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20 publications mentioning hsa-mir-517b

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-517b. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 50
According to TargetScan predictions [45], this shift results in base-pairing between TNIP1 and nucleotides 2 to 7 of miR-517b as opposed to nucleotides 2 to 8 in miR-517a/c-the latter resulting in a more favorable miRNA-target interaction. [score:5]
Also, miR-517b did not phenocopy miR-517a/c in secondary screens (Figure 3A) or knockdown TNIP1 protein expression (Figure 5A). [score:4]
Effect of miR-517 family on endogenous NF-κB targets and signaling components. [score:3]
In unstimulated cells, miR-517a/c strongly induced IL8 (>160-fold) and TNF (>40-fold) expression while miR-517b had only a modest effect (13- and 4-fold, respectively), confirming the reporter results (Figure 3A). [score:3]
We decided to follow up on the miR-517 family with additional experiments for several reasons: 1) miRNAs typically have a moderate impact on their targets yet miR-517a/c were potent activators of reporter activity in the absence of any added stimulus; 2) we were intrigued that miR-517b did not activate signaling despite extensive sequence similarity to miR-517a/c; and 3) a previous proteomics study by Luo et al. suggested miR-517a involvement in MAPK and TNF signaling in BeWo cells [23]. [score:3]
Next, we wanted to test the effect of the miR-517 miRNAs on endogenous NF-κB targets. [score:3]
miR-517a/c, but not miR-517b, increased nuclear p65 and decreased cytoplasmic IκB-α expression relative to the negative control (Figure 4D). [score:3]
miR-517b also inhibited reporter activity, but to a lesser extent (Figure 5F). [score:3]
However, in cells treated with TNF, there was no significant change in IL8 and TNF expression in cells transfected with the miR-517 mimics relative to the negative control (Figure 4C). [score:3]
Hence, the shift in the miR-517b seed likely alters target interaction relative to miR-517a/c. [score:3]
Overexpression of miR-517b caused a relatively small increase in reporter activity (2.6-fold) and hence, was deemed a true -negative. [score:3]
miR-517a and miR-517c differ by one nucleotide at position 12, and miR-517b is shifted by one nucleotide relative to miR-517a (Figure 4A). [score:1]
These differences were likely due to a single nucleotide shift in the miR-517b mature sequence relative to miR-517a/c (Figure 4A). [score:1]
We informed the curators of miRBase, and the unshifted sequence (identical to miR-517a) is the new reference for miR-517b (now miR-517b-3p) as of miRBase v17. [score:1]
Interestingly, miR-517b was not a hit in our screen despite extensive homology to miR-517a/c (Figure 4A). [score:1]
The miR-517 family consists of three highly homologous members, miR-517a, miR-517b, and miR-517c, yet intriguingly, miR-517b, was not a hit in the primary screen. [score:1]
Both miR-517a and miR-517c, but not miR-517b, potently reduced TNIP1 protein levels (Figure 5A). [score:1]
The repression by the miR-517 miRNAs was significantly alleviated in the minor allele reporter (Figure 5F). [score:1]
In all, these results confirmed the primary and secondary screen data that miR-517a/c, and not miR-517b, were potent activators of NF-κB signaling. [score:1]
We transfected HEK293 cells with the miR-517 mimics and measured the expression of IL8 and TNF mRNA in unstimulated cells (Figure 4B) and in cells stimulated with TNF for three hours (Figure 4C). [score:1]
The miR-517 family is located in a large, primate-specific miRNA cluster on human chromosome 19 (C19MC) [24]. [score:1]
Nonetheless, we included miR-517b in the secondary screen to eliminate the possibility that it was a false -negative. [score:1]
Interestingly, during the preparation of this study we examined sequence reads of the miR-517b locus from miRBase [46], the central repository for miRNA sequences, and found a variant identical to miR-517a. [score:1]
The miR-517 miRNAs share extensive sequence homology. [score:1]
This shift in miR-517b also results in a shifted seed sequence (Figure 4A). [score:1]
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2
[+] score: 17
Recently it has been shown that mir-517b can be transported by trophoblast exosomes to recipient cells, normally not expressing C19MC miRNAs, and inhibit viral infections [54]. [score:5]
Mir-517a, mir-517b and mir-518b were chosen because of their placenta specificity [36] and mir-518b being dys-regulated in PE [41]. [score:2]
Three miRNAs were down regulated in the 10K STBMs after Hb treatment; mir-517a, mir-141 and mir-517b. [score:2]
After Hb perfusion, mir-517a (p = 0.03671), mir-141 (p = 0.01219) and mir-517b (p = 0.03671) were significantly down regulated in 10K STBMs (Figure 3). [score:2]
Mir-517a, mir-141 and mir-517b were down regulated after Hb perfusion in the 10K STBMs. [score:2]
Mir-517a and mir-517b belong to the C19MC cluster [32]. [score:1]
Of particular interest is the alteration of two placenta specific micro -RNAs; mir-517a and mir-517b. [score:1]
All miRNAs (mir-517a, mir-517b, mir-518b, mir-205, mir-210, mir-222, mir-141, mir-16 and mir-424) analysed in this study were present in both 10K and 150K STBMs. [score:1]
org), which differs two nucleotides from the human hsa-mir-517b-3p. [score:1]
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3
[+] score: 13
Eisenberg et al. also showed that miR-517* was up-regulated only in adult FSHD1 biopsies and not in any of the 9 other neuromuscular diseases studied by these authors, suggesting that this microRNA might be a potent biomarker for FSHD [51]. [score:6]
However, we identified 8 miRNAs exclusively expressed in FSHD1 samples (miR-330, miR-331-5p, miR-34a, miR-380-3p, miR-516b, miR-582-5p, miR-517* and miR-625) which could represent new biomarkers for this disease. [score:5]
We did not observed any miRNAs only detected in control samples but we identified 8 miRNAs which were only expressed in FSHD samples as compared to age-matched controls: miR-330, miR-331–5p, miR-34a, miR-380–3p, miR-516b, miR-582–5p, miR-517* and miR-625. [score:2]
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4
[+] score: 10
The enriched GO categories for the downregulated targets identified for some of the individual upregulated miRNAs were as follows: Cell Homeostasis and Peroxisome for miR-34a; Cell Cycle and DNA Damage for miR-181b; and Vesicle Processes for miR-517*. [score:9]
Represented GO categories were also associated with miRNAs, such as: Cell Cycle with miR-200a and DNA Damage with miR-517*. [score:1]
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5
[+] score: 10
We performed Monte Carlo analysis on the 2102Ep and NTera-2 differential gene expression datasets and cross-referencing with the results with the differential miRNA expression results revealed 10 miRNAs in 2102Ep cells (mir-26a, miR-28, miR-30c, miR-148a, miR-200b, miR-517b, miR-518a-3p, miR-518b, miR-518c, miR-518f) and two miRNAs in NTera-2 cells (miR-200c and miR-367) to be potential master regulators of their inversely regulated target genes. [score:9]
While miR-26a, miR-30c, miR-148a, miR-200b, miR-200c and miR-367 are broadly conserved across vertebrate species, miR-28 is conserved only in mammals and miR-517b, miR-518f, miR-518b, miR-518c, miR-518a-3p, all as members of the C19MC polycistron, are found only in primates. [score:1]
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6
[+] score: 9
MicroRNAs similarly expressed in both AD-affected brain and PBMC are identified (Table 2; Fig. 1), including the up-regulation of miR-34a, miR-200a and miR-520h, as well as the up-regulation let-7f, miR-371 and miR-517/517* observed in the CSF and PBMC of AD patients. [score:9]
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7
[+] score: 7
One miRNA, miR-517* was reported to be uniquely up-regulated in FSHD. [score:4]
While miR-517* was examined in our study, the expression was not detectable in either the FSHD and control myoblasts. [score:3]
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8
[+] score: 7
For example, mir-202, mir-17-3p, mir-517 targets are highly expressed in EMT tumors and lowly expressed in the more luminal tumors. [score:7]
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9
[+] score: 6
These miRNAs were differentially-expressed upon NaB -induced differentiation and represent ES miRNAs (hsa-miR-302a*, hsa-miR-302d, hsa-miR-517b), endodermal miRNAs (hsa-miR-122, hsa-miR-375) and miRNAs that were upregulated in both lines (hsa-miR-10a, hsa-miR-24). [score:6]
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10
[+] score: 6
Other miRNAs from this paper: hsa-mir-451a, hsa-mir-517a, hsa-mir-451b
For miR-517a and miR-517b, despite the fact that we observed their higher expression in placenta samples, both presented very low or undetectable expression in all the human normal tissues evaluated (data not shown). [score:3]
Among the top-five most highly expressed miRNAs in normal human placenta, we evaluate the expression of miR-451, miR-517a, miR-517b and miR-720 in normal human placenta samples, several normal human tissues and different cancer cell lines using RT-qPCR. [score:3]
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11
[+] score: 6
Similarly, Hromadnikova et al. [17] detected a downregulation of 6 miRNAs (miR-517-5p, miR-518f-5p, miR-519a, miR-519d, miR-520a-5p, and miR-525) in placental tissues of 36 FGR pregnancies: compared to the previous studies, these results seem more robust since that authors investigated more types of miRNAs and used those that were previously demonstrated to be exclusively expressed or highly expressed in placental tissues. [score:5]
A significant elevation of several extracellular placenta-specific miRNA levels was recently showed (miR-516-5p, miR-517, miR-518b, miR-520a, miR-520h, miR-525, and miR-526a,) during early gestation in 7 pregnancies with later onset of preeclampsia and/or FGR [44]. [score:1]
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12
[+] score: 4
Our data in the present study are consistent with the above findings showing the upregulation of miR-517, miR-92a, miR-127, and miR-181a in Exo [Hypoxic]. [score:4]
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13
[+] score: 4
Hromadnikova et al. reported an upregulation of circulating C19MC miRNAs (miR-516-5p, miR-517*, miR-520a*, miR-525, and miR526a) in patients with PE (33). [score:4]
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14
[+] score: 3
ISH has also been used to detect the expression of miR-517b in STBs and villous stroma cells [82]. [score:3]
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15
[+] score: 3
In comparison, soft tissue tumors and non-neoplastic muscle tissues showed uniformly higher level expression of miR-517a and miR-517b (highlighted in red in Figure 1). [score:3]
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16
[+] score: 2
This analysis included miR-184, miR-548a-5p, miR-574-3p, miR-616, miR-1233, miR-642, miR-140-3p, miR-517b, miR-10b and miR-380-3p (Figure  3, Tables  3 and 4 and Additional file 3: Table S3). [score:1]
Also, signal intensities for miR-548a-5p, miR-517b and miR-380-3p were very low and not detected in most samples. [score:1]
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17
[+] score: 1
45 (10) COL1A1, COL4A1, COL4A2, COL4A5, COL5A20.013HSA-MIR-98.45 (10) COL1A1, COL4A1, COL4A2, COL4A5, COL5A20.0111HSA-MIR-657.45 (13) FKTN, UST, GALNT10, XYLT1, GLT8D20.0113HSA-MIR-517B. [score:1]
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18
[+] score: 1
07] 0.01 1p36.33 hsa-miR-126 −0.36 [−0.64 – −0.08] 0.01 9q34.3 hsa-miR-888 −0.56 [−1.02 – −0.10] 0.02 Xq27.3 hsa-miR-517b −0.22 [−0.41 – −0.03] 0.03 19q13.42 hsa-miR-363 −0.43 [−0.80 – −0.05] 0.03 Xq26.2 hsa-miR-216b −0.26 [−0.50 – −0.03] 0.03 2p16.1 hsa-miR-1285 −0.27 [−0.53 – −0.02] 0.04 7q21. [score:1]
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19
[+] score: 1
Besides these 9 miRNAs, we also identified 12 more miRNAs in this cluster; they were miR-515-5p, miR-517a, miR-517b, miR-517c, miR-519e, miR-520b, miR-520d, miR-520f, miR-520h, miR-521, miR-525-3p, and miR-526b*. [score:1]
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20
[+] score: 1
MNPT) miR-449a# 3.92 miR-32 3.49 miR-548c-5p 2.71 miR-562 2.56 miR-103-as 2.53 miR-512-3p 2.41 miR-200c* 2.33 miR-147b 2.24 miR-770-5p 2.09 miR-518c* 2.00 miR-517b 1.88 miR-182 1.79 miR-615-3p 1.70 miR-496 1.59 miR-1200 1.58 miR-375 1.54 miR-551a 1.53 *Passanger strand. [score:1]
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