sort by

6 publications mentioning dre-mir-137-1

Open access articles that are associated with the species Danio rerio and mention the gene name mir-137-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 244
This was achieved by the modulation of miR-137 expression in the zebrafish embryos throughout early development by both (i) direct administration of synthetic miR-137 mimic or morpholino (MO) miR-137 antagonist, and (ii) inducible transgenic expression of miR-137 or transgenic anti-miR137 sponge -RNA expression. [score:9]
While upregulation produced no observable specific phenotype, both transgenic miR-sponge and MO -induced downregulation of miR-137 inhibited touch–response behavior at embryonic and larval stages without modifying other swimming behaviors. [score:9]
Considering that inhibition of miR-137 inhibits the response of zebrafish embryos and larvae to mechanical stimuli, and that this miRNA is expressed in sensory neurons, we hypothesized that miR-137 might be involved in the early development of these cell types. [score:8]
[34] The miR-137 inhibitor construct (Figure 1a) constitutively expressed miR137-target sequence repeats (miR-sponges or SP137) in a fusion mRNA encoding the fluorescent marker protein mCherry. [score:7]
To confirm expression and activity of both agonist and antagonist systems, βactin:mCherry:10 × SP137 embryos expressing homogeneously and ubiquitously mCherry and SP137 were injected with both 503UNC:Gal4 (expressing Gal4 in muscle cells) [50] and UAS:YFPs:miR137 (Figure 1c). [score:7]
To further validate the phenotypic changes observed by direct chemical manipulation of miR-137 in vivo, we also introduced transgene constructs into the zebrafish genome designed either to block miR-137 activity by competing with its endogenous targets (Figure 1a) or to overexpress this small molecule (Figure 1b). [score:6]
It is also possible that its inhibition would modify the synaptic functionality of these cells through the regulation of signal transduction pathways, neurotransmitters and/or ion channels, which have been implicated in bioinformatics and functional screening of miR-137 target genes. [score:6]
Synthetic-miR137 expression correlated with downregulation of mCherry fluorescent intensity, confirming efficient activity of both the sponges and the transgenic synthetic-miR137 (Figure 1c). [score:6]
[37] The synthetic-miR137-inducible plasmid was constructed by cloning the human pri-miR downstream of the fluorescent marker YFP and under the control of the UAS promoter, thus allowing inducible expression of both YFP and miR-137 in tissue(s) expressing the Gal4 protein (Figure 3b). [score:5]
After injection of 8 ng (or higher) of MO137-02, miR-137 expression was no longer detectable even after extended revelation, suggesting potent inhibition (Figure 3i and j). [score:5]
We induced expression in muscles as this tissue does not express endogenous miR-137, which would have interfered with our readout. [score:5]
We crossed βactin:mCherry:10 × SP137 animals with a SEN:GFP zebrafish line that expresses GFP in, but not limited to, sensory neurons including Rohon–Beard (RB), dorsal root ganglia (DRG) and trigeminal neurons (TR), as well as non-sensory neurons Mauthner cells (M); cell types that were found to express endogenous miR-137 by in situ hybridization. [score:5]
To further support these observations without the associated off-target effects of oligonucleotide -based manipulation, we established a transgenic miR-137 suppression system that worked through the production of a stable miR-137 sponge transcript. [score:5]
However, as presented in Figure 3c, MO137-01 induced strong defects and premature death even down to 4 ng that appeared to be most likely due to off-target effects, thus precluding its application in further experiments as there is no relevant dose that could lead to potent miR137 inhibition without inducing off-specific morphological defects. [score:5]
Mosaic expression of synthetic-miR137 induced by presence of Gal4 can be tracked by the co -expression of the YFP protein (fluorescence processed in green in Figure 1c). [score:5]
In untreated animals, miR-137 is mainly expressed in the nervous system of zebrafish at 3 days post fertilization (dpf), including strong expression in sensory neurons (Supplementary Figure 1). [score:5]
By binding to SP137, endogenous miR-137 should repress mCherry translation, thus reducing red fluorescence where this miRNA is expressed. [score:5]
These observations suggest that miR-137 knockdown inhibits the touch response of zebrafish embryos and larvae without affecting RB, DRG, TR or M cell differentiation or their axonal projections. [score:4]
To validate the efficiency of MO -mediated downregulation of endogenous miR-137, we analyzed control versus MO -treated animals for the presence of mature miR-137 using in situ hybridization. [score:4]
However, we found that transient miR-137 knockdown inhibited the touch-evoked escape response of zebrafish larvae (Figure 4). [score:4]
One of these molecules, MO137-02, was able to inhibit miR-137 with no obvious side-effects during early development. [score:4]
Indeed, embryos expressing anti-miR137 sponges were significantly more sensitive to MO -mediated miR137 knockdown from 2 to 4 dpf. [score:4]
It is noteworthy that co-injection of either 75  or 25 pg of synthetic miR-137, as described below, failed to rescue these presumed off-target defects observed in embryos treated with 8 ng of MO137-01. [score:3]
These observations demonstrate that endogenous miR-137 is specifically expressed and functional in RB, M and DRG cells, and that MO137-02 efficiently reduces its activity. [score:3]
Finally, we did not record obvious abnormal death ratios in βactin:mCherry:10 × SP137 embryos, while transgenic pan-neuronal expression of human miR137 reduced the number of death events in MO137-02 (16 ng) -injected embryos between 6 and 7 dpf (Supplementary Figure 4). [score:3]
Importantly we were able to partially rescue this phenotype by co-administration of synthetic miR-137 and by transgenic pan-neuronal expression of miR-137. [score:3]
The F1 line that led to the strongest YFP expression (thus of synthetic miR-137) in the nervous system was selected and used in this study. [score:3]
Embryos were injected with either MO137-02 or MO-control, and sorted at 28 hpf for the presence or absence of YFP in the nervous system; a marker for the presence or absence of transgenic miR-137 expression. [score:3]
We also took advantage of the βactin:mCherry:10 × SP137 transgenic line, which expresses mCherry:10 × SP137 RNA ubiquitously, to (i) validate SP137 affinity for endogenous miR-137, (ii) confirm presence and activity of endogenous miR-137 in specific cell types and (iii) validate MO efficiency in these tissues. [score:3]
For this purpose, we designed several MOs and tested both their capacity to suppress miR-137 maturation and their toxicity. [score:3]
[57] Interestingly, zebrafish present two copies of the syt1 gene, with both having two putative miR-137 sites in their 3′ untranslated region. [score:3]
Validation of miR-137 inhibition. [score:3]
However, it is possible that miR-137 inhibition would lead to subtle histological changes not detected in this study. [score:3]
MiR-137 suppression was achieved with two MOs, designated MO137-01 and MO-137-02, which were designed to interfere with miR-137 maturation by masking Drosha cleavage sites (Figure 3a, Supplementary Table 1). [score:3]
Transgenic miR-137 is still expressed at 4 dpf though. [score:3]
It is noteworthy that transgenic pan-neuronal expression of miR-137 increases the response of the fish in all conditions tested except at 28 hpf. [score:3]
We found that miR-137 in zebrafish is expressed and active in the sensory neurons responsible for touch-sensitivity of zebrafish embryos and larvae. [score:3]
This synergistic effect observed between Sp137 and MO137-02 supports the association of miR-137 suppression with decreased touch-sensitivity in zebrafish embryos and larvae. [score:3]
For transient miR-137 overexpression, we used a synthetic version of mature miR-137, designated miR-137 mimic (Supplementary Table 1). [score:3]
[49] Human pri-miR137 was cloned instead of zebrafish pri-miR because of the three mismatches in the loop sequence, making it less sensitive to MO -inhibition and thus improving potential rescue experiments. [score:3]
Adults were outcrossed with wild types to identify F0 founders that give birth to embryos with strong red fluorescent expression, thus of anti-miR137 sponges. [score:3]
In addition to having the potential to block miR-137 activity by competing with its endogenous targets, mCherry:10 × SP137 RNA can be used as a sensor of miR-137 activity. [score:3]
The miR-137 expression signal started to return in animals treated with only 4 ng. [score:3]
Significantly, this experiment supported the role for miR-137 in touch-sensitivity observed in the transient assays, as transgenic expression of miR-137 in neurons was sufficient to partially rescue the touch-insensitivity of MO137-02 injected embryos (Figure 2b). [score:2]
To transiently manipulate miR-137 activity in zebrafish, we utilized direct delivery of synthetic miR-137 oligonucleotide antagonists and mimics. [score:2]
More recently, miR-137 has also been shown to modify presynaptic function though its regulation of Syt1 in the mouse. [score:2]
In conclusion, we find that miR-137 knockdown reduces zebrafish touch–response behavior. [score:2]
[15] These studies strongly implicate the MIR137 locus in the susceptibility for schizophrenia and suggest that the encoded miRNA may be involved in the disorder through a role in shaping neurodevelopment. [score:2]
Interestingly, at high dose (16 ng) of this MO, we also saw significant lethality that we initially attributed to non-specific toxicity; however, in view of the recent observation of embryonic lethality in the miR-137 homozygous knockout mouse [53] it is possible that this dose was ablating miR-137 function and inducing death. [score:2]
Finally, considering the recent advance of genome editing tools, an interesting option would also be to knockout miR-137. [score:2]
1, 16, 17, 18, 19 MiR-137 is highly expressed in the brain 20, 21 and has been associated with neuronal differentiation, maturation and transmission, dendritic spine morphogenesis and synaptogenesis. [score:2]
miR-137 knockdown does not induce obvious anatomical defects of the sensory neurons. [score:2]
In contrast, the miR137 -mimic was present from one-cell stage but may lose its effect at 4 dpf because of breakdown and dilution during development of the growing animal. [score:2]
On the contrary, all doses of the miR137 -mimics (with the exception of 25 pg) induced strong abnormalities, including growth retardation, small eyes, heart edema and curved tail. [score:1]
We co -injected miR137 -mimics or miRNA control (miR-CTR) with MO137-02 or MO-control (Figure 4c). [score:1]
It is noteworthy that the alternative miR-137 MOs, MO137-01, at ⩾4 ng led to a reduction of miR-137 in situ signal, which was no more obvious at a dosage of ⩽2 ng. [score:1]
Synthetic hsa-pri-miR137 was then inserted in 107-UAS-YFPs-Gtwy-clmc2CHERRY via LR clonase reaction. [score:1]
It is probable that miR-137 function, which modulates zebrafish touch–response phenotype, would act through the same mechanisms in other neuronal population(s) to lead to pain- and olfactory-deficits. [score:1]
[45] These anti-miRs were in theory capable of neutralizing the entire dre-miR-137 family as all three dre-miR-137 genes in the zebrafish genome share the exact same pre-miR sequence (Figure 3a). [score:1]
All MOs and synthetic miRNA sequences used in this study are listed in Supplementary Table 1. miR-430 mimics was obtained from Ambion (product ID MC10393) and was co -injected along with miR-137 mimics at 100 pg. [score:1]
Indeed, miR-137 is conserved between zebrafish and human, thus its molecular role should have many parallels even if its physiological role may have diverged to some extent. [score:1]
As these adverse effects were not observed at 25 pg, we considered this dosage safe for rescuing MO -mediated miR-137 knockdown phenotype in the following experiments. [score:1]
p3E-10 × SP137 contains 10 × anti-miR137 sponge sequences that were synthetized by Biobasic (https://store. [score:1]
107-UAS-YFPs-miR137-clmc2CHERRY was injected in one-cell stage, wild-type embryos using 2 nl of a mixture containing 25 ng μl [−1] of DNA construct along with 20 μg μl [−1] of transposase RNA. [score:1]
Transgenic lines were generated and named, respectively, βactin:mCherry:10 × SP137 for the sponge construct and UAS:YFPs:miR137 for the inducible plasmid. [score:1]
Mo del systems capable of revealing cellular, neuroanatomical and neurobehavioural dimensions of miR-137 will be vital to galvanize the emerging evidence for a role in neuropsychiatric and neurocognitive disorders. [score:1]
Among these discoveries, the gene for the small non-coding RNA miR-137 was implicated with the second highest association (P<10 [−17]). [score:1]
Synthetic miR-137 construct and transgenic lines. [score:1]
This premature death was not rescued by co-injection of miR-137 mimics, which is not surprising given that these molecules are only likely to remain effective for a few days following injection (Supplementary Figure 4). [score:1]
Anti-miR137 sponge (SP137) construct and transgenic lines. [score:1]
miR-137 LNA probe was labeled with 5′-DIG and 3′ DIG (reference 35112–15). [score:1]
It was named UAS:YFPs:137. miRCURY miR-137 LNA probe for miRNA detection was obtained from Exiqon and prepared following the manufacturer's instruction (http://www. [score:1]
We first tested anti-miR137 MOs in zebrafish by injecting a range of doses. [score:1]
Transient manipulation of miR-137 activity. [score:1]
[55] For instance, the l-type voltage -dependent calcium channel CACNAC1, which was recently associated with SCZ, 15, 56 is present in zebrafish with a transcript predicted to have two putative sites for miR-137. [score:1]
As seen in Figure 1d, due to the presence of miR-137, GFP -positive RB cells presented weak red fluorescence, which was increased dramatically in the presence of 8 ng of MO137-02. [score:1]
miR-137 modulated sensorimotor phenotype in zebrafish larvae. [score:1]
It is noteworthy that the mature miR-137 is fully conserved between zebrafish and mammals including humans, with hsa-pre-miR137 presenting only three mismatches in the pre-miR loop sequence (Figure 3a). [score:1]
Anti-miR-137 sponge reduces zebrafish touch–response. [score:1]
We injected miR137 -mimics and miR-Control in the dose range of 25–150  pg (Figure 3b, f and g). [score:1]
[47] To test this hypothesis, we co -injected miR-137 -mimics along with miR-430 mimics (Figure 3h). [score:1]
[25] While miR-137 seems to have important diverse biological functions, particularly in the brain, this has not yet been fully elaborated in molecular or genetic intervention studies in vivo. [score:1]
Our first hypothesis was that miR-137 would impact such cellular mechanisms in sensory neurons thus delaying touch–response behavior. [score:1]
To validate these results, we attempted to rescue the MO137-02 -induced touch–response phenotype by introducing synthetic miR-137. [score:1]
Transgenic manipulation of miR-137 activity. [score:1]
[63] This suggests that the sensory miR137 -dependent phenotype observed in zebrafish is potentially of high interest to bring new insight into schizophrenia biology. [score:1]
com/gene-synthesis/), recognizing both zebrafish and human mature miR-137 (Supplementary Figure 6). [score:1]
[54] However, this strategy would require considerable effort, as three different copies of the miR-137 gene exist in zebrafish. [score:1]
34, 48 The sponges were designed to present a bulge at position 9–12 of miR-137 to maximize antagonistic effect without inducing RISC -associated RNA-cleavage of the mCherry:SP137 mRNA (Figure 1a). [score:1]
In situ hybridizationmiRCURY miR-137 LNA probe for miRNA detection was obtained from Exiqon and prepared following the manufacturer's instruction (http://www. [score:1]
miR-137 mimics was ordered from Sigma-Aldrich (Sigma Genosys, USA, miR-137 duplex) and miR-Control was mirVana miRNA mimic Negative Control #1 from Applied Biosystems (Cat# 4464059), both were dissolved following manufacturer's instructions. [score:1]
[1 to 20 of 91 sentences]
2
[+] score: 34
Other miRNAs from this paper: dre-mir-7b, dre-mir-7a-1, dre-mir-7a-2, dre-mir-34a, dre-mir-181b-1, dre-mir-181b-2, dre-mir-182, dre-mir-183, dre-mir-181a-1, dre-mir-219-1, dre-mir-219-2, dre-mir-221, dre-mir-222a, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-7a-3, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-92b, dre-mir-96, dre-mir-100-1, dre-mir-100-2, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-125b-1, dre-mir-125b-2, dre-mir-125b-3, dre-mir-128-1, dre-mir-128-2, dre-mir-132-1, dre-mir-132-2, dre-mir-135c-1, dre-mir-135c-2, dre-mir-137-2, dre-mir-138-1, dre-mir-153a, dre-mir-181c, dre-mir-200a, dre-mir-218a-1, dre-mir-218a-2, dre-mir-219-3, dre-mir-375-1, dre-mir-375-2, dre-mir-454a, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-2, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j, dre-mir-181a-2, dre-mir-34b, dre-mir-34c, dre-mir-222b, dre-mir-138-2, dre-mir-181a-4, dre-mir-181a-3, dre-mir-181a-5, dre-mir-181b-3, dre-mir-181d, dre-mir-128-3
miR-137 is expressed in the adult midbrain DTN (Figure 3g) and given the good correspondence of localized tegmental expression between 5 dpf and adult brains, we suggest that the cells in the larval tegmentum correspond to the presumptive midbrain DTN (Figure 3e,g; for other nuclei, see0, and Tables C and H in7). [score:5]
In contrast to the miRNAs that are broadly expressed in proliferative or differentiated CNS cells, many others, including miR-128 and miR-137, have larval expression restricted to specific brain areas/nuclei (Additional data files 8 and 10, and Table C in7). [score:5]
Figure 3 miR-137 and miR-222 expression is conserved between larval and adult brain. [score:3]
Click here for file 0 miR-137 expression in the zebrafish brain. [score:3]
Additional data file 10 is a figure showing miR-137 expression in the zebrafish brain. [score:3]
For example, at 5 dpf, miR-137 expression is restricted to domains of the pallium (P in Figure 3a), dorsal thalamus (DT in Figure 3c), rostral and intermediate hypothalamus (Hr and Hi, respectively, in Figure 3c,f), ventral posterior tubercular area (PTv in Figure 3f) and specific nuclei in the tegmentum (midbrain dorsal tegmental nucleus (DTN in Figure 3e)). [score:3]
Like miR-137, miR-222 shows conserved restricted expression in the rostral brain throughout life with domains in the telencephalon (for instance, compare P in Figure 3h with P, Dm, Dd, Dl, and Dc in Figure 3i,j), hypothalamus (DIL, TLa, lr/Hd-lr in Figure 3k,l) and posterior tubercular area (PTv in Figure 3k and nPVO in Figure 3l; see also0, and Tables D and I in7). [score:3]
miR-137 expression in the zebrafish brain. [score:3]
miR-137 (Figure 5i) and miR-9 (Figure G in) show expression in groups of dorsal lateral habenular cells of the adult brain. [score:3]
For instance, miR-137 shows conserved expression in larval and adult brain in cells of the pallium (P in Figure 3a, and Dm, Dl, Dp in Figure 3b), dorsal thalamus (DT in Figure 3c and CP in Figure 3d), posterior tubercular area (PTv in Figure 3f and PTN in Figure 3d,g) and other areas (Additional data file 10). [score:3]
[1 to 20 of 10 sentences]
3
[+] score: 8
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, mmu-let-7g, mmu-let-7i, mmu-mir-124-3, mmu-mir-9-2, mmu-mir-134, mmu-mir-137, mmu-mir-138-2, mmu-mir-145a, mmu-mir-24-1, hsa-mir-192, mmu-mir-194-1, mmu-mir-200b, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-215, hsa-mir-221, hsa-mir-200b, mmu-mir-296, mmu-let-7d, mmu-mir-106b, hsa-let-7g, hsa-let-7i, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-137, hsa-mir-138-2, hsa-mir-145, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-134, hsa-mir-138-1, hsa-mir-194-1, mmu-mir-192, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-24-2, mmu-mir-346, hsa-mir-200c, mmu-mir-17, mmu-mir-25, mmu-mir-200c, mmu-mir-221, mmu-mir-124-1, mmu-mir-124-2, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-138-1, mmu-mir-7a-1, mmu-mir-7a-2, mmu-mir-7b, hsa-mir-194-2, mmu-mir-194-2, hsa-mir-106b, hsa-mir-200a, hsa-mir-296, hsa-mir-369, hsa-mir-346, mmu-mir-215, gga-let-7i, gga-let-7a-3, gga-let-7b, gga-let-7c, gga-mir-221, gga-mir-17, gga-mir-138-1, gga-mir-124a, gga-mir-194, gga-mir-215, gga-mir-137, gga-mir-7-2, gga-mir-138-2, gga-let-7g, gga-let-7d, gga-let-7f, gga-let-7a-1, gga-mir-200a, gga-mir-200b, gga-mir-124b, gga-let-7a-2, gga-let-7j, gga-let-7k, gga-mir-7-3, gga-mir-7-1, gga-mir-24, gga-mir-7b, gga-mir-9-2, dre-mir-7b, dre-mir-7a-1, dre-mir-7a-2, dre-mir-192, dre-mir-221, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-7a-3, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-17a-1, dre-mir-17a-2, dre-mir-24-4, dre-mir-24-2, dre-mir-24-3, dre-mir-24-1, dre-mir-25, dre-mir-92b, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-137-2, dre-mir-138-1, dre-mir-145, dre-mir-194a, dre-mir-194b, dre-mir-200a, dre-mir-200b, dre-mir-200c, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-2, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, mmu-mir-470, hsa-mir-485, hsa-mir-496, dre-let-7j, mmu-mir-485, mmu-mir-543, mmu-mir-369, hsa-mir-92b, gga-mir-9-1, hsa-mir-671, mmu-mir-671, mmu-mir-496a, mmu-mir-92b, hsa-mir-543, gga-mir-124a-2, mmu-mir-145b, mmu-let-7j, mmu-mir-496b, mmu-let-7k, gga-mir-124c, gga-mir-9-3, gga-mir-145, dre-mir-138-2, dre-mir-24b, gga-mir-9-4, mmu-mir-9b-2, mmu-mir-124b, mmu-mir-9b-1, mmu-mir-9b-3, gga-mir-9b-1, gga-let-7l-1, gga-let-7l-2, gga-mir-9b-2
MiR-137 functions as a tumor suppressor in neuroblastoma by downregulating KDM1A. [score:5]
miR-137. [score:1]
Epigenetics, microRNAs, and carcinogenesis: functional role of microRNA-137 in uveal melanoma. [score:1]
When an anti-miR-137 is used, Jarid1b is not post-transcriptionally silenced and the differentiation of ESCs is blocked (Tarantino et al., 2010). [score:1]
[1 to 20 of 4 sentences]
4
[+] score: 5
The expression level of miR-137, miR-724, miR-7a and miR-734 was more than 10 fold higher in silver than in bighead carp, while for miR-196b, the expression level was more than 10 fold higher in bighead carp than in silver carp. [score:5]
[1 to 20 of 1 sentences]
5
[+] score: 5
Notably, cardiac-specific miR-1, miR-133, miR-208 and miR-499 were all suppressed by two or more orders of magnitude [34], [35], as were the stemness and cell cycle repressors miR-141 and miR-137 [36]; in contrast, the proliferative miRNAs, miR-222 [37], increased dramatically in MDCs, and miR-221 was undetectable in myocytes but highly expressed in MDCs (Figure 5D). [score:5]
[1 to 20 of 1 sentences]
6
[+] score: 3
However, the expression levels of miR-124-3p, miR-124-5p, miR-124-6-5p, miR-133a-3p, miR-133b-3p, miR-135b-3p, miR-135b-5p, miR-137-3p and some other miRNAs were rather low (Excel S1). [score:3]
[1 to 20 of 1 sentences]