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5 publications mentioning rno-mir-339

Open access articles that are associated with the species Rattus norvegicus and mention the gene name mir-339. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 192
Other miRNAs from this paper: hsa-mir-339
Overexpression of miRNA-339 Upregulates the Acetylated NF- κB and FOXO1 Expression Level in SH-SY5Y CellsOur results showed that Sirt2 is the direct target of miR-339, indicating that miR-339 can contribute to an upregulation of the acetylated status of Sirt2 target, including NF- κB and FOXO1. [score:16]
Our present data showed that Sirt2 is likely a direct target of miRNA-339, which leads to a better explanation as to why acupuncture upregulated miRNA-339 expression companion with acupuncture downregulation of Sirt2 expression in hypertensive rats. [score:14]
To further validate whether knockdown of miRNA-339 can upregulate Sirt2 expression in rat neurons, we tested the effect of miRNA-339 on Sirt2 expression level in NGF -induced PC12 cells transfected with miRNA-339 inhibitor. [score:11]
Our results showed that Sirt2 is the direct target of miR-339, indicating that miR-339 can contribute to an upregulation of the acetylated status of Sirt2 target, including NF- κB and FOXO1. [score:9]
Overexpression of miRNA-339 Upregulates the Acetylated NF- κB and FOXO1 Expression Level in SH-SY5Y Cells. [score:8]
To further validate whether miRNA-339 could downregulate Sirt2 expression in human neurons, we tested the effect of miRNA-339 on Sirt2 expression level in SH-SY5Y cells transfected with miRNA-339 mimic. [score:8]
To further validate whether miRNA-339 can downregulate Sirt2 expression in rat neurons, we tested the effect of miRNA-339 on Sirt2 expression level in NGF -induced PC12 cells transfected with miRNA-339 mimic. [score:8]
Overexpression of miRNA-339 Downregulates Sirt2 Expression in Rat PC12 Cells. [score:8]
In this paper, we firstly found that one of the acupuncture-regulated targets, Sirt2, is likely a direct target of acupuncture-regulated miRNA-339 in neurons. [score:8]
Overexpression of miRNA-339 Downregulates Sirt2 Expression in Human SH-SY5Y Cells. [score:8]
To validate whether Sirt2 is a likely target of miR-339, we performed computational miRNA target analysis, which showed that miRNA-339 was able to bind to the Sirt2 mRNA 3′-UTR, suggesting this gene might be a potential target for miRNA-339 (Figure 1(a)). [score:7]
Taken together, we proposed that acupuncture exerts its therapeutic effects through a series of biological processes including (Figure 6) (1) changes of miRNAs (such as miR-339); (2) changes of targets (such as Sirt2); and (3) altered expression levels of Sirt2 activating its targets such as NF κB and FOXO1. [score:7]
Knockdown of miRNA-339 Upregulates Sirt2 Expression in Rat PC12 Cells. [score:7]
Cells were seeded in six-well plates at 1 × 10 [5] cells/well and transfected with different concentration of miRNA-339 mimic, miRNA-339 inhibitor, or nontargeting miRNA control (10 and 50 nM) using Lipofectamine 2000 (Invitrogen) according to the manufacturer's protocol. [score:5]
Overexpression of miRNA-339 significantly decreased Sirt2 expression in a dose -dependent (Figure 2(a), P < 0.01 versus control) and time -dependent manner (Figure 2(b), P < 0.05 versus control). [score:5]
The nontargeting miRNA control, miR-339 mimic, and miR-339 inhibitor were obtained from Invitrogen. [score:5]
Overexpression of miRNA-339 significantly decreased Sirt2 expression in a dose -dependent (Figure 3(a), P < 0.01 versus control) and time -dependent manner (Figure 3(b), P < 0.001 versus control). [score:5]
In addition, we demonstrated that altered Sirt2 expression by miR-339 activates its targets such as NF- κB and FOXO1 through increasing their acetylation. [score:5]
Moreover, to examine whether miR-339 could repress Sirt2 expression through direct 3′-UTR interaction, we cloned Sirt2 3′-UTR luciferase reporter plasmid and performed reporter analysis in SH-SY5Y cells. [score:4]
Knockdown of miRNA-339 significantly increased Sirt2 expression in a dose -dependent (Figure 4(a), P < 0.01 versus control) and time -dependent manner (Figure 4(b), P < 0.001 versus control). [score:4]
Overexpression and Knockdown of miR-339 via Transfection. [score:4]
There are three transcript isoforms of Sirt2 gene, including isoform 1 (43 kD), isoform 2 (39 kD), and isoform 5, but only 43 kD Sirt2 was affected after miR-339 overexpression or knockdown according to our present data. [score:4]
These data indicated that Sirt2 was likely a direct target of miR-339. [score:4]
Luciferase Assay Implies That Sirt2 Is Likely a Direct Target of miRNA-339 in SH-SY5Y Cells. [score:3]
Our findings implied that acupuncture may act through epigenetic changes and subsequent action on their targets, such as miRNA-339/Sirt2/NF- κB/FOXO1 axis. [score:3]
Our data also showed that miR-339 increased the acetylated NF- κB and FOXO1 expression level. [score:3]
Bioinformatics Studies and Selection of Sirt2 as a Potential Target of miR-339. [score:3]
Our present data demonstrated that cotransfection of miR-339 mimic with Sirt2 3′-UTR reporter resulted in dose -dependent inhibition of luciferase activity in SH-SY5Y cells (Figure 1(b), P < 0.05 versus control). [score:3]
As we expected, overexpression of miRNA-339 significantly increased the acetylation level of NF- κB and FOXO1 in a time -dependent manner (Figures 5(a) and 5(b), P < 0.01 versus control). [score:3]
The bioinformatics study showed that seven miRNAs, including miR-339, were predicted to regulate the human Sirt2 gene by the microRNA. [score:2]
Further studies are needed to validate whether miR-339 increased the acetylated NF κB and FOXO1 by regulating the protein levels, stability, or activity of Sirt2. [score:2]
In this study, we investigated if the regulation of sirtuin 2 (Sirt2) was regulated by miR-339 in human and rat neurons. [score:1]
However, miR-339 failed to repress the activity of Sirt2-3′-UTR reporter with a mutated miR-339 seed sequence (Figure 1(b)). [score:1]
To test this hypothesis, we measured the acetylated status of Sirt2 target (NF- κB and FOXO1) in SH-SY5Y cells treated with miRNA-339 mimic. [score:1]
A possible limitation of the present study was that some physiological level changes of neurons after altering the miR-339 levels are needed to validate the proposed therapeutic role of miR-339/Sirt2/NF κB/FOXO1 axis in response to acupuncture therapy in the future work. [score:1]
): sense, 5- CTAGTTTAACTCTTCCACGCCCGAGGGATCCA-3; and antisense, 5- AGCTTGGATCCCTCGGGCGTGGAAGAGTTAAa-3. SH-SY5Y cells were cultured in six-well plates at 1 × 10 [5] cells/well and transfected with different reporter vectors (300 ng Luc-Empty, 300 ng Luc-Sirt2-3′-UTR, or 300 ng Luc-Sirt2-3′-UTR-mut) and cotransfected with different concentration of miRNA-339 mimic (10 and 50 nM). [score:1]
): sense, 5- CTAGTTTAACTCTTCCACGCCCGAGGGATCCA-3; and antisense, 5- AGCTTGGATCCCTCGGGCGTGGAAGAGTTAAa-3. SH-SY5Y cells were cultured in six-well plates at 1 × 10 [5] cells/well and transfected with different reporter vectors (300 ng Luc-Empty, 300 ng Luc-Sirt2-3′-UTR, or 300 ng Luc-Sirt2-3′-UTR-mut) and cotransfected with different concentration of miRNA-339 mimic (10 and 50 nM). [score:1]
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2
[+] score: 32
Our data showed that acupuncture at taichong acupoint regulated miR-339, miR-223, and miR-145 expression, while acupuncture at nonacupoint failed to affect these miRNAs' expression. [score:6]
Moreover, compared to acupuncture at nonacupoint, acupuncture at taichong point significantly upregulated the expression of miRNA-339, miR-223, and miR-145 in medullas of SHRs (Figure 3). [score:5]
The data showed that miR-339, miR-223, and miR-145 were significantly upregulated in medullas of SHRs treated with acupuncture at taichong point in contrast to the mo del group untreated with acupuncture (Figure 2). [score:4]
For example, miRNA-339 has been shown to have a central role in regulating the expression of intercellular cell adhesion molecule-1 [39]. [score:4]
To validate the microarray profiling data, qRT-PCR was used to confirm the upregulated miRNAs including miRNA-339, miR-223, miR-145, and miR-451. [score:4]
miRNA-339, miR-223, and miR-145 are highly conserved and have multiple targets predicted for them in both humans and rats [39– 41]. [score:3]
Importantly, our RT-PCR assay has confirmed that miRNA-339, miR-223, and miR-145 were upregulated in SHRs treated with acupuncture at taichong acupoint in comparison with the nonacupoint group. [score:3]
While being compared to the normal control group (healthy SD rats), miR-339, miR-223, and miR-145 were significantly downregulated in mo del group. [score:3]
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3
[+] score: 8
Other miRNAs from this paper: mmu-mir-30a, mmu-mir-101a, mmu-mir-125a, mmu-mir-125b-2, mmu-mir-132, mmu-mir-134, mmu-mir-135a-1, mmu-mir-138-2, mmu-mir-142a, mmu-mir-150, mmu-mir-154, mmu-mir-182, mmu-mir-183, mmu-mir-24-1, mmu-mir-194-1, mmu-mir-200b, mmu-mir-122, mmu-mir-296, mmu-mir-21a, mmu-mir-27a, mmu-mir-92a-2, mmu-mir-96, rno-mir-322-1, mmu-mir-322, rno-mir-330, mmu-mir-330, mmu-mir-339, rno-mir-342, mmu-mir-342, rno-mir-135b, mmu-mir-135b, mmu-mir-19a, mmu-mir-100, mmu-mir-139, mmu-mir-212, mmu-mir-181a-1, mmu-mir-214, mmu-mir-224, mmu-mir-135a-2, mmu-mir-92a-1, mmu-mir-138-1, mmu-mir-181b-1, mmu-mir-125b-1, mmu-mir-194-2, mmu-mir-377, mmu-mir-383, mmu-mir-181b-2, rno-mir-19a, rno-mir-21, rno-mir-24-1, rno-mir-27a, rno-mir-30a, rno-mir-92a-1, rno-mir-92a-2, rno-mir-96, rno-mir-100, rno-mir-101a, rno-mir-122, rno-mir-125a, rno-mir-125b-1, rno-mir-125b-2, rno-mir-132, rno-mir-134, rno-mir-135a, rno-mir-138-2, rno-mir-138-1, rno-mir-139, rno-mir-142, rno-mir-150, rno-mir-154, rno-mir-181b-1, rno-mir-181b-2, rno-mir-183, rno-mir-194-1, rno-mir-194-2, rno-mir-200b, rno-mir-212, rno-mir-181a-1, rno-mir-214, rno-mir-296, mmu-mir-376b, mmu-mir-370, mmu-mir-433, rno-mir-433, mmu-mir-466a, rno-mir-383, rno-mir-224, mmu-mir-483, rno-mir-483, rno-mir-370, rno-mir-377, mmu-mir-542, rno-mir-542-1, mmu-mir-494, mmu-mir-20b, mmu-mir-503, rno-mir-494, rno-mir-376b, rno-mir-20b, rno-mir-503-1, mmu-mir-1224, mmu-mir-551b, mmu-mir-672, mmu-mir-455, mmu-mir-490, mmu-mir-466b-1, mmu-mir-466b-2, mmu-mir-466b-3, mmu-mir-466c-1, mmu-mir-466e, mmu-mir-466f-1, mmu-mir-466f-2, mmu-mir-466f-3, mmu-mir-466g, mmu-mir-466h, mmu-mir-504, mmu-mir-466d, mmu-mir-872, mmu-mir-877, rno-mir-466b-1, rno-mir-466b-2, rno-mir-466c, rno-mir-872, rno-mir-877, rno-mir-182, rno-mir-455, rno-mir-672, mmu-mir-466l, mmu-mir-466i, mmu-mir-466f-4, mmu-mir-466k, mmu-mir-466j, rno-mir-551b, rno-mir-490, rno-mir-1224, rno-mir-504, mmu-mir-466m, mmu-mir-466o, mmu-mir-466c-2, mmu-mir-466b-4, mmu-mir-466b-5, mmu-mir-466b-6, mmu-mir-466b-7, mmu-mir-466p, mmu-mir-466n, mmu-mir-466b-8, rno-mir-466d, mmu-mir-466q, mmu-mir-21b, mmu-mir-21c, mmu-mir-142b, mmu-mir-466c-3, rno-mir-322-2, rno-mir-503-2, rno-mir-466b-3, rno-mir-466b-4, rno-mir-542-2, rno-mir-542-3
Both ACTH and 17α-E2 up-regulated the expression of miRNA-212, miRNA-132, miRNA-154, miRNA-494, miRNA-872, miRNA-194, and miRNA-24-1, but reduced the expression of miRNA-322, miRNA-20b, miRNA-339, miRNA-27a, miRNA-551b, and miRNA-1224. [score:8]
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4
[+] score: 2
Among ncRNAs, some studies have reported that miRNAs are involved in the development of morphine tolerance [7, 8], including the let-7 family, miR-23b [9], miR-133b, miR-339 [10], miR-365 [11] and miR-219-5p [12]. [score:2]
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5
[+] score: 1
9 -45.6 mmu-miR-27b -1.8 -71.4 -462.7 mmu-miR-214* -2.6 -5.0 -43.5 mmu-let-7c-1* -73.2 -204.4 -334.1 mmu-miR-34c -9.4 -26.1 -42.7 mmu-miR-542–3p -5.9 -195.6 -319.8 mmu-miR-706 -9.3 -5.0 -38.7 mmu-miR-487b -2.0 -161.5 -263.9 mmu-miR-467b* -10.1 -2.2 -33.6 rno-miR-17–3p -1.6 -152.0 -248.5 mmu-miR-323–3p -3.7 -23.3 -29.8 mmu-miR-10b -2. 4 -136.6 -223.3 mmu-miR-202–3p -6.5 -5.9 -21.4 mmu-miR-29b -3.0 -135.1 -220.9 mmu-miR-339–5p -1.6 -9.6 -19.6 mmu-miR-297a* -2.4 -128.4 -209.8 mmu-miR-181c -2.0 -10.5 -14.6 mmu-miR-692 -41.5 -115.8 -189.2 mmu-miR-203 -4.6 -6.4 -13.8 mmu-miR-208 -40.6 -113.5 -185.5 mmu-miR-467a* -2.6 -3.9 -11.4 mmu-miR-467c -38.9 -108.6 -177. [score:1]
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