sort by

3 publications mentioning aga-let-7

Open access articles that are associated with the species Anopheles gambiae and mention the gene name let-7. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 9
When D. melanogaster data are available for comparison as in the cases of let-7 and miR-9a, similar expression profiles were found between An. [score:3]
Let-7 expression begins in late larvae in mosquitoes and continues into adult, again similar to what was observed in D. melanogaster. [score:2]
After initial size confirmation using let-7 RNA, 19 nt and 23 nt single-stranded DNA oligos were used as size markers in subsequent experiments. [score:1]
Northern analysis using total RNA from 17-day old females with antisense Locked Nucleic Acid (LNA) probes against 4 selected miRNAs (miR-9a, -14, -210, and let-7) all showed bands of the correct size, confirming cloning results (Figure 1, the last lane). [score:1]
They are let-7, miR-281, miR-34, miR-12, and miR-306. [score:1]
Shown here are eight northern blots performed using Dig-labeled miRCURY LNA probes designed for hybridization to either miR-14, let-7, miR-9a, miR-210, or to one of the four novel miRNAs (miR-x1–x4). [score:1]
[1 to 20 of 6 sentences]
2
[+] score: 5
Strikingly, in A. gambiae, 80% of nontemplate-directed urydilated reads derived from miR-8. The number of described 3’ additions in this study might be significantly underestimated since certain adenine and uracil additions map to adjacent genomic sequence of the pre-miRNA (e. g. miR-281, let-7 or bantam) and therefore, it was not possible to discern unambiguously between template- and nontemplate-directed additions. [score:3]
In addition, we observed that miRNA loci, such as mir-277, let-7, mir-1174 and mir-279 produced from one fourth to one third of sequence reads tailed by template-directed adenine addition. [score:2]
[1 to 20 of 2 sentences]
3
[+] score: 4
recognition sites Mean MFE (Kcal/Mol) Recognition site start position on lincRNA_1317 miR-278-5p 5 -22.40 307, 749, 1112, 1260, 1491 miR-252-3p 4 -21.15 162, 629, 1560, 3946 miR-11-5p 3 -21.67 2248, 2286, 3328 miR-1890 3 -21.17 1489, 2712, 3602 miR-263a-3p 3 -21.47 2208, 2545, 3336 miR-33 3 -24.90 1545, 1810, 2669 miR-34-5p 3 -25.03 1020, 1232, 1379 miR-9b 3 -24.03 2034, 2891, 3603 let-7 2 -22.20 2747, 2817 miR-1 2 -22.80 1165, 2528 miR-1175-3p 2 -20.65 1489, 3274 miR-12-5p 2 -25.60 632, 1575 miR-1889-3p 2 -20.80 1268, 3988 miR-1891 2 -21.10 162, 2349 miR-282-5p 2 -25.70 1232, 1297 miR-2944b-3p 2 -22.05 1255, 3797 miR-2945-5p 2 -23.00 770, 1209 miR-31 2 -25.70 819, 871 miR-375 2 -22.25 66, 3293 miR-92b-5p 2 -23.05 1042, 3788 miR-9a 2 -20.65 170, 3500 We also used LncTar algorithm to predict any direct interaction between lincRNA_1317 and DENV-2 genome. [score:2]
recognition sites Mean MFE (Kcal/Mol) Recognition site start position on lincRNA_1317 miR-278-5p 5 -22.40 307, 749, 1112, 1260, 1491 miR-252-3p 4 -21.15 162, 629, 1560, 3946 miR-11-5p 3 -21.67 2248, 2286, 3328 miR-1890 3 -21.17 1489, 2712, 3602 miR-263a-3p 3 -21.47 2208, 2545, 3336 miR-33 3 -24.90 1545, 1810, 2669 miR-34-5p 3 -25.03 1020, 1232, 1379 miR-9b 3 -24.03 2034, 2891, 3603 let-7 2 -22.20 2747, 2817 miR-1 2 -22.80 1165, 2528 miR-1175-3p 2 -20.65 1489, 3274 miR-12-5p 2 -25.60 632, 1575 miR-1889-3p 2 -20.80 1268, 3988 miR-1891 2 -21.10 162, 2349 miR-282-5p 2 -25.70 1232, 1297 miR-2944b-3p 2 -22.05 1255, 3797 miR-2945-5p 2 -23.00 770, 1209 miR-31 2 -25.70 819, 871 miR-375 2 -22.25 66, 3293 miR-92b-5p 2 -23.05 1042, 3788 miR-9a 2 -20.65 170, 3500We also used LncTar algorithm to predict any direct interaction between lincRNA_1317 and DENV-2 genome. [score:2]
[1 to 20 of 2 sentences]