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3 publications mentioning mmu-mir-710

Open access articles that are associated with the species Mus musculus and mention the gene name mir-710. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 14
Whilst plating the cells, 2 μl of 50 μM stock concentration of non -targeting miRNA -negative mimic, cel-miR-39-3p, miR-15b -mimic and mmu-miR-710 were added to 8 μl of antibiotic free RPMI1640 medium and the miRNAs were incubated for 5 minutes in tube 1. In tube 2, 0.5 μl of DharmaFECT3 was added to 9.5 μl of antibiotic free RPMI1640 medium. [score:3]
Non-specific cel-miR-39-3p control or mmu-miR-710 do not harbour the predicted target binding site for STIM1/2 or Orai1 and therefore, did not affect SOCE (Figure 6). [score:3]
T cells were transfected with miRNA -negative mimic (#479903), cel-miR-39-3p (#479902), miR-15b -mimic (#471275) and mmu-miR-710 (#470077) (all miRNAs mimics, control and inhibitors from Exiqon, Denmark) using DharmaFECT3 (#T-2003-01, Dharmacon, USA) as recommended by manufacture's gui delines. [score:3]
D. Original Western blots (left panels) and E. arithmetic means ± SEM, (n = 3, right panels) of STIM2/GAPDH and Orai1/GAPDH protein abundance in murine CD4 [+] T cells in negative mimic control (green bars), cel-miR-39-3p (blue bars), miR-15b mimic (red bars) and non-specific mmu-miR-710 (violet bars) transfected murine CD4 [+] T cells. [score:1]
B., C. Arithmetic means ± SEM (n = 4) of (B) STIM2/GAPDH and (C) Orai1/GAPDH transcript levels in negative mimic control (green bars), cel-miR-39-3p (blue bars), miR-15b mimic (red bars) and non-specific mmu-miR-710 (violet bars) transfected murine CD4 [+] T cells. [score:1]
After 20 minutes of incubation, the reaction mixture from tube 2 was added to corresponding wells to negative mimic, cel-miR-39-3p, miR-15b -mimic and mmu-miR-710 wells. [score:1]
B. Arithmetic means ± SEM (n = 3-6) of the slope (left) and peak (right) of the fluorescence ratio change following re-addition of extracellular Ca [2+] in negative mimic control (green bars), cel-miR-39-3p (blue bars), miR-15b mimic (red bars) and non-specific mmu-miR-710 (violet bars) transfected murine CD4 [+] T cells. [score:1]
A. Representative tracings showing the 340/380 nm fluorescence ratio reflecting cytosolic Ca [2+] activity in Fura-2/AM loaded negative mimic control (green curve), cel-miR-39-3p (blue curve), miR-15b mimic (red curve) and non-specific mmu-miR-710 (violet curve) transfected murine CD4 [+] T cells following exposure to Ca [2+]-free HEPES, additional exposure to Tg (1μM) and re-addition of extracellular Ca [2+] (Ca [2+] Std HEPES). [score:1]
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2
[+] score: 2
This filtering strategy allowed us to identify 2 genes (Ell2 and Entpd1) as well as 3 miRNAs (mir466, miR1187 and miR710) that are regulated by epigenetic factors and nuclear hormone receptors and are associated with AMD pathogenesis. [score:2]
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3
[+] score: 2
B) The level of let-7e, miR-212-3p, miR-546 and miR-710 in exosomes from MIN6B1 treated or not with cytokines for 48 h cells was determined by qPCR. [score:1]
For example, miR-546 and miR-710 were increased in response to cytokines whereas let-7e and miR-212-3p were more abundant in exosomes of untreated MIN6B1 cells (see Additional file 3: Figure S2B for confirmation of these results by qPCR). [score:1]
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