sort by

51 publications mentioning zma-MIR156e

Open access articles that are associated with the species Zea mays and mention the gene name MIR156e. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 233
If miR156 overexpression was tied to developmental or environmental cues via conditional expression, then it could further optimize the use of miR156 overexpression as a bioconfinement tool. [score:8]
SPL downregulation results in altered plant phenotypeThe trend in overexpression of miR156 in field-grown plants was consistent with that of previous greenhouse and field studies, as was the inverse relationship between miR156 and SPL gene target abundance (Fig.   6, Additional file 1: Figure S1) [36, 37]. [score:8]
A transcriptomic study of the field-grown plants was performed to assess the influence of downstream genes impacted by miR156 expression, as well as any potential off-target effects, which are important for designing next-generation transgenic plants to further fine-tune the spatio-temporal expression of miR156 in switchgrass. [score:7]
P = 0.0103 The expression levels of four SPL genes (PvSPL1, PvSPL2, PvSPL3, and PvSPL6) were also examined using quantitative RT-PCR to determine the effects of miR156 overexpression on its target genes in field-grown plants. [score:7]
P = 0.0103 The expression levels of four SPL genes (PvSPL1, PvSPL2, PvSPL3, and PvSPL6) were also examined using quantitative RT-PCR to determine the effects of miR156 overexpression on its target genes in field-grown plants. [score:7]
The five miR156 SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) target genes had suppressed expression in one or more of the transgenic lines. [score:7]
miR156 expression was normalized using miR390 expression, and switchgrass PvUbq1 transcript abundance was used for normalization of data from each target gene with appropriate primers [36]. [score:6]
Six SPL gene annotations were downregulated in T37, which had the second highest miR156 overexpression. [score:6]
For the highest miR156 overexpression line T27, all eight SPL gene annotations were significantly downregulated (Additional file 2: Table S1). [score:6]
Line T27, which had the highest miR156 overexpression, showed significant downregulation for all five SPL genes. [score:6]
This outcome is the result of the downregulation of important miR156 SPL gene targets including SPL2/10 and SPL3/4/5. [score:6]
SPL downregulation was negatively associated with mature miR156 overexpression (Fig.   6, Additional file 1: Figure S3). [score:6]
Microarray analysis was performed to determine downstream gene expression effects of miR156 overexpression. [score:5]
miR156 targets the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor family, which is involved in many plant developmental processes including the vegetative to reproductive phase developmental transition [32– 35, 47]. [score:5]
The trend in overexpression of miR156 in field-grown plants was consistent with that of previous greenhouse and field studies, as was the inverse relationship between miR156 and SPL gene target abundance (Fig.   6, Additional file 1: Figure S1) [36, 37]. [score:5]
Medium overexpression lines (T27 and T37) produced a high number of tillers, which is a common occurrence in plants overexpressing miR156 [36, 37, 48, 50, 51]. [score:5]
Some transgenic events of switchgrass engineered to overexpress maize Corngrass1, a gene in the miR156 class of miRNAs, did not flower in a one-season California field trial, and weak overexpression levels did not affect biomass production [50]. [score:5]
Line T27 had the highest miR156 expression and showed the lowest PvSPL expression in general (Additional file 1: Figure S3). [score:5]
5 ± 0.4 [a] 34.75 ± 6.30 [a] T1460.6 ± 3.0 [c] 61.46 ± 0.91 [c] 24.6 ± 0.7 [c] 15.25 ± 1.93 [b] T3598.8 ± 4.7 [a] 68.01 ± 0.78 [b] 25.8 ± 0.5 [bc] 19.25 ± 3.33 [b] T270.0 ± 0.0 [d] N/a N/a N/a T3778.9 ± 7.5 [b] 40.78 ± 1.26 [d] 26.4 ± 1.0 [ab] 18.25 ± 2.06 [b] Lines T14 and T35 have low overexpression of miR156, whereas lines T27 and T37 have moderate levels of overexpression of the transgeneValues represent averages ± standard error. [score:5]
Transgenic switchgrass that overexpressed a rice miR156 precursor produced no flowering lines when grown in the greenhouse, and the low and medium overexpression lines produced more biomass than the control [36]. [score:5]
The expression level of miR156 was normalized using miR390 expression. [score:5]
Lines include the control (C), low miR156 overexpression lines (T14 and T35), and medium miR156 overexpression lines (T27 and T37). [score:5]
This two-year field study of miR156 -overexpressing transgenic switchgrass is the first field experiment in the eastern U. S. in which USDA-APHIS-BRS regulators allowed open flowering. [score:4]
We propose that induced miR156 expression could be further developed as a transgenic switchgrass bioconfinement tool to enable eventual commercialization. [score:3]
miR156 expression was positively associated with decreased and delayed flowering in switchgrass. [score:3]
Fig.  1 Complete randomized field design for open-flowering miR156 -overexpressing transgenic switchgrass in Oliver Springs, TN, USA. [score:3]
using the same miR156 -overexpressing plants [37]. [score:3]
Thus, the present study was the first opportunity to closely examine the dynamics of switchgrass reproduction in the field using transgenic lines with a range of a miR156 expression. [score:3]
Quantitative RT-PCR (qRT-PCR) analysis was used to assess transcript abundance of miR156 and its known target SPL genes. [score:3]
Specifically, miR156 targets the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factor family, which is involved in the transition from vegetative to reproductive phases [32– 35]. [score:3]
The miR156 target gene transcript abundance qRT-PCR analysis included PvSPL1, PvSPL2, PvSPL3, and PvSPL6. [score:3]
Overexpression of miR156 in switchgrass at low and moderate levels led to increased biomass and a non-flowering phenotype in the greenhouse [36]. [score:3]
Fig.  6Relative mature miR156 expression results from qRT-PCR. [score:3]
When Arabidopsis thaliana was engineered to constitutively express miR156, plants produced leaves that resembled juvenile leaves in size, shape, and trichome production [34]. [score:3]
Depending on its expression level, microRNA156 (miR156) can reduce, delay, or eliminate flowering, which may serve to decrease transgene flow. [score:3]
miR156 promotes the expression of juvenile leaf traits by repressing SPL genes involved in plant maturation, such as SPL2/10 and SPL3/4/5, all of which were reduced in T27 and T37 (Additional file 2: Table S1) [34, 49, 62, 63, 65, 66]. [score:3]
The overexpression of miR156 has been shown to delay flowering and increase biomass yield in multiple plant species [36, 48– 51]. [score:3]
A similar phenotype was seen in red clover (Trifolium pratense L. ) engineered to overexpress miR156; transgenic red clover plants had an increased number of shoots and delayed flowering [51]. [score:3]
In this unique field study of transgenic switchgrass that was permitted to flower, two low (T14 and T35) and two medium (T27 and T37) miR156 -overexpressing ‘Alamo’ lines with the transgene under the control of the constitutive maize (Zea mays) ubiquitin 1 promoter, along with nontransgenic control plants, were grown in eastern Tennessee over two seasons. [score:3]
Overexpression of miR156 in rice resulted in short panicles with reduced spikelet and grain number [64]. [score:3]
Arabidopsis thaliana plants engineered to overexpress miR156 had a moderate delay in flowering and an increase in total leaf number when grown under long days [48]. [score:3]
We found that medium overexpression of miR156, e. g., line T37, resulted in delayed and reduced flowering accompanied by high biomass production. [score:3]
It should also be noted that the overexpression of miR156 at moderate to high levels led to an increase in saccharification efficiency and reduction in lignin content [36, 37]. [score:3]
miR156 overexpression levels affect flowering timing and reproductive effort. [score:3]
A miR156 overexpression level that falls between that of line T27 and line T37 (Fig.   6) would be ideal as it would most likely result in a non-flowering, high-yielding line. [score:3]
On the contrary, line T35 had the lowest miR156 overexpression and had no significant change in any of the five SPL genes. [score:3]
Four transgenic and two nontransgenic parent ‘Alamo’ switchgrass lines were used to comparatively examine the phenotypic effects of miR156 overexpression (Additional file 1: Figure S1). [score:3]
Probes corresponding to other important genes involved in flowering pathways, such as Arabidopsis AtFT (Flowering Locus T)/rice OsFTL (Flowering Locus T- Like) genes, were also significantly affected in miR156 -overexpressing switchgrass (Additional file 2: Table S1). [score:3]
Our research objectives in this study were to deploy a range of miR156 -overexpressing switchgrass in a relevant field situation to closely examine flowering, reproduction, and biomass production. [score:3]
Because the ‘Alamo’ nontransgenic control flowered in the same period as in the past studies [54, 60], a delayed flowering phenotype observed in transgenic lines can be contributed to miR156 overexpression rather than environmental effects. [score:3]
We found that medium overexpression levels of miR156 such as those in line T37 resulted in delayed and reduced flowering accompanied by high biomass production. [score:3]
P ≤ 0.0001 Table 1 Flowering and reproduction of miR156 -overexpressing switchgrass and the nontransgenic control in the field Year Line Panicle number per plant Panicle length (cm) Spikelets per panicle Percent seed germination 2015 C29.0 ± 1.6 [a] 54.33 ± 1.69 [a] N/a4.75 ± 3.47 [b] T1422.2 ± 1.1 [b] 49.80 ± 1.29 [b] N/a5.50 ± 1.89 [b] T3522.6 ± 1.7 [b] 51.55 ± 1.67 [ab] N/a22.75 ± 3.97 [a] T270.0 ± 0.0 [d] N/a N/a N/a T379.9 ± 1.7 [c] 26.77 ± 2.07 [c] N/a0.25 ± 0.25 [b] 2016 C103.5 ± 4.0 [a] 73.34 ± 0.66 [a] 27. [score:3]
Medium overexpression lines (T27 and T37) show 10 and eight times increase, respectively, in mature miR156 levels compared to the control (Fig.   6). [score:2]
Lines categorized as low overexpressors (T14 and T35) had three and two times increase, respectively, in miR156 levels compared to control plants in the field. [score:2]
Baxter HL Mazarei M Dumitrache A Rodriguez M Natzke J Gou J Fu C Sykes RW Turner GB Davis MF Brown S Davison B Dixon RA Wang Z-Y Stewart CN Jr Transgenic miR156 switchgrass in the field: growth, recalcitrance and rust susceptibilityPlant Biotechnol J 2017 38. [score:1]
Fig.  5End-of-season dry biomass and height of miR156 transgenic switchgrass and control field grown in East Tennessee for 2 years. [score:1]
While Xie et al. [64] found no difference in seed fertility, all miR156 switchgrass transgenic lines had lower seed germination than the control in year two (Table  1). [score:1]
miR156 transcript abundance of field-grown plants was congruent with greenhouse results. [score:1]
Bioconfinement Floral transition miR156 Switchgrass Gene flow Switchgrass (Panicum virgatum L. ) is a native North American perennial prairie grass mostly known for its use as a biofuel feedstock. [score:1]
Fig.  2Time to first flower in the field for miR156 transgenic switchgrass lines and wild-type control. [score:1]
Genes related to the miR156 pathway and flowering were chosen for further examination. [score:1]
The level of mature miR156 transcript was examined using quantitative RT-PCR, and results were congruent with the results of the same clonal lines grown under greenhouse conditions [36] and in the field in which panicle removal was required [37]. [score:1]
[1 to 20 of 62 sentences]
2
[+] score: 108
For the sake of simplicity, we refer to 11 targets of miR156 and miR529 cooperative control as miR529 targets and we refer to the remaining 43 targets as miR156 targets. [score:9]
The differential regulation of SBP-box genes by two miRNA families provides an interesting example of the functions that these genes exhibit during land plant development; e. g., the low-level expression of SBP-box genes in an miR156-overexpression mutant prolonged the juvenile phase in maize [13] and Arabidopsis [14]. [score:7]
Meanwhile, a strong difference between Ka/Ks ratios for these two targets was also observed in Fig.   3. The miR156 targets had elevated Ka/Ks ratios, whereas miR529 targets had lower Ka/Ks ratios (Fig.   4c). [score:7]
Although we did not find evidence that miR156 and miR529 target all subgroup II-2 genes, the number of SBP-box genes targeted by miR156 is almost five times that of the number of genes cooperatively targeted by miR156 and miR529 (54 vs. [score:7]
Error bars indicate the standard error of the meanIn addition, our prediction suggested that 54 and 11 SBP-box genes in subgroup II-2 were separately targeted by the miR156 family and miR156/miR529 families, although not all SBP-box genes of subgroup II-2 were targeted by miR156 or the miR529 family. [score:5]
Among subgroup II-2 genes, miR156 targets evolve more rapidly than miR529 targets and experience comparatively relaxed purifying selection. [score:5]
Finally, our analyses led to the prediction of 54 SBP-box genes as the putative targets for miR156 and 11 SBP-box genes as the putative targets for miR529 (Fig.   1 and Additional file 4). [score:5]
Error bars indicate the standard error of the mean In addition, our prediction suggested that 54 and 11 SBP-box genes in subgroup II-2 were separately targeted by the miR156 family and miR156/miR529 families, although not all SBP-box genes of subgroup II-2 were targeted by miR156 or the miR529 family. [score:5]
Our previous work revealed that SBP-box genes targeted by miR156 evolve more rapidly and experience more relaxed purifying selection than genes targeted by both miR156 and miR529 [12]. [score:5]
Fig. 4Comparison of the mean Ks value (a), the mean Ka value (b) and the mean Ka/Ks ratios (c) for miR156 targets and miR529 targets. [score:5]
Therefore, we inferred that relaxed purifying selection might allow mutation at the miR156 binding sites and produce greater sequence diversity, which contributes to the increasing number of miR156 target genes. [score:4]
Our results indicated that miR156 targets had higher mean Ka and Ks values as compared to miR529 targets (Fig.   4a and b). [score:4]
Furthermore, one of our recent studies revealed that the miR156 family continually duplicates its gene copies, but retains conserved mature sequences, which would harmonize the regulation of increasing numbers of miR156 targets [23]. [score:4]
The genes marked by a single asterisk in the phylogenetic tree are regulated by miR156 and those marked by double asterisk are cooperatively regulated by both miR156 and miR529. [score:3]
In recent years, miR156/529 family members have been reported to target land plant SBP-box genes since these miRNA genes originated from land plants [12]. [score:3]
Prediction of miR156/miR529 target genes. [score:3]
Prediction of miR156/miR529 target genesApart from well-annotated genomes, these five species also have comprehensive miRNA information, in which miR156 and miR529 genes had been completely annotated using deep sequencing data. [score:3]
Taken together, the rapid expansion of subgroup II-2 genes and regulatory changes of miR156/529 on these genes could serve as new sources of functional diversity and confer phenotypic differences during development. [score:3]
However, we found that 11 SBP-box genes that are cooperatively controlled by miR156 and miR529 were in one subset of 54 miR156 putative targets. [score:3]
However, potential SBP-box targets controlled by miR156 and miR529 in these species were predicted when the miR529 genes from rice, maize and moss were used [23]. [score:3]
By contrast, subgroup II-2 genes evolve under relaxed purifying selection and have diversified through gene copy duplications and changes in miR156/529 regulation, which might contribute to morphological diversifications of land plants. [score:2]
For example, none of SBP-box genes were cooperatively regulated by miR156 and miR529 because there are no miR529 candidates found in core eudicots (i. e. Arabidopsis and poplar). [score:2]
Our previous results, together with evidence for a strong selective constraint against variations in binding sites cooperatively controlled by miR156 and miR529, provides evidence that the contraction of miR529 family members might lead to fewer SBP-box genes regulated by a combination of miR156/ miR529. [score:2]
Interestingly, subgroup II-1 genes have similar sequence and structural features to group I genes, whereas subgroup II-2 genes exhibit intrinsic differences on these features, including high copy numbers and the presence of miR156/ miR529 regulation. [score:2]
By contrast, subgroup II-2 genes experiencing comparatively relaxed purifying selection evolve more rapidly and have continually diversified through gene copy duplications and changes in miR156/529 regulation, which contributes to the morphological diversifications in land plants. [score:2]
All mature sequences of miR156 and miR529 genes were downloaded from miRBase release 21 [29]. [score:1]
They have characteristics of conserved genes: long protein sequences, a complex gene structure, lack of miR156/529 binding sites and nearly ubiquitous expression across different organs and tissues in distantly related plant species (Fig.   1, Table  1, and Additional file  1 and 2). [score:1]
Nevertheless, we found that some of subgroup II-2 SBP-box genes possessed a unique motif (motif 10), which is the responsive element of miR156 and miR529 (Additional file 1). [score:1]
We found that miR156/miR529 binding sites were present in subgroup II-2 SBP-box genes, but were not present in group I or subgroup II-1 genes (Additional file 1 and 4). [score:1]
Apart from well-annotated genomes, these five species also have comprehensive miRNA information, in which miR156 and miR529 genes had been completely annotated using deep sequencing data. [score:1]
[1 to 20 of 30 sentences]
3
[+] score: 99
While the majority of the grape SBP-box genes lacking a miR156/157 target site were expressed ubiquitously and constitutively, most genes bearing a miR156/157 target site exhibited distinct expression patterns, possibly due to the inhibitory role of the microRNA. [score:11]
Our experimental results, which were consistent with the expression patterns of miR156/157 -targeted genes in tomato and rice [21], [23], indicated that the miR156/157 -targeted grape SBP-box genes were generally expressed in a similar fashion to the VvmiR156f gene in all tissues tested (Fig. 6). [score:9]
Furthermore, the majority of these 12 grape SBP-box genes with miR156/157 target sites also exhibited the highest levels of expression in the early stages of fruit development, which gradually decreased or even vanished during the fruit ripening process. [score:6]
In general, the expression patterns of the 18 SBP-box genes could be classified into two types according to the presence or lack, of a miR156/157 target site (Fig. 6). [score:5]
In contrast to the grape SBP-box genes discussed above, Group 1 and Group 5 genes did not contain a miR156/157 target site and were all expressed ubiquitously and constitutively, with little or no variation in any of the tissues analyzed (Fig. 6). [score:5]
In the case of genes lacking a miR156/157 target site, including VvSBP4, VvSBP5, VvSBP6, VvSBP7, VvSBP14 and VvSBP17, there tended to be little or no variation in expression in any of the tissues tested. [score:5]
These results indicate that grape genes from these two groups may have functions that are distinct from the miR156/157 -targeted SBP-box genes in Groups 2, 3 and 4. Grape SBP-box Genes are Responsive to Abiotic and Biotic StressesTranscriptional control of stress-responsive genes is a crucial means by which plants respond to a range of abiotic and biotic stresses and research carried out in recent years has been productive in identifying transcription factors that are important for regulating these types of responses [60]. [score:4]
These results indicate that grape genes from these two groups may have functions that are distinct from the miR156/157 -targeted SBP-box genes in Groups 2, 3 and 4. Transcriptional control of stress-responsive genes is a crucial means by which plants respond to a range of abiotic and biotic stresses and research carried out in recent years has been productive in identifying transcription factors that are important for regulating these types of responses [60]. [score:4]
In contrast, genes containing a miR156/157 target site, including VvSBP1, VvSBP2, VvSBP3, VvSBP8, VvSBP9, VvSBP10, VvSBP11, VvSBP12, VvSBP13, VvSBP15, VvSBP16 and VvSBP18, were expressed at relatively higher levels in leaves, stems and tendrils compared to the reproductive tissues analyzed. [score:4]
Indeed, 12 of 18 grape SBP-box genes contained a miR156/157 target site in the V. vinifera genome (Fig. 1). [score:3]
The miR156/157 target sites are denoted by blue vertical lines. [score:3]
In rice, for instance, 11 of the 19 SBP-box genes have been revealed to be putative targets of OsmiR156 [21], while 10 of 15 SBP-box gene family members in tomato were found to carry putative miR156/ 157-response elements [23]. [score:3]
Interestingly, the miR156/157 -targeted SBP-like genes, including sequences from rice, Arabidopsis and grape, were distributed into only three of the subgroups (Groups 2, 3 and 4). [score:3]
Of the twelve grape SBP genes containing a miR156/157 target site, Group 4 members (with the exception of VvSBP4) bore this site within their 3′ UTRs (Fig. 3B), which is similar to AtSPL3, AtSPL4 and AtSPL5 in Arabidopsis. [score:3]
As a gene family encoding transcription factors, more than half of the SBP-box genes identified to date have been found to be targeted by miR156/ 157. [score:3]
To date, miR156/157 target sites were found in 10 Arabidopsis [57], 11 rice [21] and 10 tomato [23] SBP-box genes. [score:3]
In most cases, miR156/157-regulated SBP-box genes tend to play a role in the control of phase change and reproductive development [58], [59]. [score:3]
The three groups of SBP-box genes discussed above (Groups 2, 3, and 4), with the exception of SBP4, all contain a miR156/157 target site. [score:3]
Furthermore, both the locations of miR156/157 target sites and composition of encoded SBP domains were compared in each of the grape SBP genes to gain further insight into their evolutionary relationship with one another. [score:2]
59Schwab R (2012b) Roles of miR156 and miR172 in Reproductive Development. [score:2]
SBP-box genes from Arabidopsis, rice, tomato, and grape that contain complementary sequences for miR156/157 are marked with an asterisk. [score:1]
0059358.g001 Figure 1Alignment of miR156/157 complementary sequences within grape SBP genes. [score:1]
Chromosomal distribution of SBP and miR156 genes, as well as synteny of SBP-box genes in grape. [score:1]
Expansion Patterns and Distribution of Grape SBP and miR156 Genes in the Grape Genome. [score:1]
Expansion Patterns and Distribution of Grape SBP and miR156 Genes in the Grape GenomeAccording to available annotation information, the 18 grape SBP genes were dispersed on all grape chromosomes except for chromosomes 2, 3, 6, 9, 13 and 16. [score:1]
Alignment of miR156/157 complementary sequences within grape SBP genes. [score:1]
Although SBP-box genes have been identified in numerous plants including green algae, moss, silver birch, snapdragon, Arabidopsis, rice and maize, there is little information concerning SBP-box genes, or the corresponding miR156/157, function in grapevine. [score:1]
SBP-box genes that contained complementary sequences for miR156/157 are marked with an asterisk. [score:1]
Although grapevine (V. vinifera) is one of the most important perennial fruit crops worldwide, there is little information concerning SBP-box gene, or the corresponding miR156/157, function in this species [24]. [score:1]
SBP and miR156 genes are indicated by vertical orange and black lines, respectively. [score:1]
0059358.g004 Figure 4Chromosomal distribution of SBP and miR156 genes, as well as synteny of SBP-box genes in grape. [score:1]
In addition, in accordance with findings in other species, we also found that 12 of the 18 grape SBP genes identified in this study contained sequences that were complementary to miR156/157, with a maximum of one to three mismatches to the mature VvmiR156/157 sequences (Fig. 1). [score:1]
Previously, nine members of the miR156/157 family, termed VvmiR156a to VvmiR156i, which are highly conserved in plants and are thought to interact with numerous SBP-box genes, were identified in the V. vinifera genome [53]– [56]. [score:1]
This provides yet another example of the mutual relationship between miR156/157 and SBP-box genes. [score:1]
Eighteen SBP-box gene family members were identified in Vitis vinifera, twelve of which bore sequences that were complementary to miRNA156/157. [score:1]
[1 to 20 of 35 sentences]
4
[+] score: 67
miR156 target: GRMZM2G126018_T01 (SBP23); miR159 target: GRMZM2G139688_T01 (GA-MYB); miR164 target: GRMZM2G393433_T01 (CUC2); miR168 target: GRMZM2G039455_T01 (AGO1c). [score:9]
Although we only tested one SBP-like target of miR156, 11 out of 26 SBP-like maize genes were predicted as targets for this miRNA, all of them exhibiting the same target sequence as SBP23 (Supplementary Material, Table S2). [score:7]
On the other hand, increments of both, miRNA and target, upon hormone 50% reduction (e. g., miR156, miR164, miR168) suggest miRNA up-regulation might be required to control the levels of transcripts induced during SE. [score:6]
Therefore, although miR164 and miR156 targets might display contrasting behavior in undifferentiated tissues (darkness, hormones' presence) between maize genotypes, their expression regulation is apparently required for plant regeneration through SE, regardless the genotype (Figure 6). [score:6]
Conversely, these studies have identified novel targets for conserved miRNAs such as miR156 and miR164, or known targets with novel miRNA sites. [score:5]
Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3. [score:5]
In a previous study performed on VS-535-derived EC, we found that development-related miRNAs such as miR156, miR159, miR164 and miR168 decreased as the length of subculture increased, while stress-related miRNAs such as miR397, miR398, miR408, and miR528 remained highly expressed (Dinkova and Alejandri-Ramirez, 2014). [score:4]
Targeting of SBP-like transcripts by miR156 is highly conserved in plants (Rhoades et al., 2002; Xie et al., 2006; Gandikota et al., 2007) and has been reported as relevant for vegetative to reproductive phase changes and plastochron length (Wu and Poethig, 2006; Wang et al., 2008). [score:3]
The miR156 -mediated suppression of SBP transcripts is probably required for early SE, as demonstrated for Arabidopsis zygotic embryogenesis (Nodine and Bartel, 2010). [score:3]
The Squamosa Promoter Binding protein (SBP)-like transcript GRMZM2G126018_T01 (SBP23) targeted by miR156 showed significantly higher levels upon hormone half reduction under darkness than in the presence of light (Figure 3, b–c vs. [score:3]
For maize, it has been shown that miR156 gene family overexpression renders plants with increased number of leaves and delayed flowering (Chuck et al., 2007). [score:3]
Dual effects of miR156 -targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana. [score:3]
Genomic organization, differential expression, and interaction of SQUAMOSA promoter -binding-like transcription factors and microRNA156 in rice. [score:2]
Similar to miR156 and SBP23, there was poor correlation between miR168 and AGO1c transcript levels. [score:1]
The levels of miR156 and miR164 decreased by three-fold (with respect to 50% hormones) for VS-535, but showed no change for H-565 (Figure 2, lanes c vs. [score:1]
Previous studies in maize long-term subcultured EC indicated that miR156, miR159, miR164, miR168, and miR319 importantly reduce their levels in subcultures maintained for more than 18 months (Dinkova and Alejandri-Ramirez, 2014). [score:1]
However, in maize EC subcultured for long periods (up-to 2 years) we have found a gradual reduction in miR156, miR164 and miR168 levels without impairment on the callus embryogenic potential (Dinkova and Alejandri-Ramirez, 2014). [score:1]
For instance, at least two-fold increase in miR156, miR164, miR168 and miR408, was observed in VS-535, 50% hormones, while a modest 1.2-1.4-fold increase under the same conditions was evident for H-565 (Figure 2 and Supplementary Material, Figure S1). [score:1]
Under darkness, SBP23 inversely mirrored miR156 changes due to hormone depletion (Figure 3A, lanes b–c; and Figure 3B, lanes a–b). [score:1]
miR156 and miR164 have been found as SE-abundant miRNAs in several species, including maize (Li et al., 2012; Shen et al., 2013; Dinkova and Alejandri-Ramirez, 2014; Wu et al., 2015). [score:1]
They identified miR528, miR156, miR166, miR168, miR390, miR164, miR167, miR398, miR397, miR408, and miR319 as the most abundant during dedifferentiation. [score:1]
[1 to 20 of 21 sentences]
5
[+] score: 64
Other miRNAs from this paper: zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR162, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR394a, zma-MIR394b, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR399a, zma-MIR399c, zma-MIR399b, zma-MIR399d, zma-MIR399e, zma-MIR399f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR319a, zma-MIR319c, zma-MIR319b, zma-MIR319d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR393a, zma-MIR408a, zma-MIR156k, zma-MIR160f, zma-MIR2118a, zma-MIR2118b, zma-MIR2118c, zma-MIR2118d, zma-MIR2118e, zma-MIR2118f, zma-MIR2118g, zma-MIR2275a, zma-MIR2275b, zma-MIR2275c, zma-MIR2275d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR397a, zma-MIR397b, zma-MIR398a, zma-MIR398b, zma-MIR399g, zma-MIR399h, zma-MIR399i, zma-MIR399j, zma-MIR408b, zma-MIR482, zma-MIR528a, zma-MIR528b, zma-MIR529, zma-MIR827, zma-MIR1432, zma-MIR444a, zma-MIR444b
Moreover, targets of miR156 and miR172 exert positive feedback on the expression of miRNA genes that suppress themselves [61]. [score:7]
For example, SPL13 (SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 13) is the miR156 target gene and down-regulation of SPL13 by miR156 appears to be essential for the transition to the vegetative-leaf stages. [score:6]
These 17 targets were targets of 11 miRNA families (miR156, miR164, miR166, miR167, miR168, miR169, miR319, miR393, miR408, miR528 and zma-miRn6). [score:5]
In maize, Corngrass1 (Cg1) mutants overexpress two tandem miR156 genes [23], which target teosinte glume architecture1 (tga1). [score:5]
A-K represented the expression profiles of some predicted target genes of miR156, miR164, miR166, miR167, miR168, miR169, miR319, miR393, miR408, miR528 and zma-miRn6 in dry and imbibed seeds, respectively. [score:5]
The 12 down-regulated miRNA families were miR156, miR159, miR164, miR166, miR167, miR168, miR169, miR172, miR319, miR393, miR394 and miR397. [score:4]
Whereas miR156 and miR166, although they both were down-regulated in imbibed seed only by 1.5 folds, their basal frequencies in dry seeds were 525,736 and 432,679, respectively, much higher than those of miR159, miR393 and miR394. [score:4]
Even they were significantly down-regulated in the imbibed seeds; there still were 342,738 and 282021 miRNA transcripts of miR156 and miR166, respectively, in the imbibed seeds. [score:4]
Overexpression of miR156 in Arabidopsis, rice and maize, repressed the transcript abundance of related SPL genes and reduced apical dominance, delayed flowering time, caused dwarfism and increased total leaf numbers and biomass [58], [57], [23]. [score:3]
Teosinte glume architecture 1 (tga1), which plays an important role in maize domestication, has been identified as a target of miR156 [25]. [score:3]
Teosinte glume architecture1 (tga1), which encodes an SBP-domain family protein as does Arabidopsis SPL13 and plays an important role in maize domestication, has been identified as a target of miR156 [25]. [score:3]
MiR156 plays crucial role in the control of juvenile-to-adult transition in plant by targeting the SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) plant-specific transcription factors [56]. [score:2]
Previous studies showed that miRNA gene regulation cascades exist and the miR156 pathway acts upstream of the miR172 pathway. [score:2]
The balance between miR156 and SPL appears to be important to maintain proper plant development and phase transition. [score:2]
Mutated SPL13 that is resistant to miR156 over-accumulates at the post germination stages and causes a delay in vegetative leaf development from the cotyledon-stage seedlings [14], [15]. [score:2]
In Arabidopsis thaliana, there are 17 members of the SPL family of transcription factors, and 11 of them are the MiR156 targets [57]. [score:2]
To validate conserved miRNAs identified and novel ones predicted, quantitative RT-PCR (qRT-PCR) was performed on 10 randomly selected miRNAs, miR156, miR159, miR166, miR167, miR319, miR408, miR528, zma-miRn6, zma-miRn15 and zma-miRn37 in dry and imbibed seed. [score:1]
The largest miRNA family size identified was miR166 that consisted of 14 members and miR156/157, miR167 and miR169 possessed 12, 10 and 9 members, respectively; whereas miR162, miR529, miR827 and miR1432 had only one member detected in the maize seeds (Figure 4). [score:1]
The most abundant families were miR156/miR157, miR166, miR168 and miR528, and the least abundant families were miR162 and miR399 (Figure 3). [score:1]
Not only the miRNA156 and miRNA166 families were abundant in dry and imbibed seeds, but also they had more family members than any other miRNA families, suggesting the importance of these two miRNA families in dry and imbibed seeds. [score:1]
A decline in miR156 abundance provides a permissive environment for flowering and is paralleled by a rise in SPL levels [60]. [score:1]
[1 to 20 of 21 sentences]
6
[+] score: 46
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR171a, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR160e, osa-MIR160f, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR171b, osa-MIR171c, osa-MIR171d, osa-MIR171e, osa-MIR171f, osa-MIR171g, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR171h, osa-MIR408, osa-MIR172d, osa-MIR171i, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR164f, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, osa-MIR390, osa-MIR444a, zma-MIR171d, zma-MIR171f, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR408a, zma-MIR156k, zma-MIR160f, osa-MIR528, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR1432, osa-MIR827, osa-MIR2118a, osa-MIR2118b, osa-MIR2118c, osa-MIR2118d, osa-MIR2118e, osa-MIR2118f, osa-MIR2118g, osa-MIR2118h, osa-MIR2118i, osa-MIR2118j, osa-MIR2118k, osa-MIR2118l, osa-MIR2118m, osa-MIR2118n, osa-MIR2118o, osa-MIR2118p, osa-MIR2118q, osa-MIR2118r, osa-MIR2275a, osa-MIR2275b, zma-MIR2118a, zma-MIR2118b, zma-MIR2118c, zma-MIR2118d, zma-MIR2118e, zma-MIR2118f, zma-MIR2118g, zma-MIR2275a, zma-MIR2275b, zma-MIR2275c, zma-MIR2275d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR390a, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR408b, zma-MIR528a, zma-MIR528b, zma-MIR827, zma-MIR1432, zma-MIR390b, osa-MIR395x, osa-MIR395y, osa-MIR2275c, osa-MIR2275d, zma-MIR444a, osa-MIR6251
[58, 67, 68], most of them showed different expression patterns upon exposure to light (Additional file 14) except (a) miR156, miR166, miR172, which showed almost identical expression curves, and (b) miR171 and miR390, which showed shifted expression patterns. [score:7]
Though miR156, regulators of flowering time, phase changing modulation, later embryonic maturation and root development [76], together with miR168, regulators of stress responses and signal transductions in plant development [67], showed very similar expression patterns between maize and rice during de-etiolation (Additional file 14). [score:7]
For those miRNAs that showed similar expression patterns between maize and rice, i. e., miR156, miR166, miR168, miR172, miR2275 and miR528, GO enrichment analysis of their predicted targets was applied (Additional file 13). [score:5]
Xie K, Shen J, Hou X, Yao J, Li X, Xiao J, et al. Gradual increase of miR156 regulates temporal expression changes of numerous genes during leaf development in rice. [score:5]
Analysis showed that two target genes of miR156 (GRMZM2G097275 and GRMZM2G126018) were reported to be putative positive Kranz regulators [47]. [score:4]
Based on our result, two putative positive Kranz regulators reported by Wang et al. (2010) were predicted as potential targets of miR156. [score:4]
Our results showed that two target genes of miR156 (GRMZM2G097275 and GRMZM2G126018) were reported to be putative positive Kranz regulators (Additional file 15). [score:4]
GO enrichment analysis for targets of miR156 in maize and rice Additional file 17. [score:3]
Considering that miR156 is a highly conserved miRNA across plant species [76], we conducted GO enrichment anlaysis of all the predicted 24 and 21 target genes for maize and rice miR156 respectively. [score:3]
miR156, miR160, miR164, miR166, miR167, miR171, miR172, and miR390, had been earlier reported to play evolutionarily conserved roles in plant development [54]. [score:2]
Among these 8 miRNAs, miR156, miR172 and miR408 are conserved miRNA families between maize and rice. [score:1]
Many of them, i. e., miR156, miR160, miR164, miR166, miR167, miR171, miR172 and miR390, were suggested to play highly evolutionary conserved roles across plant species [54]. [score:1]
[1 to 20 of 12 sentences]
7
[+] score: 45
One of the most well understood examples is the antagonistic interplay of miR156 and miR172 node, that is, SPL9, the down-regulated target of miR156, is able to directly bind to the promoter of miR172b gene and correspondingly promote its expression, which coordinately regulated the transition from vegetative phase to reproductive phase. [score:10]
Unlike Zma-miR156, Zma-miR166 family members exhibited similar expression patterns, but their dynamic expression status was likely the results of the transient expression of Zma-miR166a/b/f in kernel stages (Figure 2C). [score:7]
For example, the reads abundance and expression trends of Zma-miR156 family are mainly contributed by the member of Zma-miR156a, which peaks at 7 DAP and then decreases gradually at later stages, whereas Zma-miR156k and Zma-miR156j have low and moderate expression levels, respectively, and the latter is expressed during all kernel and endosperm stages with no obvious variation (Figure 2B). [score:7]
For example, miR156 reaches its peak expression at 7 DAP, while miR172 exhibits its lowest expression level at the same developmental stage (Figure S2A). [score:6]
In contrast, the expression level of Zma-miR156 was significantly higher in the endosperm stages than in the kernel stages. [score:3]
” Correspondingly, the abundance of miR156 decreases at 10 and15 DAP while miR172 increases, consistent with the expression trends during leaf aging. [score:3]
Figure S2 The expression pattern of miR156 and miR172 (A) as well as miR390 and TAS3 (B) in 0-, 3-, 5-DAP kernels and 7-, 10-, and 15-DAP endosperm in maize. [score:3]
Specifically, increased abundance of miR156 prolongs the juvenile stage and delays the adult stage transition, while highly expressed miR172 leads to early flowering and premature features of vegetative growth. [score:3]
Among them, Zma-miR168, Zma-miR166, Zma-miR156, Zma-miR528, Zma-miR827, and Zma-miR167 were the top six most abundantly expressed miRNAs in both reciprocal crosses, corresponding to more than 98% of the total number of known miRNAs (Table S2, Figure 2A). [score:3]
[1 to 20 of 9 sentences]
8
[+] score: 44
Other miRNAs from this paper: zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR160e, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR396b, zma-MIR396a, zma-MIR399a, zma-MIR399c, zma-MIR399b, zma-MIR399d, zma-MIR399e, zma-MIR399f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR393a, zma-MIR156k, zma-MIR160f, zma-MIR396c, zma-MIR396d, zma-MIR2118a, zma-MIR2118b, zma-MIR2118c, zma-MIR2118d, zma-MIR2118e, zma-MIR2118f, zma-MIR2118g, zma-MIR2275a, zma-MIR2275b, zma-MIR2275c, zma-MIR2275d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR390a, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR396e, zma-MIR396f, zma-MIR396g, zma-MIR396h, zma-MIR399g, zma-MIR399h, zma-MIR399i, zma-MIR399j, zma-MIR529, zma-MIR390b
Conversely, in V-372, members of 7 families—zma-miR164, zma-miR169, zma-miR393, zma-miR396, zma-miR399, zma-miR529, and zma-miR2275—were significantly up-regulated; and zma-miR156, zma-miR159, zma-miR166 and zma-miR395 families were significantly down-regulated. [score:7]
Family specific target mRNAs miRNA family miRNA Target mRNAs Annotations miR156 zma-miR156-a,c,d,e,f,g,h,I,j,k,l gnl|GNOMON|13750094. [score:5]
Similarly, miR156 is reported to target the expression of tga1 (Teosinte glume architecture 1) (Chuck et al., 2007). [score:5]
For example, the miR156 family regulates the expression of SPL, leading to plant developmental phase transitions (Wang et al., 2009; Chen et al., 2010) (Figure 6). [score:5]
However, zma-miR156 and zma-miR159 were significantly down-regulated. [score:4]
Similar to observations for miR156 family, Type II (down-regulated) interactions were found for SPL genes in both genotypes. [score:4]
Li et al. (2013) reported the up-regulation of miR156 at the early stage of drought stress in the maize seedlings. [score:4]
Over -expression of miR156 encoding the maize Cg1 gene showed to prevent the flowering, and improved the digestibility and starch content in switchgrass (Chuck et al., 2011). [score:3]
The regulation of SPL TFs through miR156 was also reported during somatic embryogenesis of maize (Chávez-Hernández et al., 2015). [score:2]
miR156-regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana. [score:2]
m tpa: squamosa promoter -binding (sbp domain) transcription factor family protein zma-miR156-a,c,d,e,f,g,h,I,j,k,l gnl|GNOMON|66064033. [score:1]
The miRNA families with the largest numbers of members included zma-miR166, zma-miR395 and zma-miR156, with 14, 14, and 12 members, respectively. [score:1]
Additionally, Liu et al. (2014) reported that miR156 controlled several SPL genes during the juvenile-to-adult phase transition in maize. [score:1]
[1 to 20 of 13 sentences]
9
[+] score: 41
Other miRNAs from this paper: zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR162, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR394a, zma-MIR394b, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR396b, zma-MIR396a, zma-MIR399a, zma-MIR399c, zma-MIR399b, zma-MIR399d, zma-MIR399e, zma-MIR399f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR319a, zma-MIR319c, zma-MIR319b, zma-MIR319d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR393a, zma-MIR408a, zma-MIR156k, zma-MIR160f, zma-MIR396c, zma-MIR396d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR396e, zma-MIR396f, zma-MIR396g, zma-MIR396h, zma-MIR397a, zma-MIR397b, zma-MIR398a, zma-MIR398b, zma-MIR399g, zma-MIR399h, zma-MIR399i, zma-MIR399j, zma-MIR408b, zma-MIR482, zma-MIR528a, zma-MIR528b, zma-MIR529, zma-MIR827, zma-MIR1432, zma-MIR444a, zma-MIR444b
In our data, we found that the expression of miR156 and miR172 families are anti-correlated (Table S4); miR156 is expressed higher in young roots and seedlings but lower in adult tissues (tassel, ear, and pollen), while miR172 expression has an opposite trend, albeit with a lower overall expression level. [score:9]
For example, miR156 targets SBP transcription factors [80], [81], while miR159 targets the MYB family [82]. [score:5]
Intriguingly, mutations in Corngrass1 (Cg1) result in the over -expression of miR156 and decreased miR172 levels, resulting in alterations of the juvenile to adult phase transition [24], [25]. [score:4]
For instance, teosinte glume architecture 1 (tga1) is one of the major genes responsible for the evolution of maize from its ancestor teosinte and has also been identified as a target for miR156 [24], [25]. [score:3]
We note that the mature miRNA products of families miR529 and miR156 are identical from positions 8–14; which are key residues in miRNA-target recognition. [score:3]
In contrast, miR156, miR159, miR167, miR168, miR169, miR171, miR319, and miR529 had high expression counts (slightly over 3,000 RPM, on average). [score:3]
It is likely that miR529 is either related to, or is a sub-grouping of, the miR156 family, but with a distinct tissue expression profile. [score:3]
The miR156, miR164, miR168, miR393, miR395, miR396, miR398, and miR399 families had higher signatures in juvenile root and seedling tissues while miR172 demonstrated a higher expression level in reproductive tissues (tassel and ear). [score:3]
Both miR156 and miR529 were predicted to target genes encoding the SBP box. [score:3]
This converse regulatory relationship between miR156 and miR172 has also been reported recently [62]. [score:2]
These results fit well with the current mo dels of phase transition, whereby opposing gradients of miR156 and miR172 are responsible for the transition from juvenile to adult. [score:1]
However, a subset of these was found as unusually compact clusters, with less than 2000nt separating adjacent genes, as shown in Table 2. Two members of the miRNA156 family (miR156b and miR156c) on chromosome 3 are separated by less than 200nt, as are two members of the miRNA166 family on chromosome 5. The close distance of miR156b and miR156c is observed in several monocots and they are transcribed as one transcript (polycistronic) in maize [24] and rice [35]. [score:1]
These families are: miR156, miR160, miR164, miR166, miR167, miR172, miR396, and miR528. [score:1]
[1 to 20 of 13 sentences]
10
[+] score: 33
Other miRNAs from this paper: ath-MIR156a, ath-MIR156b, ath-MIR156c, ath-MIR156d, ath-MIR156e, ath-MIR156f, ath-MIR159a, ath-MIR160a, ath-MIR160b, ath-MIR160c, ath-MIR162a, ath-MIR162b, ath-MIR164a, ath-MIR164b, ath-MIR166a, ath-MIR166b, ath-MIR166c, ath-MIR166d, ath-MIR166e, ath-MIR166f, ath-MIR166g, ath-MIR167a, ath-MIR167b, ath-MIR168a, ath-MIR168b, ath-MIR169a, ath-MIR172a, ath-MIR172b, ath-MIR159b, osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR162a, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, ath-MIR167d, ath-MIR169b, ath-MIR169c, ath-MIR169d, ath-MIR169e, ath-MIR169f, ath-MIR169g, ath-MIR169h, ath-MIR169i, ath-MIR169j, ath-MIR169k, ath-MIR169l, ath-MIR169m, ath-MIR169n, ath-MIR172c, ath-MIR172d, ath-MIR395a, ath-MIR395b, ath-MIR395c, ath-MIR395d, ath-MIR395e, ath-MIR395f, ath-MIR396a, ath-MIR396b, ath-MIR399a, ath-MIR399b, ath-MIR399c, ath-MIR399d, ath-MIR399e, ath-MIR399f, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR396a, osa-MIR396b, osa-MIR396c, osa-MIR399a, osa-MIR399b, osa-MIR399c, osa-MIR399d, osa-MIR399e, osa-MIR399f, osa-MIR399g, osa-MIR399h, osa-MIR399i, osa-MIR399j, osa-MIR399k, ath-MIR408, ath-MIR156g, ath-MIR156h, ath-MIR159c, ath-MIR164c, ath-MIR167c, ath-MIR172e, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR160e, osa-MIR160f, osa-MIR162b, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR171h, osa-MIR408, osa-MIR172d, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR164f, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR162, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, osa-MIR396e, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR396b, zma-MIR396a, zma-MIR399a, zma-MIR399c, zma-MIR399b, zma-MIR399d, zma-MIR399e, zma-MIR399f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171h, zma-MIR408a, zma-MIR156k, zma-MIR160f, osa-MIR529a, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR529b, osa-MIR169r, osa-MIR396f, zma-MIR396c, zma-MIR396d, osa-MIR2118a, osa-MIR2118b, osa-MIR2118c, osa-MIR2118d, osa-MIR2118e, osa-MIR2118f, osa-MIR2118g, osa-MIR2118h, osa-MIR2118i, osa-MIR2118j, osa-MIR2118k, osa-MIR2118l, osa-MIR2118m, osa-MIR2118n, osa-MIR2118o, osa-MIR2118p, osa-MIR2118q, osa-MIR2118r, osa-MIR2275a, osa-MIR2275b, zma-MIR2118a, zma-MIR2118b, zma-MIR2118c, zma-MIR2118d, zma-MIR2118e, zma-MIR2118f, zma-MIR2118g, zma-MIR2275a, zma-MIR2275b, zma-MIR2275c, zma-MIR2275d, osa-MIR396g, osa-MIR396h, osa-MIR396d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR396e, zma-MIR396f, zma-MIR396g, zma-MIR396h, zma-MIR399g, zma-MIR399h, zma-MIR399i, zma-MIR399j, zma-MIR408b, zma-MIR529, osa-MIR395x, osa-MIR395y, osa-MIR2275c, osa-MIR2275d, ath-MIR156i, ath-MIR156j
Beyond miR156 and miR172, miR164 targets genes encoding NAM proteins, and may be involved in regulating ear development (Table  3), similar to how miR164 is postulated to regulate NAC-domain targets in Arabidopsis [58]. [score:8]
Interestingly, some target transcripts were regulated by pairs of miRNAs: both miR156 and miR529 targeted five members of the same SBP family, and the miR159/319 pair regulated three MYB domain transcription factors. [score:7]
zma-miR156 targeted 13 unique genes including SPL genes and zma-miR529 targeted 18 unique genes including ZCN19 (a possible maize FT ortholog) (Table  3), indicating that these two families might play key roles in ear development [31, 54]. [score:6]
The six most abundantly expressed miRNA families were miR166, miR168, miR167, miR156, miR159, and miRs6. [score:3]
Among the conserved miRNA families, zma-miR156 and zma-miR529 had the highest number of gene targets. [score:3]
Previous studies showed that miR156 and miR172 function throughout flower development from the earliest stages (floral induction, stage I) to very late stages (floral organ cell-type specification, stage IV) [31- 34]. [score:2]
Figure 4 miR156 and miR172 in maize flower development (Adapted from Poethig (2009). [score:2]
Of these, 45 miRNAs aligned with 59 members of 21 maize miRNA families, while the others corresponded to members of miRNA families from three other plant species, including rice (osa-miR156/162/164/168/396/529) Arabidopsis (ath-miR156/164/167) and sorghum (sbi-miR396). [score:1]
The levels of miR156 and miR172 are conflicting during phase transition (Figure  4b). [score:1]
[1 to 20 of 9 sentences]
11
[+] score: 30
Other miRNAs from this paper: zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR396b, zma-MIR396a, zma-MIR399a, zma-MIR399c, zma-MIR399b, zma-MIR399d, zma-MIR399e, zma-MIR399f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR319a, zma-MIR319c, zma-MIR319b, zma-MIR319d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR408a, zma-MIR156k, zma-MIR160f, zma-MIR396c, zma-MIR396d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR396e, zma-MIR396f, zma-MIR396g, zma-MIR396h, zma-MIR399g, zma-MIR399h, zma-MIR399i, zma-MIR399j, zma-MIR408b, zma-MIR1432
In the second group of miRNAs, the expression of five miRNA families (miR156, miR158, miR160, miR395, and miR8677) was down-regulated in response to RBSDV infection in maize. [score:6]
The regulation of miR156 expression is apparently related to the improvement of plant architecture in rice (Chen et al., 2015), Solanum tuberosum ssp. [score:4]
The results of the present study showed that down-regulation of miR156, miR158, miR160, and miR395 upon RSBVD infection could be related to plant architecture in maize. [score:4]
Three other miRNAs (miR156e, miR169i-p5, and miR396a-5p) were down-regulated (Fig. 2). [score:4]
Only one target gene each was identified for miR156e and miR396a-5p, namely a SQUAMOSA promoter -binding (SPB) protein and a growth -regulating factor, respectively (Supplementary Table S5). [score:4]
In rice infected with RBSDV at later stages, the expression of miR156 increased in leaves and roots (Sun et al., 2014). [score:3]
A total of 99 transcripts from 48 genes were identified for 10 known miRNAs (miR156e, miR169i-p5, miR169l-5p, miR319b-p3, miR319b-p3-1, miR319b-p3-Pt, miR396a-5p, miR408a, miR4366-p3, and miR8155) that exhibited at least two-fold change in expression and that had at least 10 reads per dataset. [score:3]
Wang JW 2014 Regulation of flowering time by the miR156 -mediated age pathway. [score:2]
[1 to 20 of 8 sentences]
12
[+] score: 29
miRNA156, 393, 396 and 397, and their respective targets, may contribute to the maize grain filling rate by regulating maize growth, development and environment stress response (Fig 5). [score:5]
Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3. [score:5]
A point mutation within the miR156 target regin of OsSPL14 in rice generates the plant with a reduced tiller number, increased lodging resistance and enhanced grain yield [30]. [score:4]
We also detected the expression pattern of SPL, and found that SPL reduced linearly with the increase of zma-miR156 (Fig 4). [score:3]
Ten out of the 16 SPL family members have been predicted to be miR156 targets [9]. [score:3]
As another target gene of miR156, OsSPL16 encodes a protein that promotes cell division, also with positive consequences for grain width and yield in rice [32]. [score:3]
For example, the Squamosa promoter binding protein-like (SPL) familyis considered to be the target gene of zma-miR156. [score:2]
In the present study, zma-miR156 increased linearly from 17 to 28 DAP, this is consistent with the result in the seed development process of rice [33], wheat [34] and barley [35]. [score:2]
The structure of pre-miRNA156, 169, 393, 396 and 397.. [score:1]
0125800.g002 Fig 2 Six novel maize miRNAs (miRt4, miRt13, miRt15, miRt17 and miRt21, miRt28) and six conserved miRNAs (miR156, miR162, miR172, miR393, miR396 and miR408) were chosen to verify the sequencing results via the qRT-PCR analysis. [score:1]
[1 to 20 of 10 sentences]
13
[+] score: 29
Moreover, two targets of miR156 were found to positively regulate miR172 expression by binding their sequence to the regulatory region of miR172 (Wu et al., 2009). [score:7]
In our study, expression of miR172 was down regulated, while that of miR156 was up regulated in IFC compared to CFC ovaries (Table 2), suggesting a similar interaction between miR172 and miR156 may exist in maize ovary as well. [score:4]
Gibberellin (GA) is thought to play diverse roles in plant growth and development, including flowering time, with overexpression of miR156 reducing GA responses during flowering (Yu et al., 2012). [score:4]
Gibberellin regulates the Arabidopsis floral transition through miR156 -targeted SQUAMOSA promoter binding–like transcription factors. [score:4]
microRNA156 -targeted SPL/SBP box transcription factors regulate tomato ovary and fruit development. [score:4]
It was also revealed that miR156 controls the initial steps of fleshy fruit development in tomato, playing an important role in ovary and fruit development (Ferreira et al., 2014). [score:3]
The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis. [score:3]
[1 to 20 of 7 sentences]
14
[+] score: 25
The expression of the two miRNA families is negatively correlated, that is, miR156 expression is higher in younger tissues while miR172 expression is higher in adult tissues [73]. [score:7]
Expression of specific members of the miR156 gene family is repressed by a developmental regulation factor produced in leaf primordia [75]. [score:5]
74Wu G, Poethig R (2006) Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3. [score:5]
miR156 (Table 1), as a juvenile gene, regulates the transition from the juvenile phase to the adult phase through repression of SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) gene expression [74]. [score:4]
There are two key miRNA gene families in this pathway, namely miR156 for suppression of and miR172 for promotion of the phase change [71], [72]. [score:3]
75Yang L, Conway S, Poethig R (2011) Vegetative phase change is mediated by a leaf-derived signal that represses the transcription of miR156. [score:1]
[1 to 20 of 6 sentences]
15
[+] score: 18
For instance, each member of miR156 targeted eight genes and all 12 members of miR156 regulated the target GRMZM2G097275_T01. [score:6]
For instance, the miR156 family had different targets, including SQUAMOSA promoter -binding protein-like and histidine-containing phosphotransfer factor 5. The genes encoding ARFs were identified as the targets of miR160 and miR167. [score:5]
Most of the targets were found to be transcription factors (TFs), such as auxin response factors (miR160 and miR167), growth -regulating factor (miR396), N-acetylcysteine domain containing protein (miR164), SQUAMOSA promoter -binding protein-like (miR156) and nuclear TF Y (miR169). [score:4]
miR166 consisted of 14 members, miR156 and miR167 had 12 and 10 members, respectively, and miR162, miR529, miR827 and miR1432 had only one member. [score:1]
In the identified miRNA families (Fig 2), miR166 consisted of 14 members, miR156 and miR167 had 12 and 10 members, respectively, and miR162, miR529, miR827 and miR1432 had only one member. [score:1]
0164026.g002 Fig 2 miR166 consisted of 14 members, miR156 and miR167 had 12 and 10 members, respectively, and miR162, miR529, miR827 and miR1432 had only one member. [score:1]
[1 to 20 of 6 sentences]
16
[+] score: 16
Other miRNAs from this paper: zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR394a, zma-MIR394b, zma-MIR396b, zma-MIR396a, zma-MIR399a, zma-MIR399c, zma-MIR399b, zma-MIR399d, zma-MIR399e, zma-MIR399f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR319a, zma-MIR319c, zma-MIR319b, zma-MIR319d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR393a, zma-MIR408a, zma-MIR156k, zma-MIR160f, zma-MIR396c, zma-MIR396d, zma-MIR2118a, zma-MIR2118b, zma-MIR2118c, zma-MIR2118d, zma-MIR2118e, zma-MIR2118f, zma-MIR2118g, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR390a, zma-MIR393b, zma-MIR393c, zma-MIR396e, zma-MIR396f, zma-MIR396g, zma-MIR396h, zma-MIR397a, zma-MIR397b, zma-MIR398a, zma-MIR398b, zma-MIR399g, zma-MIR399h, zma-MIR399i, zma-MIR399j, zma-MIR408b, zma-MIR528a, zma-MIR528b, zma-MIR827, zma-MIR390b, zma-MIR444a, zma-MIR444b
Expression levels of miR156, miR166, miR167, miR171 and miR827 decreased with the development of the embryo, but increased with the development of the endosperm, indicating that their functions are potentially involved in the switch from embryo to endosperm development (Figure 3b). [score:6]
SPL TFs were antagonized by miR156 and miR157, which function in the regulation of vegetative and reproductive development and fruit ripening in tomato, respectively [21, 22, 23]. [score:3]
Six miRNA families zma-miR166, zma-miR156, zma-miR171, zma-miR167, zma-miR169 and zma-miR399 were predominantly expressed in maize kernel (Figure 2c, Table S2). [score:3]
SQUAMOSA-promoter binding protein-like (SPL) TFs are negatively regulated by miR156, which function in the regulation of grain size, grain quality and grain yield in rice [12, 13, 14]. [score:3]
Among them, miR166 was the most abundant family (17,602) followed by miR171 (11,988), miR827 (7686), miR167, miR396, miR528, miR156, miR408, miR160, miR390, miR159, miR444, miR319, miR398, miR168, miR394, miR164, miR393 and miR169 (Figure 3a). [score:1]
[1 to 20 of 5 sentences]
17
[+] score: 14
In the present study, zma-miR156 was over dominantly repressed, which might cause over -expression of SPL transcription factors and result in enhancement of seed germination by influencing the expression of other transcription factors. [score:5]
Expression profiles were established based on stem-loop real-time RT-PCR analysis of six selected miRNAs, comprising miR156, miR164, miR167, miR168, miR169 and miR396, as well as real-time RT-PCR analysis of their target mRNAs. [score:5]
SQUAMOSA promoter -binding-like (SPL) family transcription factors are the target of the maize miRNA zma-miR156, which specially binds to the cis-element GTAC [45]. [score:3]
In the maize hybrid Yuyu22, miR167 was predominantly activated, whereas miR156, miR160, miR164 and miR166 were either dominantly or predominantly repressed. [score:1]
[1 to 20 of 4 sentences]
18
[+] score: 13
Other miRNAs from this paper: ath-MIR156a, ath-MIR156b, ath-MIR156c, ath-MIR156d, ath-MIR156e, ath-MIR156f, ath-MIR159a, ath-MIR160a, ath-MIR160b, ath-MIR160c, ath-MIR162a, ath-MIR162b, ath-MIR164a, ath-MIR164b, ath-MIR166a, ath-MIR166b, ath-MIR166c, ath-MIR166d, ath-MIR166e, ath-MIR166f, ath-MIR166g, ath-MIR167a, ath-MIR167b, ath-MIR169a, ath-MIR171a, ath-MIR172a, ath-MIR172b, ath-MIR159b, osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR162a, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, osa-MIR171a, ath-MIR167d, ath-MIR169b, ath-MIR169c, ath-MIR169d, ath-MIR169e, ath-MIR169f, ath-MIR169g, ath-MIR169h, ath-MIR169i, ath-MIR169j, ath-MIR169k, ath-MIR169l, ath-MIR169m, ath-MIR169n, ath-MIR171b, ath-MIR171c, ath-MIR172c, ath-MIR172d, ath-MIR393a, ath-MIR393b, ath-MIR394a, ath-MIR394b, ath-MIR395a, ath-MIR395b, ath-MIR395c, ath-MIR395d, ath-MIR395e, ath-MIR395f, osa-MIR393a, osa-MIR394, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, ath-MIR156g, ath-MIR156h, ath-MIR159c, ath-MIR164c, ath-MIR167c, ath-MIR172e, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR160e, osa-MIR160f, osa-MIR162b, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR171b, osa-MIR171c, osa-MIR171d, osa-MIR171e, osa-MIR171f, osa-MIR171g, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR171h, osa-MIR393b, osa-MIR172d, osa-MIR171i, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR164f, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR162, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR394a, zma-MIR394b, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR393a, zma-MIR156k, zma-MIR160f, osa-MIR528, osa-MIR529a, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, ath-MIR827, osa-MIR529b, osa-MIR1432, osa-MIR169r, osa-MIR827, osa-MIR2118a, osa-MIR2118b, osa-MIR2118c, osa-MIR2118d, osa-MIR2118e, osa-MIR2118f, osa-MIR2118g, osa-MIR2118h, osa-MIR2118i, osa-MIR2118j, osa-MIR2118k, osa-MIR2118l, osa-MIR2118m, osa-MIR2118n, osa-MIR2118o, osa-MIR2118p, osa-MIR2118q, osa-MIR2118r, osa-MIR2275a, osa-MIR2275b, zma-MIR2118a, zma-MIR2118b, zma-MIR2118c, zma-MIR2118d, zma-MIR2118e, zma-MIR2118f, zma-MIR2118g, zma-MIR2275a, zma-MIR2275b, zma-MIR2275c, zma-MIR2275d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR482, zma-MIR528a, zma-MIR528b, zma-MIR529, zma-MIR827, zma-MIR1432, osa-MIR395x, osa-MIR395y, osa-MIR2275c, osa-MIR2275d, ath-MIR156i, ath-MIR156j
MiRNA156 has been shown to be involved in floral development and phase change by targeting members of squamosa promoter binding protein like (SPL) plant-specific transcription factors. [score:3]
Recent results indicated that overexpression of miR156 affects phase transition from vegetative growth to reproductive growth, including the quickly initiation of rosette leaves, a severe decrease in apical dominance, and a moderate delay in flowering [58]. [score:3]
Not only the miRNA166 and miRNA156 families were abundant during this stage of seed germination, but also they had more family members than other miRNA families, suggesting the importance of these two miRNA families at this very early stage of seed germination. [score:1]
The largest miRNA family size identified was miR166 that consisted of 14 members and miR156, miR169 and miR167 possessed 12, 12 and 10 members, respectively; whereas other miRNA families such as miR162, miR529, miR827 and miR1432 had only one member detected in this period. [score:1]
For example, miR156/157, miR159/319, miR166, miR169, and miR394 have been found in 51, 45, 41, 40 and 40 plant species, respectively [36- 38]. [score:1]
In comparison to other plant species, tae-miR169b in wheat and osa-miR169 in rice are the most frequently sequenced miRNAs while miR156 in rice and wheat exhibits low abundance [32]. [score:1]
For example, the abundance of miR156 family varied from 261 reads (zma-miR156j) to 409,637 reads (zma-miR156d) in the deep sequencing. [score:1]
In our datasets, miRNA166 showed the highest abundance followed by miRNA156 and miRNA528, respectively, during the very early stage of seed germination. [score:1]
The abundance of zma-miR172 was extremely low compared to that of zma-miR156 in our dataset, which was consistent with previous finding that these two miRNAs are conversely regulated. [score:1]
[1 to 20 of 9 sentences]
19
[+] score: 13
MiR156 targeted SBP transcription factor [52, 53], which affected shoot maturation in Arabidopsis, miR159c/d and miR164 were predicted to target MYB domain transcription factors [24, 54], and miR160 targeted ARF transcription factors [55, 56]. [score:7]
In Arabidopsis, miR156 targets SPL10 (Squamosa Promoter-Binding Protein-Like 10) and SPL11, and over accumulation of these targets leads to abnormal cell divisions [18]. [score:5]
Other miRNAs in high abundance include zma-miR164, zma-miR156 and zma-miR827, which were more than 1,000 reads in both two tissues. [score:1]
[1 to 20 of 3 sentences]
20
[+] score: 12
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, osa-MIR396a, osa-MIR396b, osa-MIR396c, osa-MIR397a, osa-MIR397b, osa-MIR398a, osa-MIR398b, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR160e, osa-MIR160f, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR171b, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR172d, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR164f, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, osa-MIR396e, zma-MIR396b, zma-MIR396a, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR156k, zma-MIR160f, tae-MIR159a, tae-MIR159b, tae-MIR160, tae-MIR164, tae-MIR167a, tae-MIR1127a, osa-MIR169r, osa-MIR396f, zma-MIR396c, zma-MIR396d, osa-MIR2275a, osa-MIR2275b, zma-MIR2275a, zma-MIR2275b, zma-MIR2275c, zma-MIR2275d, osa-MIR396g, osa-MIR396h, osa-MIR396d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR396e, zma-MIR396f, zma-MIR396g, zma-MIR396h, zma-MIR397a, zma-MIR397b, zma-MIR398a, zma-MIR398b, hvu-MIR156a, tae-MIR156, hvu-MIR159b, hvu-MIR159a, hvu-MIR166a, tae-MIR167b, hvu-MIR168, hvu-MIR169, tae-MIR169, hvu-MIR397a, tae-MIR398, tae-MIR171b, hvu-MIR166b, hvu-MIR166c, osa-MIR2275c, osa-MIR2275d, tae-MIR1122b, tae-MIR9653a, tae-MIR9654a, tae-MIR9656, tae-MIR9657a, tae-MIR9659, tae-MIR9660, tae-MIR1127b, tae-MIR9661, tae-MIR396, tae-MIR9665, tae-MIR2275, tae-MIR9667, tae-MIR167c, tae-MIR1120b, tae-MIR397, tae-MIR1130b, tae-MIR5384, tae-MIR9675, tae-MIR1120c, tae-MIR9679, tae-MIR9657b, hvu-MIR397b, hvu-MIR156b, tae-MIR9653b
miR156 targets squamosa promoter -binding protein-like 10 (SPL10) and SPL11, and the regulation of these targets prevents premature gene expression during early embryogenesis [19]. [score:8]
Of the 15 known miRNA families, 8 (miR396, miR168, miR156, miR172, miR159, miR398, miR1318 and miR167) showed different levels of preferential expression in wheat flag leaves, with the logarithm of the fold changes ranged from 0.5 to 5.2 as well as more than those in the developing seeds (Figure  3a, Table  2). [score:3]
The highest read abundance (approximately 238,000 RPM) was detected in the miR168 family and was 3.8 to 78 times more abundant than the other miRNA families, including miR156, miR166, miR167 and miR172, whose abundance ranged from about 2,900 RPM to 62,000 RPM (Table  2). [score:1]
[1 to 20 of 3 sentences]
21
[+] score: 12
Other miRNAs from this paper: zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR394a, zma-MIR394b, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR396b, zma-MIR396a, zma-MIR399a, zma-MIR399c, zma-MIR399b, zma-MIR399d, zma-MIR399e, zma-MIR399f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR319a, zma-MIR319c, zma-MIR319b, zma-MIR319d, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR171k, zma-MIR171h, zma-MIR408a, zma-MIR156k, zma-MIR160f, zma-MIR396c, zma-MIR396d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR396e, zma-MIR396f, zma-MIR396g, zma-MIR396h, zma-MIR399g, zma-MIR399h, zma-MIR399i, zma-MIR399j, zma-MIR408b, zma-MIR529
Among them, zma-miR167, zma-miR171,and zma-miR172 were down-regulated in both inbred lines, while zma-miR156, zma-miR395, zma-miR399, zma-miR408, and zma-miR529 were up-regulated in both inbred lines between 20 and 30DAP. [score:7]
Among those identified in the leaves of inbred line ELS-1, differentially expressed miRNAs whose |log [2]-fold change| was >1.5 in the leaves of inbred line Yu87-1 were removed, and 16 candidate miRNAs were finally identified in the leaves of inbred line ELS-1. These 16 differentially expressed miRNAs, belonging to nine miRNA families, zma-miR156, zma-miR159, zma-miR167, zma-miR171, zma-miR172, zma-miR395, zma-miR399, zma-miR408, and zma-miR529, were selected as candidate SA-miRNAs (Fig.   3). [score:5]
[1 to 20 of 2 sentences]
22
[+] score: 12
The results indicated that miR156, miR171, miR396 and miR444, which respectively targeted to SPL, SCL, QQT and EDA genes, might differentially expressed in the seed development in two maize inbred lines, especially involved in flowering regulation and embryo development. [score:8]
The results indicated that some miRNAs (e. g. miR156, miR171, miR396 and miR444) differentially expressed in the seed development between PH6WC and PH4CV maize inbred lines under different genetic backgrounds. [score:4]
[1 to 20 of 2 sentences]
23
[+] score: 9
For example, miR162 was not found in our research; miR156, miR164, miR167, and miR396 were not down-regulated in the roots, but in the leaves, miR164 showed a down-regulation. [score:7]
The abundance of extremely conserved miRNAs has been determined, such as miR156, miR159, miR164, miR166, miR167, miR168, and miR398, which was very similar to previous reports (Zhao et al., 2013). [score:1]
For instance, the abundance of zma-miR156 and zma-miR167 was 40560.15 reads per million (RPM = specific miRNA reads [∗]10 [6]/total reads) and 3714.984 RPM, respectively. [score:1]
[1 to 20 of 3 sentences]
24
[+] score: 8
The challenge here, as with the case of transgenic switchgrass in which MYB4 (Shen et al., 2012) and miRNA156 (Fu et al., 2012) gene expression was changed, is to empirically determine the optimal TF transgene expression level that decreases lignin and possibly provides favorable plant architecture changes, without reducing shoot growth. [score:5]
Overexpression of miR156 in switchgrass (Panicum virgatum L. ) results in various morphological alterations and leads to improved biomass production. [score:3]
[1 to 20 of 2 sentences]
25
[+] score: 8
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR169a, osa-MIR393a, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR168a, osa-MIR168b, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR393b, osa-MIR166m, osa-MIR166j, osa-MIR164f, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, osa-MIR444a, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR166l, zma-MIR166m, zma-MIR393a, zma-MIR156k, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR820a, osa-MIR820b, osa-MIR820c, osa-MIR1425, osa-MIR1428a, osa-MIR169r, osa-MIR444b, osa-MIR444c, osa-MIR444d, osa-MIR444e, osa-MIR444f, osa-MIR1428b, osa-MIR1428c, osa-MIR1428d, osa-MIR1428e, osa-MIR1874, osa-MIR2055, osa-MIR827, osa-MIR1428f, osa-MIR1428g, zma-MIR396d, osa-MIR396d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR827, osa-MIR395x, osa-MIR395y, zma-MIR444a, zma-MIR444b
This tandem array is functionally important as in maize over -expression of a tandem miR156 gene produces the Corngrass1 heterochronic mutant [39]. [score:3]
The functional significance for expressing tandem array of similar miRNAs is not clear, but it has been shown for the rice/maize miR156 and the Medicago miR166. [score:3]
These correspond to miR156 and miR395 in rice and maize [37- 39] and miR166 in Medicago truncatula [40]. [score:1]
In the case of the rice osa-miR156 is encoded by 12 loci, but only one of them encodes a tandem MIR156b-156c precursor [37]. [score:1]
[1 to 20 of 4 sentences]
26
[+] score: 8
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR171a, osa-MIR393a, osa-MIR397a, osa-MIR397b, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR319b, osa-MIR166k, osa-MIR166l, osa-MIR168a, osa-MIR168b, osa-MIR169f, osa-MIR171b, osa-MIR171c, osa-MIR171d, osa-MIR171e, osa-MIR171f, osa-MIR171g, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR171h, osa-MIR393b, osa-MIR172d, osa-MIR171i, osa-MIR166m, osa-MIR166j, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR319b, zma-MIR166k, zma-MIR166j, zma-MIR168a, zma-MIR168b, zma-MIR169f, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR393a, zma-MIR156k, osa-MIR529a, tae-MIR159a, tae-MIR159b, tae-MIR171a, tae-MIR1120a, osa-MIR1430, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR166n, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR393b, zma-MIR393c, zma-MIR397a, zma-MIR397b, hvu-MIR156a, tae-MIR156, hvu-MIR159b, hvu-MIR159a, hvu-MIR166a, hvu-MIR168, hvu-MIR171, hvu-MIR397a, tae-MIR171b, hvu-MIR1120, hvu-MIR166b, osa-MIR3981, hvu-MIR166c, tae-MIR1120b, tae-MIR397, tae-MIR1120c, hvu-MIR397b, hvu-MIR156b
Both 20 and 21 nt long miR156 were expressed at the highest level in 68-day-old plants. [score:3]
The 20 nt long mature miR156 was previously identified in barley using deep sequencing [48]. [score:1]
Hybridization also revealed the presence of two mature miR156, 20 and 21 nt long (Figure 6H). [score:1]
A 21 nt long mature miR156 with an additional adenosine residue at the 3 [′] end is annotated in the databases of many eukaryotic species [50, 51]. [score:1]
Based on nucleotide sequence and structural similarities, we classify barley MIR156 as an orthologue of rice MIR156g (Figure 6B). [score:1]
Both the 20 and 21 nt miR156 species were equally represented in 6-week- and 68-day-old plants; however, in 1- and 2-week-old plants, primarily the 20 nt long miR156 was detectable. [score:1]
[1 to 20 of 6 sentences]
27
[+] score: 8
Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156 -targeted SPL transcription factor. [score:4]
MiR156 and MiR159 for example target SPL and MYB transcription factors, and their overexpression can cause late flowering and male sterility (Achard et al., 2004; Millar and Gubler, 2005; Schwab et al., 2005). [score:4]
[1 to 20 of 2 sentences]
28
[+] score: 6
Unexpectedly, miR156, which represses the juvenile to adult phase transition [41], is upregulated in lbl1. [score:4]
It is conceivable that rather than contributing to the phenotype, the changes in miR156 levels are a consequence of the lbl1 phenotype. [score:1]
Arabidopsis ta-siRNA biogenesis mutants exhibit an accelerated transition from the juvenile to the adult phase [2], [9], whereas increased levels of miR156 in lbl1 might imply a delayed vegetative phase change. [score:1]
[1 to 20 of 3 sentences]
29
[+] score: 6
For example, the target of miR156/157 is Colorless Non-Ripening (CNR) (Karlova et al., 2013), an epigenetic mutation of which could inhibit tomato fruit ripening (Manning et al., 2006). [score:6]
[1 to 20 of 1 sentences]
30
[+] score: 5
The miR156/SPL module is highly conserved among the phylogenetically distinct plant species, and plays important roles in regulating plant fitness, biomass, and yield [47]. [score:2]
Wang H. Wang H. The miR156/SPL Module, a Regulatory Hub and Versatile Toolbox, Gears up Crops for Enhanced Agronomic TraitsMol. [score:2]
The nine families comprised miR156, miR166, miR167, miR171, miR396, miR398, miR408, miR444, and miR827. [score:1]
[1 to 20 of 3 sentences]
31
[+] score: 5
Other miRNAs from this paper: zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR399a, zma-MIR399c, zma-MIR399b, zma-MIR399d, zma-MIR399e, zma-MIR399f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR393a, zma-MIR156k, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR390a, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR398a, zma-MIR398b, zma-MIR399g, zma-MIR399h, zma-MIR399i, zma-MIR399j, zma-MIR528a, zma-MIR528b, zma-MIR529, zma-MIR827, zma-MIR390b
Expression of miR156 increased linearly and was the highest at 18–23 DAP, which was consistent with the results seen in Zhengdan 958 [72], as well as in rice [96], wheat [97] and barley [98]. [score:3]
Conserved miRNAs that accumulated more in the late developmental stage of maize seed include miR156, miR167, miR398, and miR528 families. [score:2]
[1 to 20 of 2 sentences]
32
[+] score: 5
Lastly, a number of genes involved in phase change and the regulation of the length of the vegetative growth phase were also identified (SPL9, SPL11, miR156), suggesting that phase change and flowering time are intimately connected. [score:2]
96: 31– 36 Wang J. W. Czech B. Weigel D., 2009  miR156-regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana. [score:2]
Phase change genes such as SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9 (SPL9) and SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 11 (SPL11), and the microRNA miR156 were also identified as candidates for differences in flowering time. [score:1]
[1 to 20 of 3 sentences]
33
[+] score: 4
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR171a, osa-MIR393a, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR396a, osa-MIR396b, osa-MIR156k, osa-MIR156l, osa-MIR166k, osa-MIR166l, osa-MIR168a, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR393b, osa-MIR408, osa-MIR172d, osa-MIR166m, osa-MIR166j, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR396b, zma-MIR396a, zma-MIR156j, zma-MIR166k, zma-MIR166j, zma-MIR168a, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR393a, zma-MIR408a, zma-MIR156k, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR1432, zma-MIR156l, zma-MIR166n, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR408b, zma-MIR482, zma-MIR1432, osa-MIR395x, osa-MIR395y
The expression of teosinte glume architecture1 (tga1), which plays an important role in maize domestication, is regulated by miR156 [28]. [score:4]
[1 to 20 of 1 sentences]
34
[+] score: 4
Negative regulation of anthocyanin biosynthesis in Arabidopsis by a miR156 -targeted SPL transcription factor. [score:4]
[1 to 20 of 1 sentences]
35
[+] score: 4
Other miRNAs from this paper: zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR171d, zma-MIR171f, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR396b, zma-MIR396a, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR393a, zma-MIR156k, zma-MIR160f, zma-MIR396c, zma-MIR396d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR390a, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR396e, zma-MIR396f, zma-MIR396g, zma-MIR396h, zma-MIR528a, zma-MIR528b, zma-MIR827
miR156 is involved in diverse processes in various mo del species, including anther development, juvenile-to-adult phase transition, gibberellin signal transduction, and flower and leaf development [18]– [21]. [score:3]
In maize, however, the accumulation of zma-miR156 in pollen and silk samples suggests its possible role in reproductive processes. [score:1]
[1 to 20 of 2 sentences]
36
[+] score: 3
In Arabidopsis, the expression of miR156, miR167, miR168, and miR396 increased 2 h to 24 h after exposure to high-salinity treatment. [score:3]
[1 to 20 of 1 sentences]
37
[+] score: 3
In particular, miR156 miR162 exhibited pronounced changes in expression under severe drought stress, suggesting that they may contribute to the acclimatization process [15]. [score:3]
[1 to 20 of 1 sentences]
38
[+] score: 3
The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis. [score:3]
[1 to 20 of 1 sentences]
39
[+] score: 3
Plant miRNAs are described that function in the ARGONAUTE1-directed regulation of leaf initiation and polarity, including miR166, and miR156 (reviewed in [73], [74]). [score:3]
[1 to 20 of 1 sentences]
40
[+] score: 2
Other miRNAs from this paper: osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR156k, osa-MIR156l, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR167j, osa-MIR166m, osa-MIR166j, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, gma-MIR156d, gma-MIR156e, gma-MIR156c, gma-MIR166a, gma-MIR166b, gma-MIR167a, gma-MIR167b, gma-MIR168a, gma-MIR156a, gma-MIR156b, zma-MIR156j, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR166l, zma-MIR166m, zma-MIR156k, osa-MIR535, gma-MIR167c, gma-MIR1507a, gma-MIR167d, gma-MIR1507b, gma-MIR167e, gma-MIR167f, zma-MIR156l, zma-MIR166n, zma-MIR167j, gma-MIR167g, gma-MIR156f, gma-MIR156g, gma-MIR156h, gma-MIR156i, gma-MIR166c, gma-MIR166d, gma-MIR166e, gma-MIR166f, gma-MIR166g, gma-MIR166h, gma-MIR168b, gma-MIR1507c, gma-MIR167h, gma-MIR167i, gma-MIR3522, gma-MIR156j, gma-MIR156k, gma-MIR156l, gma-MIR156m, gma-MIR156n, gma-MIR156o, gma-MIR166i, gma-MIR166j, gma-MIR167j, gma-MIR156p, gma-MIR156q, gma-MIR156r, gma-MIR156s, gma-MIR166k, gma-MIR156t, gma-MIR166l, gma-MIR166m, gma-MIR156u, gma-MIR156v, gma-MIR156w, gma-MIR156x, gma-MIR156y, gma-MIR156z, gma-MIR156aa, gma-MIR156ab, gma-MIR166n, gma-MIR166o, gma-MIR166p, gma-MIR166q, gma-MIR166r, gma-MIR166s, gma-MIR166t, gma-MIR166u, gma-MIR167k, gma-MIR167l
The second and third most abundant miRNAs in the pig abdominal fat dataset are miR156 and miR168, with 1584 and 1085 reads, respectively (Additional file 2). [score:1]
The plant miRNAs used in the search include miR156, miR166, miR167, miR168, miR535 and miR3522. [score:1]
[1 to 20 of 2 sentences]
41
[+] score: 2
Very recently, SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 15 (AtSPL) was demonstrated to be regulated by miR156 and to promote flowering under non-inductive conditions (Hyun et al., 2016). [score:2]
[1 to 20 of 1 sentences]
42
[+] score: 2
Overexpression of microRNA156 in Brassica napus enhanced carotenoid content in seeds (Wei et al., 2010). [score:2]
[1 to 20 of 1 sentences]
43
[+] score: 2
Jung JH Seo PJ Kang SK Park CM miR172 signals are incorporated into the miR156 signaling pathway at the SPL3/4/5 genes in Arabidopsis developmental transitionsPlant Mol. [score:2]
[1 to 20 of 1 sentences]
44
[+] score: 2
It was found that SPLs participate as a last resort flowering mechanism activated by the age of the plant and is naturally repressed in young plants by miRNA156 [25]. [score:1]
In this manner, when miRNA156 was more expressed in natural mutants or genetically engineered plants, the juvenile phase was extended, and, interestingly, starch and cell wall saccharification were increased [62]; this constitutes one of the few examples where these two highly desired characteristics in plant biomass for biofuels are simultaneously improved. [score:1]
[1 to 20 of 2 sentences]
45
[+] score: 1
The dominant Corngrass1 (Cg1) mutant encodes two tandem zma- miR156 genes and leads to a small ear lacking an ordered kernel row and unbranched tassel (Chuck et al. 2007). [score:1]
[1 to 20 of 1 sentences]
46
[+] score: 1
Interestingly, there have been previous reports of enhanced susceptibility to insect herbivory when the juvenile phase and purple TBO staining of leaf epidermal cells is prolonged by the maize Corngrass1 mutation [41]– [44] that overproduces microRNA156 (45). [score:1]
[1 to 20 of 1 sentences]
47
[+] score: 1
Accordingly, genes known to be involved in maize plastochron (corngrass1/mir156 mapped on chr. [score:1]
[1 to 20 of 1 sentences]
48
[+] score: 1
Vegetative phase change is mediated by a leaf-derived signal that represses the transcription of miR156. [score:1]
[1 to 20 of 1 sentences]
49
[+] score: 1
The most abundant family was miR159, followed by miR319, miR168 and miR156 (Table 2). [score:1]
[1 to 20 of 1 sentences]
50
[+] score: 1
1. Hwan Lee J. Joon Kim J. Ahn J. H. Role of SEPALLATA3 (SEP3) as a downstream gene of miR156-SPL3- FT circuitry in ambient temperature-responsive flowering Plant Signal. [score:1]
[1 to 20 of 1 sentences]
51
[+] score: 1
A candidate gene for the Br5a QTL region is corngrass1, which in maize encodes two tandem miR156 genes, and where the mutant produces tillers in the axil of each leaf [92], while for H5a a candidate gene is SHOOTLESS2, a gene involved in initiation and maintenance of the shoot apical meristem [93]. [score:1]
[1 to 20 of 1 sentences]