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5 publications mentioning bmo-mir-31

Open access articles that are associated with the species Bombyx mori and mention the gene name mir-31. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 230
Since microRNAs invariably seem to suppress the expression of their putative targets, miR-31 may target an unknown transcript which in turn negatively regulates the expression of Bim under constitutive condition. [score:12]
Therefore, our study confirms beyond doubt that miR-31 overexpression mediates Bax -dependent cell death through indirect induction of Bim overexpression by suppressing an intermediate gene/protein. [score:8]
In order to confirm whether overexpression of Sf-miR-31 has a functional role in the induction of radiation -induced Sf9 cell death, its expression was suppressed using antisense RNA prior to irradiation, and alteration in cell death induction was studied using morphological scoring (necrotic/apoptotic cells), flow cytometry (sub-G1 population) as well as by caspase-3 activity. [score:7]
Quite important, radiation -induced overexpression of Sf-miR-31 at doses >1,000Gy resulted in significant inhibition (p < 0.05) of cell death when it was inhibited/compromised using antisense miR-31 (AS-miR-31) (Fig. 3). [score:7]
Inhibition of Sf-miR-31 using antisense significantly inhibited Bax translocation induced by high doses, which implied an apparently indirect regulation of Bax -mediated processes by miR-31 (Fig. 5c,a). [score:7]
Importantly, miR-31 mimic -induced apoptosis also involved the upregulation of Bim expression (Fig. 7e). [score:6]
Following this, our results showing upregulation of miR-31 in cells irradiated at lethal doses indeed indicated its mediatory role in apoptosis, which was consequently confirmed by significant alteration (inhibition) of cell death by the ectopically administered AS-miR-31(*P < 0.05, **P < 0.02) (Fig. 3). [score:6]
We are presently looking for these putative targets of miR-31 that could be regulating Bim expression. [score:6]
Pro-apoptotic role of miR-31 is further clear from the fact that at sub-lethal doses (<500Gy), its expression was significantly downregulated (Fig. 2c). [score:6]
As shown in Fig. 6c,d, treatment with AS-miR-31 indeed caused inhibition of radiation -induced Bim overexpression and Bim/Bax interaction, confirming miR-31’s role in regulating Bim-Bax pathway. [score:6]
Interestingly, the Sf-miR-31 expression was significantly downregulated at these sub-lethal doses (p < 0.05). [score:6]
Interestingly, this study also implies that miR-31 downregulation could result in enhanced cellular radioresistance, since sub-lethal radiation doses were associated with considerable reduction in its expression level. [score:6]
Therefore, Bim upregulation may be consequently induced through miR-31 overexpression in different stress responses. [score:6]
Suppression of Sf-miR-31 inhibits radiation -induced cell death. [score:5]
A nearly four-fold increase in miR-31 expression was sufficient to cause ~20% increase in apoptotic cell death, which was essentially mediated through Bim overexpression, Bax translocation and cytochrome-c release into cytosol (Fig. 7). [score:5]
Since radiation induced significant overexpression of miR-31 which in turn mediated Bim-Bax pathway, we further confirmed the pro-apoptotic role of miR-31 by ectopically enhancing its expression in the unirradiated cells. [score:5]
Since AS-miR-31 was able to significantly inhibit the induction of Bax translocation as well as apoptosis, we tested if radiation -induced Bim overexpression is also altered in this condition. [score:5]
Radiation -induced miR-31 overexpression enhances Bim expression as well as its interaction with Bax. [score:5]
We further found that this radiation -induced Bim-Bax interaction is effectively inhibited by AS-miR-31, and so is the radiation -induced Bim overexpression (Fig. 6c,d). [score:5]
Predicted targets of Sf-miR-31 majorly include regulatory pathways of apoptotic signaling, among few other important cellular functions. [score:4]
Cell death/apoptosis regulation and stress signaling are among the important predicted target pathways of Sf-miR-31. [score:4]
Target prediction for miR-31 using PITA executable software showed many important genes that are important for stress signaling, cell death, cellular and organelle development as well as cellular communication (Supplementary Table 1). [score:4]
Secondly, these radiation -induced alterations in Bim-Bax ultimately leading to Bax translocation were dependent on miR-31 upregulation through yet unknown mediators. [score:4]
An isolated report has earlier shown upregulation of Bim by miR-31 albeit in unirradiated cells 13. [score:4]
Ectopic overexpression of miR-31 induces apoptosis in Sf9 cells and confirms its mediatory role in cell death. [score:3]
Inhibition of miR-31 decreases caspase -dependent Sf9 apoptosis. [score:3]
Pre-treatment with AS-miR-31 also failed to alter Bax and Bcl-2 expression/ratio (Fig. 4b). [score:3]
These 3′ UTRs were then queried against Bombyx mori miR-31 (Bmo-miR-31) for target accessibility and interaction using PITA executable program 41. [score:3]
As miRNA functions could be evolutionarily conserved between species such as B. mori and D. melanogaster 43, we used Flybase IDs of identified targets for the GO term clustering using Panther and Cytoscape 44 (Bingo) tools for the functional analysis of Sf-miR-31. [score:3]
On the other hand, a dose -dependent increase in caspase-3 activation and cell death marked by typical apoptotic bodies (Fig. 2a,b) was evident by 24 h following irradiation at higher doses (1,000Gy–3,000 Gy), which was associated with a concomitant increase in the expression of Sf-miR-31 (p < 0.002; Fig. 2c). [score:3]
Ectopic overexpression of Sf-miR-31 using miR-31 mimic sequence induced significant cell death in a Bim-Bax dependent manner. [score:3]
Lethal doses also caused release of cytochrome-c from mitochondria into cytosol following irradiation at 1,000Gy–3,000Gy and inhibiting Sf-miR-31 resulted in its partial but significant reduction (Fig. 5c). [score:3]
Radiation induced Bax translocation in the absence of Bax/Bcl-2 alterations is mediated by miR-31 overexpression. [score:3]
The expression of miR-31 after antisense and mimic miR-31 was quantified using Real Time PCR (Supplementary Fig. 2). [score:3]
Bax and Bcl-2 expression/ratio remains unaltered following irradiation or AS-miR-31 transfection. [score:3]
For target gene prediction of miR-31, we extracted 3′ UTRs of the Bombyx mori (silkworm, Dazao strain) from NCBI UniGene database (http://www. [score:3]
Radiation -induced changes in the expression of Sf-miR-31 were observed with real time PCR. [score:3]
Significant reduction in apoptosis is evident after Sf-miR-31 inhibition using antisense RNA at doses 2000Gy-3000Gy (16h–20 h post irradiation). [score:3]
In the right lane, upper band shows undigested probe and lower band depicts 21 bp mature miR-31; (c) Sf-miR-31 sequence was queried against all the predicted UTRs of Bombyx mori and the predicted potential targets were mapped using panther tool for analysis of molecular functions and biological processes. [score:3]
Further, administering AS-miR-31 could inhibit radiation -induced cell death significantly but partially (Fig. 3). [score:3]
Alteration in Sf-miR-31 expression corresponds with radiation -induced caspase activation and apoptosis. [score:3]
These results thus show that Bax translocation and consequent cytochrome-c release/cell death are partly regulated by Sf-miR-31. [score:2]
This indirect relationship between miR-31 and Bim was further corroborated when we treated Sf9 cells with the mimic sequences of miR-31. [score:2]
2 μl of cDNA was used to analyze the Sf-miR-31 expression using Sf-miR-31 specific forward primer in Real Time Thermocycler (Stratagene, MX3005P). [score:2]
The four major biological pathways predicted as regulated by Sf-miR-31 are shown in different groups within the hollow boxes. [score:2]
Functional annotation analysis further predicted participation of Sf-miR-31 in various cellular functions including the regulation of apoptosis (Fig. 1c,d; Supplementary Fig. 3 and Supplementary table 1). [score:2]
This study presents significant evidence to demonstrate that microRNA-31, which has been recently reported to regulate tumour radiosensitivity 19, is a prominent mediator of radiation -induced cell death in higher eukaryotes. [score:2]
These in-silico predictions hence strongly suggest Sf-miR-31 as a regulator of cell death/apoptosis. [score:2]
Since miR-31 is also known to be highly conserved from insects to mammals and regulates many cellular responses/functions, the present study provides a valuable insight into the potential role of this microRNA in the cellular radiosensitivity of higher eukaryotes. [score:2]
Even more important, the study demonstrates that radiation -induced miR-31 alters Bim -mediated regulation of Bax translocation that in turn results in the post-irradiation mitochondrial cytochrome-c release and apoptosis. [score:2]
miR-31 dependent translocation of Bax from cytosol to mitochondria corresponds with cytochrome-c release. [score:1]
Mere transfection of Sf9 cells with the miR-31 mimic sequence oligos could induced apoptotic cell death. [score:1]
Gene Ontology (GO) term clustering of Bmo-/Sf-miR-31 using Panther and Cytoscape (Bingo) tools suggested involvement of Sf-miR-31 in diverse cellular processes (Fig. 1c,d). [score:1]
How to cite this article: Kumar, A. et al. Evidence for microRNA-31 dependent Bim-Bax interaction preceding mitochondrial Bax translocation during radiation -induced apoptosis. [score:1]
ClustalW analysis of these pre-microRNA-31 sequences showed near–complete conservation within the mature microRNA-31 (miR-31) region across various orders including Lepidoptera (Fig. 1a). [score:1]
For transfection of AS-miR-31 and mimic miR-31, 0.50 μg of each was used with RNAiFect (Qiagen, Cat No. [score:1]
To test this, we used Bombyx mori miR-31 (order Lepidoptera) as a reference sequence for generating biotinylated probes for Sf9 (Fig. 1b). [score:1]
Cells were irradiated with and without previous transfection with AS-miR-31 and harvested 18 h post-irradiation. [score:1]
Identification and functional annotation of miRNA-31 in Sf9 cells. [score:1]
We detected miR-31 in Sf9 cells using in silico and experimental steps that involved in vitro transcription and generation of biotinylated probe, despite the fact that complete transcriptome database was not available for the organism Spodoptera including the miRNA sequences. [score:1]
Approximately four-fold increase in miR-31 level (using real-time PCR) following transfection with miR-31 mimics resulted in ~20% increase in cell death (Fig. 7a), which was associated with increase in caspase-3 activity (Fig. 7b) as well as significant mitochondrial Bax translocation and cytochrome-c release([*,#]P < 0.02) (Fig. 7c,d). [score:1]
Transfection control (TC) and scrambled antisense control (ASC) were used to confirm the specific effects of antisense-miR-31. [score:1]
Identification of Sf-miR-31 and predicting its putative physiological role. [score:1]
Construction of probe for the Sf-miR-31 identification was done using Bombyx mori mature miR-31 as a template for in vitro transcription. [score:1]
To study Bax translocation using immunofluorescence microscopy, cells were grown on cover slips in a 35 mm culture dish 24 h prior to transfection (with AS-miR-31 or MM-miR-31) and/or irradiation. [score:1]
Together, our results confirm the existence of miR-31 as well as its mediatory role in radiation -induced cell death in the radioresistant Sf9 cells. [score:1]
Radiation -induced caspase-3 dependent cell death coincides with Sf-miR-31. [score:1]
In brief, cells were harvested 24h following irradiation with or without transfection with AS-miR-31. [score:1]
Sub-lethal (200Gy) and lethal (2000Gy) radiation doses were used with and without prior treatment with AS-miR-31. [score:1]
Since our in silico analysis showed that mature miR-31 sequence is perfectly conserved between as well as within different insect orders (Fig. 1a), we used the Bombyx mori sequence for designing these biotinylated probes (as detailed in the methods section) for detecting miR-31 in the RNA pool of Sf9 cells. [score:1]
Therefore, we extracted pre-miRNA-31 sequences from different orders of class Insecta using the microRNAs database (miRbase. [score:1]
In vitro probe construction and microRNA identificationConstruction of probe for the Sf-miR-31 identification was done using Bombyx mori mature miR-31 as a template for in vitro transcription. [score:1]
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2
[+] score: 13
Bmo-miR-31, bmo-miR-71, and bmo-miR-77 displayed a very similar expression pattern, higher expression at NLS (p = 0.027, 0.035 and 0.0064, respectively); bmo-miR-31 and bmo-miR-77 are also expressed at a higher level at MLS than at other stages (p = 0.019 and 0.0082, respectively). [score:7]
According to our results, bmo-miR-31, its homolog dme-miR-31a is expressed in a pair-rule pattern of 14 stripes and in the foregut, anterior endoderm, and hindgut [42], and miR-31 was also found to have a high expression level in the small intestine of mammals [75], which contributes to the tumorigenesis and the acquisition of a more aggressive phenotype in human colorectal cancer [76]. [score:5]
Those findings provided clues that bmo-miR-31 at MLS may control epithelial metabolism during molting. [score:1]
[1 to 20 of 3 sentences]
3
[+] score: 4
miR-100 (5 fold), miR-31 (3 fold) and miR-184 (3 fold) were expressed at much higher levels in moth relative to other stages. [score:3]
It includes miR-1, the entire family of miR-2, the miR-9 family (miR-9 and miR-9b), the let-7 family (let-7a, let-7j), miR-10b, miR-31, miR-71, miR-79, miR-87, miR-98, miR-100, miR-252, miR-263a, miR-275, miR-279, miR-317 and miR-1274b (Table 3 and Figure 4a). [score:1]
[1 to 20 of 2 sentences]
4
[+] score: 3
bmo-let-7b, bmo-let-7c, bmo-miR-9, bmo-miR-9*, bmo-miR-100-like, bmo-miR-263a, bmo-miR-31 and bmo-bantam were expressed in larva and pupa, but were not detected in moth; of these miRNAs, bmo-miR-9 and bmo-miR-9* are also complementary miRNAs. [score:3]
[1 to 20 of 1 sentences]
5
[+] score: 1
Further analysis suggested that conserved miRNAs such as bmo-bantam, bmo-let-7, bmo-miR-31, bmo-miR-8 were in general highly abundant. [score:1]
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