., 2010). [score:6]
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9
[+] score: 5
Other miRNAs from this paper: hsa-mir-324
2004.09.003 15380510 9. Song L. Liu D. Zhao Y. He J. Kang H. Dai Z. Wang X. Zhang S. Zan Y. Sinomenine inhibits breast cancer cell invasion and migration by suppressing NF-kappa B activation mediated by IL-4/miR-324-5p/CUEDC2 axisBiochem. [score:5]
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10
[+] score: 5
In this previous research, we showed that miR-324-5p and miR-326 targeting gli1 mRNA were highly expressed in activated LX2 cells. [score:5]
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11
[+] score: 4
044hsa-miR-18b2.20.014hsa-miR-423-5p1.70.048hsa-miR-932.10.014hsa-miR-1911.50.049hsa-miR-548b-5p2.30.015Downregulated miRNAs  hsa-miR-252.10.015hsa-miR-885-5p-4.20.00011hsa-miR-324-3p2.30.017hsa-miR-874-5.80.00018hsa-miR-3262.60.017hsa-miR-486-3p-4.60.00040hsa-miR-18a3.10.017hsa-miR-299-5p-4.20.0020hsa-miR-20b2.00.017hsa-miR-488-3.90.0063hsa-miR-1942.80. [score:4]
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12
[+] score: 4
The 10 most upregulated miRNAs were miR-146b, miR-297b, miR-34a, miR-469, miR-139-3p, miR-21, miR-466E-5p, miR22*, miR-324, and miR-143. [score:4]
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13
[+] score: 4
Out of these 68 miRNAs, two (miR-324 and miR-7) were found to be downregulated by ADAM8. [score:4]
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14
[+] score: 3
Other miRNAs from this paper: mmu-mir-133a-1, mmu-mir-133a-2
As epigenetic processes are presumably involved in memory (Molfese, 2011; Kaas et al., 2013; Landry et al., 2013; Lattal and Wood, 2013; Woldemichael et al., 2014; Yan et al., 2016), we are studying how these molecules, such as miRNA-324/miRNA-133a and their downstream targets, influence new synaptic innervations, recruit associative memory cells and induce plasticity in both sensory and motor cortices for memory establishment, extinction and reestablishment. [score:3]
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15
[+] score: 3
Two miRNAs, mmu-mir-324 and mmu-mir-423, lie within genes in which mutations were found, dishevelled 2 (Dvl2) and coiled-coil domain containing 55 (Ccdc55), respectively, though neither mutation identified in these genes is within the miRNA sequence itself. [score:3]
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16
[+] score: 3
Moreover, Bar and colleagues [33] found that the most overexpressed miRNAs in undifferentiated human ES cells are miR-302b, miR-302c, miR-302d, miR-92b, miR-20b, miR-519d, miR-302a, miR-324-3p, miR-187, and miR-18b. [score:3]
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17
[+] score: 2
Ten miRNAs, miR-20b, miR-145, miR-152, miR-155, miR-181a, miR-203, miR-222, miR-301a, miR-324-5p, and miR-351. [score:1]
Similar analysis of “late” group, including c-Maf, Ets1, N-Myc, Nfat5, and Nfib, yielded 10 miRNAs: miR-20b, miR-145, miR-152, miR-155, miR-181a, miR-203, miR-222, miR-301a, miR-324-5p, and miR-351, with multiple connections. [score:1]
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18
[+] score: 2
We and others have described several miRNAs that are involved in MB development, including miR-125b, miR-324-5p, miR-326 and miR-199b-5p [6], [7], [8]. [score:2]
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19
[+] score: 1
Most have not been associated with virus infection; however three, miR-122, miR-324 and let-7, have been identified in studies of host responses to viruses [34– 36]. [score:1]
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20
[+] score: 1
Other miRNAs from this paper: mmu-mir-133a-1, mmu-mir-133a-2
This assumption is granted by our current observation that anti-miRNA-324 and anti-miRNA-133a block associative memory and synapse innervation [38]. [score:1]
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21
[+] score: 1
Other miRNAs from this paper: mmu-mir-133a-1, mmu-mir-133a-2
Molecular mechanism for these processes on both sides remains to be studied, in which microRNA-324 and microRNA-133a appear involved (Wang et al., 2016). [score:1]
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22
[+] score: 1
Conversely, both mir-324-5p and mir-324-3p were placed into the "next" set and both mir-126-5p and-3p were placed into the "depleted" set; in each case, the pairs had very similar enrichment ratios, but only 324-5p and 126-3p were scored, to order to avoid counting the same pre-miR twice within the same sets. [score:1]
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23
[+] score: 1
Cells were irradiated using an X-RAD 320 X-Ray Biological Irradiator with a MIR-324 X-ray tube (Precision X-Ray Inc. [score:1]
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24
[+] score: 1
In the 67 APAP-early patients, miR-122-5p identified subsequent liver injury when normalized by any of the 6 endogenous miRNA normalizers described above (miR-122-5p area under ROC curve normalized by miR-1913, 0.97 (95% CI 0.92–1.01); miR-671, 0.96 (0.92–1.01); miR-1287, 0.95 (0.90–1.00); let7-d, 0.94 (0.89–1.00); miR-1260, 0.93 (0.88–1.00); miR-324, 0.93 (0.87–1.00) miR-122-5p ROC-AUC significantly larger than all other miRNAs – P < 0.05). [score:1]
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25
[+] score: 1
Other miRNAs from this paper: mmu-mir-133a-1, mmu-mir-133a-2
This assumption is supported by our current observation that anti-miRNA-324 and anti-miRNA-133a block associative memory and synapse innervation (Wang J. -H. et al., 2016; Wang et al., 2017). [score:1]
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