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14 publications mentioning rno-mir-449a

Open access articles that are associated with the species Rattus norvegicus and mention the gene name mir-449a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 108
Other miRNAs from this paper: rno-mir-449c
CGRP mRNA is a direct target for miR-449a and the stimulation of EA on CGRP level is achieved by downregulation of miR-449a expression. [score:9]
It was found that miR-449a agomir upregulated the miR-449a level in these rats, which inhibited the high expression of Nestin, NeuN and CGRP. [score:8]
Effect of miR-449a agomir on EA-stimulated Nestin, NeuN and CGRP expression and EA-reduced level of cleaved caspase 3, TNF-α and IL-1β and the ratio of Bax to Bcl-2. miR-449a regulates CGRP expression by directly binding to its 3'UTR. [score:7]
Our study showed EA could suppress the SCI -induced elevation of cleaved caspase 3, Bax/Bcl-2 ratio, TNF-α and IL-1β, which indicates that EA may inhibit apoptosis and inflammation through attenuating the mature of caspase-3 and expression of TNF-α and IL-1β and the ratio of Bax/Bcl-2. MiR-449a is a recently identified member of the conserved miR-449 family. [score:7]
Electro-acupuncture downregulated miR-449a and promote the expression of NSC biomarker Nestin and neuron biomarker NeuN. [score:6]
It was found that miR-449a mimic specifically bound to the 3'UTR of CGRP mRNA, revealing that miR-449a directly regulated the CGRP gene expression on the post-transcription level. [score:5]
These results suggest that high level of miR-449a could suppress the effect of EA on the expression of Nestin, NeuN, CGRP, cleaved caspase 3, Bax, Bcl-2, TNF-α and IL-1β in spinal cord of rats. [score:5]
High level of miR-449a promoted the mature of caspase-3 and expression of TNF-α and IL-1β and the ratio of Bax/Bcl-2 which were suppressed by EA in ventral horn of injured spinal cord of rats. [score:5]
Studies have shown that miR-449 a/b level in liver cancer, bladder cancer, prostate cancer and gastric cancer is markedly lower compared with normal tissues, and exogenous miR-449 a/b expression can inhibit cell proliferation, and induce cell cycle arrest, senescence and apoptosis (Bou Kheir et al., 2011[8]; Buurman et al., 2012[9]; Chen et al., 2012[13]; Noonan et al., 2009[27]). [score:4]
The regulation of miR-449a on the CGRP expression was further determined by. [score:4]
Further, the regulation of miR-449a on EA-stimulated Nestin, NeuN and CGRP expression was assessed by comparing rats receiving EA treatment and EA+miR-449a agomir treatment. [score:4]
The miR-449a expression in SCI group was significantly increased compared with that in sham-operated group (P < 0.05), whereas miR-449a expression was significantly reduced after EA treatment (P < 0.01, Figure 1A (Fig. 1)). [score:4]
Studies have found that miR-449a level is decreased in the trigeminal ganglia of migraine rats and the protein might be involved in the regulation of CGRP expression (Zhang et al., 2015[39]). [score:4]
Effects of EA on Nestin, NeuN, CGRP and miR-449a expression. [score:3]
Luciferase reports assay showed a direct target site of miR-449a in 3' UTR of CGRP mRNA. [score:3]
The result suggested that miR-449a mediated the stimulative effect of EA on Nestin, NeuN and CGRP expression. [score:3]
A. Predicted binding site between miR-449a and CGRP 3' UTR and the contrived mutant site in CGRP 3' UTR; B. miR-449a mimic targeted at CGRP 3' UTR and degraded luciferase mRNA. [score:3]
miR-449 may silence the target gene by binding to its 3'-UTR region (Tsujiura et al., 2010[35]). [score:3]
When compared with negative control group, miR-449a mimics significantly reduced the RLA in HEK 293T cells transfected with pMIR- CGRP-UTR (RLA = 0.47, P < 0.01), but did not affect the RLA in pMIR-CGRP-UTR-mut group (Figure 4B (Fig. 4)), indicating that miR-449a could recognize and bind to the 3'-UTR of CGRP mRNA and regulate the CGRP expression. [score:3]
As shown in Figure 3A (Fig. 3), miR-449a expression in miR group was significantly higher than that in EA group (P < 0.05). [score:3]
Nevertheless, although miR-449a is well studied in a variety of cancers, its regulatory role in post-SCI neurological functional recovery has seldomly been studied. [score:2]
Primers used in PCR amplification and miR-449a agomir (pre-miR-449a: 5'-CUGUGUGCGAUGGGUUGGCAGUGUAUUGUUAGCUGGUUGAGUAUGUAAAAGGCACCAGCUAACAUGCAACUGCUCUCCUAUUGCACAUACA-3') and the non -targeting sequence (negative control) were synthesized by GenePharma Biotech. [score:2]
MiR-449 mediated the promotive effect of EA on the expression of NSC biomarker Nestin and neuron biomarker NeuN. [score:2]
Mutagenesis was performed at the miR-449a binding site using the Quick-change site-directed mutagenesis kit. [score:2]
For instance, miR-449a antagonist may be used in combination with EA to improve the treatment outcome of SCI. [score:1]
A. Differences of miR-449a among groups; B. MRNA levels of Nestin, NeuN and CGRP in each group; C. Protein levels of Nestin, NeuN and CGRP detected by Western blotting in each group; D. Densitometric analysis results of Western blots; E. Representative immunohistochemical stain of Nestin, NeuN and CGRP in ventral horn of spinal cord; F-H. Quantification of immunohistochemical stain of Nestin (F), NeuN (G) and CGRP (H). [score:1]
org) to predict the 3'UTR sequence for miRNA-449a. [score:1]
miR-449a mimic was synthesized by GenePharma Biotech. [score:1]
CGRP was predicted to have a binding site in the 3'UTR sequence for miRNA-449a (Figure 4A (Fig. 4)). [score:1]
In the current study, SCI rats were treated by EA combined with intraperitoneal injections of miR-449a agomir. [score:1]
Furthermore, the regulatory mechanism of miR-449a on CGRP gene expression was also investigated by luciferase reporter assay. [score:1]
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2
[+] score: 32
Analysis at P21 revealed 74 mature miRNAs with differential expression between LPD -induced IUGR rat lungs and control lungs (Fig 1): 10 showed more than twofold differential expression: miR-184, miR-127-3p, miR-378a-5p and miR541-5p were downregulated, and miR-30e-5p, miR-23b-5p, miR-451-5p, miR-1839-5p, miR-449a-5p, and miR-19b-3p were upregulated in LPD -induced IUGR versus control lungs. [score:11]
Furthermore, five deregulated miRNAs (miR-128-3p and miR-34c-5p, both downregulated at P10; miR-19b-3p, miR-449a-5p and miR-30e-5p, these three being upregulated at P21) have E2F3 in common as a target gene. [score:10]
At P21, the differential expression of 5 of 10 of the miRNAs was statistically confirmed, with miR378a-5p, miR127-3p, and miR184 downregulated and miR30e-5p and miR449a-5p upregulated. [score:9]
Despite the differences in animal mo dels and in miRNA microarrays used and the fact that miRNA characterization is ongoing, Xing et al. and Zhang et al. also found upregulation of miR-449a-5p during the last stages of impaired alveolarization. [score:2]
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3
[+] score: 14
Impaired miR449a -induced downregulation of Crhr1 expression in low-birth-weight rats. [score:6]
miR-449a contributes to glucocorticoid -induced CRF-R1 downregulation in the pituitary during stress. [score:4]
Pituitary MicroR-449a (miR-449a) impairment induced a decrease in the level of CRH receptor 1 (CRHR1) level in low-birth-weight rats (Nemoto et al., 2015), and miR-449a was involved in the GC induced CRHR1 downregulation (Nemoto et al., 2013). [score:4]
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4
[+] score: 9
On the other hand, miR-192 and miR-194 were highly expressed in the kidney and small intestine, and miR-449a was highly expressed in the lung (Figures 3(d) and 3(e)). [score:5]
The expression of miR-200a, miR-200b, miR-200c, miR-192, miR-194, and miR-449a was validated with real-time RT-PCR in rat tissues in order to discriminate the kidney from other tissues with a tubular structure. [score:3]
In addition, we identified miR-449a as a lung-specific miRNA in rodents in the present study. [score:1]
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5
[+] score: 9
MiR-449a regulates autophagy to inhibit silica -induced pulmonary fibrosis through targeting Bcl2. [score:5]
Overexpression of MiR-449a significantly activated autophagy and reduced the extent and severity of lung fibrosis induced by silica through targeting Bcl2 (Han et al., 2016). [score:4]
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6
[+] score: 7
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-26b, hsa-mir-29a, hsa-mir-30a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-106a, mmu-let-7g, mmu-let-7i, mmu-mir-15b, mmu-mir-29b-1, mmu-mir-30a, mmu-mir-30b, mmu-mir-125a, mmu-mir-125b-2, mmu-mir-130a, mmu-mir-138-2, mmu-mir-181a-2, mmu-mir-182, hsa-mir-30c-2, hsa-mir-30d, mmu-mir-30e, hsa-mir-10a, hsa-mir-34a, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-182, hsa-mir-181a-1, mmu-mir-297a-1, mmu-mir-297a-2, mmu-mir-301a, mmu-mir-34c, mmu-mir-34b, mmu-let-7d, mmu-mir-106a, mmu-mir-106b, hsa-let-7g, hsa-let-7i, hsa-mir-15b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-130a, hsa-mir-138-2, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-138-1, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-30d, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-15a, mmu-mir-26b, mmu-mir-29a, mmu-mir-29c, mmu-mir-34a, rno-mir-301a, rno-let-7d, rno-mir-344a-1, mmu-mir-344-1, rno-mir-346, mmu-mir-346, rno-mir-352, hsa-mir-181b-2, mmu-mir-10a, mmu-mir-181a-1, mmu-mir-29b-2, mmu-mir-138-1, mmu-mir-181b-1, mmu-mir-181c, mmu-mir-125b-1, hsa-mir-106b, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-34b, hsa-mir-34c, hsa-mir-301a, hsa-mir-30e, hsa-mir-362, mmu-mir-362, hsa-mir-369, hsa-mir-374a, mmu-mir-181b-2, hsa-mir-346, rno-let-7a-1, rno-let-7a-2, rno-let-7b, rno-let-7c-1, rno-let-7c-2, rno-let-7e, rno-let-7f-1, rno-let-7f-2, rno-let-7i, rno-mir-10a, rno-mir-15b, rno-mir-26b, rno-mir-29b-2, rno-mir-29a, rno-mir-29b-1, rno-mir-29c-1, rno-mir-30c-1, rno-mir-30e, rno-mir-30b, rno-mir-30d, rno-mir-30a, rno-mir-30c-2, rno-mir-34b, rno-mir-34c, rno-mir-34a, rno-mir-106b, rno-mir-125a, rno-mir-125b-1, rno-mir-125b-2, rno-mir-130a, rno-mir-138-2, rno-mir-138-1, rno-mir-181c, rno-mir-181a-2, rno-mir-181b-1, rno-mir-181b-2, rno-mir-181a-1, hsa-mir-449a, mmu-mir-449a, mmu-mir-463, mmu-mir-466a, hsa-mir-483, hsa-mir-493, hsa-mir-181d, hsa-mir-499a, hsa-mir-504, mmu-mir-483, rno-mir-483, mmu-mir-369, rno-mir-493, rno-mir-369, rno-mir-374, hsa-mir-579, hsa-mir-582, hsa-mir-615, hsa-mir-652, hsa-mir-449b, rno-mir-499, hsa-mir-767, hsa-mir-449c, hsa-mir-762, mmu-mir-301b, mmu-mir-374b, mmu-mir-762, mmu-mir-344d-3, mmu-mir-344d-1, mmu-mir-673, mmu-mir-344d-2, mmu-mir-449c, mmu-mir-692-1, mmu-mir-692-2, mmu-mir-669b, mmu-mir-499, mmu-mir-652, mmu-mir-615, mmu-mir-804, mmu-mir-181d, mmu-mir-879, mmu-mir-297a-3, mmu-mir-297a-4, mmu-mir-344-2, mmu-mir-466b-1, mmu-mir-466b-2, mmu-mir-466b-3, mmu-mir-466c-1, mmu-mir-466e, mmu-mir-466f-1, mmu-mir-466f-2, mmu-mir-466f-3, mmu-mir-466g, mmu-mir-466h, mmu-mir-493, mmu-mir-504, mmu-mir-466d, mmu-mir-449b, hsa-mir-374b, hsa-mir-301b, rno-mir-466b-1, rno-mir-466b-2, rno-mir-466c, rno-mir-879, mmu-mir-582, rno-mir-181d, rno-mir-182, rno-mir-301b, rno-mir-463, rno-mir-673, rno-mir-652, mmu-mir-466l, mmu-mir-669k, mmu-mir-466i, mmu-mir-669i, mmu-mir-669h, mmu-mir-466f-4, mmu-mir-466k, mmu-mir-466j, mmu-mir-1193, mmu-mir-767, rno-mir-362, rno-mir-504, rno-mir-582, rno-mir-615, mmu-mir-3080, mmu-mir-466m, mmu-mir-466o, mmu-mir-466c-2, mmu-mir-466b-4, mmu-mir-466b-5, mmu-mir-466b-6, mmu-mir-466b-7, mmu-mir-466p, mmu-mir-466n, mmu-mir-344e, mmu-mir-344b, mmu-mir-344c, mmu-mir-344g, mmu-mir-344f, mmu-mir-374c, mmu-mir-466b-8, hsa-mir-466, hsa-mir-1193, rno-mir-449c, rno-mir-344b-2, rno-mir-466d, rno-mir-344a-2, rno-mir-1193, rno-mir-344b-1, hsa-mir-374c, hsa-mir-499b, mmu-mir-466q, mmu-mir-344h-1, mmu-mir-344h-2, mmu-mir-344i, rno-mir-344i, rno-mir-344g, mmu-let-7j, mmu-mir-30f, mmu-let-7k, mmu-mir-692-3, rno-let-7g, rno-mir-15a, rno-mir-762, mmu-mir-466c-3, rno-mir-29c-2, rno-mir-29b-3, rno-mir-344b-3, rno-mir-466b-3, rno-mir-466b-4
Of these miRNAs, 12 were upregulated (miR-34b, miR-138, miR-297a, miR-301, miR-449, miR-466, miR-493, miR-579, miR-582, miR. [score:4]
1Proliferation, Invasion, Tumor suppression [63– 66] miR-344 ↓2.0 ↓3.2 NA miR-346 ↓2.4Proliferation [67, 68] miR-362 ↓2.3Proliferation, Invasion, Apoptosis [69– 76] miR-369 ↓2.8 ↓2.6 ↓2.1Aerobic glycolysis [77] miR-374 ↑3.0 ↓2.2 NA miR-449 ↑2.7 ↑2.4Proliferation [78– 81] miR-463 ↓2.7 NAmiR-466 [°] ↑2.4 ↑2.1 ↓3.5 NA miR-483 ↓3.2Apoptosis [82] miR-493 ↑2.1 ↓2.2Proliferation [83– 85] miR-499a ↓5.0 ↑2.3Proliferation [86] miR-504 ↓2.6 ↑2.0Proliferation, Apoptosis [87, 88] miR-579 ↑2.8 NAmiR-582 [^] ↑2.4Proliferation [89] miR-615 ↓2.1Proliferation, Invasion [90, 91] miR-652 ↑2.4Proliferation, EMT [92, 93] miR-669b ↓2.1 NA miR-669h ↓3.6 ↑2.3 NA miR-669i ↓2.3 NA miR-669k ↓7.2 ↓5. [score:3]
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7
[+] score: 5
Uematsu et al. [32] reported that regulating the expression of SRY-box 4 (SOX4) and lymphoid enhancer -binding factor 1 (LEF1) by miR-29b-1-5p and miR-449a-5p are important in the development of Th2 bias in methimazole -induced liver injury. [score:5]
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8
[+] score: 4
They also showed the up-regulation of miR-1, miR-449a and a 60-fold induction of miR-135b. [score:4]
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9
[+] score: 4
rno-miR-881, rno-miR-880, miR-741-3p, miR-511*, miR-187, miR-449a, as well as 6 members of miR-34 family, miR-34a, miR-34a*, miR-34b, miR-34b*, miR-34c, and miR-34c*, showed over 10-fold up-regulation. [score:4]
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10
[+] score: 3
CAN-08-2886 19155302 8. Noonan EJ miR-449a targets HDAC-1 and induces growth arrest in prostate cancerOncogene. [score:3]
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11
[+] score: 3
Expression of miR-34a-5p, miR-34c-5p, miR-124-3p, and miR-150-5p (respectively, 52%, 56%, 47% and 20% lower than CTL; P < 0.05) but not miR-449a was reduced in the liver of 60-hour fasted rats born to DEX -treated mothers (Fig.   5G). [score:3]
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12
[+] score: 3
Some of these miRNAs, i. e. rno-miR-34c, rno-miR-449a, rno-miR-301b, rno-miR-532-5p, rno-miR-219-5p, rno-miR-451, and rno-miR-152, were even 10-fold more abundant at E10 than at any other stages, providing a hint that these 7 miRNAs may play important roles in the regulation of progenitor cell proliferation. [score:2]
In contrast, several E10-enriched miRNAs identified in our study, including rno-miR-181a, rno-miR-449a, and rno-miR-503, were not detected in their results. [score:1]
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13
[+] score: 2
This information prompted us to investigate whether the anti-apoptotic effects of lncRNA-XIST knockdown were exerted through miR-449 -mediated PTEN expression. [score:2]
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14
[+] score: 1
Other miRNAs from this paper: hsa-mir-449a, hsa-mir-582, hsa-mir-449c, rno-mir-582, rno-mir-449c
Emca3 also harbors several genes that encode small RNAs, including miR449a, miR449c, and miR582 (Table S3). [score:1]
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