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22 publications mentioning gga-mir-30b

Open access articles that are associated with the species Gallus gallus and mention the gene name mir-30b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

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[+] score: 118
7-A, miR-30b expression profile comparison; 7-B, miR-30c expression profile comparison; 7-C, CARS mRNA expression profile comparison. [score:7]
Four up-regulated miRNAs (gga-miR-221, gga-miR-30b, gga-miR-30c and gga-miR-215) and 1 down-regulated miRNA (gga-miR-375) in RSS chicken were chosen randomly to validate the Solexa sequencing results using RT-qPCR. [score:7]
CARS expression varied inversely with miR-30b/c expression at 4 w and 6 w of age, although the differences did not attain to statistical significance (P > 0.05) (Fig 9). [score:5]
Secondly, we detected the miR-30b/c and CARS expression in pectoral muscle, thigh muscle, liver, hypothalamus and pituitary of chicken at 7 w of age and found that miR-30b in thigh muscles and CARS in hypothalami were differentially expressed between RSS and normal chickens (Fig 7). [score:5]
The wild-type and mutated target sequences of CARS 3′UTR predicted as target of gga-miR-30b/c was amplified by PCR from chicken cDNA and inserted into the downstream of luciferase reporter gene in the pmirGLO vector (Promega Biotech Co. [score:5]
Subsequently, qPCR was conducted to compare the expression of miR-30b/c and CARS in thigh muscle and hypothalami between RSS and normal chickens at various developmental stages (1 d, 2w, 4wand 6w of age). [score:4]
2.6. gga-miR-30b/c directly target CARS through binding to its 3′UTR. [score:4]
These results indicated that CARS 3′UTRwas directly targeted by miR-30b/c. [score:4]
The inhibitory relationship between gga-miR-30b/c and their predicted target gene CARS was verified using luciferase reporter assays. [score:4]
Temporal and spatial expression of miR-30b/c and CARS gene in RSS chickensWe firstly compared the miR-30b/c and CARS expression between normal and RSS chicken livers at three time points (Fig 6). [score:4]
T-test was used to make miR-30b/c and CARS temporal and spatial expression comparisons between RSS and normal chickens. [score:3]
The pri-miR-30b/c and the flanking sequences, obtained by PCR from chicken DNA, were inserted into pCDNA3.1+ expression vector (Invitrogen) and subsequently were described as pCDNA3.1-miR-30b/c. [score:3]
“*” represents miR-30b/c or CARS was differentially expressed between RSS and normal chickens (P < 0.5). [score:3]
In short, abnormal expression of gga-miR-30b/c and CARS might lead to RSS chickens by affecting liver metabolism and function. [score:3]
gga-miR-30b/c and CARS in liver showed inversed expression trends, especially at age of 6w (P < 0.05). [score:3]
Members of the miR-30 family function in osteoblast differentiation, adipogenic differentiation, angiogenesis, myocardial matrix remo deling and cell apoptosis by targeting various pathways [18– 22, 33]. [score:3]
“*” represents miR-30b or c was differentially expressed between RSS and normal chickens (P < 0.5). [score:3]
Temporal and spatial expression of miR-30b/c and CARS gene in RSS chickens. [score:3]
6-A, comparison of miR-30b expression in livers. [score:3]
Comparison of miR-30b/30c expression in thigh muscles and hypothalami of RSS and normal chickens at day 1 (D1), 2, 4 and 6 w of age. [score:3]
0127342.g006 Fig 6Comparison of the miR-30b/c and CARS expression in livers of RSS and normal chickens at 4, 5 and 6 w of age. [score:3]
The CARS gene was proven as the target of miR-30b/c. [score:3]
qPCR results indicated that miR-30b/c were expressed in a higher level in RSS chicken than in the normal chickens, while CARS showed in an inversed trend. [score:3]
Comparison of the miR-30b/c and CARS expression in livers of RSS and normal chickens at 4, 5 and 6 w of age. [score:3]
Hence, further studies are needed to clarify the role of miR-30b/c in regulating muscle development. [score:3]
Dual-luciferase reporter assay for the 3′ UTR of CARS as the target site of miR-30b/c in vitro. [score:2]
Also, gga-miR-30b/c expression was increased in the thigh muscle and hypothalamus in RSS chickens compared with normal chickens at the indicated time points. [score:2]
We firstly compared the miR-30b/c and CARS expression between normal and RSS chicken livers at three time points (Fig 6). [score:2]
pCDNA3.1-miR-30b/c plasmid were cotransfected with the pmirGLO- CARS 3′ UTR, pmirGLO- CARS 3′UTR mutation, or the control plasmid into DF-1 cells. [score:2]
Quantitative RT-PCR assays of miR-30b and miR-30c expression using total RNA isolated from the chicken thigh muscle and hypothalamus at day1 (D1), 2, 4 and 6 w of age. [score:2]
In this study, gga-miR-30b/c expression was increased in liver in RSS chickens compared with normal chickens. [score:2]
0127342.g005 Fig 5Dual-luciferase reporter assay for the 3′ UTR of CARS as the target site of miR-30b/c in vitro. [score:2]
From 2 to 6 w, miR-30b/c expression was increased in the thigh muscles and hypothalami of RSS chickens compared with normal chickens, and it is notable that significant differences (P < 0.05) appeared at 4 w of age (Fig 8). [score:2]
0127342.g008 Fig 8Quantitative RT-PCR assays of miR-30b and miR-30c expression using total RNA isolated from the chicken thigh muscle and hypothalamus at day1 (D1), 2, 4 and 6 w of age. [score:2]
0127342.g007 Fig 7Comparisons of CARS mRNA and miR-30b/c in various tissues between RSS and normal chickens at 7 w of age. [score:1]
Gga-miR-30b and gga-miR-30c share the same seed regions and belong to the gga-miR-30 family. [score:1]
, China), We co -transfected 200 ng pmirGLO-CARS or pmirGLO-CARS mutant, with 600 ng pCDNA3.1-miR-30b/c plasmid, into DF-1 cells. [score:1]
P value of miR-30b at 6 w of age was 0.08, very close to the statistically significant level (Fig 6-A). [score:1]
pmirGLO-CARS and pCDNA3.1-miR-30b/c plasmids were cotransfected into chick DF-1 cell lines with lipofectamine 3000 reagent (Invitrogen). [score:1]
Comparisons of CARS mRNA and miR-30b/c in various tissues between RSS and normal chickens at 7 w of age. [score:1]
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2
[+] score: 56
Another approach to multiple siRNA expression was stimulated by report that a mouse miR30 -based shRNA expression cassette can be driven by Pol II promoters and provide higher knockdown efficiency than those driven by the Pol III U6 promoter [10]. [score:6]
These results suggest that although NP miRNA can be expressed from the mouse miR30 -based cassette in DF-1 cells, the level of target gene knockdown is modest following stable integration of the lentiviral vector. [score:6]
Subsequently, Sun et al showed that a single Pol II promoter can drive three artificial miR30 cassettes to express siRNAs all targeting GFP, resulting in further knockdown of the GFP intensity in the cells [17]. [score:6]
The mouse miR30 -based miRNA expression cassette has been wi dely used to express artificial miRNA in lentiviral vectors [21]. [score:5]
As shown in Figure 1c, transient expression of miR30-NP inhibited Renilla luciferase activity by ∼85%. [score:5]
Inhibition of luciferase activity by NP miRNA expressed from a mouse miR30 -based lentiviral vector. [score:5]
To express anti-influenza artificial miRNA, we replaced the mature miR30 sequences in pLB2 with sequences that target nucleoprotein (NP) of influenza virus (Figure 1b). [score:5]
As a control, Vero cells were transduced with a CPGM lentivirus that expressed miR30 -based miRNA specific for the firefly luciferase transcript. [score:3]
Expression of NP miRNA from the mouse miR30 -based lentiviral vector. [score:3]
Zhou et al reported that two tandem copies of the miR30 -based cassette can be expressed in a single transcript driven by a Pol II promoter [15], [16]. [score:3]
In addition to miR30 -based designs, mouse miR155 -based design has also been used to knockdown multiple genes [19]. [score:2]
In the transient transfection assay, the miR30-NP lentiviral vector and psicheck-2 dual luciferase reporter plasmid, in which the NP target sequence was cloned into the 3′ UTR of the synthetic Renilla luciferase gene, were co -transfected into DF-1 cells. [score:2]
A similar miR30 -based approach was utilized by Zhu et al to knockdown multiple genes [18]. [score:2]
Flanking and hairpin sequences are miR30. [score:1]
0022437.g001 Figure 1(a) Schematic diagram of the miR30-NP lentiviral vector. [score:1]
Psicheck-2 dual luciferase reporter plasmid (50 ng) and miR30-NP lentiviral vector (450 ng) were co -transfected in DF-1 cells. [score:1]
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[+] score: 16
Of the differentially expressed miRNAs, expression of let-7b, miR-30a-5p, miR-30b, miR-99a, and miR-133b was commonly up-regulated in both dwarf and normal chickens. [score:8]
In 7-week-old chickens, as compared with 14-day-old embryos, the expression of let-7b, miR-30a-5p, miR-30b, miR-99a and miR-133b was significantly up-regulated, but miR-16c, miR-92, miR-106, miR-203, miR-451 and miR-454 were significantly down-regulated in both dwarf and normal chickens. [score:8]
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[+] score: 15
The expression level of miR-218-5p, miR-30-5p and miR-19-3p was downregulated by estrogen. [score:6]
As shown in Figure 3A, miR-218-5p, miR-30a-5p, miR-30b-5p, miR-30e-5p, miR-19a-3p, and miR-19b-3p were significantly downregulated in 17 β-estradiol treated groups (p < 0.01). [score:4]
Three miRNA clusters (miR-218-5p, miR-19-3p, and miR-30-5p) from 46 differentially expressed miRNA were found to potentially bind to the 3′UTR of ELOVL5 in the present study (Figure 2B). [score:3]
Two miRNA (miR-124a-3p and miR-124b) and four miRNA clusters (miR-19-3p, miR-218-5p, miR-124-3p, and miR-30-5p) shared by three databases were found to be potentially combine the 3′UTR of ELOVL5, respectively (Figure 2A,B and Figure S3). [score:1]
Among them, we found the seed sequences of miR-218-5p, miR-19a-3p, miR-19b-3p, miR-30a-5p, miR-30b-5p, miR-30d-5p, and miR-30e-5p were complementary with the 3′UTR of ELOVL5 (Figure 3A). [score:1]
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[+] score: 12
Other down-regulated miRNAs include members of the miR-30 family of miRNAs (gga-miR-30c, gga-miR-30d, gga-miR-30a-5p) many of which have been implicated in a wide range of cancers (Gaziel-Sovran et al., 2011; Baraniskin et al., 2012; Cheng et al., 2012). [score:4]
miR-30b/30d regulation of GalNAc transferases enhances invasion and immunosuppression during metastasis. [score:4]
Other downregulated miRNAs included members of the miR-30 family of miRNAs (gga-miR-30c, gga-miR-30d, gga-miR-30a-5p) and the avian-specific gga-miR-1729 originally discovered in developing chick embryo. [score:4]
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[+] score: 12
Of the seventeen miRNAs that are expressed at higher levels at the later stage of development (HH25) in the chicken, duck, and quail (Table S7) four belong to the miR-30 family (miR-30a-3p, miR-30a-5p, miR-30d*, and miR-30e*). [score:4]
Taken together, these miRNA changes, including differential expression of let-7, miR-302, and miR-30 families (Table S7), indicate that the HH20 to HH25 developmental window may be a critical transition phase in which multipotent NC cells begin to differentiate to form the various tissues of the face. [score:4]
Thus, up-regulation of the miR-30 family might reflect an increase in cellular differentiation at HH25. [score:4]
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[+] score: 11
By using three different endogenous control miRNAs (U6, 5S, and 18S), the results have all showed that two miRNAs (gga-miR-181b-5p_R + 1 and gga-miR-30b-5p) had significantly lower expression levels in matured livers than the postnatal livers, and the expression level of another four miRNAs (gga-let-7a-5p, gga-miR-2954_R + 2, gga-miR-21-5p, and gga-miR-122-5p) were significantly increased in matured livers (Fig.   3), which were consistent with the miRNA-seq data. [score:5]
Moreover, we also found that miRNA-30 family and let-7 family were significantly differentially expressed between the postnatal and matured livers and were abundant in the liver, whose important roles in the liver development have been proved 14, 22. [score:4]
Hand NJ The microRNA-30 family is required for vertebrate hepatobiliary developmentGastroenterology. [score:2]
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[+] score: 10
Some miRNAs had only two viral targets, such as gga-mir-32 (targeting HA and NS genes) and gga-miR-30b (targeting M and NA genes). [score:7]
: AY278204), has predicted binding sites for seven differentially expressed miRNAs: gga-miR-30b, 34a, 142-5p, 202, 460b-5p, 449b, and 460a. [score:3]
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[+] score: 10
For example, a comparison of miRNA expression profiles in proliferating myoblasts and differentiated myotubes revealed that miR-221 and miR-222 are down-regulated upon differentiation of primary and established myogenic cells, whereas miR-21, miR-103, miR-130, miR-99, miR-30 and miR20 are up-regulated [19, 33], suggesting that these miRNAs play important roles in the transition between proliferation and differentiation of muscle cells. [score:9]
In addition to miR-206, miR-1 and miR-181, nine other miRNAs among the most abundant in these libraries (miR-221, miR-222, miR-21, miR-103, miR-130, miR-99, miR-30, miR20, and miR128) have been implicated in the proliferation and differentiation of muscle cells (Table 1) [15, 19, 33]. [score:1]
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10
[+] score: 8
Other miRNAs from this paper: gga-mir-194, gga-mir-10b, gga-mir-29c, gga-mir-143
The array results demonstrated that the expression levels of miR-10b, miR-192, miR-194, and miR-197 were up-regulated more than 10-fold; whereas those of miR-143, miR-150, miR-28, miR-29c, miR-30b, miR-320a, miR-328, and miR-451a were up-regulated 5- to 10-fold in the glutamate -treated group as compared to α-MSH + glutamate -treated group (Supplementary Table S3, Fig. 5A). [score:8]
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11
[+] score: 6
In addition, the putative target genes of miR-30 family members (miR-30a, -30b, -30d, -30e, and -30c) encode proteins involved in glycerophospholipid metabolism, biosynthesis of unsaturated fatty acids, pantothenate and CoA biosynthesis, and PPAR signalling pathway. [score:3]
Members of the miR-30 family are important positive regulators of adipocyte differentiation in a human adipose tissue-derived stem cell mo del 44. [score:2]
Seven conserved families all were DE with P ≤ 0.05, including let-7 (let-7a, -7b, -7c,-7f, -7g, -7i, -7j, and -7k), miR-130 (miR-130a, and -130b), miR-146 (miR-146a, -146b, and -146c), miR-15 (miR-15a, -15b, and -15c), miR-181 (miR-181a and -181b), miR-29 (miR-29a, -29b and -29c), and miR-30 (miR-30a, -30b, -30c, -30d, and -30e). [score:1]
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12
[+] score: 4
Other miRNAs from this paper: hsa-let-7d, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-21, hsa-mir-22, hsa-mir-30a, hsa-mir-32, hsa-mir-33a, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-147a, hsa-mir-34a, hsa-mir-187, hsa-mir-204, hsa-mir-205, hsa-mir-200b, hsa-mir-23b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-138-2, hsa-mir-142, hsa-mir-144, hsa-mir-125b-2, hsa-mir-138-1, hsa-mir-146a, hsa-mir-190a, hsa-mir-200c, hsa-mir-155, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-30e, hsa-mir-365b, hsa-mir-328, gga-mir-33-1, gga-mir-125b-2, gga-mir-155, gga-mir-17, gga-mir-148a, gga-mir-138-1, gga-mir-187, gga-mir-32, gga-mir-30d, gga-mir-30a, gga-mir-30c-2, gga-mir-190a, gga-mir-204-2, gga-mir-138-2, gga-let-7d, gga-let-7f, gga-mir-146a, gga-mir-205b, gga-mir-200a, gga-mir-200b, gga-mir-34a, gga-mir-30e, gga-mir-30c-1, gga-mir-205a, gga-mir-204-1, gga-mir-23b, gga-mir-142, hsa-mir-449a, hsa-mir-489, hsa-mir-146b, hsa-mir-548a-1, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-33b, hsa-mir-449b, gga-mir-146b, gga-mir-147, gga-mir-489, gga-mir-449a, hsa-mir-449c, gga-mir-21, gga-mir-144, gga-mir-460a, hsa-mir-147b, hsa-mir-190b, gga-mir-22, gga-mir-460b, gga-mir-1662, gga-mir-1684a, gga-mir-449c, gga-mir-146c, gga-mir-449b, gga-mir-2954, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548ab, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-548am, hsa-mir-548an, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-548ay, hsa-mir-548az, gga-mir-365b, gga-mir-33-2, gga-mir-125b-1, gga-mir-190b, gga-mir-449d, gga-mir-205c
And Caruso et al firstly reported miRNAs dysregulation in the progress of PAH by the means of microarray analysis and quantitative polymerase chain reaction, who detected that miR-451and miR-322 up regulated, whereas miR-30, miR-22, and let-7f down regulated in PAH rodent mo dels [27]. [score:4]
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13
[+] score: 4
The present study of splenic miRNA and mRNA profiles from chickens after Salmonella challenge has identified differential expression of several miRNAs linked to immune responses, including miR-155, miR-9, miR-30 which have been reported previously and several miRNAs, such as miR-101-3p and miR-130b-3p, which were shown here to be associated with the immune response to infection with SE. [score:3]
Several miRNAs previously reported to be involved in immune responses such as miR-155, miR-9, miR-30, miR-126, and miR-29 families were identified. [score:1]
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14
[+] score: 3
Most of the changed microRNAs are involved in development (especially in the embryonic development) and some examples of them are miRNA-222, miRNA-383, miRNA-126, miRNA-133, miRNA-30, miRNA-10a, miRNA-196 and miRNA-18b. [score:3]
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[+] score: 2
The mir-30 family of miRNAs have been shown to be involved in early muscle development in zebrafish [18] and the mouse equivalent (mmu-mir-100-5p) of gga-mir-100-5p is involved in embryonic stem-cell differentiation [19]. [score:2]
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16
[+] score: 2
Other miRNAs from this paper: gga-mir-30d, gga-mir-30a, gga-mir-30c-2, gga-mir-30e, gga-mir-30c-1
Following PCR to form the siRNA with hairpin primers, the siRNA PCR fragment was then cloned into the miRNA30-like hairpin insertion site in a slx12 shuttle vector containing RFP, along with a U6 siRNA cassette containing the chicken U6 promoter and a microRNA operon expression cassette. [score:2]
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17
[+] score: 2
Other miRNAs from this paper: gga-mir-30d, gga-mir-30a, gga-mir-30c-2, gga-mir-30e, gga-mir-30c-1
Gene-specific shRNAs are embedded into the primary transcript of human miR30, which is located in the 3′UTR of a doxycycline-regulated GFP transgene. [score:2]
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18
[+] score: 1
In addition to these transcripts our data list microRNAs 490-5p&3p, 1677-5p, 126-5p, 1434-5p, 193b-3p, and the members of the miR30 family, i. e., 30a-5p, 30b-5p, 30d-5p and 30e-5p, as novel cardiac enriched transcripts. [score:1]
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19
[+] score: 1
A number of miRNA backbones can be used, including miR-155, miR-30 and miR17-92 [23- 25]. [score:1]
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20
[+] score: 1
Similarly, other miRNA families exhibited vast read ranges, such as the gga-miR146 (91–235,844 reads) and gga-miR-30 (886–164,361 reads) families (Additional file 1: Table S2). [score:1]
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21
[+] score: 1
Further, deep sequencing detected let-7, miR-21, and miR-30 in rainbow trout eggs, which indicated that these miRNAs may play roles in controlling egg quality [21]. [score:1]
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22
[+] score: 1
Other miRNAs from this paper: gga-mir-30d, gga-mir-30a, gga-mir-30c-2, gga-mir-30e, gga-mir-30c-1
Sox14 specific oligonucleotides and a scrambled sequence were subsequently cloned in the second miRNA site of vector pRFPRNAi to generate synthetic miRNA30-like hairpins under the control of the chick U6 snRNA promoter, as described in [25]. [score:1]
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