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13 publications mentioning rno-mir-216a

Open access articles that are associated with the species Rattus norvegicus and mention the gene name mir-216a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 41
Other miRNAs from this paper: rno-mir-217
RNA quality bias correction impacted the statistical analysis of miR-216a-5p and miR-217-5p so that significant expression change was not achieved although a significant expression change persisted for all but one of the target mRNAs; the expression of nhlrc2 was no longer significantly changed over any time points. [score:9]
The final objective was accomplished by demonstrating negative correlation between expression changes in miR-216a and miR-217 and expression changes in their predicted gene (mRNA) targets and then supporting these negative associations through the experimental literature. [score:7]
The short list of target genes for each miRNA identified by this stringent bioinformatics pipeline is of high quality and biological significance, as evidenced by the fact the vast majority (15 out of 17) of identified targets for miR-216a-5p and miR-217-5p turn out to be associated with tightly regulated pathways related to injury response. [score:6]
Relative to Fig.   3, these mRNAs would fall in clusters 1, 3, or 6. A focused pathway analysis of the down regulated genes associated with miR-216a-5p and miR-217-5p revealed that one mRNA (Pten) was a shared target of the two miRNAs and that all but two (Twistnb and Tmem178b) of the identified genes had some previously documented or hypothesized participation in pathways associated with cell autophagy and/or cell death stimulated by cellular stress (Fig.   6). [score:4]
The integration of target prediction, time relationship profiles, and known functional relationships promoted the authors’ confidence in the data particularly in review of that the findings for miR-216a-5p and miR-217-5p where a connection could be made to the experimentally observed histopathology. [score:3]
Differentially expressed mRNA during pancreatic injury sorted into these 6 temporal patterns Table 1 Top pathways identified in large miRNA-mRNA defined signaling network Pathway Source % of genes False discovery rate ErbB1 signaling NCI 1.0 1.2e-6 Signaling by NGF Reactome 3.8 7.2e-6 PIP3/AKT signaling Reactome 0.9 7.2e-6 Signaling by EGFR KEGG 0.7 6.2e-7 Signaling by SCF-KIT Reactome 2.9 2.7e-5 IL-2 signaling Reactome 2.6 2.7e-5 Signaling by PDGF NCI 0.5 2.7e-5 Signaling by ERBB4 Reactome 3.0 2.7e-5 Signaling by FGFR3 Reactome 2.6 2.7e-5 IL-6 signaling NCI 0.5 2.9e-5 Serum and tissue levels of two candidate miRNA biomarkers of acute pancreatic injury, miR-216a-5p and miR-217-5p, were compared across the time course (Additional file  4). [score:2]
This figure shows previously reported relationships between mRNAs associated with miR-216a-5p and miR-217-5p in this study and modulation of apoptosis and cell survival, the key histopathology findings in the current study. [score:1]
The delayed anti-correlation scores (see ) for the individual miRNA-mRNA relationships are presented in Table  3. In Fig.   2, miR-216a-5p and miR-217-5p would be found in the group depicted in the upper left in which miRNA values initially increase followed by a gradual decline to or below control levels. [score:1]
Two candidate miRNA biomarkers of pancreatic injury, miR-216a-5p and miR-217-5p, were, thus, strongly linked to mRNA for genes previously associated with autophagy and apoptosis. [score:1]
Although serum levels of miR-216a-5p and miR-217-5p increased dramatically, no significant corresponding changes in tissue levels were detected at early time points. [score:1]
This analysis reveals 9 mRNA negatively correlated with miR-216a-5p and 9 mRNA negatively correlated with miR-217-5p. [score:1]
In the specific cases of miR-216a-5p and miR-217-5p, the alignment of NGS described genes/pathways with the observed histopathology implies that the correction method eliminated information of a critical relationship in acinar cell injury response. [score:1]
These negative correlations are temporally depicted for miR-216a-5p and miR-217-5p in Figs.   4 and 5, respectively. [score:1]
The authors believe that this approach resulted in a list of high confidence pairs as demonstrated by the results for miR-216a-5p and miR-217-5p that were very strongly supported by histopathology findings. [score:1]
Confirmation of changes in respective protein levels or of the relationship of miR-216a-5p and miR-217-5p to autophagy and apoptosis or cell survival and death was not in the scope of this project. [score:1]
A more focused interrogation of the co-sequencing data revealed association of candidate miRNA biomarkers, miR-216a-5p and miR-217-5p, with specific mRNA of genes involved in cell survival or death largely through autophagy and apoptosis. [score:1]
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2
[+] score: 12
Amylase and lipase increases were noted from 1–8 h in rats in both 15 and 50 μg/kg dose groups while pancreatic necrosis was noted at 8, 24 and 48 h. MiR-375-3p has been reported to be enriched in islets and the miRNA with the highest intra-islet expression [38] and in our study was increased from 4–24 h in the 15 and 50 μg/kg groups, returning to approximately vehicle level by 48 h. MiR-216a-5p and miR-217-5p remained elevated in the serum of rats longer than amylase or lipase and had a much greater dynamic range which could be advantageous if detection of pancreatic injury is not able to be examined at earlier time points. [score:3]
Based on these studies miR-216a-5p displayed the largest dynamic range and correlated well with amylase and lipase indicating that it is the best translatable miRNA biomarker that correlated to pancreatic injury from rat to dog. [score:3]
MiR-216a-5p, amylase and lipase were increased in the serum concurrently at 1 h, remained elevated until 8 h while miR-216a-5p remained elevated until 24 h. MiR-217-5p displayed similar kinetics to miR-216a-5p except miR-217-5p generally had a larger dynamic range and remained elevated until 48 h. Both miRs-216a-5p and 217-5p displayed much larger dynamic ranges than amylase or lipase and remained elevated longer. [score:1]
Finally, we determined that miR-216a-5p appears to perform as well or better as a marker of pancreatic injury than amylase, lipase or other pancreas enriched miRNAs in rat and dog caerulein toxicity studies. [score:1]
It is possible that additional pancreas specific and/or enriched miRNAs may supplement the interpretation of pancreatic injury or serve as better biomarkers than miR-216a-5p in pancreatic toxicity studies utilizing different pancreatic toxicants. [score:1]
MiR-216b displayed similar kinetics to miR-216a-5p, but with a reduced dynamic range while miR-217-5p displayed increases similar to amylase. [score:1]
MiR-141-3p and miR-216a-5p were increased beyond vehicle treated dogs and did not display consistent time dependent increases. [score:1]
miRNAs were dose dependently increased in the serum of dogs treated with 15 and 45 ug/kg of caerulein and correlated to amylase and lipase increases while miR-216a-5p remained elevated until 24 h. Values are normalized to vehicle and to the cel-miR-55-3p spike in Conserved pancreas tissue enriched miRNAs were examined in the rat and dog mo dels of caerulein pancreatic toxicity in order to begin to characterize miRNAs as species translatable serum based biomarkers of pancreatic injury (Table  2). [score:1]
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3
[+] score: 10
miR-216a has been shown to target and decrease levels of Tslc1, whose expression is decreased in a number of tumors [45], thus implicating the sex-biased expression of miR-216a in carcinogenesis. [score:7]
Additionally, male-biased expression of miR-216a via the androgen pathway has been reported in early hepatocarcinogenesis [44]. [score:3]
[1 to 20 of 2 sentences]
4
[+] score: 8
miRNA Target Genes Pathways miR-128 ABCB9, BTG1, DSCR1, RASD1 ABC transporters General miR-136 GRN, PPP1R9B miR-147 HOXA1, PTGFRN miR-148 EGR3, SCN3A miR-181b IGF1R, NKX6-1 Adherens junction, Maturity onset diabetes of the, Focal adhesion, **Long term depression miR-196a ABCB9, CPB2, IRS1, MAPK10 ABC transporters General, Complement and coagulation cas, Adipocytokine signaling pathwa, Insulin signaling pathway, Type II diabetes mellitus, Fc epsilon RI signaling pathwa, Focal adhesion, **GnRH signaling pathway, **MAPK signaling pathway, Toll like receptor signaling p, Wnt signaling pathway miR-203 SARA1 miR-20 BTG1, SARA1, YWHAB Cell cycle miR-21 TPM1 mir-216 GNAZ **Long term depression miR-217 RHOA Adherens junction, Axon guidance, Focal adhesion, Leukocyte transendothelial mig, Regulation of actin cytoskelet, TGF beta signaling pathway, T cell receptor signaling path, Tight junction, Wnt signaling pathway miR-31 ATP2B2, DNM1L, EGR3, PPP1R9B, YWHAB **Calcium signaling pathway, Cell cycle miR-7 SLC23A2 miR-7b HRH3, NCDN, SLC23A2 **Neuroactive ligand receptor in b: miRNAs and their targets (from TargetScan and miRanda). [score:8]
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5
[+] score: 8
Serum was also analyzed by Q-RT-PCR for a panel of 20 tissue enriched and potential miRNA biomarkers, including those identified for liver (cfa-miR-122 and -885), heart/muscle (cfa-miR-1, -133, and -206), testis (miR-34b/c), pancreas (cfa-miR-216), brain (cfa-miR-212), and ubiquitously expressed cfa-miR-193b. [score:3]
Of these, only cfa-miR-216a correlated with observed expression in both mouse and rat pancreas [33]. [score:3]
A recent rat study demonstrated that plasma levels of miR-216a and miR-216b increased after pancreatic injury [34]. [score:1]
A total of 22 miRNAs (Additional file 6: Figure S5) were selected for qPCR validation including the following 14 biomarker candidates of organ toxicity: liver (cfa-miR-122 and -885), pancreas (cfa-miR-216a/b); heart (cfa-miR-499); muscle (cfa-miR-206); heart/muscle (cfa-miR-1, -133a/b, and -208); testis (cfa-miR-34b/c); and brain and sciatic nervous tissues (cfa-miR-212, -432, and -885), and 5 miRNAs reported in the literature (cfa-miR-21, -192, -193a/b, and -200). [score:1]
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6
[+] score: 7
Moreover, miR-377 is up-regulated in human and mouse mesangial cells exposed to high-glucose levels and can lead to increased fibronectin production in DN [13], while miR-216a regulates the collagen type I alpha 2 gene through mechanisms involving inhibition of the RNA binding protein Ybx1 [26]. [score:7]
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7
[+] score: 6
DOX -induced upregulation of miR-216 and miR-367 expression precedes the detection of overt histopathological lesions in cardiac tissue. [score:6]
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8
[+] score: 6
Current studies on diabetic processes indicate that miRNA-216, miRNA-217, and miRNA-21 target PTEN [6], and the miRNA-200 family targets friend of GATA (FOG)2 [32] to activate the Akt/mTOR signaling pathway, thereby mediating DN occurrence and development. [score:6]
[1 to 20 of 1 sentences]
9
[+] score: 3
Other miRNAs from this paper: rno-mir-291a, rno-mir-543, rno-mir-760, rno-mir-490, rno-mir-291b
Three miRNAs, miR-216a, miR-18b and miR-302a displayed elevated expression in both NLH and LH relative to control rats. [score:3]
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10
[+] score: 3
Previous studies have reported that miR-216a, miR-216b, and miR-217 are specifically expressed in the pancreas; these miRNAs were useful as biomarkers for pancreatic injury [30]. [score:3]
[1 to 20 of 1 sentences]
11
[+] score: 2
The miRNAs differentially regulated by prenatal stress includes miR-23a (up), miR-129-2 (up), miR-361 (down), let-7f (up), miR-17-5p (down), miR-98 (up), miR-425 (down), miR-345-5p (down), miR-9 (up), miR216-5p (up), miR-667 (up), and miR-505 (down) (Figure 3A). [score:2]
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12
[+] score: 2
Among them, miR-21-3p, miR216-5p, and miR542-3p are related to myocardial hypertrophy, cancer and apoptosis, respectively. [score:1]
Finally, as Table 1 showed, seven differentially expressed miRNAs were picked out complying with fold change (fc)≥4 (or ≤1/4) (both Sham group and YQFM group compared to MI group) and Reverse Rate (RR) (YQFM group compared to MI group) between 1 and 2. Finally, miR-21-3p, miR216-5p, miR219a-2-3p, miR381-3p, miR466c-5p, miR542-3p, and miR-702-5p were considered as the differentially reversed miRNAs regulated by YQFM. [score:1]
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13
[+] score: 2
Recently, some miRNAs for example miR-21, miR-1, miR-216[10], and miR-29 family[11], were reported to be deregulated in myocardial infarction. [score:2]
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