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1 publications mentioning ptr-mir-184

Open access articles that are associated with the species Pan troglodytes and mention the gene name mir-184. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 40
To assess whether miRNA with expression divergence on the human lineage might be associated with human cognitive functions, we investigated the expression of genes targeted by five miRNA showing human-specific expression, according to multiple methodologies: miR-184, miR-487a, miR-383, miR-34c-5p and miR-299-3p (Figure 2). [score:7]
Potential influence of miRNA divergence on neuronal functions was further confirmed by preferential expression of the corresponding miR-299-3p and miR-184 in cortical neurons, as well as verification of the predicted miRNA-target relationship in two human neuroblastoma cell lines. [score:5]
This effect was significant for combined targets of the five miRNA, as well as for targets of miR-184 and miR-383 analyzed individually. [score:5]
Furthermore, miR-184 targets were significantly enriched in “long-term potentiation” pathway – one of the few pathways directly connected to learning and memory formation [34], [35]. [score:4]
Similarly, based on the DIANA-mirPath algorithm [33], targets of miR-184, miR-487a and miR-299-3p were significantly enriched in KEGG pathways that are related to neural functions (Table S10). [score:3]
To further test association of miR-184 and miR-299-3p with neuronal functions, we determined their expression patterns in the human and macaque prefrontal cortex by in situ hybridization with specific LNA-probes (Table S11). [score:3]
Based on the DIANA-mirPath algorithm, targets of miR-184, miR-487a and miR-299-3p were significantly enriched in KEGG pathways that are related to neural functions (Table S10). [score:3]
Requiring significant support by at least two out of three methodologies (sequencing, microarrays and Q-PCR), expression changes in five miRNA (miR-184, miR-299-3p, miR-487a, miR-383 and miR-34c-5p) could be assigned to the human evolutionary lineage and two (miR-375 and miR-154*) to the chimpanzee evolutionary lineage (Figure 2). [score:3]
Recent studies have also shown that miR-184 is involved in regulation of neural stem cell proliferation and differentiation [36]. [score:2]
We chose three types of miRNA differences: (1) consistent by both methodologies: miR-383 and miR-34c-5p; (2) significant according to sequencing, but unconfirmed in the microarray experiment: miR-143 and miR-499; (3) significant according to sequencing, but not detected or masked on the microarrays: miR-184 and miR-299-3p. [score:1]
1002327.g004 Figure 4 In situ staining of miR-184 and miR-299-3p in prefrontal cortex. [score:1]
In situ staining of miR-184 and miR-299-3p in prefrontal cortex. [score:1]
We designed two LNA-probes complementary to miR-184 and miR-299-3p respectively (Table S11). [score:1]
On the DNA sequence level, these miRNA tend to be conserved: miR-184 mature miRNA sequence is evolutionarily conserved from insects to humans, with only one nucleotide different at 3′end of mature sequence, while miR-383 and miR-34c-5p are classified as broadly conserved and miR-299-3p - as conserved among animal species [25], [31]. [score:1]
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