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6 publications mentioning mmu-mir-760

Open access articles that are associated with the species Mus musculus and mention the gene name mir-760. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 492
For example, it is reported that miR-760 was strongly overexpressed in ovarian cancer and miR-760 upregulation drastically promoted ovarian cancer cell proliferation by inhibiting the expression of PHLPP2 [37]. [score:10]
Herein, we found that miR-760 was downregulated in CRC tissues and that miR-760 overexpression suppressed CRC cell proliferation by repressing BATF3 expression. [score:10]
Upregulation of miR-760 suppressed CRC cell proliferation, whereas downregulation of miR-760 promoted CRC proliferation in vitro. [score:9]
Furthermore, the results showed that cyclin D1 mRNA was significantly downregulated by ectopic miR-760, whereas it was upregulated by inhibition of miR-760 (Fig. 4f). [score:9]
To confirm the direct regulation of miR-760 on BATF3 expression, we detected BATF3 expression in SW620-760 and HCT116 cells transfected with miR-760 inhibitor. [score:9]
Our present study suggests that miR-760 suppresses cell proliferation and tumorigenicity in CRC cells by targeting BATF3 mRNA and suppressing the expression of BATF3 and downstream cyclin D1. [score:9]
Additionally, we identified BATF3 as a direct target of miR-760, and that the essential biological function of miR-760 during CRC progression both in vitro and in vivo is to suppress the expression of BATF3 and downstream cyclinD1 via AP-1 transcription factor. [score:8]
Moreover, upregulation of miR-760 suppresses proliferation of colorectal cancer cells by targeting BATF3 3′-UTR. [score:8]
It has been shown that miR-760 was downregulated in chemoresistant breast cancer tissues and might mediate breast cancer chemoresistance via the regulation of p-gp expression [35]. [score:7]
The results suggest that silencing BATF3 expression in SW620-psico and miR-760 inhibitor -transfected HCT116 cells can reverse the effect of the miR-760 inhibitor on HCT116 cell proliferation. [score:7]
These results confirmed that miR-760 inhibits CRC cell proliferation by suppressing endogenous BATF3 expression and BATF3 plays an important role in miR-760 -mediated repression of CRC cell proliferation. [score:7]
a Real-time PCR analysis of miR-760 expression in HCT116 cells transfected with miR-760 inhibitor (116-760-in) or miRNA inhibitor negative control (116-NC). [score:7]
The results from both qRT-PCR (Fig. 4d) and western blot (Fig. 4e) revealed that the BATF3 expression level was reduced in miR-760 -overexpressing cells, and its level was restored upon treatment with miR-760 inhibitor. [score:7]
miR-760 substantially suppressed CRC cell proliferation by inhibiting the expression of BATF3 and cyclin D1. [score:7]
We used the publicly available algorithm TargetScan (v7.1)—a public database of microRNAs and their targets—to predict the target(s) of miR-760 in humans. [score:7]
Collectively, these results reveal that miR-760 directly targets BATF3 and inhibits cyclinD1 via AP-1 in CRC cells. [score:6]
It has been previously shown that the JNK -mediated phosphorylation of AP-1 (c-Jun) upregulates cyclin D1 to drive proliferation in liver cell regeneration and mouse epidermal cell transformation [42, 43], which supported our data that miR-760 inhibited cyclinD1 via AP-1 in CRC cells. [score:6]
Fig. 2Upregulation of miR-760 inhibited colorectal cancer cell proliferation. [score:6]
Therefore, cyclin D1 may be tightly regulated by miR-760 in CRC, and miR-760 restoration could target BATF3/AP-1/cyclin D1 pathway to suppress CRC progression. [score:6]
Patients with higher miR-760 expression had significantly lower BATF3 expression compared to those with lower miR-760 expression (P < 0.001, Fig. 7b). [score:6]
miR-760 was markedly downregulated in CRC tissues, and low miR-760 expression was associated with poor prognosis among CRC patients. [score:6]
Moreover, our data indicated that the full-length 3′-untranslated region (3′-UTR) of human BATF3 mRNA was a direct target of miR-760. [score:6]
b Patients with high miR-760 expression had significantly lower BATF3 expression compared with those with low miR-760 expression (P < 0.001). [score:6]
Consistent with the proposed regulation of BATF3 and CRC cell proliferation by miR-760, expression patterns of BATF3 and Ki67 in SW620-miR-760 tumors were similar to the in vitro results, supporting the tumor suppressor function of miR-760 in CRC tumorigenesis (Fig. 6d, 6d). [score:6]
These results suggest that upregulation of miR-760 suppresses the proliferation of CRC cells. [score:6]
Taken together, BATF3 is upregulated in human CRC and miR-760 is reversely related with BATF3 expression in CRC tissues. [score:6]
Bold italics indicate statistically significant values (P < 0.05) As miR-760 had a consistent pattern of downregulation in human CRC samples, we examined the expression pattern of miR-760 in CRC cell lines. [score:6]
To test if BATF3 expression is regulated by miR-760, we cloned the BATF3 3′-UTR into a luciferase reporter plasmid pmir-GLO and quantified expression of the adjacent hRluc coding region. [score:6]
miR-760 inhibited CRC growth by downregulating BATF3/AP-1/ cyclinD1 signaling. [score:6]
Vectors, lentiviral infection, and transfection of miR-760 mimic, inhibitor, and siRNA of target gene. [score:5]
Hsa-miR-760 mimic, the negative control (NC) mimic, hsa-miR-760 inhibitor, and the NC inhibitor were purchased from Genepharma Company (Shanghai, China). [score:5]
The negative regulation of BATF3 by miR-760 leads to downregulation of cyclinD1 in CRC cells. [score:5]
We further found that ectopic expression of miR-760 suppresses proliferation and tumorigenicity in CRC cells. [score:5]
These results suggest that miR-760 binds directly to the predicted binding site(s) in the BATF3 3′-UTR and negatively regulates BATF3 expression. [score:5]
Fig. 6Ectopic expression of miR-760 suppressed tumor growth in vivo. [score:5]
To further investigate the effect of BATF3 reduction on CRC progression, we suppressed endogenous BATF3 expression using a BATF3-specific siRNA in both SW620-psico (Fig.   5a,b) and miR-760 inhibitor -transfected HCT116 cells (Fig. 5d,e). [score:5]
b Real-time PCR analysis of miR-760 expression in SW620 cells expressing miR-760 and control cells. [score:5]
Moreover, the expression of miR-760 decreased in a manner that correlated with Dukes stage, where the lowest expression of miR-760 was observed in later stages including both Dukes stage C and stage D patients (Fig. 1c). [score:5]
e Overall survival of 76 CRC patients who was stratified based on low expression (expression (>median, n = 38) of miR-760. [score:5]
These results indicate that miR-760 inhibits tumor progression in vivo by targeting BATF3 and downstream cyclin D1 -induced proliferation. [score:5]
In addition, BATF3 expression was found to be inversely correlated with miR-760 expression (r [2] = 0.68, P < 0.001, Fig. 7c). [score:5]
A total of 80 cases were used in this study to examine BATF3 expression, among which 45 samples were obtained from patients between 2014 and 2015 that tested positive for miR-760 expression, and the other 35 samples were obtained from patients in 2013 from the Department of Pathology at the First Affiliated Hospital of Zhengzhou University. [score:5]
Average miR-760 expression was normalized using U6 expression. [score:5]
Fig. 7The relationships between miR-760 expression and the expression of BATF3 in human CRC tissue. [score:5]
These results suggest that downregulation of miR-760 promotes the proliferation of CRC cells. [score:4]
As c-Jun was not in the predictive list of miR-760, we concluded that miR-760 directly targets BATF3 in CRC cells. [score:4]
Immunohistochemical assays were conducted to study the relationship between miR-760 expression and basic leucine zipper transcriptional factor ATF-like 3 (BATF3) expression in human CRC samples. [score:4]
miR-760 downregulation was significantly correlated with poor overall survival (P < 0.01). [score:4]
In summary, for the first time, our mechanistic study revealed BATF3 and Cyclin D1 genes as targets for miR-760, which underlies its role in CRC development. [score:4]
Here, we observed miR-760 directly targeted BATF3 and ectopic miR-760 resulted in a reduction of BATF3 and cyclinD1. [score:4]
Given the results of the integrated analysis, we hypothesize that decreased expression of miR-760 may promote CRC progression and development. [score:4]
MiR-760 targeted human BATF3 and inhibited cyclinD1 in CRC cells. [score:4]
We found lower expression of miR-760 in SW620 and DLD1 cell lines compared to miR-760 expression in HCT116 cells and the normal intestinal epithelium cell line FHC (Fig.   2a). [score:4]
In the present study, we found that miR-760 expression in tumor tissues was significantly reduced compared with healthy cells, and low miR-760 expression was associated with advanced Dukes stage and lymph node metastasis (Table 1). [score:4]
It has been reported that miR-760 is downregulated in CRC plasma, and has an 80.0% sensitivity and 72.4% specificity (AUC = 0.788) for the early detection of CRC, suggesting it can be used for early CRC diagnosis [31]. [score:4]
As shown in Fig. 3b and c, suppression of miR-760 by transfection with the miR-760 inhibitor significantly increased the growth rate of CRC cell lines when compared with cells innegative control (NC) group. [score:4]
Among these miRNAs, miR-760 was identified to be the most significantly downregulated gene in CRC tissues (Fig.   1a). [score:4]
CCK-8 assay indicated proliferation of SW620-psico (c), and HCT116-miR-760 -inhibitor cells (f) transfected with BATF3-siRNA were significantly inhibited compared with negative control (siNC). [score:3]
Finally, we showed a significant correlation between miR-760 and BATF3 expression in CRC tissues. [score:3]
Furthermore, it is likely that other molecules or signaling pathways will be discovered that are also targeted by miR-760 in CRC. [score:3]
Moreover, low miR-760 expression can predict poorer outcomes in CRC patients. [score:3]
g Schematic diagram of the proposed molecular mechanismby which miR-760 modulates CRC cell proliferationMoreover, we analyzed BATF3 expression levels in paired CRC samples randomly selected from previous 45 patients (n = 20) using qRT-PCR and. [score:3]
Collectively, miR-760 suppresses tumor cell growth in vitro and tumorigenesis in vivo. [score:3]
The expression levels of BATF3 in SW620-psico (a, b), and HCT116-miR-760 -inhibitor cells (d, e) which were transfected with BATF3-siRNA, as measured by Real-time PCR (a, d) and western blotting (b, e). [score:3]
c Photographs of the dissected tumors from miR-760 -overexpressed group and control group. [score:3]
g Schematic diagram of the proposed molecular mechanismby which miR-760 modulates CRC cell proliferation Moreover, we analyzed BATF3 expression levels in paired CRC samples randomly selected from previous 45 patients (n = 20) using qRT-PCR and. [score:3]
Fig. 1Expression of miR-760 was decreased in colorectal cancer tissues and cell lines. [score:3]
Elshafei A, Shaker O, Abd El-Motaal O, Salman T. The expression profiling of serum miR-92a, miR-375, and miR-760 in colorectal cancer: an Egyptian study. [score:3]
Additionally, Kaplan-Meier and Cox’s proportional hazards regression mo del survival analysis revealed that patients with low expression levels of miR-760 had shorter overall survival (Fig. 1e). [score:3]
Because CFSE and GFP are detected through the same fluorescent channel, we used miR-760 -mimic transduction to overexpress miR-760 (Fig. 2e, left). [score:3]
The results further support an inverse relationship between miR-760 and BATF3 expression in human CRC tissue. [score:3]
Taken together, the expression of miR-760 in tumors might serve as a prognostic marker for CRC patients. [score:3]
Next, we measured mature miR-760 expression levels in a paired independent validation sample cohort (n = 76) by qRT-PCR and normalized the results to U6 expression. [score:3]
Low miR-760 expression was observed in CRC tissues and was associated with poor prognosis. [score:3]
Using a median value of miR-760 expression as a cutoff point, we divided the samples obtained from 45 CRC patients with available miR-760 data into two groups. [score:3]
Moreover, miR-760 was found to reduce the cancer stem cell population and inhibit breast cancer cell proliferation and metastasis via inactivation of NANOG transcription factor [36]. [score:3]
We identified miR-760 as a clinically noteworthy miRNA in CRC and confirmed that miR-760 expression was lower in CRC patients with poor survival. [score:3]
Fig. 3Inhibition of miR-760 promoted the proliferation of colorectal cancer cells. [score:3]
c miR-760 was inversely associated with intratumoral BATF3 expression (r [2] = 0.68, P < 0.001). [score:3]
microRNA-760 (miR-760) Colorectal cancer (CRC) Basic leucine zipper transcriptional factor ATF-like 3 (BATF3) Activator protein 1 (AP-1) Colorectal cancer (CRC) is one of the most common malignancies in the world, with high rates of incidence and disease-related mortality and morbidity [1]. [score:3]
The results showed that miR-760 suppressed luciferase activity for the reporter plasmid carrying WT BATF3 3′-UTR, but no significant effects were observed for the reporter plasmid carrying mutant BATF3 3′-UTR (Fig. 4c, P< 0.05). [score:3]
In the present study, we found that miR-760 is dramatically downregulated in human CRC tissues compared with normal colorectal tissues. [score:3]
This analysis revealed BATF3 as a potential target of miR-760 (Fig.   4a). [score:3]
Our study may serve as a rational for targeting the miR-760/BATF3 interaction in a novel therapeutic application to treat CRC patients. [score:3]
After infection, GFP -positive cells were sorted by MoFloXDP (Beckman, USA) to obtain miR-760 overexpression cells. [score:3]
Clinical relevance of miR-760 and BATF3 expression in CRC. [score:3]
Statistical analyses showed that lower miR-760 expression was significantly associated with more advanced Dukes stages (P = 0.037) and lymph node metastasis (P = 0.036; Table  1). [score:3]
d reveals proliferation of HCT116 cells transfected with miR-760 inhibitor or negative control. [score:3]
Subsequently, expression levels of miR-760 were quantified by qRT-PCR using SYBR Premix ExTaqII (TaKaRa, Japan) in Agilent Mx3005P. [score:3]
b Schematic representation of miR-760 target sites in the 3′-UTR of BATF3 mRNA and a miR-760 mutant containing seven altered nucleotides in the seed sequence (mutant BATF33′-UTR). [score:3]
Interestingly, we also found that loss of miR-760 accelerated the expression of BATF3, c-Jun (Additional file 2: Figure S4), cyclin D1 (Fig. 4f) and vice versa. [score:3]
a miR-760 expression in human colorectal mucosa cell line FHC, and in CRC cell lines, including DLD1, HCT116, and SW620. [score:3]
e (left) Real-time PCR analysis of miR-760 expression in SW620 cells transfected with miR-760 mimic or negative control. [score:3]
BATF3 suppression was critical for miR-760 -induced cell proliferation in CRC cells. [score:3]
Together, these results suggest that miR-760 expression is decreased in human CRC cells. [score:3]
d Expression of miR-760, BATF3 and cyclin D1 mRNA in tumors. [score:3]
Therefore, miR-760 significantly inhibits the tumorigenesis of SW620 cells in vivo. [score:3]
b Real-time PCR analysis of miR-760 expression in paired fresh tissues from 76 CRC patients. [score:3]
The miRNA with the lowest expression, miR-760, was validated in an independent validation sample cohort of 76 CRC tissues. [score:3]
miR-760 overexpressed in SW620 or HCT116 cells, and control psico-transducted cells were seeded separately in 24-well plates and co -transfected with 0.25 μg pGL4.27-cyclinD1-WT plasmids, 0.25 μg pRL-TK plasmids (Promega), and 1 μl Lipo3000 (Invitrogen) in 50 μl Opti-MEM Reduced-Serum Medium following the manufacturer’s instructions. [score:3]
CCK-8 and colony formation assays revealed that overexpression of miR-760 dramatically decreased the growth rate of CRC cells (Fig. 2c and d). [score:2]
d Expression profiling of miRNAsreveals that miR-760 is lower in tumor tissues compared with normal tissues (n = 441, P < 0.0001; TCGA). [score:2]
MiR-760 suppressed tumor growth in vivo. [score:2]
MiR-760 inhibited proliferation and colony formation of CRC cells. [score:2]
We observed that growth rate was substantially decreased in miR-760 -overexpressing CRC cells compared with the growth rate of control cells (Fig. 2e, right). [score:2]
Additionally, showed the proliferation of miR-760 -inhibitor -transfected HCT116 cells increased compared to that of NC -transfected cells (Fig. 3d). [score:2]
Thus, miR-760 plays an essential role during the regulation of BATF3 in CRC cells in vitro and in vivo. [score:2]
As there are few reports that have explored the function of miR-760 in cancer and the precise role of miR-760 in cancer pathogenesis and progression remains unclear and even controversial, more studies are needed to determine the exact role and mechanism of miR-760 in tumor development. [score:2]
The results showed that miR-760 expression in tumors was significantly (P < 0.05) reduced compared to distant healthy tissues (Fig. 1b). [score:2]
MiR-760 inhibited human BATF3/c-Jun and downstream cyclin D1 in CRC cells. [score:2]
from the CCK-8 assay revealed that silencing BATF3 in SW620-psico and miR-760 inhibitor -transfected HCT116 cells increased the growth rate of the cells (Fig. 5c, f). [score:2]
SW620-psico or SW620-miR-760 cells (5 × 10 [6]) were suspended in 150 μl PBS and inoculated subcutaneously into the flanks of the nude mice. [score:1]
Xenograft nude mouse mo dels were used to determine the role of miR-760 in CRC tumorigenicity in vivo. [score:1]
However, there is also evidence to suggest that miR-760 acts as an onco-miR in certain cancer types. [score:1]
Future work will focus on revealing additional functions of miR-760 in CRC carcinogenesis and progression. [score:1]
The miR-760 gene was PCR-amplified from genomic DNA and cloned into a psicoR-GFP lentiviral vector. [score:1]
Psico-miR-760 was cotransfected with the psPAX2 and pMD2. [score:1]
293T cells were seeded in a 24-well plate and co -transfected with 0.5 μg pmirGLO vector, 80 nM of miR-760 -mimic and 1 μl of Lipo3000 (Invitrogen) in 50 μl of Opti-MEM Reduced-Serum Medium (Invitrogen). [score:1]
BATF3 harbors conserved miR-760 cognate binding sites, namely WT BATF33′-UTR (Fig. 4b). [score:1]
Mice with SW620-miR-760 tumors had smaller tumor size (P < 0.001, Fig.   6a) and less tumor weight (P < 0.01, Fig. 6b) than those with SW620-psico tumors (Fig. 6a, c). [score:1]
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2
[+] score: 9
The 6 upregulated miRNAs (mmu-miR-5132-5p, mmu-miR-3104-5p, mmu-miR-669c-5p, mmu-miR-705, mmu-miR-760-3p, mmu-miR-1962) and the 9 downregulated miRNAs (mmu-miR-146a, mmu-miR-138, mmu-miR-5123, mmu-miR-196b, mmu-miR-5099, mmu-miR-150, mmu-miR-145, mmu-miR-27a, mmu-miR-23a) chosen for validation were also based on their target genes predicted, whose functions are well relevant to inflammation and cancer. [score:9]
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3
[+] score: 7
For example, miR-760 and miR-424 were down-regulated, whereas miR-618, miR-570, and miR-107 were up-regulated upon the E2 stimulation [50]. [score:7]
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4
[+] score: 7
miR-522, miR-139-3p, miR-520c-5p, miR-518d-5p, miR-146b-5p, miR-34a, miR-526a, miR-193a-3p, miR-221, miR-4674 were significantly upregulated and miR-760 was downregulated in ECSCs (Figure 2A). [score:7]
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5
[+] score: 6
For example, it is known that human miR-186-5p, miR-216b-5p, miR-337-3p, and miR-760 cooperatively induce cellular senescence by targeting the gene CSNK2A1 in human colorectal cancer cells [35]. [score:3]
MiR-186, miR-216b, miR-337-3p, and miR-760 cooperatively induce cellular senescence by targeting α subunit of protein kinase CKII in human colorectal cancer cells. [score:3]
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6
[+] score: 5
Such as miR-100, miR-124, miR-206, miR-5116 and miR-760 were significantly downregulated in ICAM-1 knockout mouse lung tissue. [score:5]
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