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34 publications mentioning hsa-mir-490

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-490. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 160
Studies reported that miR-490 inhibits bladder cancer proliferation by targeting c-Fos [27], miR-490-3p could directly target nanog in mouse embryonic stem cell [29], and miR-490-3p exert suppressor in growth and metastasis on gastric cancer cells through directly targeting SMARCD1 [24]. [score:13]
After overexpressed of miR-490-3p in endometrial cancer cells the expression of TGFα was decreased, while EGFR and the downstream related genes including NF-kB, MMP-2, Cyclin D1, survivin were decreased in mRNA and protein level, while increased Bax expression. [score:7]
So the anti-oncogene role of miR-490-3p in endometrial carcinoma may downregulating the expression of c-Fos. [score:6]
Besides, in vivo nude mice xenograft assays, the overexpression of miR-490-3p significantly inhibited tumor growth, these results all suggest that miR-490-3P may inhibit endometrial carcinoma tumorigenesis and progression. [score:6]
Figure 5The bioinformatic software predicted that the 3′ UTR of TGFα was a direct target of miR-490-3p (A)A dual-luciferase reporter assay indicated that miR-490-3p directly targeted TGFα by binding its 3′ UTR (* P < 0.05, B). [score:6]
Figure 7Compared with the mock control, nude mice treated with miR-490-3p showed a dramatic reduction in tumor size (A) and tumor xenograft growth from day 4 and week 2 onwards (* P < 0.05, B), while the deviation of tumor xenograft volume [DV] increased in the latter period (* P < 0.05, C) MiR-490-3p downregulated TGFα expression in tumor xenografts in vivoImmunohistochemistry (IHC) and Western Blotting indicated that TGFα expression in the tumor xenografts of hsa-miR-490-3p -treated nude mouse was decreased compared with that in mock nude mice (Figure 8A-8B). [score:6]
In conclusion, miR-490-3p has a tumor suppressor role in endometrial cancer and with c-Fos and TGFα as direct target gene. [score:6]
The expression of miR-490-3p increased after transfection and the ectopic expression of miR-490-3p inhibited cell proliferation in an MTT assay. [score:6]
Above all, these results suggest that miR-490-3P may inhibit endometrial cancer tumorigenesis and progression through targeting TGFα. [score:5]
The expression of miR-490-3p was significantly downregulated in EC samples compared with normal samples (Figure 1A, p < 0.05), and was negatively associated with depth of invasion (mucosa vs. [score:5]
Our bioinformatics software prediction showed that miR-490-3p has a direct target in the 3′ UTR of TGFα, our dual luciferase reporter assays, the mRNA and protein expression levels of TGFα in miR-490-3p -transfected cells and in nude mice the tumor tissues of Hsa-490-3p group were decreased all verified this prediction. [score:5]
Besides, we found that miR-490-3P overexpression target TGFα in endometrial carcinoma. [score:5]
Our software predicted that miR-490-3p could regulate the expression of TGFα via direct binding to its 3′ UTR. [score:5]
MiR-490-3p downregulated TGFα expression in tumor xenografts in vivo. [score:5]
The bioinformatic software predicted that the 3′ UTR of TGFα was a direct target of miR-490-3p (A). [score:4]
Effects of miR-490-3p transfection on EC cell genotype in vitroThe bioinformatic software predicted that miR-490-3p has a direct target in the 3′ UTR of TGFα (Figure 5A). [score:4]
The bioinformatic software predicted that miR-490-3p has a direct target in the 3′ UTR of TGFα (Figure 5A). [score:4]
In this study we aimed to investigate whether miR-490-3p is a suppressor in human EC and to identify the direct target associated with TGFα. [score:4]
We found that miR-490-3p was expressed at a lower level in EC than in normal tissues and was negatively associated with depth of invasion and lymph node metastasis, after treated with miR-490-3p mimics, EC cells showed decreased cell proliferation, migration and invasion ability, increased the percentage of cells in G1 phase and decreased those in S phase and promoted the apoptosis of endometrial carcinoma cells. [score:3]
To determine the effect of miR-490-3p overexpression in EC, we transfected endometrial cancer cell lines HEC-1B and Ishikawa with miR-490-3p -mimics. [score:3]
A dual-luciferase reporter assay indicated that miR-490-3p directly targeted TGFα by binding its 3′ UTR (* P < 0.05, B). [score:3]
However, miR-490-3p modulates cell growth and EMT in hepatocellular carcinoma cells by targeting ERGIC3 [28]. [score:3]
Figure 8 To examine the expression levels of miR-490-3p in endometrial cancer, reverse transcription polymerase chain reaction (RT-PCR) analysis was performed among all the tissues samples. [score:3]
To examine the expression levels of miR-490-3p in endometrial cancer, reverse transcription polymerase chain reaction (RT-PCR) analysis was performed among all the tissues samples. [score:3]
The dual-luciferase reporter assay indicated that miR-490-3p directly targeted TGFα by binding its 3′ UTR (Figure 5B). [score:3]
At the same time, we detect the expression level of c-Fos, nanog and SMARCD1 in the endometrial carcinoma cells transfected with miR-490-3p through RT-PCR and. [score:3]
Effects of miR-490-3p transfection on EC cell phenotype in vitroTo determine the effect of miR-490-3p overexpression in EC, we transfected endometrial cancer cell lines HEC-1B and Ishikawa with miR-490-3p -mimics. [score:3]
Figure 2Following miR-490-3p transfection, HEC-1B, Ishikawa cell lines exhibited significantly slower growth (* P < 0.05, A), showed G1 arrest (* P < 0.05, B)Results are representative of three separate experiments; data are expressed as the mean ± standard deviation, * P < 0.05. [score:3]
Reverse transcription (RT)-PCR and western blotting showed that miR-490-3p overexpression c-Fos decreased and nanog and SMARCD1 showed no significant differences. [score:3]
Then showed the change on TGFα, EGFR, NF-kB, Cyclin D1, Survivin, MMP2 and Bax in mRNA and protein level after miR-490-3p overexpression, si-TGFα and si-EGFR in EC cell lines. [score:3]
The expression of MiR-490-3p in EC. [score:2]
Figure 3After transfection with the miR-490-3p mimics, showed early apoptosis (* P < 0.05, A) compared with the control and mock -transfected cellsResults are representative of three separate experiments; data are expressed as the mean ± standard deviation, * P < 0.05. [score:2]
MiR-490-3p inhibited tumor growth in vivo. [score:2]
MiR-490-3P has been reported as a suppressor in various human cancers, including colorectal [24], gastric [25] lung [26] and bladder [27]; and as an oncogene in hepatocellular carcinoma [28]. [score:2]
MiR-490-3p inhibited tumor growth in vivoCompared with the mock control, nude mice treated with hsa-miR-490-3p showed a dramatic reduction in tumor size (Figure 7A, p < 0.05) and tumor xenograft growth from day 4 and week 2 onwards (Figure 7B, day 4 p < 0.05; deviation of tumor xenograft volume [DV] p < 0.01, and week 2 p < 0.05; DV p < 0.01), while the DV increased in the latter period. [score:2]
Meanwhile, this study suggests that miR-490-3p could be considered as a potential target for the treatment and management of endometrial cancer in the future. [score:2]
A group of mice (n = 10) received HEC-1B cells stably transfected with hsa-miRNA-490-3p. [score:1]
HEK293T Cells were grown to approximately 60% confluence in 24-well plates and co -transfected with TGFα 3′-UTR containing the putative miR-490-3p binding site or a mutant sequence designed based on the human TGFα mRNA sequence in GenBank and inserted into the downstream region of the firefly luciferase reporter (Promega, Madison, WI, USA). [score:1]
Many reports had showed the function of miR-490-3p in many cancers. [score:1]
Those results consistent with miR-490-3p in colorectal [24], gastric [25], lung [26] and bladder [27] cancer, in contrast with hepatocellular carcinoma. [score:1]
Effects of miR-490-3p transfection on EC cell phenotype in vitro. [score:1]
However, little is known about the biological functions of miR-490-3p in EC. [score:1]
Effects of miR-490-3p transfection on EC cell genotype in vitro. [score:1]
Figure 7Compared with the mock control, nude mice treated with miR-490-3p showed a dramatic reduction in tumor size (A) and tumor xenograft growth from day 4 and week 2 onwards (* P < 0.05, B), while the deviation of tumor xenograft volume [DV] increased in the latter period (* P < 0.05, C) (IHC) and Western Blotting indicated that TGFα expression in the tumor xenografts of hsa-miR-490-3p -treated nude mouse was decreased compared with that in mock nude mice (Figure 8A-8B). [score:1]
Following miR-490-3p transfection, HEC-1B, Ishikawa cell lines exhibited significantly slower growth (* P < 0.05, A), showed G1 arrest (* P < 0.05, B). [score:1]
MiR-490-3p mimics designed to mimic endogenous mature miR-490-3p (5′-CAA CCU GGA GGA CUC CAU GCU G-3′) were purchased from GenePharma (Shanghai, China), as well as scrambled oligonucleotides, which did not produce identifiable effects on miR-490-3p function, and were used as negative controls. [score:1]
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2
[+] score: 143
Moreover, we identified that miR-490-5p lentivirus partly rescued the expression of these markers, and the downregulation of BMPR2 by siRNA lentivirus had similar effects as the overexpression of miR-490-5p, which further indicates that miR-490-5p inhibits the process of chondrogenesis by targeting BMPR2. [score:12]
Of the 2 downregulated miRNAs, miR-490-5p was significantly downregulated in all 3 samples, and the expression of miR-1307 was down-regulated only in sample 2 (Fig. 3). [score:12]
In order to confirm the results of the microarray analysis, we conducted a northern blot analysis to detect the expression levels of 8 representative differentially expressed miRNAs identified using the microarray, including 4 upregulated miRNAs (miR-196a, miR-193b, miR-383 and miR-143) and 4 downregulated miRNAs (miR-490-5p, miR-1307, miR-125b and miR-590). [score:11]
The results revealed that the expression levels of SOX2 and BMPR2 in the hADSCs subjected to chondrogenic differentiation were upregulated, and the transfection of the cells with miR-490-5p lentivirus resulted in the downregulation of BMPR2 after the induction of hADSC differentiation, whereas the expression of SOX2 was not obviously affected (Fig. 5B). [score:11]
The miRNAs that exhibited at least a 2-fold change in expression in the hADSCs before and after the induction of chondrogenic differentiation are listed in Table I, and these include 12 upregulated miRNAs (miR-196a, miR-143, miR-383, miR-193b, let-7i, miR-26a, miR-539, miR-199a-3p, miR-337-5p, miR-146a-5p, miR-646, and miR-381) and 8 downregulated miRNAs (miR-490-5p, miR-1307, miR-125b, miR-96-3p, miR-302-3p, miR-23a-3p, miR-590, and miR-510). [score:9]
A northern blot analysis was conducted to confirm that miR-196a and miR-490-5p were indeed differentially expressed in all 3 samples, which is consistent with the microarray results, whereas miR-193b and miR-383 were found to be upregulated in only 2 samples, and miR-1307 was downregulated in only 1 sample. [score:9]
However, only 5 miRNAs [miR-196a, miR-193b, miR-383 (upregulated), miR-490-5p and miR-1307 (downregulated)] produced northern blot analysis signals. [score:7]
Thus, miR-490-5p inhibits hADSC differentiation by suppressing BMPR2 expression. [score:7]
Accompanying hADSC differentiation, the expression of miR-490-5p was gradually downregulated and transfection with miR-490-5p lentivirus reversed the differentiation ability of the hADSCs. [score:6]
In order to further confirm the results that miR-490-5p regulates the chondrogenic differentiation of hADSCs, we performed an ELISA for the quantitative measurement of chondrogenic differentiation markers (Col2A1, Col10A1 and aggrecan); the levels of these markers were gradually upregulated on days 12, 15, and 18; however, the levels of these markers were downregulated in the cells transfected with the miR-490-5p lentivirus (Fig. 4C). [score:6]
In conclusion, we discovered a set of miRNAs that are differentially expressed during the process of hADSC chondrogenic differentiation and confirmed that miR-490-5p has the potential to inhibit the chondrogenesis of hADSCs. [score:5]
The human BMP receptor type 2 (BMPR2) 3′ untranslated region (3′UTR) harboring the miR-490-5p target sequence and the seed sequence mutated version (BMPR2-3′UTR-mut) were synthesized by GenePharma (Shanghai, China) and then ligated after the luc ORF into the pMIR-REPORT luciferase vector (Ambion). [score:5]
Consistent with the effect of miR-490-5p, we used ELISA analysis and found that the levels of Col2A1, Col10A1 and aggrecan were downregulated on days 12, 15 and 18 in the cells transfected with BMPR2 siRNA lentivirus (Fig. 6C). [score:4]
As shown in Fig. 4A, the chondrogenic differentiation of the hADSCs resulted in the downregulation of miR-490-5p in a time -dependent manner. [score:4]
BMPR2 as a direct target gene of miR-490-5p. [score:4]
Therefore, we confirmed that BMPR2 is a direct target of miR-490-5p. [score:4]
Since miR-196a and miR-490-5p were significantly differentially expressed in all 3 samples, we conducted a functional analysis. [score:3]
Since we confirmed that miR-196a and miR-490-5p were overexpressed and downregualted, respectively, in all 3 samples, we conducted functional analysis to detect the role of the 2 miRNAs in the chondrogenic differentiation of hADSCs. [score:3]
Bioinformatics analysis identified SOX2 and BMPR2 as putative targets of miR-490-5p (Fig. 5A), which are related to chondrogenic differentiation. [score:3]
Thus, these results demonstrate that miR-490-5p inhibits the chondrogenic differentiation of hADSCs. [score:3]
Using a luciferase reporter system, miR-490-5p was proven to target the 3′UTR of BMPR2. [score:3]
identified SOX2 and BMPR2 as putative targets of miR-490-5p (Fig. 5A), which are related to chondrogenic differentiation. [score:3]
Our findings on the reduction in the expression of miR-490-5p were in line with those of a previous study (22). [score:3]
Furthermore, luciferase reporter assays confirmed that miR-490-5p suppressed the BMPR2 3′UTR luciferase activity by 40%, and the luciferase activity was completely reversed following transfection with mutated BMPR2 3′UTR (Fig. 5C). [score:2]
Effect of miR-490-5p on chondrogenic differentiation of hADSCs. [score:1]
However, we only found that miR-490-5p, but not miR-196a (data not shown), had an obvious effect on chondrogenic differentiation. [score:1]
However, we found that miR-490-5p, but not miR-196a (data not shown), had an obvious effect on chondrogenic differentiation. [score:1]
The probe sequences were as follows: miR-196a antisense, 5′-CCCAACAACATGAAACTACCTA-3′; miR-193b antisense, 5′-AGCGGGACTTTGAGGGCCAGTT-3′; miR-383 antisense, 5′-AGCCACAATCACCTTCTGATCT-3′; miR-490-5p antisense, 5′-ACCCACCTGGAGATCCATGG-3′; miR-1307 antisense, 5′-AGCCGGTCGAGGTCCGGTCGA-3′; and U6 antisense, 5′-GCCATGCTAATCTTCTCTGTATC-3′. [score:1]
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3
[+] score: 52
Other miRNAs from this paper: hsa-mir-21, hsa-mir-27a, hsa-mir-106a
The mRNA expression levels of MDR1, GST-π (p < 0.05) and protein expression levels of P-gp, GST-π were down-regulated after miR-490-3P transfection in comparison to mock and negative control cancer cells. [score:8]
Figure 2 miR-490-3P down-regulate MDR1/P-gp and GST-π expression. [score:6]
miR-490-3P down-regulate expression of MDR1/P-gp and GST-π. [score:6]
Studies reveal that miR-490-3P overexpression leads to inhibition of cell proliferation via G1-phase arrest. [score:5]
Gu et al. reported that miR-490-3p inhibits proliferation of A549 lung cancer cells [24], and Zhang et al. showed that miR-490-3p modulates cell growth and epithelial to mesenchymal transition (EMT) of hepatocellular carcinoma cells [21]. [score:3]
Our RT-PCR results showed that A2780/Taxol has higher miR-490-3P mRNA expression level than A2780. [score:3]
RT-PCR results showed that A2780/Taxol has higher miR-490-3P mRNA expression level than A2780 (A). [score:3]
These studies suggest us that miR-490-3P may also contribute to tumor development; however, its function in the development of drug resistance hasn’t been studied. [score:3]
RT-PCR results showed that A2780/Taxol has higher miR-490-3P mRNA expression level than A2780 (Figure  1A, p < 0.05). [score:3]
Our results showed higher miR-490-3P mRNA expression level in A2780/Taxol cells than in A2780 cells (p < 0.05). [score:3]
Until now, miR-490-3P has been validated to act as a regulator of cell proliferation, migration, invasion, or in the EMT in hepatocellular carcinoma cells and vascular smooth muscle cells [21, 22], but its function in ovarian cancer cell lines has not been reported yet. [score:2]
We demonstrated for the first time that miR-490-3P may be involved in the development of drug resistance in ovarian cancer cells. [score:2]
Following miR-490-3P transfection, both A2780 and A2780/Taxol cells showed decreased sensitivity to paclitaxel. [score:1]
Cell lines were kept in a humidified atmosphere of 5% CO [2] at 37°C with or without paclitaxel treatment and miR-490-3P transfection. [score:1]
Our results showed that the sensitivity of both A2780 and A2780/Taxol cell lines transfected with miR-490-3P mimics to paclitaxel was decreased compared with negative control or mock cells, suggesting that microRNA 490-3P may be involved in the development of drug resistance in ovarian cancer cells. [score:1]
miR-490-3P decreased sensitivity of the A2780 and A2780/Taxol cells to paclitaxel. [score:1]
This is the first study to determine the drug-resistance function of miR-490-3P in the ovarian cancer cell lines. [score:1]
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4
[+] score: 21
In order to further understand the role of aberrant miRNAs in physiological functions and biologic processes in arsenite -induced neoplastic transformation cells, 11 downregulated miRNAs (miR-197-3p, miR-192-5p, miR-127-3p, miR-139-5p, miR-490-3p, miR-196b-5p, miR-125a-3p, miR-298, miR-542-3p, miR-15b-5p, and miR-33b-5p) and six upregulated miRNAs (miR-200b-3p, miR-106b-5p, miR-574-5p, miR-320d, miR-200c-3p, and miR-141-3p) (Table S2) were selected, and their target genes were predicted with the TargetMiner, miRDB, and TarBase databases. [score:11]
Among the 191 dysregulated miRNAs, seventeen miRNAs (downregulation miRNAs: miR-197-3p, miR-192-5p, miR-127-3p, miR-139-5p, miR-490-3p, miR-196b-5p, miR-125a-3p, miR-298, miR-542-3p, miR-15b-5p, miR-33b-5p; upregulation miRNAs: miR-200b-3p, miR-106b-5p, miR-574-5p, miR-320d, miR-200c-3p, miR-141-3p, Table S2) were selected for bioinformatics analysis. [score:8]
On the contrary, miRNAs like miR-197-3p, miR-127-3p, and miR-490-3p regulate few genes and are on the edge of the network, suggesting these miRNAs were less important in arsenite-transformed cells. [score:2]
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5
[+] score: 21
hsa-miR-96 was the most significantly upregulated miRNA and hsa-miR-490-5p was the most significantly downregulated one. [score:7]
The most significantly upregulated miRNA hsa-miR-96 has been reported to be oncogenic [18], but the role of the most significantly downregulated miRNA hsa-miR-490-5p is not clear. [score:7]
hsa-miR-96 (log [2]Ratio = 4.664328) was the most significantly upregulated miRNA and hsa-miR-490-5p (log [2]Ratio = −5.79794) was the most significantly downregulated one (Table 1). [score:7]
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6
[+] score: 20
were down-regulated after miR-490-3P transfection…” should be “…were up-regulated…” In Page 3, Results: the title of the second paragraph was “miR-490-3P down-regulate expression of MDR1/P-gp and GST-π”, should be “miR-490-3P up-regulate…” In Page 4, the title of Figure 2 Legend should be “miR-490-3P up-regulate expression of MDR1/P-gp and GST-π”. [score:20]
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7
[+] score: 18
Interestingly, 14 of these miRNAs (miR-596, miR-630, miR-422a, miR-490-5p, miR-375, miR-708, miR-345, miR-125b-2, miR-516a-3p, miR-135a, miR-1228, miR-1915, miR-134, and miR-663) have established roles in tumor suppression and drug resistance, while 5 miRNAs (miR-630, miR-375, miR-345, miR-1228, and miR-134) are known to inhibit epithelial–mesenchymal transition and invasion in cancer cells. [score:5]
We were able to find a strong correlation of high expression of miR-596, miR-630, miR-490, miR-375, and miR-708 with overall long-term survival in breast cancer or nasopharyngeal carcinoma patients when compared to low expression of the respective miRNA in the same cohorts of patients. [score:4]
Five highly up-regulated miRNA 12 h after 8 mM ascorbate treatment (miR-596, miR-630, miR-490, miR-375, and miR-708) were analyzed using the free online MIRUMIR database (28), which is incorporated into BioProfiling. [score:4]
A significant correlation of high expression of miR-596, miR-630, miR-490, miR-375, and miR-708 with overall long-term survival in breast cancer (GEO dataset IDs: GSE37405 and GSE37405) or nasopharyngeal carcinoma patients (GEO dataset ID: GSE36682) was observed when compared to low expression of the respective miRNA. [score:4]
The Kaplan–Meier plots as depicted in Figure 7 were automatically generated by MIUMIR upon submission of the respective miRNAs (miR-596, miR-630, miR-490, miR-375, and miR-708). [score:1]
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8
[+] score: 14
Here, we intended to identify suitable MREs for bladder cancer specific adenovirus -mediated TRAIL expression from the miRNAs with downregulated expression in bladder cancer, including miR-1 [18- 21], miR-99a [22], miR-100 [23], miR-101 [24, 25], miR-125b [23, 26, 27], miR-133a [18, 20, 21, 23, 28- 30], miR-143 [22, 23, 31- 33], miR-145 [21, 23, 29- 31, 34], miR-195-5p [35], miR-199a-3p [36], miR-200 [37, 38], miR-203 [39, 40], miR-205 [37], miR-218 [21, 41], miR-490-5p [42], miR-493 [43], miR-517a [44], miR-574-3p [45], miR-1826 [46] and let-7c [42]. [score:8]
The involved MREs sequences in our study were described in detail in Table  1. Table 1 MiRNA response elements (MREs) for bladder cancer-specific downregulated miRNAs miRNA primer sequences miR-1Forward: 5′-TCGAGACAAACACC ACATTCCAACAAACACC ACATTCCAACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT TGGAATGTGGTGTTTGT TGGAATGTGGTGTTTGTC-3′ miR-99aForward: 5′-TCGAGACAAACACC TACGGGTACAAACACC TACGGGTACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT ACCCGTAGGTGTTTGT ACCCGTAGGTGTTTGTC-3′ miR-101Forward: 5′-TCGAGACAAACACC GTACTGTACAAACACC GTACTGTACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT ACAGTACGGTGTTTGT ACAGTACGGTGTTTGTC-3′ miR-133Forward: 5′-TCGAGACAAACACC GGACCAAAACAAACACC GGACCAAAACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT TTTGGTCCGGTGTTTGT TTTGGTCCGGTGTTTGTC-3′ miR-218Forward: 5′-TCGAGACAAACACC AAGCACAAACAAACACC AAGCACAAACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT TTGTGCTTGGTGTTTGT TTGTGCTTGGTGTTTGTC-3′ miR-490-5pForward: 5′-TCGAGACAAACACC ATCCATGACAAACACC ATCCATGACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT CATGGATGGTGTTTGT CATGGATGGTGTTTGTC-3′ miR-493Forward: 5′-TCGAGACAAACACC ACCTTCAACAAACACC ACCTTCAACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT TGAAGGTGGTGTTTGT TGAAGGTGGTGTTTGTC-3′ miR-517aForward: 5′-TCGAGACAAACACC TGCACGAACAAACACC TGCACGAACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT TCGTGCAGGTGTTTGT TCGTGCAGGTGTTTGTC-3′The underscored sequences indicated MREs of miR-1, miR-99a, miR-101, miR-133 and miR-218, miR-490-5p, miR-493 and miR-517a. [score:3]
The involved MREs sequences in our study were described in detail in Table  1. Table 1 MiRNA response elements (MREs) for bladder cancer-specific downregulated miRNAs miRNA primer sequences miR-1Forward: 5′-TCGAGACAAACACC ACATTCCAACAAACACC ACATTCCAACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT TGGAATGTGGTGTTTGT TGGAATGTGGTGTTTGTC-3′ miR-99aForward: 5′-TCGAGACAAACACC TACGGGTACAAACACC TACGGGTACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT ACCCGTAGGTGTTTGT ACCCGTAGGTGTTTGTC-3′ miR-101Forward: 5′-TCGAGACAAACACC GTACTGTACAAACACC GTACTGTACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT ACAGTACGGTGTTTGT ACAGTACGGTGTTTGTC-3′ miR-133Forward: 5′-TCGAGACAAACACC GGACCAAAACAAACACC GGACCAAAACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT TTTGGTCCGGTGTTTGT TTTGGTCCGGTGTTTGTC-3′ miR-218Forward: 5′-TCGAGACAAACACC AAGCACAAACAAACACC AAGCACAAACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT TTGTGCTTGGTGTTTGT TTGTGCTTGGTGTTTGTC-3′ miR-490-5pForward: 5′-TCGAGACAAACACC ATCCATGACAAACACC ATCCATGACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT CATGGATGGTGTTTGT CATGGATGGTGTTTGTC-3′ miR-493Forward: 5′-TCGAGACAAACACC ACCTTCAACAAACACC ACCTTCAACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT TGAAGGTGGTGTTTGT TGAAGGTGGTGTTTGTC-3′ miR-517aForward: 5′-TCGAGACAAACACC TGCACGAACAAACACC TGCACGAACAAACACCGC-3′Reverse: 5′-GGCCGCGGTGTTTGT TCGTGCAGGTGTTTGT TCGTGCAGGTGTTTGTC-3′The underscored sequences indicated MREs of miR-1, miR-99a, miR-101, miR-133 and miR-218, miR-490-5p, miR-493 and miR-517a. [score:3]
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9
[+] score: 13
Another recent study shows that overexpressing proline-rich polypeptide (PRP-1) inhibited mTORC1, which resulted in upregulation of certain tumor suppressor miRNAs (miR-125b, miR-192) and downregulation of oncomiRs (miR-550, miR-589, miR-490-3p) (Galoian et al., 2014). [score:13]
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10
[+] score: 10
Based on the collected miRNA-circRNA interactions and the deregulated RNA molecules, we collected several abnormally expressed miRNAs, including 11 downregulated miRNAs (miR-124-3p, miR-129-5p, miR-135a-5p, miR-153-3p, miR-204-5p, miR-208a-3p, miR-211-5p, miR-218-5p, miR-488-3p, miR-490-3p, and miR-504-5p) and 1 upregulated miRNA (miR-373-3p). [score:10]
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11
[+] score: 8
For example, in mammals, miR-7, miR-129-5p, miR-490-3p and miR-204 and miR-211 have been proven to act as tumor suppressors [21], inhibiting the progression and proliferation of hepatocellular carcinoma [22], pulmonary and intestinal carcinoma [23] and breast cancer [24]. [score:5]
Zhang L. Liu M. Li X. Tang H. miR-490-3p Modulates Cell Growth and Epithelial to Mesenchymal Transition of Hepatocellular Carcinoma Cells by Targeting Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 3 (ERGIC3)J. Biol. [score:3]
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12
[+] score: 7
miR-424, miR-542-3p, and miR-454 were the most upregulated, and miR-494, miR-490-5p, and miR-486-5p were the most downregulated miRNAs in tumor in comparison to the normal tissue (28). [score:7]
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13
[+] score: 6
In mice with AFL, miR-199-3p, miR-214, miR-93, miR-146a, miR-191, and let-7b are downregulated and miR-129, miR-490, miR-21, miR-503, miR-183, and miR-185 are upregulated compared with healthy mice [103]. [score:6]
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[+] score: 6
Twelve miRNAs were upexpressed, while 84 miRNAs (has-miR-137, hsa-miR-133a, hsa-miR-143, hsa-miR-363, hsa-miR-4770, hsa-miR-490-5p, hsa-miR-133b, and so on) were deexpressed in tumors compared with those in normal tissues (adjusted P = 0.05) (Figure  1). [score:4]
After a series of selection processes independently with enter method and conditional forward method in conditional logistic regression, we found nine statistically significant miRNAs in enter method, namely, miR574-3p, miR422a, miR490-3p, miR-374b, miR-133a, let7g, miR-378*, miR-9* and miR-378i. [score:1]
Preliminary results showed that the level of miR874-3p, miR-422a, miR-490-3p, miR-374b, miR-133a, let-7 g, miR-378, miR-9*, and miR-378i were all deregulated in the CRC tissues compared with the neighboring noncancerous colorectal tissues (all P < 0.05) (Figure  2). [score:1]
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We also found that miR-20a and miR-490-3p upregulate TNKS2 and ERGIC3, respectively, by targeting their 3′UTRs 13 14. [score:6]
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Our previous studies have identified various miRNAs functioning as tumor suppressors in bladder cancer, including miR-101, miR-124-3p, miR-320c, miR-433, miR-409-3p, miR-490-5p, and miR-576-3p, which regulate the proliferation, migration and invasion of bladder cancer cells by down -regulating various oncogenes [17– 23]. [score:5]
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We then analyzed the GO terms associated to targets of the microRNA families highly expressed in CM, including miR-1 (mir-1 and mir-206), miR-133 (miR-133a and b), miR-208 (miR-208a and b), miR-490, miR-499 and miR-143. [score:5]
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[+] score: 5
Interestingly, according to the miRNA target finding algorithm TargetScan the 3’UTR of leptin harbors putative miRNA binding sites for miR-9, miR-490, miR-29 family, miR-27 family and miR-128 [44]. [score:5]
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[+] score: 4
Other miRNAs from this paper: hsa-mir-143
Moreover, Tian et al. (21), confirmed that miR-490-3p enhanced cisplatin sensitivity of ovarian cancer cells through down -regulating ABCC2 expression. [score:4]
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A study suggested upregulation of SMARCD1 protein through miR-490-3p in H. pylori associated GC. [score:4]
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21
[+] score: 4
MiR-490-5p inhibits proliferation of bladder cancer cells by targeting c-Fos [21]. [score:4]
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22
[+] score: 4
In addition, metformin modulated the expression of a number of miRNAs (let-7f, miR-30b, miR-362, miR-376c, miR-466h, miR-490, and miR-574) involved in the regulation of the cell cycle, which is a crucial mechanism in the AMPK -mediated activity of this drug 42. [score:4]
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miRNAs not different among tissue groups: 7 miRNAs i. e., miR-1231, miR-1, miR-602, miR-4440, miR-133b, miR-23b-5p and miR-490 were expressed in tissues of NEC, SIP and Surg-CTL. [score:3]
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[+] score: 3
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-19a, hsa-mir-20a, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-26a-1, hsa-mir-30a, hsa-mir-33a, hsa-mir-96, hsa-mir-98, hsa-mir-103a-2, hsa-mir-103a-1, mmu-let-7g, mmu-let-7i, mmu-mir-23b, mmu-mir-30a, mmu-mir-30b, mmu-mir-99b, mmu-mir-125a, mmu-mir-125b-2, mmu-mir-9-2, mmu-mir-133a-1, mmu-mir-146a, mmu-mir-155, mmu-mir-182, mmu-mir-183, mmu-mir-24-1, mmu-mir-191, mmu-mir-199a-1, hsa-mir-199a-1, mmu-mir-200b, hsa-mir-30c-2, hsa-mir-30d, mmu-mir-30e, hsa-mir-181b-1, hsa-mir-182, hsa-mir-183, hsa-mir-199a-2, hsa-mir-199b, hsa-mir-221, hsa-mir-223, hsa-mir-200b, mmu-mir-299a, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-191, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-146a, mmu-mir-30c-1, mmu-mir-30c-2, mmu-mir-30d, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-20a, mmu-mir-21a, mmu-mir-23a, mmu-mir-24-2, mmu-mir-26a-1, mmu-mir-96, mmu-mir-98, mmu-mir-103-1, mmu-mir-103-2, mmu-mir-148b, mmu-mir-351, hsa-mir-200c, hsa-mir-155, hsa-mir-181b-2, mmu-mir-19a, mmu-mir-25, mmu-mir-200c, mmu-mir-223, mmu-mir-26a-2, mmu-mir-221, mmu-mir-199a-2, mmu-mir-199b, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-181b-1, mmu-mir-125b-1, hsa-mir-30c-1, hsa-mir-299, hsa-mir-99b, hsa-mir-30e, hsa-mir-26a-2, hsa-mir-361, mmu-mir-361, hsa-mir-365a, mmu-mir-365-1, hsa-mir-365b, hsa-mir-375, mmu-mir-375, hsa-mir-148b, mmu-mir-133a-2, mmu-mir-133b, hsa-mir-133b, mmu-mir-181b-2, mmu-mir-433, hsa-mir-429, mmu-mir-429, mmu-mir-365-2, hsa-mir-433, hsa-mir-193b, hsa-mir-92b, mmu-mir-490, mmu-mir-193b, mmu-mir-92b, hsa-mir-103b-1, hsa-mir-103b-2, mmu-mir-299b, mmu-mir-133c, mmu-let-7j, mmu-mir-30f, mmu-let-7k, mmu-mir-9b-2, mmu-mir-9b-1, mmu-mir-9b-3
The 3′ UTR of mouse Xbp1 mRNA contains several putative target sites for miR-199, miR-299, miR-433, miR-221, and miR-490. [score:3]
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Notwithstanding, the “mut” allele generates binding sites for six alternative miRNAs (Table  1) of which four, miR-143 (3p and 5p), miR-490-3p, miR-1246, and miR-1261, were expressed in the human 1.1E7 pancreatic cell line (Fig.   5a). [score:3]
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[+] score: 3
Taking all four aforementioned sample types together, miR-302b, miR-490-5p, and miR-155 were concomitantly differentially expressed between all. [score:3]
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27
[+] score: 3
Increased expression of miR-494, miR-769-3p and miR-490-3p was associated with ≥70% coronary stenosis. [score:3]
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[+] score: 2
Gga-miR-490-5p, gga-miR-chr13_10137 and gga-miR-chrUn_AADN03024004_45551 were unique to the infected chickens. [score:1]
The highest fold change (71.24) was observed for gga-miR-490- 5p and the lowest fold change (2.06) for gga-miR-193b-3p. [score:1]
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Others with fold changes greater than 6-fold included miR-145*, -137, -133a, -4470, -143, -163, and miR-490-5p. [score:1]
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3/3p21.32 −3.91 hsa-miR-487b 14q32.2 −3.82(29) hsa-miR-487a 14q32.2 −3.78 hsa-miR-758 14q32.2 −3.65 hsa-miR-485-5p 14q32.2 −3.60 hsa-miR-138-1* 16q13.3/3p21.32 −3.55 hsa-miR-382 14q32.2 −3.53(12, 29) hsa-miR-504 Xq26.3 −3.45(52) hsa-miR-128 2q21.3/3p22.3 −3.43(12, 14, 51, 59) hsa-miR-490-5p 7q33 −3.42 hsa-miR-770-5p 14q32.2 −3.35 hsa-miR-410 14q32.2 −3.30(29) hsa-miR-432 14q32.2 −3.29 hsa-miR-485-3p 14q32.2 −3.02 hsa-miR-490-3p 7q33 −2.88 hsa-miR-381 14q32.2 −2. [score:1]
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Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-16-1, hsa-mir-21, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-26b, hsa-mir-29a, hsa-mir-30a, hsa-mir-31, hsa-mir-32, hsa-mir-33a, hsa-mir-96, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-16-2, hsa-mir-192, hsa-mir-199a-1, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-10a, hsa-mir-10b, hsa-mir-34a, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-182, hsa-mir-183, hsa-mir-199a-2, hsa-mir-199b, hsa-mir-203a, hsa-mir-204, hsa-mir-211, hsa-mir-212, hsa-mir-181a-1, hsa-mir-214, hsa-mir-217, hsa-let-7g, hsa-let-7i, hsa-mir-15b, hsa-mir-27b, hsa-mir-122, hsa-mir-125b-1, hsa-mir-132, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-137, hsa-mir-138-2, hsa-mir-145, hsa-mir-152, hsa-mir-153-1, hsa-mir-153-2, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, hsa-mir-126, hsa-mir-127, hsa-mir-136, hsa-mir-138-1, hsa-mir-146a, hsa-mir-150, hsa-mir-185, hsa-mir-193a, hsa-mir-194-1, hsa-mir-320a, hsa-mir-155, hsa-mir-181b-2, hsa-mir-194-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-34c, hsa-mir-26a-2, hsa-mir-302b, hsa-mir-369, hsa-mir-375, hsa-mir-378a, hsa-mir-328, hsa-mir-335, hsa-mir-133b, hsa-mir-409, hsa-mir-484, hsa-mir-485, hsa-mir-486-1, hsa-mir-495, hsa-mir-193b, hsa-mir-497, hsa-mir-512-1, hsa-mir-512-2, hsa-mir-506, hsa-mir-509-1, hsa-mir-532, hsa-mir-92b, hsa-mir-548a-1, hsa-mir-548b, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-548c, hsa-mir-33b, hsa-mir-548d-1, hsa-mir-548d-2, hsa-mir-1224, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-320b-2, hsa-mir-378d-2, hsa-mir-802, hsa-mir-509-2, hsa-mir-509-3, hsa-mir-548e, hsa-mir-548j, hsa-mir-548k, hsa-mir-548l, hsa-mir-548f-1, hsa-mir-548f-2, hsa-mir-548f-3, hsa-mir-548f-4, hsa-mir-548f-5, hsa-mir-548g, hsa-mir-548n, hsa-mir-548m, hsa-mir-548o, hsa-mir-548h-1, hsa-mir-548h-2, hsa-mir-548h-3, hsa-mir-548h-4, hsa-mir-548p, hsa-mir-548i-1, hsa-mir-548i-2, hsa-mir-548i-3, hsa-mir-548i-4, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, hsa-mir-548q, hsa-mir-548s, hsa-mir-378b, hsa-mir-548t, hsa-mir-548u, hsa-mir-548v, hsa-mir-548w, hsa-mir-320e, hsa-mir-548x, hsa-mir-378c, hsa-mir-4262, hsa-mir-548y, hsa-mir-548z, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548o-2, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-548h-5, hsa-mir-548ab, hsa-mir-378f, hsa-mir-378g, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-378h, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548x-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-378i, hsa-mir-548am, hsa-mir-548an, hsa-mir-203b, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-378j, hsa-mir-548ay, hsa-mir-548az, hsa-mir-486-2, hsa-mir-548ba, hsa-mir-548bb, hsa-mir-548bc
Among these only four of them (miR-185, miR199a-3p, miR-214 and miR-490) were shown to be similarly altered in liver tissue and circulation [29]. [score:1]
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Zhang W. Zhang C. Chen H. Li L. Tu Y. Liu C. Shi S. Zen K. Liu Z. Evaluation of microRNAs miR-196a, miR-30a-5P, and miR-490 as biomarkers of disease activity among patients with FSGS Clin. [score:1]
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They reported CRC-specific previous associations between miR-101 and miR-320 and β-catenin, miR-224 and GSK3β, and miR-490-3p and FRAT1. [score:1]
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Zhang W. Zhang C. Chen H. Li L. Tu Y. Liu C. Shi S. Zen K. Liu Z. Evaluation of microRNAs miR-196a, miR-30a-5p, and miR-490 as biomarkers of disease activity among patients with FSGSClin. [score:1]
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