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9 publications mentioning gga-mir-19b

Open access articles that are associated with the species Gallus gallus and mention the gene name mir-19b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 80
ACSL1 is an endogenous target of miR-19b-3pAs shown in Fig. 5, ACSL1 was down-regulated, while gga-miR-19b-3p was up-regulated, in birds with high AbF. [score:9]
As shown in Fig. 5, ACSL1 was down-regulated, while gga-miR-19b-3p was up-regulated, in birds with high AbF. [score:7]
The effects of overexpressing gga-miR-19b-3p on preadipocyte proliferation were assessed using the Cell Counting Kit-8 (CCK-8, Dojindo Molecular Technologies, Kumamoto, Japan) at 24, 48, and 72 h. How to cite this article: Huang, H. Y. et al. Integrated analysis of microRNA and mRNA expression profiles in abdominal adipose tissues in chickens. [score:5]
From the analysis using the TargetScan and miRanda algorithms, ACSL1 transcripts may be a target of miR-19b-3p. [score:5]
Of particular note, there was a 12-fold up-regulation (five-fold in deep sequencing) of miR-19b-3p, and an eight-fold down regulation (six-fold in deep sequencing) of miR-122-5p between HAbF and LAbF chickens (P < 0.05). [score:5]
It is noteworthy that several of these miRNAs (gga-miR-17-5p, gga-miR-19a, gga-miR-19b and gga-miR-20a) were DEMs here (fold change 1.85 to 7.23) and were up-regulated in AbF from birds with high AbF contents. [score:4]
The miR17-92 cluster comprises seven miRNAs (miR-17-5p, miR-17-3p, miR-18a, miR-19a, miR-20a, miR-19b, and miR-92-1), and it has been shown to accelerate adipocyte differentiation by negatively regulating the tumor-suppressor Rb2/p130 32. [score:4]
The effects of overexpressing gga-miR-19b-3p on preadipocyte proliferation were assessed using the Cell Counting Kit-8 (CCK-8, Dojindo Molecular Technologies, Kumamoto, Japan) at 24, 48, and 72 h. The methods of this study were conducted in accordance with the Gui delines for Experimental Animals established by the Ministry of Science and Technology (Beijing, China). [score:3]
Of these, there was a striking difference (10 fold greater in birds with high AbF) in gga-miR-19b-3p expression, as quantified by qPCR, and the function of this miRNA in AbF is now being further studied. [score:3]
To further explore the biological significance of gga-miR-19b-3p, we observed the effect of gga-miR-19b-3p overexpression on the proliferation of preadipocytes and differentiation of adipocytes in chickens. [score:3]
Chicken acyl-CoA synthetase long-chain family member 1 (ACSL1) is a target for gga-miR-19b-3p. [score:3]
ACSL1 is an endogenous target of miR-19b-3p. [score:3]
Eleven of these (miR-204, miR-19a-3p, miR-19b-3p, miR-30d, miR-26a, miR-122-5p, miR-103-3p, miR-27b-3p, miR-92-3p, miR-142-3p, and miR-17-5p) have been implicated, directly or indirectly, in fat deposition; 9 showed a high fold-change (miR-3535, miR-144-3p, miR-30e-5p, miR-301b-3p, miR-215-5p, miR-200a-3p, miR-133a-3p, miR-133c-3p, and miR-146b-5p). [score:3]
Cells of the same group in six-well plates transfected with the gga-miR-19b-3p mimic were harvested and total RNA was extracted to identify the differential expression of ACSL1 mRNA. [score:3]
The changes in gga-miR-19b-3p expression after transfection with a gga-miR-19b-3p mimic, *** P < 0.001 vs. [score:3]
These results indicate that gga-miR-19b-3p contributes to the increased accumulation of AbF by down -regulating ACSL1 in chickens. [score:2]
When the adipocytes were treated with 50 nm of a gga-miR-19b-3p mimic, ACSL1 mRNA expression was significantly reduced compared with that of a negative control. [score:2]
Taken together, these results suggest that gga-miR-19b-3p accelerates the proliferation of preadipocytes, as well as adipocyte differentiation, by down- regulating ACSL1. [score:2]
Specifically, the integrated analysis of DEMs and DEGs suggests that nine miRNAs (gga-miR-19a-3p, miR-19b-3p, miR-17-5p, miR-30d, miR-26a, miR-103-3p, miR-27b-3p, miR-142-3p, and miR-92-3p) and three genes (ACSL1, FADS2 and ABCD3) are strong candidate miRNAs and genes involved in regulating the accumulation of AbF in chickens. [score:2]
As shown in Fig. 6, the expression of gga-miR-19b-3p, compared with the negative control, significantly increased after transfection with a gga-miR-19b-3p mimic (p < 0.001). [score:2]
After 24 h, cells were transfected with a gga-miR-19b-3p mimic (50 nM) or a negative control (50 nM) using 6 μL of FugeneHD. [score:1]
Oil Red O staining results showed that lipid droplets in cells treated with 50 nM of the gga-miR-19b-3p mimic were slightly larger and more numerous than those of the negative controls (Fig. 7C). [score:1]
Effect of gga-miR-19b-3p on the proliferation of preadipocytes and the differentiation of adipocytes. [score:1]
When the cells reached 60% to 70% confluence, pmirGLO-3′ UTR (100 ng) was co -transfected with 50 nM of a negative control or a gga-miR-19b-3p mimic (both from Ribo) using 0.25 μL of FugeneHD (Promega) according to the manufacturer’s instructions. [score:1]
Some of the DEMs identified by deep sequencing (miR-19a-3p, miR-19b-3p, miR-30d, miR-26a, miR-30a-5p, miR-122-5p, miR-103, miR-125b, and miR-17-5p) are known to influence mammalian lipid metabolism. [score:1]
At 48 h, the effect of the gga-miR-19b-3p mimic was significant (p < 0.05, Fig. 7A). [score:1]
This was explored via the co-transfection of luciferase reporter vectors containing the wild-type or mutant 3′ UTR of ACSL1 (Fig. 8A) and the miR-19b-3p mimic, or a negative control mimic, in 293T cells. [score:1]
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2
[+] score: 23
The up-regulation of miR-19a and miR-19b promotes cervical carcinoma cell proliferation and invasion by targeting CUL5 (Xu et al., 2012). [score:6]
MicroRNA and transcription factor co-regulatory network analysis reveals miR-19 inhibits CYLD in T-cell acute lymphoblastic leukemia. [score:4]
MicroRNA-19 (miR-19) regulates tissue factor expression in breast cancer cells. [score:4]
miR-19a and miR-19b over -expression in gliomas. [score:3]
miR-19 regulates the activity of the NF-κB signaling pathway to produce pro-inflammatory cytokines in inflammation (Gantier et al., 2012). [score:2]
gga-miR-19 target dual-luciferase reporter assay. [score:2]
A miR-19 regulon that controls NF-κB signaling. [score:1]
The Ct (2 [−ΔΔCt]) method was used to calculate relative expression of gga-miR-19, ZMYND11, NF-κ B, MyD88, and TNF-α (Livak and Schmittgen, 2001). [score:1]
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3
[+] score: 15
The expression level of miR-218-5p, miR-30-5p and miR-19-3p was downregulated by estrogen. [score:6]
As shown in Figure 3A, miR-218-5p, miR-30a-5p, miR-30b-5p, miR-30e-5p, miR-19a-3p, and miR-19b-3p were significantly downregulated in 17 β-estradiol treated groups (p < 0.01). [score:4]
Three miRNA clusters (miR-218-5p, miR-19-3p, and miR-30-5p) from 46 differentially expressed miRNA were found to potentially bind to the 3′UTR of ELOVL5 in the present study (Figure 2B). [score:3]
Among them, we found the seed sequences of miR-218-5p, miR-19a-3p, miR-19b-3p, miR-30a-5p, miR-30b-5p, miR-30d-5p, and miR-30e-5p were complementary with the 3′UTR of ELOVL5 (Figure 3A). [score:1]
Two miRNA (miR-124a-3p and miR-124b) and four miRNA clusters (miR-19-3p, miR-218-5p, miR-124-3p, and miR-30-5p) shared by three databases were found to be potentially combine the 3′UTR of ELOVL5, respectively (Figure 2A,B and Figure S3). [score:1]
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4
[+] score: 10
Currently, a few studies have indicated that TLRs themselves are directly targeted by miRNAs, and these interactions include the targeting of TLR2 by miR-105[11] and miR-19[48], the targeting of TLR4 by let-7i [10] and the targeting of TLR7 by miR-3148[49]. [score:10]
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5
[+] score: 9
As illustrated in Fig 3, 7 miRNAs including miR-29c, miR-217, miR-375, miR-215, miR-19b, miR-133a and let-7a had relatively low and stable expression levels (P < 0.05) in early period, and increased significantly (P < 0.01) from 12 to 13 weeks when the gonads entered into rapid development. [score:4]
Furthermore, miRNA-217, miRNA-155, miR-19b and miR-9 have target genes that are associated with puberty onset, such as FSHR, LEPR and circadian clock genes. [score:3]
According to previous reports and our sequencing results, 9 miRNAs, including miR-29c-3p, miR-375, miR-215-5p, miR-9-5p, miR-19b-3p, miR-133a-3p, let-7a, miR-217-5p and miR-155 were determined as candidates. [score:1]
Circulating miR-19b is a marker of fatty liver [47]. [score:1]
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6
[+] score: 8
A recent study showed that gga-miR-19b-3p, a member of the miR-17-92 cluster, was significantly differentially expressed in preadipocytes between chickens with high and low abdominal adipose weight, and further studies showed that gga-miR-19b-3p targeted ACSL1 (acyl-CoA synthetase long-chain family member 1), and overexpression of gga-miR-19b-3p promoted chicken preadipocyte proliferation and differentiation [31]. [score:7]
Wu Q MiR-19b/20a/92a regulates the self-renewal and proliferation of gastric cancer stem cellsJ. [score:1]
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7
[+] score: 6
Other miRNAs which are expressed at high levels in HD11 include gga-miR-142-3p (10.5%), gga-miR-223 (6.9%), gga-miR-19b (4%), gga-miR-20a (3.7%) and gga-miR-22 (3.4%). [score:3]
Other chicken miRNAs that are expressed at high levels in IAH30 cells include gga-miR-24 (5.5%), gga-miR-27b (4%), gga-miR-19b (3.9%), gga-miR-20a (3.9%), gga-miR-148a (3.7%), gga-miR-23b (3%), and gga-miR-92 (3%). [score:3]
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8
[+] score: 6
3.4Wang et al. identified 32 known miRNAs from skeletal muscle of Arbor Acres commercial chickens, of which 12 form five clusters: miR-133a-1-miR-1a-2, miR-23b-miR-24, miR-99a-let-7c, miR-92-miR-19b-miR-18a-miR-17, and miR-30e-miR-30c-1, suggesting that most miRNAs co-express in skeletal muscle [85]. [score:3]
Wang et al. identified 32 known miRNAs from skeletal muscle of Arbor Acres commercial chickens, of which 12 form five clusters: miR-133a-1-miR-1a-2, miR-23b-miR-24, miR-99a-let-7c, miR-92-miR-19b-miR-18a-miR-17, and miR-30e-miR-30c-1, suggesting that most miRNAs co-express in skeletal muscle [85]. [score:3]
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9
[+] score: 1
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-17, hsa-mir-18a, hsa-mir-19a, hsa-mir-19b-1, hsa-mir-19b-2, hsa-mir-20a, hsa-mir-21, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-106a, hsa-mir-16-2, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181a-1, hsa-mir-221, hsa-mir-222, hsa-mir-223, hsa-let-7g, hsa-let-7i, hsa-mir-15b, hsa-mir-23b, hsa-mir-27b, hsa-mir-122, hsa-mir-125b-1, hsa-mir-140, hsa-mir-125b-2, hsa-mir-136, hsa-mir-146a, hsa-mir-150, hsa-mir-206, hsa-mir-155, hsa-mir-181b-2, hsa-mir-106b, hsa-mir-302a, hsa-mir-34b, hsa-mir-34c, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, hsa-mir-367, gga-let-7i, gga-let-7a-3, gga-let-7b, gga-let-7c, gga-mir-125b-2, gga-mir-155, gga-mir-222a, gga-mir-221, gga-mir-92-1, gga-mir-20a, gga-mir-19a, gga-mir-18a, gga-mir-17, gga-mir-16-1, gga-mir-15a, gga-mir-1a-2, gga-mir-206, gga-mir-223, gga-mir-106, gga-mir-302a, gga-mir-181a-1, gga-mir-181b-1, gga-mir-16-2, gga-mir-15b, gga-mir-140, gga-let-7g, gga-let-7d, gga-let-7f, gga-let-7a-1, gga-mir-146a, gga-mir-181b-2, gga-mir-181a-2, gga-mir-1a-1, gga-mir-1b, gga-let-7a-2, gga-mir-34b, gga-mir-34c, gga-let-7j, gga-let-7k, gga-mir-23b, gga-mir-27b, gga-mir-24, gga-mir-122-1, gga-mir-122-2, hsa-mir-429, hsa-mir-449a, hsa-mir-146b, hsa-mir-507, hsa-mir-455, hsa-mir-92b, hsa-mir-449b, gga-mir-146b, gga-mir-302b, gga-mir-302c, gga-mir-302d, gga-mir-455, gga-mir-367, gga-mir-429, gga-mir-449a, hsa-mir-449c, gga-mir-21, gga-mir-1458, gga-mir-1576, gga-mir-1612, gga-mir-1636, gga-mir-449c, gga-mir-1711, gga-mir-1729, gga-mir-1798, gga-mir-122b, gga-mir-1811, gga-mir-146c, gga-mir-15c, gga-mir-449b, gga-mir-222b, gga-mir-92-2, gga-mir-125b-1, gga-mir-449d, gga-let-7l-1, gga-let-7l-2, gga-mir-122b-1, gga-mir-122b-2
Within these clusters, the mir-92-mir-19b-mir-20a-mir-19a-mir-18a-mir-17, which is equivalent to the mammalian mir-17-92 cluster, and mir-302b-mir-302c-mir-1811-mir-302a-mir-302d-mir-367 cluster were the biggest clusters containing six miRNAs. [score:1]
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