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1 publications mentioning mml-mir-25

Open access articles that are associated with the species Macaca mulatta and mention the gene name mir-25. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 54
The results showed that 14 miRNAs (miR-30a-5p, miR-30e-5p, miR-425-5p, miR-142-3p, miR-191a-3p, miR-215, miR-29b-3p, miR-30b-5p, miR-26a-5p, miR-345-5p, miR-361-5p, miR-185-5p, miR-103-3p) were down-regulated but no miRNA was up-regulated among above three altered miRNAs from microarray in OVX serum by normalizing to miR-25-3p (Fig. 3b). [score:7]
In present, we verified miR-25-3p as a stable reference endogenous control gene and 4 downregulated biomarkers from women with normal BMD, osteopenia and osteoporosis. [score:4]
In addition, miR-328-3p also showed a notable decrease in osteoporotic patients in our study by normalizing to miR-25-3p (Fig. 4a), which was reported down-regulation in the serum of postmenopausal osteoporotic fracture and involved in bone formation by Weilner et al. 13. [score:4]
The expression of miR-25-3p was also detected in the head down bedrest monkeys with osteoporosis due to mechanical unloading and showed no significant difference from that in the control groups (Fig. 2e). [score:3]
Therefore, the consensus of the unchanging expression signature of miR-25-3p in serum proved that miR-25-3p should be a suitable reference gene of serum miRNAs for osteoporosis. [score:3]
Moreover, our results conformed the finding of Seeliger et al. 12 who reported no differential expression of miR-25-3p in serum between non-osteoporotic and osteoporotic fracture patients. [score:3]
Similarly, no significant change of serum miR-25-3p expression was found among postmenopausal women with normal BMD, osteopenia or osteoporosis (Table 1 and Fig. 2f). [score:3]
showed that there were no significant differences of miR-25-3p expression in those paired subjects. [score:3]
Serum was collected from different stage at 1 [st] to 6 [th] week and the expression levels of 4 top ranked miRNAs (miR-25-3p, miR-140-5p, miR-342-5p and miR-150-5p) were assayed by qPCR. [score:2]
The qPCR results of miR-25-3p expression showed no marked change compared with their control (Fig. 2i,j). [score:2]
Identified miR-25-3p as the suitable reference gene for osteoporosis. [score:1]
Therefore, ten miRNAs (miR-19b-3p, miR-21-5p, miR-25-3p, miR-30a-5p, miR-133b-3p, miR-140-5p, miR-150-5p, miR-199a-3p, miR-342-5p, miR-3473) were chosen as candidate reference genes. [score:1]
In addition, the expression pattern of miR-25-3p during osteoclast and osteoblast differentiation was investigated in vitro. [score:1]
miR-25-3p was also detected in rhesus monkeys after long-duration bedrest and postmenopausal women with osteoporosis. [score:1]
miR-25-3p was identified as the most suitable reference gene and expanded its application scope to both estrogen deficiency- and mechanical unloading- induced osteoporosis. [score:1]
Ssa-miR-25-3p was identified as the most suitable single reference gene in Atlantic salmon 29. [score:1]
Given this, miR-25-3p was detected in the femur of HU and OVX rats (Fig. 2gh). [score:1]
Recently, Das et al. reported miR-25-3p was the most suitable reference gene in human cancer cell lines and even more stable than RNU6 28. [score:1]
miR-25-3p was recommended as the most suitable reference gene according to the ranking of 9 candidate miRNAs by two statistical algorithms (geNorm and NormFinder). [score:1]
Obviously, miR-25-3p was rather stable in both algorithms, and was recommended as reference gene by comprehensive ranking. [score:1]
Conversely, miR-140-5p, miR-342-5p and miR-150-5p were not as stable as miR-25-3p and showed some notable changes at some stage (Fig. 2c). [score:1]
During HU within 6 weeks, miR-25-3p was the most stable gene and showed no significant difference between any pair matched groups (pā€‰>ā€‰0.05). [score:1]
With this algorithm, miR-140-5p was identified as the most stable gene, followed by miR-25-3p (Fig. 2b). [score:1]
The stability of miR-25-3p was further validated at different stage of HU osteoporotic mo dels in another independent experiment. [score:1]
To extend its application scope, we further estimated the stability of miR-25-3p in various osteoporosis mo dels and species. [score:1]
Indeed, miR-25-3p showed no marked alterations in the femur of OVX or HU rats, as well as during osteogenic or osteoclastic differentiation. [score:1]
By geNorm, miR-25-3p was ranked as the most stable gene and a combination of miR-25-3p, miR-342-5p and miR-140-5p was recommended for optimal normalization (Fig. 2a). [score:1]
To examine whether miR-25-3p is also stable in some extended osteoporotic mo dels, including OVX rats, monkey after head down bedrest and postmenopausal women, serum was collected from each osteoporotic mo del. [score:1]
All these results confirmed miR-25-3p was a suitable reference gene of serum miRNAs for osteoporosis. [score:1]
Validation of miR-25-3p as suitable reference gene in osteoporotic mo dels. [score:1]
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