1 |
[+]
score:
42
Other miRNAs from this paper: hsa-mir-19b-1, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-29c, hsa-mir-30e, hsa-mir-373, hsa-mir-381, hsa-mir-526b, hsa-mir-654, bta-mir-103-1, bta-mir-30e, bta-mir-29c, bta-mir-103-2, hsa-mir-103b-1, hsa-mir-103b-2, bta-mir-381, bta-mir-654
Based on the criteria mentioned in material and methods part we found ITGA3 (as target of miR-654-5p), SOCS4 & MAP3K1 (as target of miR-640), BRMS1L & ZNFX1 (as target of miR-526b*) and CD44 & VEGFA (as target of miR-373).
[score:9]
0078505.g004 Figure 4Expression patterns of miR-654-5p and miR-640 (up-regulated in exosomal fraction follicular fluid derived from follicles containing BCB- oocytes) and miR-526b* and miR-373 (up-regulated in exosomal fraction of follicular fluid from BCB+ groups) were investigated in surrounding follicular cells namely: cumulus oocyte complex (COCs), granulosa cells (GC), and theca cells (TC) from the same category of follicle which were used for miRNA PCR array analysis.
[score:7]
Expression patterns of miR-654-5p and miR-640 (up-regulated in exosomal fraction follicular fluid derived from follicles containing BCB- oocytes) and miR-526b* and miR-373 (up-regulated in exosomal fraction of follicular fluid from BCB+ groups) were investigated in surrounding follicular cells namely: cumulus oocyte complex (COCs), granulosa cells (GC), and theca cells (TC) from the same category of follicle which were used for miRNA PCR array analysis.
[score:7]
Among the up-regulated miRNAs, miR-654-5p & miR-640, and down-regulated miRNAs, miR-373 & miR-526b*, displayed the greatest fold change difference in the exosomal fraction of follicular fluid.
[score:7]
We choose miR-654-5p, miR-640, miR-526b* and miR-373 because these miRNAs were differentially expressed in exosomal fraction of follicular fluid and they were also substantially expressed in exosomes.
[score:5]
MicroRNAs like miR-640, miR-526b* and miR-381 were abundant at higher level in theca cells, while miR-373, miR-30e* and miR-19b-1* expressed more in COCs.
[score:3]
Importantly, we found that miR-654-5p, miR-640 and miR-526b*, and miR-373 were more abundant in exosomes from follicular fluid of follicles containing BCB- and BCB+ oocytes, respectively (Figure 7 ).
[score:1]
Then the expression level miR-654-5p and miR-640 (enriched in exosomes derived from BCB- follicular fluid) and miR-526b* and miR-373 (enriched in follicular fluid derived from follicles with BCB+ oocyte) were investigated by real time PCR.
[score:1]
While exosomal miRNAs namely, miR-640 and miR-526b* were more abundant in theca cells, miR-373 was detected at higher level in COCs.
[score:1]
The PCR array results revealed that, in follicular fluid from follicles containing growing oocytes (BCB-), miR-654-5p and miR-640 (in exosomal) and miR-19b-1* and miR-29c (in non exosomal) were highly abundant (Table 2 ).
[score:1]
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2 |
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score:
11
Other miRNAs from this paper: hsa-let-7b, hsa-mir-18a, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-26b, hsa-mir-27a, hsa-mir-29a, hsa-mir-30a, hsa-mir-31, hsa-mir-93, hsa-mir-100, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-196a-1, hsa-mir-34a, hsa-mir-182, hsa-mir-196a-2, hsa-mir-221, hsa-mir-222, hsa-mir-200b, hsa-mir-125b-1, hsa-mir-130a, hsa-mir-141, hsa-mir-191, hsa-mir-125b-2, hsa-mir-193a, hsa-mir-200c, hsa-mir-155, hsa-mir-29c, hsa-mir-200a, hsa-mir-26a-2, hsa-mir-378a, hsa-mir-382, hsa-mir-383, hsa-mir-148b, hsa-mir-202, hsa-mir-492, hsa-mir-501, hsa-mir-532, hsa-mir-92b, hsa-mir-575, hsa-mir-617, hsa-mir-622, hsa-mir-626, hsa-mir-378d-2, hsa-mir-891b, hsa-mir-190b, hsa-mir-760, hsa-mir-378b, hsa-mir-378c, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-378j
The top most highly expressed miRNAs in ERBB2 overexpressing cell lines included hsa-let-7b, hsa-miR-640, hsa-miR-200c, hsa-miR-378, hsa-miR-141, hsa-miR-196a, hsa-miR-29c, and hsa-miR-18a*, whereas hsa-miR-501-5p, hsa-miR-202, hsa-miR-760, and hsa-miR-626 were more highly expressed in luminal cell lines lacking ERBB2 overexpression (fold change ≥ 1.5) (see Table S8 in Additional file 1).
[score:9]
Another group of 17 miRNAs (hsa-miR-575, hsa-miR-155, hsa-miR-26b, hsa-miR-200a, hsa-miR-200b, hsa-miR-141, hsa-miR-200c, hsa-miR-190b, hsa-miR-492, hsa-miR-640, hsa-miR-196a, hsa-miR-29c, hsa-miR-93, hsa-miR-193a-3p, hsa-miR-191, hsa-miR-26a, hsa-miR-182) showed significantly higher expression in the major cluster compared with the other miRNAs (fold change ≥ 1.5) (Figure 2, bottom red box).
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10
In response to H [2]S, expression of miR-640 was reduced in HUVECs and HMEC-1 cells through the VEGFR2-mTOR pathway resulting in increased HIF1A expression.
[score:5]
As miR-640 is identified as a key regulator of the proangiogenic effects of H [2]S 22 also locally (intraplaque) increased expression of miR-640 (or miRs with similar effects) might be considered to reduce H [2]S -mediated angiogenesis.
[score:3]
Although not determined by us in HMEC-1 cells, we propose that also in these cells H [2]S might promote angiogenesis involving pathways such as PI3K/AKT and MAPK pathways, as well as ATP-sensitive K-channels 6. Recently, microRNA miR-640 was identified as a key regulator of the proangiogenic effects of H [2]S 22.
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score:
5
Among these miRNAs, miR-640 is down-regulated and regulates the stability of hypoxia-inducible factor 1 alpha (HIF1A) mRNA 14.
[score:5]
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5 |
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score:
4
Other miRNAs from this paper: hsa-mir-21, hsa-mir-181b-1, hsa-mir-222, hsa-mir-223, hsa-mir-130a, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-144, hsa-mir-155, hsa-mir-181b-2, hsa-mir-302a, hsa-mir-296, hsa-mir-130b, hsa-mir-365a, hsa-mir-365b, hsa-mir-342, hsa-mir-424, hsa-mir-451a, hsa-mir-486-1, hsa-mir-493, hsa-mir-520d, hsa-mir-500a, hsa-mir-503, hsa-mir-548b, hsa-mir-550a-1, hsa-mir-550a-2, hsa-mir-661, hsa-mir-550a-3, hsa-mir-892b, hsa-mir-500b, hsa-mir-550b-1, hsa-mir-550b-2, hsa-mir-451b, hsa-mir-486-2
Hsa-miR-548b-3p and hsa-miR-640 showed the minimum degree of regulation, with only 3 and 4 target genes included in the network.
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score:
3
Other miRNAs from this paper: mmu-mir-195a, mmu-mir-302a, hsa-mir-195, hsa-mir-302a, hsa-mir-302b, hsa-mir-302c, hsa-mir-302d, rno-mir-195, hsa-mir-455, mmu-mir-302b, mmu-mir-302c, mmu-mir-302d, mmu-mir-455, rno-mir-455, hsa-mir-302e, hsa-mir-302f, hsa-mir-1909, mmu-mir-195b
We found that our transcript sequence could be a target of three miRNAs: hsa-miR-455-5p, has-miR-640 and has-miR-1909-3p.
[score:3]
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7 |
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score:
3
Other miRNAs from this paper: hsa-mir-20a, hsa-mir-93, mmu-mir-1a-1, mmu-mir-27b, mmu-mir-10b, hsa-mir-198, hsa-mir-10b, hsa-mir-1-2, hsa-mir-27b, mmu-mir-20a, mmu-mir-93, hsa-mir-1-1, mmu-mir-1a-2, hsa-mir-381, mmu-mir-381, hsa-mir-513a-1, hsa-mir-513a-2, hsa-mir-576, hsa-mir-580, hsa-mir-548a-1, hsa-mir-548a-2, hsa-mir-548a-3, hsa-mir-600, hsa-mir-628, hsa-mir-639, hsa-mir-641, hsa-mir-647, hsa-mir-662, hsa-mir-1224, mmu-mir-1224, hsa-mir-888, hsa-mir-887, mmu-mir-1b, hsa-mir-1206, hsa-mir-1304, hsa-mir-548f-1, hsa-mir-548f-2, hsa-mir-548f-3, hsa-mir-548f-4, hsa-mir-548f-5, hsa-mir-1260a, hsa-mir-1261, hsa-mir-1268a, hsa-mir-548h-1, hsa-mir-548h-2, hsa-mir-548h-3, hsa-mir-548h-4, hsa-mir-513b, hsa-mir-1908, hsa-mir-1260b, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-1268b, hsa-mir-548h-5, hsa-mir-548ab, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-548am, hsa-mir-548an, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, hsa-mir-548ay, hsa-mir-548az
Similarly, of 46 suppressed miRNAs, we recognized 14 human-specific non-homologous miRNAs, including miR-1206, miR-548a-5p, miR-548f, miR-576-5p, miR-600, miR-639, miR-640, miR-641, miR-647, miR-662, miR-886-3p, miR-887, miR-628-3p, and miR-888.
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score:
1
Other miRNAs from this paper: hsa-mir-17, hsa-mir-19a, hsa-mir-29a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-198, hsa-mir-208a, hsa-mir-10a, hsa-mir-223, hsa-mir-122, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-125b-1, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, hsa-mir-126, hsa-mir-146a, hsa-mir-150, hsa-mir-155, hsa-mir-29c, hsa-mir-99b, hsa-mir-296, hsa-mir-196b, hsa-mir-515-1, hsa-mir-515-2, hsa-mir-548a-1, hsa-mir-548b, hsa-mir-548a-2, hsa-mir-550a-1, hsa-mir-550a-2, hsa-mir-548a-3, hsa-mir-548c, hsa-mir-548d-1, hsa-mir-548d-2, hsa-mir-550a-3, bta-mir-29a, bta-mir-125b-1, bta-mir-126, bta-mir-10a, bta-mir-124a-1, bta-mir-17, bta-mir-29b-2, bta-mir-29c, bta-mir-150, bta-mir-122, bta-mir-125b-2, bta-mir-19a, bta-mir-99b, hsa-mir-208b, hsa-mir-548e, hsa-mir-548j, hsa-mir-548k, hsa-mir-548l, hsa-mir-548f-1, hsa-mir-548f-2, hsa-mir-548f-3, hsa-mir-548f-4, hsa-mir-548f-5, hsa-mir-548g, hsa-mir-548n, hsa-mir-548m, hsa-mir-548o, hsa-mir-548h-1, hsa-mir-548h-2, hsa-mir-548h-3, hsa-mir-548h-4, hsa-mir-548p, hsa-mir-548i-1, hsa-mir-548i-2, hsa-mir-548i-3, hsa-mir-548i-4, bta-mir-124a-2, bta-mir-124b, bta-mir-146a, bta-mir-155, bta-mir-196b, bta-mir-208a, bta-mir-208b, bta-mir-223, bta-mir-296, bta-mir-29d, bta-mir-9-1, bta-mir-9-2, bta-mir-29e, bta-mir-29b-1, hsa-mir-548q, bta-mir-2284i, bta-mir-2285a, bta-mir-2284s, bta-mir-2285d, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2285b-1, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2284p, bta-mir-2284u, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2285c, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-2284e, hsa-mir-548s, hsa-mir-548t, hsa-mir-548u, hsa-mir-548v, hsa-mir-548w, hsa-mir-548x, bta-mir-2284w, bta-mir-2284x, hsa-mir-548y, hsa-mir-550b-1, hsa-mir-550b-2, hsa-mir-548z, hsa-mir-548aa-1, hsa-mir-548aa-2, hsa-mir-548o-2, hsa-mir-548h-5, hsa-mir-548ab, hsa-mir-548ac, hsa-mir-548ad, hsa-mir-548ae-1, hsa-mir-548ae-2, hsa-mir-548ag-1, hsa-mir-548ag-2, hsa-mir-548ah, hsa-mir-548ai, hsa-mir-548aj-1, hsa-mir-548aj-2, hsa-mir-548x-2, hsa-mir-548ak, hsa-mir-548al, hsa-mir-548am, hsa-mir-548an, hsa-mir-548ao, hsa-mir-548ap, hsa-mir-548aq, hsa-mir-548ar, hsa-mir-548as, hsa-mir-548at, hsa-mir-548au, hsa-mir-548av, hsa-mir-548aw, hsa-mir-548ax, bta-mir-2284y-1, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, hsa-mir-548ay, hsa-mir-548az, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2284y-2, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2284y-3, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-2284y-7, bta-mir-2285n-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2285k-2, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2284z-4, bta-mir-2285k-5, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2284z-2, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2284ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2284ac, bta-mir-2285ae, hsa-mir-548ba, hsa-mir-548bb, hsa-mir-548bc, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
These include the majority of miRNAs numbered from miR-550 to miR-640; some 200 miRNAs, which include the hsa-miR-515 cluster (11 miRNAs), and interestingly, the miR-548 family.
[score:1]
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