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34 publications mentioning bta-mir-103-2

Open access articles that are associated with the species Bos taurus and mention the gene name mir-103-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 473
Interestingly, we found that over -expression of miR-103 using Ad-miR-103 (adenovirus inserted with miR-103) down-regulates the expression of PANK3, whereas suppression of miR-103 leads to up-regulation of PANK3 (Figure 3B, C) by using miR-103-antisense -inhibitor, a small, chemically modified single-stranded RNA molecule designed to specifically bind to and inhibit endogenous miRNA molecules. [score:17]
Over -expression of miR-103 down-regulates the expression of PANK3 in GMEC, whereas suppression of miR-103 leads to up-regulation of PANK3. [score:13]
Based on the similar expression profiles of transcriptional factors and their downstream targets, we speculated that up-regulation of genes associated with the milk fat synthesis in miR-103 over -expression background may be due to the increased expression of PPARγ, SREBP-1c, and LXRα in GMEC. [score:12]
Goat specific miR-103 -inhibitor (antisense inhibitor for miR-103) (Anti-miR™ miRNA Inhibitor) and inhibitor negative control antisense oligonucleotide (Anti-miR™ miRNA Inhibitor Negative Control [#]1) were purchased from Invitrogen (USA). [score:11]
The levels of PDK4, glutamate dehydrogenase 1(GUD1, data not show), ACSL1 and ACOX1 were decreased as miR-103 over-expressed, and their expression were increased as miR-103 expression suppressed (data not show). [score:9]
The up-regulation of LPL and SLC27A6 are in accordance with the increased total fatty acid content (Figure 4D) and up-regulated C16:0 content (Figure 4F), suggesting that a higher level of fatty acid utilization is triggered by augmented miR-103 expression. [score:9]
For triglyceride synthesis, expression of DGA1 (1.21-fold, p<0.05) and S CD (2.87-fold, p<0.05) was up-regulated with elevated miR-103 expression, supporting our previous findings that triglyceride content and unsaturated fatty acids content were both increased in epithelial cells (Figure 4C, E). [score:8]
Through suppression of ACSL1 and ACOX1 expression, miR-103 may decrease the whole β-oxidation level and up-regulate acetyl-CoA amounts for fatty acid and triglyceride synthesis to meet the huge milk demands needed during mid-lactation. [score:8]
Over -expression or suppression of miR-103 was conducted by using Ad-miR-103 or miR-103 inhibitor as controls, respectively. [score:7]
Figure S3 MiR-103 expression in GMEC treated with Ad and inhibitor control (treatments are Ad-miR-103 and miR-103 inhibitor). [score:7]
Over -expression of miR-103 increases the mRNA expression level of LEP (A), whereas decreases the mRNA expression level of AMPKα (B). [score:7]
Relative mRNA expression in miR-103 over -expression or suppression background. [score:7]
In contrast, to the increased milk fat resulting from miR-103 over -expression, suppression of miR-103 expression had no effect on fat droplet accumulation (data not show). [score:7]
PPARγ, peroxisome proliferator-activated receptor γ; SREBP-1c, sterol regulatory element -binding protein-1c; LXRα,nuclear oxysterol receptorα;other gene symbols were listed in Table 1. Over -expression of miR-103 down-regulates the mRNA levels of HSL (A), ATGL (B), ACSL1(C), CPT1 (D), PPARα (E) and ACOX1 (F). [score:7]
QRT-PCR analysis (Figure S2) indicated that miR-103 over -expression significantly increased LEP mRNA expression relative to controls, while it decreased the expression of AMPKα, which is consistent with an increase in fatty acid content and a decrease in β-oxidation levels. [score:7]
Ectopic expression of miR-103 in preadipocyte 3T3-L1 cells up-regulated adipogenesis markers and increased triglyceride accumulation at an early stage of adipogenesis [47], while miR-103 silencing in OB/OB mice resulted in reduced levels of fat-pad weights [48]. [score:6]
Previous studies have shown that miR-103 is generally expressed in different tissues and cells [42], [43], [44], while differentially expressed during adipogenesis [45] and development [46]. [score:6]
*, p<0.05; C: Elevated miR-103 expression up-regulates triglyceride content. [score:6]
The entire gene network that controlls the milk fat synthesis, was up-regulated, supporting our previous findings that miR-103 over -expression increased the amount of fat droplets, as well as the contents of triglycerides and fatty acids. [score:6]
We compared miR-103 expression in mammary gland at two different physiological stages by quantitative Real-time PCR (qRT-PCR) and found the expression level of miR-103 was higher (4.3-fold, p<0.05) during the mid-lactation period than that in the dry period (Figure 2), suggesting that miR-103 may be involved in regulating lactation or development of the adult mammary gland. [score:6]
Fatty acids produced from lipolysis are transported by long-chain acyl-CoA synthetase 1 (ACSL1, a predicted target of miR-103 [Table S3]), enter into mitochondria by carnitine palmitoyltransferase (CPT1), and undergoes β-oxidation which is regulated by peroxisome proliferator-activated receptor α (PPARα) and acyl-CoA oxidase 1 (ACOX1, a predicted target of miR-103 [Table S3]) [41]. [score:6]
0079258.g006 Figure 6Over -expression of miR-103 down-regulates the mRNA levels of HSL (A), ATGL (B), ACSL1(C), CPT1 (D), PPARα (E) and ACOX1 (F). [score:6]
Furthermore, for fat droplet formation, the up-regulated expression of ADRP (18.02-fold, p<0.05) was consistent with the increased fat droplets in Ad-miR-103-infected cells (Figure 4B). [score:6]
Suppression of β-oxidation resulted in a down-regulation of energy supplies; however Ad-miR-103-infected cells still accumulated more triglycerides. [score:6]
And inhibitor-control doesn’t alter miR-103 expression by itself (Figure S3). [score:5]
MiR-103 was co-regulated with PANK and miR-103 also can regulate PANK expression though an unknown pathway. [score:5]
0079258.g005 Figure 5Elevated miR-103 expression promotes the expression levels of PPARγ (A), DGAT1(B), ABCA1(C), LXRα (D), ABCG1(E), SREBP-1c(F) FASN (G), and ACACA (H). [score:5]
The expression profiles of ACSL1, CPT1, PPARα, and ACOX1 were quite different; however their expression in Ad-miR-103-infected cells was lower than the Ad control (Figure 6C-F). [score:5]
MiR-103, one of the 30 most abundantly expressed miRNAs in the lactating mammary gland, controls gene expression (Figure 7), goat milk fat accumulation, as well as the ratio of unsaturated/saturated fatty acids. [score:5]
MiR-103 up-regulates gene expression associated with the milk fat synthesis process in GMEC. [score:5]
Over -expression of miR-103 transcriptionally hastens the expression of key transcription factors controlling the milk fat synthesis. [score:5]
We found that miR-103 had the lowest expression level with 60 nM inhibitor-control (Figure 3B). [score:5]
And the expression levels of miR-103 were determined at 48 h after transfecting GMEC with inhibitor-control. [score:5]
To validate miR-103 target genes, we measured the expression of miR-103 predicted targets (Table S3). [score:5]
SREBP-1c (Figure 5F) showed a different expression profile with FASN (Figure 5G) and ACACA (Figure 5H); however, the expression of these three genes in Ad-miR-103-infected cells was always greater than that of Ad-infected cells. [score:5]
Inhibitor control has no effect on miR-103 expression at any concentration. [score:5]
Elevated miR-103 expression decreases gene expression associated with lipolysis and β-oxidation. [score:5]
However, we have not yet obtained direct proof that one of these genes is a direct target of miR-103. [score:5]
Intensity scatter plot shows comparison of expression of miR-103 and PANK3; B: The optimal MOI (200) of Ad-miR-103 for infection and dose of miR-103 -inhibitor for transfection (60 nM). [score:5]
Elevated miR-103 expression promotes the expression levels of PPARγ (A), DGAT1(B), ABCA1(C), LXRα (D), ABCG1(E), SREBP-1c(F) FASN (G), and ACACA (H). [score:5]
Even so, we have used MFOLD software to analyze the ΔG of the 80 bp flanking sequence of a miR-103 binding site on these predicted targets, and have constructed miR-103 sensors using pGL3-control luciferase reporter vector inserted into the XbaI locus with 100-200 bp miRNA binding site of targets. [score:5]
One possible explanation for this is that other miRNAs in the miR-103 family (e. g., miR-107), or synergic miRNAs which target genes in conjunction with miR-103, could regulate fatty acid synthesis, compensating for miR-103 knockdown. [score:5]
However, miR-103 expression had little effect on the expression of JAK1 and other genes. [score:5]
Inhibitor-control was used as control for miR-103 -inhibitor-control. [score:5]
In the mammary glands of lactating goats, we found that miRNAs associated with cell proliferation (miR-26a, miR-21), conferring epithelial phenotype (miR-29a, miR-30a/d), immune response and development (miR-181, let-7a/b/f/g/i) were abundantly expressed, as well as miRNAs involved in lipid metabolism (miR-103, miR-23a, miR-27b, miR-200a/b/c). [score:4]
Gene expression in Ad(control)-infected, Ad-miR-103-infected, and uninfected cells was assessed at 0, 24, 48, and 72 h. qRT-PCR measurement of gene expression expressed as fold change compared to their respective level at 0 h, normalized to 1. Columns, average of 12 experiments; bars, SEM. [score:4]
Figure S2 MiR-103 increases LEP expression and decreases AMPKα expression. [score:4]
One possible explanation is that the suppression of miR-103 could be compensated by other redundant signaling pathways and thereby, knockdown of miR-103 alone does not affect fat droplet formation. [score:4]
Gene expression in Ad(control)-infected, Ad-miR-103-infected, and uninfected GMEC was assessed at 0, 24, 48, and 72 h. qRT-PCR measurement of gene expression expressed as fold change compared to their respective level at 0 h, normalized to 1. Columns, average of 12 experiments; bars, SEM. [score:4]
Genes associated with milk fat metabolism are shown to be positively (arrowheads) or negatively (end lines) regulated by miR-103 over-expressed. [score:4]
Gene expression in Ad-infected, Ad-miR-103-infected, and uninfected cells was assessed at 0, 24, 48, and 72 h. qRT-PCR measurement of gene expression expressed as fold change compared to their respective level at 0 h. Columns, average of 12 experiments; bars, SEM. [score:4]
0079258.g007 Figure 7 Genes associated with milk fat metabolism are shown to be positively (arrowheads) or negatively (end lines) regulated by miR-103 over-expressed. [score:4]
MOI of Ad-miR-103 and concentration of inhibitor is shown under X coordinate axis. [score:3]
The expression levels of miR-103 were determined at 72 h after infecting GMEC with Ad. [score:3]
To this end, we generated a recombinant adenovirus expressing miR-103 (Ad-miR-103), and investigated the effect of elevated expression of miR-103 on milk fat synthesis using goat mammary gland epithelial cells (GMEC). [score:3]
Additionally, no miR-103 binding sites were found in the 3′, 5′or the coding region of PANK3, indicating that this regulation is indirect. [score:3]
Over -expression of miR-103 can increase fat droplet, triglyceride, and fatty acid contents, which had been identified in the manuscript. [score:3]
We found that lipolysis in GMEC is not the main way to provide fatty acids for β-oxidation, for the mRNA expression of lipolysis-related genes (e. g., HSL and ATGL) are relatively low in normal and Ad-miR-103-infected cells (data not show). [score:3]
We presented novel data concerning miR-103 expression in lactating goats using a Solexa sequencing approach. [score:3]
Elevated miR-103 expression alters fatty acid composition in GMEC. [score:3]
MiR-103 expression was higher in Ad-miR-103-infected cells than that in Ad-infected cells, (2.42-fold, p<0.05, Figure 3B) at a MOI of 200. [score:3]
These transcription factors and their downstreams are not predicted targets of miR-103. [score:3]
Over -expression of miR-103 promotes milk fat droplet accumulation in GMEC. [score:3]
To investigate how miR-103 affects milk fat synthesis, we assessed the expression of key genes involved in these processes at 72 h in GMEC that over -expressing miR-103 (Table 1). [score:3]
A: Over -expression of miR-103 promotes fat accumulation. [score:3]
Ad can make a slight decrease in miR-103 expression at a MOI of ≥300. [score:3]
We compared the fat droplet formation between cells over -expressing miR-103 and controls that included cells infected by adenovirus (Ad) without any miRNA sequences and uninfected cells by using oil red O. With an optimal dose (multiplicity of infection: 200), we found that over -expression of miR-103 causes significantly increased fat droplet accumulation compared to Ad-control and uninfected cells (Figure 4A, B). [score:3]
In addition, some predicted targets of miR-103 (i. e., Long-chain acyl-CoA synthetase 1[ACSL1]) are involved in lactation [41]. [score:3]
*, p<0.05; E: Over -expression of miR-103 reduces total saturated fatty acids and promotes total unsaturated fatty acids. [score:3]
Transfection GMEC with antisense inhibitor of miR-103. [score:3]
For de novo fatty acid synthesis, expression levels of FASN and ACACA in Ad-miR-103-infected cells were significantly higher than in control groups (Ad-infected cells) (1.15-fold, p<0.05; 1.57-fold, p<0.05). [score:3]
*, p<0.05; F: Over -expression of miR-103 alters the composition of major types of fatty acids in GMEC. [score:3]
This correlation between miR-103-1 and PANK3 expression suggests that the function of miR-103-1 may be related to PANK3's; thereby miR-103 might modulate milk fat synthesis. [score:3]
Arrows indicate Ad- and Ad-miR-103-infected cells; B: Over -expression of miR-103 increases fat droplet content. [score:3]
We assessed the mRNA expression of these three transcription factors at 0, 24, 48, and 72 h in GMEC with Ad-miR-103 (Figure 5) (the data of Figure 5 (72 h) and Table 1 were generated from a same experiment). [score:3]
Then, an aliquot (500 µl) of medium in serum-and-antibiotic-free was added to 6-wells incubated with a complex compromising 20 µl transfection reagent, and 60 nM miR-103 -inhibitor or 60 nM negative control for 15 min according to procedure manual of Lipofectamine™ RNAiMAX (Invitrogen, USA). [score:3]
A possible explanation is that miR-103 may target other genes that can affect the transcriptional activity of PANK3. [score:3]
For fatty acid hydrolysis and uptake, expression of LPL and SLC27A6 in Ad-miR-103-infected cells was significantly greater than that of Ad-infected cells (20.48-fold, p<0.01; 1.40-fold, p<0.05). [score:3]
However, no significant differences were observed between the fat droplet accumulation in cells treated with the miR-103 -inhibitor and the negative control (data not show). [score:3]
In this study, we found ACSL1 and ACOX1, both involved in β-oxidation, to be predicted targets of miR-103 (Table S3). [score:3]
Table S3 Predicted targets of miR-103. [score:3]
0079258.g004 Figure 4A: Over -expression of miR-103 promotes fat accumulation. [score:3]
*, p<0.05; D: Over -expression of miR-103 increases total fatty acids content in GMEC. [score:3]
This result is consistent with previous studies done in the mouse 3T3-L1 cell line [47], suggesting that co-regulation of miRNA-103 and its host gene is highly conserved in mammals. [score:2]
The data (miR-103 levels) were expressed as fold change as compared to normal cells (MOI = 0 and 0 nM), normalized to 1. Columns, average of 3 experiments. [score:2]
Our data suggests that miR-103 plays an important role in regulating triglyceride synthesis. [score:2]
In summary, we have identified miR-103 as a new class of regulators of milk fat synthesis. [score:2]
The enrichment of miR-103 at the mid-lactation stage may be a reflection of its physiological role in the regulation of lactation. [score:2]
MiR-103 is differentially expressed in mid-lactation and dry period. [score:2]
From the top 30 miRNAs, we found six miRNAs (e. g., miR-23a, miR-27b, miR-103, miR-200a/b/c) to be related to lipid metabolism in human adipocyte cells: miR-23 enhances glutamine metabolism [38]; miR-27 decreases fat accumulation [27]; miR-103 regulates triglyceride content during cell differentiation [39]; and miR-200 affects insulin signaling [40]. [score:2]
MiR-103 is highly expressed in mid-lactation. [score:2]
The data (miR-103 levels) were expressed as fold change as compared to controls, normalized to 1. Columns, average of 12 experiments; bars, SEM. [score:2]
MiR-103 expression correlates with lactation stages. [score:2]
For the Ad control, miR-103 in Ad-infected cells (at a MOI of 200) did not change compared to uninfected cells (Figure S3), suggesting that Ad doesn’t alter miR-103 expression by itself at this MOI. [score:2]
Taken together, miR-103 plays a significant role in regulating fatty acid composition. [score:2]
Our results suggest that miR-103 may be an important regulator for fat composition and nutrient level of goat milk. [score:2]
MiR-103 may suppress β-oxidation to increase triglyceride content. [score:2]
Taken as a whole, miR-103 has an extensive role in regulating milk fat synthesis in goats. [score:2]
Our results indicate that miR-103 has a significant role in milk fat accumulation in goats. [score:1]
From abundantly expressed miRNAs, we chose miR-103, which has been reported to be involved in lipid metabolism in adipose tissue, to further investigate the correlation between this miRNA and lactation. [score:1]
Fat droplets were extracted using isopropanol in un-, Ad-control without any miRNA sequences and Ad-miR-103-infected cells at 72 h post infection. [score:1]
MiR-103 may decrease β-oxidation through regulating Leptin (LEP) and AMP-activated protein kinase subunit α (AMPKα) pathways. [score:1]
However, we were not able to analyze the relationship between miR-103-2, miR-107 and their host gene PANK2, PANK1, as the sequences of PANK1 and PANK2 in goat are unknown and cDNA cloning did not succeed. [score:1]
Specifically, triglyceride content of Ad-miR-103-infected cells was 33% higher (p<0.05) than that of Ad-infected cells. [score:1]
Ad-miR-103 generation and infection. [score:1]
Collectively, our data not only provides new insights regarding miRNAs participation in the gene network controlling milk fat synthesis, but also gives us important clues that miR-103 may be used as an index for a molecular breeding program in goats. [score:1]
The Ad-miR-103- and Ad-infected cells were harvested in lysis buffer (50 mmol/l Tris-HCL, pH 7.4, 150 mmol/NaCl, 1% Triton X-100) and sonicated to homogenize the cell suspension. [score:1]
The analysis of fatty acid contents showed that Ad-miR-103-infected cells accumulated more c9-C18:1 (1.35-fold, p<0.05), t11-C18:1 (1.17-fold) and c9,t11-C18:2 (2.16-fold, p<0.05) (Figure 3F). [score:1]
Of the six miRNAs evaluated, miR-103 is the most abundantly expressed miRNA (272,319) (Table S2). [score:1]
Gene networks modulated by miR-103. [score:1]
The MiR-103 family has three members, miR-103-1, miR-103-2 and miR-107, which reside in the sense oriented intron 5 of three members of the pantothenate kinase (PANK) gene family members across species: PANK3, PANK2, and PANK1, respectively. [score:1]
Triglyceride content was determined by using a Serum Triglyceride Determination Kit in un-, Ad-(control) and Ad-miR-103-infected cells. [score:1]
The Ad-miR-103- and Ad-infected cells were harvested and collected in sealable glass tubes. [score:1]
The Ad-miRNA-103- and Ad-infected cells cultured in wells (6 well plate) were rinsed three times in phosphate- buffered saline (PBS), fixed in 10% (v/v) paraformaldehyde for 40 min, and then rinsed again with PBS. [score:1]
For the first time we show that goat mammary gland-enriched miR-103 is linked to milk triglyceride accumulation and unsaturated fatty acids content, indicating that miRNAs may potentially play a role in milk production and the synthesis of beneficial milk components in dairy animal. [score:1]
Authentic miR-103 stem-loop and about 300 nucleotides flanking sequences on the 5′ and 3′ side of miR-103 were amplified from normal Xinong Saanen Dairy Goat genomic DNA. [score:1]
MiR-103 regulates milk fat synthesis. [score:1]
Thus, miR-103 was chosen for further functional studies. [score:1]
We harvested GMEC at 72h post-infection by Ad-(control) and Ad-miR-103, respectively. [score:1]
Therefore, miR-103 could be a candidate gene used for increasing milk yield or unsaturated fatty acid production in the goat milk industry, which is one of the main objectives of dairy goat breeding. [score:1]
GMEC was infected with Ad or Ad-miR-103 at multiplicities of infection MOI (MOI) of 50, 100, 150, 200 or 250. [score:1]
We used a triglyceride determination kit (Sigma-Aldrich) to determine the triglyceride content in GMEC at 72 h after infection with Ad-miR-103 virus. [score:1]
Ad(control)-infected cells were used as control for Ad-miR-103-infected cells. [score:1]
Triglyceride content of Ad-miR-103-infected cells was higher than that of uninfected and Ad-infected cells (Figure 4C). [score:1]
MiR-103 is differentially regulated at mid-lactation and dry period. [score:1]
The adenovirus vectors pAd-control(which dose not contain an inserted sequence) and pAd-miR-103 were constructed and packed in HEK 293 cells using a commercial system (AdEasy, Stratagene). [score:1]
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2
[+] score: 42
b In porcine oocytes and COCs the combination of three miRNAs was optimal, although the difference between using 2 or 3 miRNAs was negligibleExpression of the miRNAs miR-93 and miR-103 was subsequently used to normalize the expression levels of the other miRNAs and nuclear RNA analyzed in bovine samples (Fig.   3a-e). [score:5]
b In porcine oocytes and COCs the combination of three miRNAs was optimal, although the difference between using 2 or 3 miRNAs was negligible Expression of the miRNAs miR-93 and miR-103 was subsequently used to normalize the expression levels of the other miRNAs and nuclear RNA analyzed in bovine samples (Fig.   3a-e). [score:5]
qRT-PCR was performed to quantify expression of miR-93, miR-103, miR-26a, miR-191, miR-23b, Let-7a and U6 for bovine samples and miR-21, miR-26a, miR-93, miR-103, miR-148a, miR-182 and miR-191 for porcine oocytes. [score:3]
In bovine samples, the expression of miR-23b, miR-26a, miR-93, miR-103, miR-191, Let-7a and the nuclear RNA U6 was examined. [score:3]
Among the miRNAs tested for bovine oocytes and embryos, miR-93 and miR-103 showed similar and more stable expression patterns. [score:3]
The combination of miR-93 and miR-103 is optimal for normalizing miRNA expression for qPCR experiments on bovine oocytes and preimplantation embryos; the preferred combination for porcine oocytes is miR-26a, miR-191 and miR-93. [score:3]
Fig. 3Relative expression of bovine miRNAs after normalization with miR-93 and miR-103. [score:3]
In cattle, the BestKeeper outcome indicated expression levels of miR-93, miR-103 and miR-191 as having the highest coefficient of correlation [r] indicator of a linear relationship between two variables (Table  1). [score:3]
In these samples, expression of miR-26a, miR-191, miR-93 and miR-103 were most similar. [score:3]
Stepwise removal to determine the optimum number of reference miRNAs identified miR-93 and miR-103 as the most stably expressed in bovine samples and miR-26a, miR-191 and miR-93 in porcine samples. [score:3]
In cattle oocytes and embryos, miR-103 and miR-93 showed the lowest M value, and thus highest stability, whereas Let-7a and U6 expression was more variable (Fig.   1a). [score:3]
In porcine samples, the miRNAs identified as fluctuating least in bovine samples, namely miR-26a, miR-93, miR-103 and miR-191 were examined together with miR-21, miR-148a, and miR-182. [score:1]
miR-23b miR-26a miR-93 miR-103 miR-191 Let-7a U6 coeff. [score:1]
miR-21 miR-26a miR-93 miR-103 miR-148 miR-182 miR-191 coeff. [score:1]
In porcine samples, miR-93, miR-26a, miR-191 and miR-103 had the highest r and r [2] values (Tables  3, 4). [score:1]
The combination of miR-93 and miR-103 had the highest r and r [2] values (Table  1). [score:1]
[1 to 20 of 16 sentences]
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[+] score: 22
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, hsa-mir-26b, hsa-mir-27a, hsa-mir-31, hsa-mir-33a, hsa-mir-99a, hsa-mir-100, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-199a-1, hsa-mir-148a, hsa-mir-147a, hsa-mir-34a, hsa-mir-182, hsa-mir-199a-2, hsa-mir-212, hsa-mir-221, hsa-mir-224, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-130a, hsa-mir-132, hsa-mir-142, hsa-mir-145, hsa-mir-152, hsa-mir-153-1, hsa-mir-153-2, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-127, hsa-mir-134, hsa-mir-200c, hsa-mir-106b, hsa-mir-361, hsa-mir-148b, hsa-mir-20b, hsa-mir-410, hsa-mir-202, hsa-mir-503, hsa-mir-33b, hsa-mir-643, hsa-mir-659, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-21, bta-mir-221, bta-mir-26b, bta-mir-27a, bta-mir-99a, bta-mir-125a, bta-mir-125b-1, bta-mir-145, bta-mir-199a-1, bta-mir-27b, bta-mir-30b, bta-mir-31, bta-mir-127, bta-mir-142, bta-mir-20b, bta-let-7d, bta-mir-132, bta-mir-148b, bta-mir-200c, bta-mir-22, bta-mir-23a, bta-mir-29b-2, bta-mir-361, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-let-7f-1, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-125b-2, bta-mir-34a, hsa-mir-708, hsa-mir-147b, hsa-mir-877, hsa-mir-940, hsa-mir-548j, hsa-mir-302e, hsa-mir-103b-1, hsa-mir-103b-2, bta-mir-100, bta-mir-106b, bta-mir-130a, bta-mir-134, bta-mir-147, bta-mir-152, bta-mir-153-1, bta-mir-153-2, bta-mir-182, bta-mir-24-1, bta-mir-199a-2, bta-mir-202, bta-mir-212, bta-mir-224, bta-mir-33a, bta-mir-33b, bta-mir-410, bta-mir-708, bta-mir-877, bta-mir-940, bta-mir-29b-1, bta-mir-148c, bta-mir-503, bta-mir-148d
Relative expression of candidate circulatory miRNAs in blood plasma of hypersitimulated heifers across different days during estrous (a- f) Expression of miR-221, miR-103, let-7g, miR-134, miR-147 and miR-127-3p was determined by RT-qPCR and each miRNA expression level was normalized using global normalization method. [score:7]
As shown in Fig.   4 expression analysis of candidate miRNAs (miR-221, miR-103, let-7 g, miR-134, miR-147 and miR-127-3p) using qRT-PCR revealed the presence of temporal changes in the pattern of expression depending on the time during the estrous cycle as shown in Fig.   4. While four of the candidates namely. [score:5]
For instance, miR-221, miR-103, miR-134 and miR-127-3p show significantly higher expression at day 7 of estrous cycle compared to day 0 or day 3. In contrast, miR-147 has significantly lower expression at day 7 of estrous. [score:4]
miR-221, miR-103, miR-134 and miR-127-3p showed a significant increase in abundance at day 7 of the estrous cycle compared to days 0 and 3, miR-147 showed a decreasing pattern from day 0 to day 7. No significant difference in the expression of let-7 g miRNA has been observed between the days during estrous cycle. [score:2]
Data are presented as raw Ct value and Ct value of more than 35 was considered as undetected Here we have tried to detect candidate miRNAs, which were enriched (miR-221, miR-103 & let-7 g) or suppressed (miR-134, miR-147 & miR-127-3p) in blood plasma samples of hyperstimulated animals compared to the unstimulated ones. [score:1]
Mir-103 and miR-127-3p were not detected in the Ago2 protein fraction of both treatment groups. [score:1]
Candidates including miR-182 from follicular fluid (Fig.   4) and miR-221, miR-103 and miR-127-3p from blood plasma (Fig.   5) were found to be detected only in exosomal fraction and completely undetected in the Ago2 fraction. [score:1]
As shown in Fig.   7, all candidate miRNAs, except miR-103 and miR-127-3p, were detected in both exosomal and Ago2 fraction of blood plasma of both hyperstimulated and unstimulated heifers. [score:1]
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4
[+] score: 21
In the present study, miR-103 was upregulated in steers fed high fat diet suggesting that a change in dietary fat content may alter the miR-103 expression and play a regulatory role in bovine adipose tissue. [score:7]
This miRNA was found to bind mRNA of caveolin-1, a factor that regulates insulin sensitivity, with the upregulation of miR-103 in liver or fat tissue being associated with impaired glucose homeostasis in mice [41]. [score:5]
Among those, we detected well studied miRNAs including miR-103 which has a pro-adipogenic role [33], miRNA let-7, reported to have an anti-adipogenic role in 3T3-L1 adipocyte cell culture regulating HMGA2 [34] and miR-27b which regulates the expression of PPARγ, considered the master regulator of adipogenesis [35]. [score:5]
Six miRNAs (miR-16b, miR-19a, miR-106, miR142–5 p, miR-196a and miR-2454) had the expression in agreement between qRT-PCR and microarray, however the other six (miR92a, miR-92b, miR-101, miR-103, miR-296 and miR-2368*) had divergent results. [score:3]
A study on humans showed that miR-103 was the most stable miRNA under different variable conditions, including fat tissue location, body mass index, physiological vs. [score:1]
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5
[+] score: 19
Other miRNAs from this paper: bta-let-7f-2, bta-mir-101-2, bta-mir-103-1, bta-mir-21, bta-mir-27a, bta-mir-30d, bta-mir-320a-2, bta-mir-199a-1, bta-mir-27b, bta-mir-30b, bta-mir-107, bta-mir-140, bta-mir-30e, bta-let-7d, bta-mir-124a-1, bta-mir-199b, bta-mir-200a, bta-mir-200c, bta-let-7g, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-1-2, bta-mir-1-1, bta-mir-101-1, bta-mir-124a-2, bta-mir-124b, bta-mir-133a-2, bta-mir-133a-1, bta-mir-143, bta-mir-152, bta-mir-154a, bta-mir-185, bta-mir-199a-2, bta-mir-206, bta-mir-30f, bta-mir-335, bta-mir-33a, bta-mir-33b, bta-mir-370, bta-mir-378-1, bta-mir-432, bta-mir-9-1, bta-mir-9-2, bta-mir-1224, bta-mir-376b, bta-mir-376d, bta-mir-376c, bta-mir-376a, bta-mir-1839, bta-mir-1185, bta-mir-2284i, bta-mir-2285a, bta-mir-2284s, bta-mir-2285d, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2285b-1, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-199c, bta-mir-2284p, bta-mir-2284u, bta-mir-2363-1, bta-mir-2363-2, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2285c, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-320b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-2284e, bta-mir-320a-1, bta-mir-378-2, bta-mir-2284w, bta-mir-2284x, bta-mir-3432a-1, bta-mir-3432a-2, bta-mir-3604-1, bta-mir-3604-2, bta-mir-2284y-1, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2284y-2, bta-mir-378b, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2284y-3, bta-mir-154c, bta-mir-376e, bta-mir-154b, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-378c, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-6526-1, bta-mir-6526-2, bta-mir-503, bta-mir-2284y-7, bta-mir-6526-3, bta-mir-2285n-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2285k-2, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2284z-4, bta-mir-2285k-5, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-6536-1, bta-mir-2284aa-4, bta-mir-6536-2, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2284z-2, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2284ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2284ac, bta-mir-2285ae, bta-mir-378d, bta-mir-3432b, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
Such an expression pattern indicates that they may have more dramatic functions, e. g., miR-27 in signaling and immune responses [53], miR-103 in the regulation of neuronal migration by modulating Cyclin -dependent kinase 5, regulatory subunit 1 (CDK5R1) expression [54], and let-7 in developmental timing in Caenorhabditis elegans [55]. [score:8]
For example, miR-103 is a negative regulator of insulin sensitivity by targeting caveolin-1, which is a critical regulator of the insulin receptor [49]. [score:5]
Several miRNAs were found to be highly expressed in particular tissues: miR-1839 in the heart, miR-103 in the liver, miR-9 and -154 in the kidneys, miRNAn25 in fat, and miRNAn26 in muscle and fat tissue, respectively. [score:3]
The expression levels of the 15 miRNAs were determined, and the selected miRNAs included five reported tissue-specific miRNAs (bta-miR-9, miR-124, miR-122, miR-27, and miR-103), seven backfat-predominant miRNAs in fetal and/or adult bovine backfat libraries (let-7a, miR-140, miR-199a, miR-320, miR-2284x, miRn8, and miRn25) as well as three high-read miRNAs (miR-154, miR-1839, and miRn26) in backfat that were compared with the muscle library. [score:2]
Similarly, miR-21, miR-103, miR-107, miR140, miR-143, miR-152, miR-199, miR-432, miR-839, and miR-2284x were predominantly found at lengths of 22, 21, 21, 23, 21, 21, 21, 21, 22, and 22 nt, respectively. [score:1]
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6
[+] score: 16
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-mir-21, hsa-mir-26a-1, hsa-mir-27a, hsa-mir-28, hsa-mir-30a, hsa-mir-96, hsa-mir-98, hsa-mir-99a, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-196a-1, hsa-mir-199a-1, hsa-mir-148a, hsa-mir-30d, hsa-mir-34a, hsa-mir-196a-2, hsa-mir-199a-2, hsa-mir-23b, hsa-mir-27b, hsa-mir-125b-1, hsa-mir-143, hsa-mir-145, hsa-mir-152, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-194-1, hsa-mir-194-2, hsa-mir-200a, hsa-mir-99b, hsa-mir-26a-2, hsa-mir-378a, hsa-mir-342, hsa-mir-148b, hsa-mir-338, hsa-mir-335, hsa-mir-196b, hsa-mir-484, hsa-mir-486-1, hsa-mir-1271, hsa-mir-378d-2, bta-mir-26a-2, bta-mir-103-1, bta-mir-148a, bta-mir-21, bta-mir-27a, bta-mir-30d, bta-mir-484, bta-mir-99a, bta-mir-125a, bta-mir-125b-1, bta-mir-145, bta-mir-199a-1, bta-mir-27b, bta-mir-98, bta-mir-148b, bta-mir-200a, bta-mir-30a, bta-let-7a-1, bta-mir-342, bta-mir-23b, bta-let-7a-2, bta-let-7a-3, bta-mir-125b-2, bta-mir-34a, bta-mir-99b, hsa-mir-885, hsa-mir-103b-1, hsa-mir-103b-2, bta-mir-143, bta-mir-152, bta-mir-16a, bta-mir-194-2, bta-mir-196a-2, bta-mir-196a-1, bta-mir-196b, bta-mir-199a-2, bta-mir-26a-1, bta-mir-28, bta-mir-335, bta-mir-338, bta-mir-378-1, bta-mir-486, bta-mir-885, bta-mir-96, bta-mir-1271, bta-mir-2299, bta-mir-199c, bta-mir-1388, bta-mir-194-1, bta-mir-378-2, hsa-mir-378b, bta-mir-3431, hsa-mir-378c, hsa-mir-4286, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, bta-mir-4286-1, bta-mir-4286-2, hsa-mir-378j, bta-mir-378b, bta-mir-378c, hsa-mir-486-2, bta-mir-378d, bta-mir-194b, bta-mir-194b-2
The expression of miRNA in a sample was normalized to bta-miR-103 and calculated relative to day-14 as follows: ΔCt [target miRNA] = Ct [target miRNA]–Ct [bta-miR-103]; relative expression of target miRNA = 2-ΔΔCt, where ΔΔCt = ΔCt [target miRNA, day+28] - ΔCt [target miRNA, day-14]. [score:13]
In dairy goats, miR-27a and miR-103 have been found to function as regulators of milk fat metabolism and lactation cycle [26, 27]. [score:2]
Bta-miR-103 (product No. [score:1]
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7
[+] score: 16
AA, amino acid; ACACA, Acetyl-coenzyme A carboxylase alpha; ACSL1, Acyl-CoA synthetase long-chain family member 1; ACSS2, Acyl-CoA synthetase short-chain family member 2; AGPAT6, 1-acylglycerol-3-phosphate O-acyltransferase 6; DGAT1, diacylglycerol acyltransferase 1; EAA, essential amino acids; EIF4E, eukaryotic translation initiation factor 4E; EIF4EBP1, eukaryotic translation initiation factor 4E -binding protein 1; FA, fatty acid; FABP3, fatty acid -binding protein, heart; FASN, fatty acid synthase; GADPH, glyceraldehyde 3-phosphate dehydrogenase; INSIG1, insulin induced gene 1; LCFA, long-chain fatty acids; LPIN1, lipin 1; Mac-T, bovine mammary epithelial cells; MEC, mammary epithelial cells; MFD, milk fat-depressing diet; MIR103, microRNA 103; MIR130a, microRNA 130a; MIR21, microRNA 21; MIR27ab, microRNA 27ab; MIR34a, microRNA 34a; MIR378, microRNA 378; MIR448, microRNA 448; miRNA, microRNA; MTOR, mechanistic target of rapamycin (Ser/Thr kinase); mTORC1, mTOR complex 1; NR1H3, liver X receptor α; PPARD, peroxisome proliferator-activated receptor beta; PPARG, peroxisome proliferator-activated receptor gamma; RHEB, ras homolog enriched in brain; RIN, RNA integrity; RMC, rapamycin; RPS6KB1, ribosomal protein S6 kinase beta-1; RPS9, ribosomal protein S9; RXRA, retinoid X receptor, alpha; SCD, stearoyl-CoA desaturase; SREBF1, sterol regulatory element -binding transcription factor 1; TAG, triacylglycerol; TSC1, tuberous sclerosis 1; UXT, ubiquitously expressed prefoldin like chaperone 1: Table S1. [score:10]
In the context of controlling the cellular lipogenic balance, it is noteworthy that PPARG itself alters transcription of MIR103 and MIR378, hence, these miRNA could elicit a pro-lipogenic effect through targets such as FASN [16]. [score:3]
Fig. 2Expression of MIR21, MIR27AB, MIR34A, MIR103, MIR130A, MIR378 and MIR448. [score:3]
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[+] score: 13
miR-103 is up-regulated in goat mammary glands toward mid-lactation and plays an important role in milk fat synthesis in goat mammary epithelial cells [17], suggesting that miRNAs are deeply associated with the differentiation and function of mammary epithelial cells. [score:4]
As well as miR-148a, miR-103 and miR-223 expression in BMEC culture media was also elevated by DIP-treatment, suggesting secretion of miR-103 and miR-223 in BMEC was also enhanced by lactogenic hormones. [score:3]
The medium-to-cell expression ratios of miR-103 (P = 0.025), miR-148a (P < 0.001), and miR-223 (P = 0.013) were elevated in the DIP -treated BMECs, suggesting that the lactogenic differentiation -induced secretion of these three miRNAs in BMECs. [score:3]
The ratios of miR-25, miR-103, miR-148a, and miR-223 were elevated (P = 0.062, = 0.025, < 0.001, and = 0.014, respectively) but those of miR-107, miR-182, and miR-339a were reduced in the DIP -treated BMECs (P = 0.057, = 0.022, and = 0.020, respectively) in comparison to those in the untreated cells (Fig.   4c). [score:1]
The top 20 miRNAs in the milk exosome were let-7b (12.7 %), miR-200c (10.9 %), miR-26a (8.8 %), let-7c (7.5 %), let-7a-5p (6.3 %), miR-30a-5p (3.1 %), miR-320a (2.7 %), miR-103 (2.5 %), miR-107 (2.2 %), let-7d (1.9 %), miR-23-3p (1.6 %), miR-191 (1.6 %), miR-23a (1.6 %), miR-20a (1.5 %), miR-1777b (1.5 %), miR-151-5p (1.4 %), miR-24-3p (1.3 %), miR-320b (1.3 %), miR-200b (1.2 %), and miR-141 (1.2 %) (Fig.   2). [score:1]
Of those, miR-7b, miR-21-5p, miR-23-3p, miR-26a, miR-30a, miR-103, miR-107, miR-148a, miR-200c, and miR-320a were among the top 30 most abundant miRNAs in the milk. [score:1]
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[+] score: 12
For example, in the retroperitoneal adipose tissue of mice, conjugated linoleic acid treatment modified the expression of miR-103, miR-107, miR-143, miR-221 and miR-222, and their expression was correlated with genes that were strongly expressed in adipocytes and related to lipid metabolism [41]. [score:7]
The over -expression of miR-27a or miR-103 in goat mammary epithelial cells led to a change in fat droplet and triglyceride accumulation, lowered the unsaturated/saturated fatty acids ratio and markedly affected the expression of genes associated with lipid metabolism [33, 34]. [score:5]
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[+] score: 11
Other miRNAs from this paper: ssc-mir-122, ssc-mir-125b-2, ssc-mir-181b-2, ssc-mir-20a, ssc-mir-23a, ssc-mir-26a, ssc-mir-29b-1, ssc-mir-181c, ssc-mir-214, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-21, ssc-mir-29c, ssc-mir-30c-2, bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-20a, bta-mir-21, bta-mir-26b, bta-mir-30d, bta-mir-499, bta-mir-99a, bta-mir-125b-1, bta-mir-126, bta-mir-181a-2, bta-mir-199a-1, bta-mir-30b, bta-mir-107, bta-mir-10a, bta-mir-127, bta-mir-142, bta-mir-181b-2, bta-mir-30e, bta-mir-92a-2, bta-let-7d, bta-mir-132, bta-mir-138-2, bta-mir-17, bta-mir-181c, bta-mir-192, bta-mir-199b, bta-mir-200a, bta-mir-200c, bta-mir-214, bta-mir-23a, bta-mir-29b-2, bta-mir-29c, bta-mir-455, bta-let-7g, bta-mir-10b, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-125b-2, bta-mir-99b, ssc-mir-99b, ssc-mir-17, ssc-mir-30b, ssc-mir-199b, bta-mir-1-2, bta-mir-1-1, bta-mir-129-1, bta-mir-129-2, bta-mir-133a-2, bta-mir-133a-1, bta-mir-133b, bta-mir-135b, bta-mir-138-1, bta-mir-143, bta-mir-144, bta-mir-146b, bta-mir-146a, bta-mir-181d, bta-mir-190a, bta-mir-199a-2, bta-mir-202, bta-mir-206, bta-mir-211, bta-mir-212, bta-mir-223, bta-mir-26a-1, bta-mir-29d, bta-mir-30f, bta-mir-338, bta-mir-33a, bta-mir-33b, bta-mir-375, bta-mir-429, bta-mir-451, bta-mir-92a-1, bta-mir-92b, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, bta-mir-181b-1, ssc-mir-133a-1, ssc-mir-1, ssc-mir-146b, ssc-mir-181a-1, ssc-mir-30a, bta-mir-199c, ssc-mir-206, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-133b, ssc-mir-29a, ssc-mir-30d, ssc-mir-30e, ssc-mir-199a-2, ssc-mir-499, ssc-mir-143, ssc-mir-10a, ssc-mir-10b, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-181b-1, ssc-mir-181d, ssc-mir-99a, ssc-mir-92a-2, ssc-mir-92a-1, ssc-mir-92b, ssc-mir-192, ssc-mir-142, ssc-mir-127, ssc-mir-202, ssc-mir-129a, ssc-mir-455, ssc-mir-125b-1, ssc-mir-338, ssc-mir-133a-2, ssc-mir-146a, bta-mir-26c, ssc-mir-30c-1, ssc-mir-126, ssc-mir-199a-1, ssc-mir-451, ssc-let-7a-2, ssc-mir-129b, ssc-mir-429, ssc-let-7d, ssc-let-7f-2, ssc-mir-29b-2, ssc-mir-132, ssc-mir-138, ssc-mir-144, ssc-mir-190a, ssc-mir-212, bta-mir-133c, ssc-mir-26b, ssc-mir-200b, ssc-mir-223, ssc-mir-375, ssc-mir-33b
Some muscle miRNAs have well-defined target genes, as miR-206 regulates IGF-1; miR-1, miR-122, and miR-462 control IGF-2a; the let-7 family regulates MSTN; miR-103 and miR-107 modulate GHR and FSHR; and miR-138 and miR-211 control LHR. [score:5]
For instance, miR-103, miR-107, miR-122, and miR-143 have constrained metabolic functions in vertebrates and can be targeted in future genetic therapies. [score:3]
Among these miRNAs, they predicted conserved targets in fish and humans for miR-103, miR-107, and miR-143 homologs. [score:3]
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[+] score: 10
Out of these miRNAs,miR-15b (up-regulated 2 folds) have been previously reported to show up-regulation using real-time RT-PCR in dose- and time -dependent manner along with other miRNAs (let-7d, miR-16, miR-25, miR-92a, miR-103, miR-107 and miR-451) in the whole blood of C57BL/6 mice injected with LPS [35]. [score:7]
The two miRNAs (bta-miR-103 and -191) were found to highly abundant in all the samples, while bta-miR-21-5p was highly and uniquely expressed in LPS treated PBMCs only. [score:3]
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[+] score: 8
For example, the expression of rumen specific miRNA miR-2299-3p (one of the N efficiency positively associated miRNAs) tended to be lower than that that under RS (P = 0.094) or CS (P = 0.056) diet (Table S7), while the expression of miR-103 in the rumen under RS (P = 0.004) or CS (P = 0.034) diet was higher than that under AL diet (Table S7). [score:5]
For example, compared with AL diet, RS diet regulated AA transport in the rumen by changing expression of ruminal miR-103, -155, -504, -21-3p, and -142-3p (P < 0.05), and AAs phosphorylation through rumen (miR-103, -155, -504, -21-3p, and -142-3p) and liver (miR-497) (Fig. S1). [score:3]
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13
[+] score: 8
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-mir-18a, hsa-mir-21, hsa-mir-23a, hsa-mir-26a-1, hsa-mir-30a, hsa-mir-99a, hsa-mir-103a-2, hsa-mir-103a-1, mmu-mir-1a-1, mmu-mir-23b, mmu-mir-30a, mmu-mir-99a, mmu-mir-126a, mmu-mir-9-2, mmu-mir-133a-1, mmu-mir-138-2, hsa-mir-192, mmu-mir-204, mmu-mir-122, hsa-mir-204, hsa-mir-1-2, hsa-mir-23b, hsa-mir-122, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-138-2, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-126, hsa-mir-138-1, mmu-mir-192, mmu-let-7a-1, mmu-let-7a-2, mmu-mir-18a, mmu-mir-21a, mmu-mir-23a, mmu-mir-26a-1, mmu-mir-103-1, mmu-mir-103-2, hsa-mir-1-1, mmu-mir-1a-2, mmu-mir-26a-2, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-138-1, hsa-mir-26a-2, hsa-mir-376c, hsa-mir-381, mmu-mir-381, mmu-mir-133a-2, rno-let-7a-1, rno-let-7a-2, rno-mir-9a-1, rno-mir-9a-3, rno-mir-9a-2, rno-mir-18a, rno-mir-21, rno-mir-23a, rno-mir-23b, rno-mir-26a, rno-mir-30a, rno-mir-99a, rno-mir-103-2, rno-mir-103-1, rno-mir-122, rno-mir-126a, rno-mir-133a, rno-mir-138-2, rno-mir-138-1, rno-mir-192, rno-mir-204, mmu-mir-411, hsa-mir-451a, mmu-mir-451a, rno-mir-451, hsa-mir-193b, rno-mir-1, mmu-mir-376c, rno-mir-376c, rno-mir-381, hsa-mir-574, hsa-mir-652, hsa-mir-411, bta-mir-26a-2, bta-mir-103-1, bta-mir-16b, bta-mir-18a, bta-mir-21, bta-mir-99a, bta-mir-126, mmu-mir-652, bta-mir-138-2, bta-mir-192, bta-mir-23a, bta-mir-30a, bta-let-7a-1, bta-mir-122, bta-mir-23b, bta-let-7a-2, bta-let-7a-3, bta-mir-204, mmu-mir-193b, mmu-mir-574, rno-mir-411, rno-mir-652, mmu-mir-1b, hsa-mir-103b-1, hsa-mir-103b-2, bta-mir-1-2, bta-mir-1-1, bta-mir-133a-2, bta-mir-133a-1, bta-mir-138-1, bta-mir-193b, bta-mir-26a-1, bta-mir-381, bta-mir-411a, bta-mir-451, bta-mir-9-1, bta-mir-9-2, bta-mir-376c, bta-mir-1388, rno-mir-9b-3, rno-mir-9b-1, rno-mir-126b, rno-mir-9b-2, hsa-mir-451b, bta-mir-574, bta-mir-652, mmu-mir-21b, mmu-mir-21c, mmu-mir-451b, bta-mir-411b, bta-mir-411c, mmu-mir-126b, rno-mir-193b, mmu-mir-9b-2, mmu-mir-9b-1, mmu-mir-9b-3
The qRT-PCR data showed similar expression trend of detected expression of these miRNAs from the library except miR-103. [score:5]
To validate above miRNA expression patterns, quantitative RT-PCR was performed on tissue-specific miRNAs (miR-122, -133a), high cloning frequency miRNAs (miR-26a, -99a and -150) and low cloning frequency miRNAs (miR-103, -107, -411, -423-5p, -574-3p and -652). [score:3]
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14
[+] score: 7
The amplification plot and melting curve was generated during analysis og the expression of miR-654-5p and the two endogenous controls (miR-103 and U6) in granulosa cells co-cultured with exosomes derived from follicular fluid containing growing (BCB-) oocytes. [score:3]
0078505.g003 Figure 3 The amplification plot and melting curve was generated during analysis og the expression of miR-654-5p and the two endogenous controls (miR-103 and U6) in granulosa cells co-cultured with exosomes derived from follicular fluid containing growing (BCB-) oocytes. [score:3]
Representative amplification plot (A) and melting curve (B) of miR-654-5p, miR-103 and U6. [score:1]
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15
[+] score: 7
All other miRNAs tested (miR-15a, miR-23b-3p, miR-27b, miR-30d-5p, miR-92a, miR-140, miR-197, miR-345-5p, miR-451, miR-885, miR-2284x, miR-2295, miR-2412, and miR-2478) were not differently expressed between the groups (P > 0.10), except that miR-103 content tended to be higher in the grain-fed cattle than in the grazing cattle (P = 0.057). [score:3]
[1]log2(G/H): the value of log2(grazing/grain-fed)We then conducted qPCR for the contents of c-miRNAs of interest, not only to validate the results of microarray analysis for those 13 miRNAs and the miRNAs below the top 20 in SAM analysis (miR-29b, miR-197), but also to further explore potential feeding -induced miRNAs among adipose-enriched (miR-15a, miR-19a, miR-27b, miR-92a, miR-103, miR-142-5p), plasma-enriched (miR-2412, miR-2478), and bovine-specific (miR-2284x and miR-2295) miRNAs [31]. [score:1]
In contrast, the plasma samples were enriched with moderate concentrations of miRNAs associated with adipose tissue, such as miR-15a, miR-19a/b, miR-21, miR-27b, miR-92a/b, miR-103, miR-106a/b, miR-107, miR-125b, and miR-150 [11, 12]. [score:1]
We also conducted qPCR of miRNAs that are enriched in muscle or plasma (miR-21-5p, miR-30d-5p, miR-103, miR-206, miR-208b, miR-451, miR-486, miR-499, miR-2412, and miR-2478), some of which are abundant in bovine skeletal muscles [4]. [score:1]
[1]log2(G/H): the value of log2(grazing/grain-fed) We then conducted qPCR for the contents of c-miRNAs of interest, not only to validate the results of microarray analysis for those 13 miRNAs and the miRNAs below the top 20 in SAM analysis (miR-29b, miR-197), but also to further explore potential feeding -induced miRNAs among adipose-enriched (miR-15a, miR-19a, miR-27b, miR-92a, miR-103, miR-142-5p), plasma-enriched (miR-2412, miR-2478), and bovine-specific (miR-2284x and miR-2295) miRNAs [31]. [score:1]
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16
[+] score: 6
miR-103 and miR-107 were found to be up-regulated in adipocyte differentiation [51] and have been reported to play a role in the regulation of insulin sensitivity [52]. [score:5]
Also, some miRNAs are inside introns of more than one gene as is the case for miR-103 within the introns of pantothenate kinase 2 (PANK2) and pantothenate kinase 3 (PANK3). [score:1]
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[+] score: 3
In humans, high levels of some miRNAs (i. e., hsa-miR-29a and hsa-miR-103) appear to be associated with poor gestational prognosis. [score:1]
In contrast with this finding, we detected different abundance patterns of bta-miR-29a and bta-miR-103 in our samples; these miRNAs were highly abundance levels in the groups that reached term (C-LTP and AI-LTP). [score:1]
non-pregnant cows reported by Ioannidis and Dounadue (2016), only seven (bta-let-7d, bta-let-7f, bta-miR-101, bta-miR-103, bta-miR-29a, bta-miR-29c and bta-miR374b) were found to have different abundance levels among the groups in our study. [score:1]
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[+] score: 3
Other miRNAs from this paper: ssc-mir-122, ssc-mir-135-1, ssc-mir-135-2, ssc-mir-148a, ssc-mir-19a, ssc-mir-20a, ssc-mir-224, ssc-mir-24-1, ssc-mir-323, ssc-mir-140, ssc-mir-183, ssc-mir-214, ssc-mir-27a, ssc-mir-325, ssc-let-7c, ssc-let-7f-1, ssc-let-7i, ssc-mir-103-1, ssc-mir-107, ssc-mir-136, ssc-mir-153, ssc-mir-18a, ssc-mir-186, ssc-mir-196a-2, ssc-mir-204, ssc-mir-21, bta-mir-18b, bta-let-7f-2, bta-mir-101-2, bta-mir-103-1, bta-mir-148a, bta-mir-18a, bta-mir-20a, bta-mir-21, bta-mir-221, bta-mir-27a, bta-mir-27b, bta-mir-107, bta-mir-140, bta-mir-20b, bta-mir-215, bta-let-7d, bta-mir-17, bta-mir-186, bta-mir-199b, bta-mir-210, bta-mir-214, bta-mir-450a-2, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-let-7f-1, bta-mir-122, bta-let-7i, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-15a, bta-mir-19a, bta-mir-204, ssc-mir-15a, ssc-mir-17, ssc-mir-199b, ssc-mir-210, ssc-mir-221, bta-mir-101-1, bta-mir-133a-2, bta-mir-133a-1, bta-mir-135a-2, bta-mir-135a-1, bta-mir-135b, bta-mir-136, bta-mir-146b, bta-mir-153-1, bta-mir-153-2, bta-mir-183, bta-mir-24-1, bta-mir-196a-2, bta-mir-196a-1, bta-mir-196b, bta-mir-224, bta-mir-323, ssc-mir-101-1, ssc-mir-101-2, ssc-mir-133a-1, ssc-mir-450a, ssc-mir-146b, ssc-mir-215, bta-mir-1343, bta-mir-2320, bta-mir-2326, bta-mir-2366, bta-mir-2411, bta-mir-2483, bta-mir-450a-1, ssc-let-7a-1, ssc-let-7e, ssc-let-7g, ssc-mir-103-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-196b-1, ssc-mir-450b, ssc-mir-450c, ssc-mir-133a-2, ssc-let-7a-2, ssc-mir-18b, ssc-mir-1343, ssc-mir-2320, bta-mir-450b, ssc-let-7d, ssc-let-7f-2, ssc-mir-20b-1, ssc-mir-20b-2, ssc-mir-196a-1, ssc-mir-196b-2, ssc-mir-2366-1, ssc-mir-2366-2, ssc-mir-2411, ssc-mir-2483
For example, ssc-miR-153, miR-325, miR-135-1*, miR-135-2*, miR-146b*, miR-15a*, miR-215* and miR-323* were only identified in Large White, contrary to the patterns displayed by ssc-miR-101a-1*, miR-103*, miR-183*, miR-1a* and miR-210*, indicating that these miRNAs may function in the physiology or development of the backfat tissue. [score:2]
Several miRNAs such as let-7, miR-27 and miR-103 are reported to perform very important functions in adipogenesis [33]– [35]. [score:1]
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[+] score: 3
Variant expression followed two main patterns as seen in other studies [40] those miRNAs with a strong predominant isomiR, such as bta-miR-191 and bta-miR-103; and those miRNAs where there is no predominant isomiR, such as bta-miR-486 and bta-miR-320a. [score:3]
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[+] score: 3
Other miRNAs from this paper: bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-151, bta-mir-30d, bta-mir-320a-2, bta-mir-126, bta-mir-181a-2, bta-mir-27b, bta-mir-30b, bta-mir-31, bta-mir-34b, bta-mir-107, bta-mir-15b, bta-mir-181b-2, bta-mir-30e, bta-let-7d, bta-mir-124a-1, bta-mir-138-2, bta-mir-181c, bta-mir-214, bta-mir-455, bta-mir-93, bta-let-7g, bta-mir-10b, bta-mir-30a, bta-let-7a-1, bta-mir-487b, bta-let-7f-1, bta-mir-122, bta-mir-30c, bta-let-7i, bta-mir-25, bta-mir-34c, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-15a, bta-mir-34a, bta-mir-1-2, bta-mir-1-1, bta-mir-105b, bta-mir-124a-2, bta-mir-124b, bta-mir-133a-2, bta-mir-133a-1, bta-mir-133b, bta-mir-138-1, bta-mir-152, bta-mir-181d, bta-mir-196a-2, bta-mir-196a-1, bta-mir-206, bta-mir-30f, bta-mir-409a, bta-mir-432, bta-mir-486, bta-mir-495, bta-mir-543, bta-mir-9-1, bta-mir-9-2, bta-mir-1185, bta-mir-1271, bta-mir-181a-1, bta-mir-181b-1, bta-mir-2284i, bta-mir-2284s, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2284p, bta-mir-2284u, bta-mir-2363-1, bta-mir-2363-2, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2384, bta-mir-2284v, bta-mir-2284q, bta-mir-2404-1, bta-mir-2284m, bta-mir-2284b, bta-mir-320b, bta-mir-2424, bta-mir-2284r, bta-mir-2284h, bta-mir-2404-2, bta-mir-2284o, bta-mir-2284e, bta-mir-320a-1, bta-mir-424, bta-mir-2284w, bta-mir-2284x, bta-mir-409b, bta-mir-2284y-1, bta-mir-2284y-2, bta-mir-2284y-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2284y-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2284z-4, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2284z-2, bta-mir-133c, bta-mir-2284ab, bta-mir-2284ac
For example, the expression of miR-103, miR-107, and miR-25 was higher in fetal bovine muscle tissue, in contrast with the patterns shown by miR-1, miR-133a, and miR-29a in adult bovine muscle tissue. [score:3]
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[+] score: 3
In addition, miR-191 and miR-103 have been used as endogenous reference miRNAs in human tissues [8]; however, in our study, miR-191 failed to meet the endogenous normalizer criteria, and miR-103 was expressed at a low level in bovine serum. [score:3]
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[+] score: 3
The relative expression in HM and LM fractions of several of these knowns, including bta-miR-103, bta-miR-30b-5p, bta-miR-17-5p, bta-miR-106b, bta-miR-142-3p, bta-miR-34b, bta-miR-18a, bta-miR-34c, bta-miR-455-5p, bta-miR-10b, bta-miR-99b, bta-miR-1246, bta-miR-99a-5p, and bta-miR-1388-5p, was consistent with the relative abundance of their homologouss, observed in the normal vs abnormal sperm. [score:3]
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[+] score: 3
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-26a-1, hsa-mir-26b, hsa-mir-27a, hsa-mir-29a, hsa-mir-32, hsa-mir-33a, hsa-mir-99a, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-107, mmu-let-7g, mmu-let-7i, mmu-mir-15b, mmu-mir-99a, mmu-mir-126a, mmu-mir-128-1, mmu-mir-130a, mmu-mir-140, mmu-mir-154, mmu-mir-204, mmu-mir-143, hsa-mir-204, hsa-mir-211, hsa-mir-218-1, hsa-mir-218-2, hsa-mir-222, hsa-mir-223, mmu-mir-301a, mmu-mir-34c, mmu-mir-34b, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-15b, hsa-mir-128-1, hsa-mir-130a, hsa-mir-140, hsa-mir-143, hsa-mir-126, hsa-mir-129-2, hsa-mir-154, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-26a-1, mmu-mir-26b, mmu-mir-29a, mmu-mir-29c, mmu-mir-27a, mmu-mir-129-2, mmu-mir-103-1, mmu-mir-103-2, mmu-mir-340, mmu-mir-107, mmu-mir-32, mmu-mir-218-1, mmu-mir-218-2, mmu-mir-223, mmu-mir-26a-2, mmu-mir-211, mmu-mir-222, mmu-mir-128-2, hsa-mir-128-2, hsa-mir-29c, hsa-mir-101-2, hsa-mir-34b, hsa-mir-34c, hsa-mir-301a, hsa-mir-26a-2, hsa-mir-379, mmu-mir-379, hsa-mir-340, mmu-mir-409, hsa-mir-409, hsa-mir-499a, hsa-mir-455, hsa-mir-670, mmu-mir-1249, mmu-mir-670, mmu-mir-499, mmu-mir-455, bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-101-2, bta-mir-103-1, bta-mir-16b, bta-mir-222, bta-mir-26b, bta-mir-27a, bta-mir-499, bta-mir-99a, bta-mir-126, bta-mir-128-1, bta-mir-34b, bta-mir-107, bta-mir-140, bta-mir-15b, bta-mir-218-2, bta-let-7d, bta-mir-29c, bta-mir-455, bta-let-7g, bta-let-7a-1, bta-let-7f-1, bta-let-7i, bta-mir-34c, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-204, hsa-mir-1249, hsa-mir-1306, hsa-mir-103b-1, hsa-mir-103b-2, bta-mir-128-2, bta-mir-129-2, bta-mir-130a, bta-mir-143, bta-mir-154a, bta-mir-211, bta-mir-218-1, bta-mir-223, bta-mir-26a-1, bta-mir-301a, bta-mir-32, bta-mir-33a, bta-mir-340, bta-mir-379, bta-mir-409a, bta-mir-670, mmu-mir-1306, bta-mir-1306, bta-mir-1249, bta-mir-2284i, bta-mir-2285a, bta-mir-2284s, bta-mir-2285d, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2285b-1, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2284p, bta-mir-2284u, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2285c, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-2284e, hsa-mir-1260b, bta-mir-2284w, bta-mir-2284x, bta-mir-409b, hsa-mir-499b, bta-mir-1260b, bta-mir-2284y-1, bta-mir-2285e-1, bta-mir-2285e-2, bta-mir-2285f-1, bta-mir-2285f-2, bta-mir-2285g-1, bta-mir-2285h, bta-mir-2285i, bta-mir-2285j-1, bta-mir-2285j-2, bta-mir-2285k-1, bta-mir-2285l, bta-mir-6119, mmu-let-7j, bta-mir-2285o-1, bta-mir-2285o-2, bta-mir-2285n-1, bta-mir-2285n-2, bta-mir-2285p, bta-mir-2285m-1, bta-mir-2285m-2, bta-mir-2284y-2, bta-mir-2285n-3, bta-mir-2285n-4, bta-mir-2284y-3, bta-mir-154c, bta-mir-154b, bta-mir-2285o-3, bta-mir-2285o-4, bta-mir-2285m-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2285m-4, bta-mir-2285o-5, bta-mir-2285m-5, bta-mir-2285n-5, bta-mir-2285n-6, bta-mir-2284y-7, bta-mir-2285n-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2285k-2, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2285k-3, bta-mir-2285k-4, bta-mir-2284z-4, bta-mir-2285k-5, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2285q, bta-mir-2285r, bta-mir-2285s, bta-mir-2285t, bta-mir-2285b-2, bta-mir-2285v, bta-mir-2284z-2, mmu-let-7k, mmu-mir-126b, bta-mir-2285g-2, bta-mir-2285g-3, bta-mir-2285af-1, bta-mir-2285af-2, bta-mir-2285y, bta-mir-2285w, bta-mir-2285x, bta-mir-2285z, bta-mir-2285u, bta-mir-2285aa, bta-mir-2285ab, bta-mir-2284ab, bta-mir-2285ac, bta-mir-2285ad, bta-mir-2284ac, bta-mir-2285ae, chi-let-7a, chi-let-7b, chi-let-7c, chi-let-7d, chi-let-7e, chi-let-7f, chi-let-7g, chi-let-7i, chi-mir-103, chi-mir-107, chi-mir-1249, chi-mir-126, chi-mir-1306, chi-mir-130a, chi-mir-140, chi-mir-143, chi-mir-154a, chi-mir-154b, chi-mir-15b, chi-mir-16b, chi-mir-204, chi-mir-211, chi-mir-222, chi-mir-223, chi-mir-2284a, chi-mir-2284b, chi-mir-2284c, chi-mir-2284d, chi-mir-2284e, chi-mir-26a, chi-mir-26b, chi-mir-27a, chi-mir-29a, chi-mir-29c, chi-mir-301a, chi-mir-33a, chi-mir-340, chi-mir-34b, chi-mir-34c, chi-mir-379, chi-mir-409, chi-mir-455, chi-mir-499, chi-mir-99a, bta-mir-2285ag, bta-mir-2285ah, bta-mir-2285ai, bta-mir-2285aj, bta-mir-2285ak, bta-mir-2285al, bta-mir-2285am, bta-mir-2285ar, bta-mir-2285as-1, bta-mir-2285as-2, bta-mir-2285as-3, bta-mir-2285at-1, bta-mir-2285at-2, bta-mir-2285at-3, bta-mir-2285at-4, bta-mir-2285au, bta-mir-2285av, bta-mir-2285aw, bta-mir-2285ax-1, bta-mir-2285ax-2, bta-mir-2285ax-3, bta-mir-2285ay, bta-mir-2285az, bta-mir-2285an, bta-mir-2285ao-1, bta-mir-2285ao-2, bta-mir-2285ap, bta-mir-2285ao-3, bta-mir-2285aq-1, bta-mir-2285aq-2, bta-mir-2285ba-1, bta-mir-2285ba-2, bta-mir-2285bb, bta-mir-2285bc, bta-mir-2285bd, bta-mir-2285be, bta-mir-2285bf-1, bta-mir-2285bf-2, bta-mir-2285bf-3, bta-mir-2285bg, bta-mir-2285bh, bta-mir-2285bi-1, bta-mir-2285bi-2, bta-mir-2285bj-1, bta-mir-2285bj-2, bta-mir-2285bk, bta-mir-2285bl, bta-mir-2285bm, bta-mir-2285bn, bta-mir-2285bo, bta-mir-2285bp, bta-mir-2285bq, bta-mir-2285br, bta-mir-2285bs, bta-mir-2285bt, bta-mir-2285bu-1, bta-mir-2285bu-2, bta-mir-2285bv, bta-mir-2285bw, bta-mir-2285bx, bta-mir-2285by, bta-mir-2285bz, bta-mir-2285ca, bta-mir-2285cb, bta-mir-2285cc, bta-mir-2285cd, bta-mir-2285ce, bta-mir-2285cf, bta-mir-2285cg, bta-mir-2285ch, bta-mir-2285ci, bta-mir-2285cj, bta-mir-2285ck, bta-mir-2285cl, bta-mir-2285cm, bta-mir-2285cn, bta-mir-2285co, bta-mir-2285cp, bta-mir-2285cq, bta-mir-2285cr-1, bta-mir-2285cr-2, bta-mir-2285cs, bta-mir-2285ct, bta-mir-2285cu, bta-mir-2285cv-1, bta-mir-2285cv-2, bta-mir-2285cw-1, bta-mir-2285cw-2, bta-mir-2285cx, bta-mir-2285cy, bta-mir-2285cz, bta-mir-2285da, bta-mir-2285db, bta-mir-2285dc, bta-mir-2285dd, bta-mir-2285de, bta-mir-2285df, bta-mir-2285dg, bta-mir-2285dh, bta-mir-2285di, bta-mir-2285dj, bta-mir-2285dk, bta-mir-2285dl-1, bta-mir-2285dl-2, bta-mir-2285dm
In the goat, a potential role for miR-103 and miR-27a in the regulation of milk fat synthesis in mammary epithelial cells has been reported [34, 35]. [score:2]
Among these, 16 precursors were found amongst the 43 conserved between human, mouse, cow and goat in our analysis (let-7 g, mir-101-2, mir-103, mir-107, mir-128-1, mir-1306, mir-140, mir-15b, mir-16b, mir-211, mir-218-1, mir-26a-1, mir-32, mir-33a, mir-455, let-7-2), so the location of these precursors appears to be highly conserved in all vertebrates. [score:1]
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[+] score: 3
For example, the highly expressed region of the mature bta-miR-103 in the current study is 5 bases shorter than the reported bovine miRNA in miRBase v21 (Fig. 4B). [score:3]
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[+] score: 3
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-16-1, hsa-mir-20a, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-27a, hsa-mir-31, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-101-1, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-16-2, hsa-mir-192, hsa-mir-199a-1, hsa-mir-30c-2, hsa-mir-199a-2, hsa-mir-223, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-125b-1, hsa-mir-132, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-140, hsa-mir-141, hsa-mir-152, hsa-mir-191, hsa-mir-125a, hsa-mir-125b-2, hsa-mir-149, hsa-mir-150, hsa-mir-320a, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-101-2, hsa-mir-99b, hsa-mir-26a-2, hsa-mir-379, hsa-mir-423, hsa-mir-451a, hsa-mir-486-1, hsa-mir-496, hsa-mir-520a, hsa-mir-525, hsa-mir-518b, hsa-mir-516b-2, hsa-mir-516b-1, hsa-mir-516a-1, hsa-mir-516a-2, hsa-mir-92b, hsa-mir-320b-1, hsa-mir-320c-1, hsa-mir-320b-2, bta-mir-26a-2, bta-let-7f-2, bta-mir-101-2, bta-mir-103-1, bta-mir-16b, bta-mir-20a, bta-mir-21, bta-mir-27a, bta-mir-320a-2, bta-mir-125a, bta-mir-125b-1, bta-mir-199a-1, bta-mir-31, bta-mir-140, bta-mir-92a-2, bta-let-7d, bta-mir-132, bta-mir-191, bta-mir-192, bta-mir-22, bta-mir-23a, bta-mir-29c, bta-mir-423, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-mir-150, bta-let-7f-1, bta-mir-30c, bta-let-7i, bta-mir-23b, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-125b-2, bta-mir-99b, hsa-mir-1249, hsa-mir-103b-1, hsa-mir-103b-2, hsa-mir-320d-1, hsa-mir-320c-2, hsa-mir-320d-2, bta-mir-101-1, bta-mir-133a-2, bta-mir-133a-1, bta-mir-141, bta-mir-152, bta-mir-16a, bta-mir-24-1, bta-mir-199a-2, bta-mir-223, bta-mir-26a-1, bta-mir-379, bta-mir-451, bta-mir-486, bta-mir-496, bta-mir-92a-1, bta-mir-92b, bta-mir-1249, bta-mir-320b, bta-mir-320a-1, hsa-mir-320e, hsa-mir-23c, hsa-mir-451b, bta-mir-149, hsa-mir-486-2
Thus, only four of all miRNAs differentially expressed (P < 0.05) between pregnant (Day 24) and non-pregnant animals in the PCR array, i. e. miR-99b, miR-152, miR-101, miR-103, were also different (P < 0.05) between pregnant and non-pregnant groups (all comparisons) in the sequencing dataset. [score:3]
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26
[+] score: 2
Other miRNAs from this paper: bta-mir-103-1, bta-mir-27b, bta-mir-2332, bta-mir-1434
Among the top 10 highly conserved miRNAs (Figure 5), miR-103/107 [51, 52] and miR-27b/27a-3p [53, 54] families are well known for their roles in the regulation of adipogenesis, a function that may be universal in most vertebrates. [score:2]
[1 to 20 of 1 sentences]
27
[+] score: 1
Although circulation levels of miR-29b, miR-30a, miR-30d, miR-103, miR-126-5p, miR-144, miR-155, miR-425-5p, miR-489, miR-1249, and miR-2888 were also examined, no significant differences in these miRNAs were observed between the cattle groups at any time points or between the time points in either of the cattle groups. [score:1]
[1 to 20 of 1 sentences]
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[+] score: 1
The geometric mean of bta-miR-103 and bta-miR-25 was used as endogenous control. [score:1]
[1 to 20 of 1 sentences]
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[+] score: 1
Moreover, miR-103 [32], miR-2885, miR-135a and miRNA-370 are involved in metabolism of glucose and lipids 33– 35. [score:1]
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[+] score: 1
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-21, hsa-mir-23a, hsa-mir-30a, hsa-mir-92a-1, hsa-mir-92a-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-196a-1, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-30d, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-196a-2, hsa-mir-210, hsa-mir-181a-1, hsa-mir-218-1, hsa-let-7g, hsa-let-7i, hsa-mir-23b, hsa-mir-30b, hsa-mir-128-1, hsa-mir-145, hsa-mir-191, hsa-mir-181b-2, hsa-mir-128-2, hsa-mir-30c-1, hsa-mir-99b, hsa-mir-296, hsa-mir-30e, hsa-mir-361, hsa-mir-337, hsa-mir-148b, hsa-mir-196b, hsa-mir-425, hsa-mir-20b, hsa-mir-486-1, hsa-mir-488, hsa-mir-181d, hsa-mir-498, hsa-mir-519c, hsa-mir-520a, hsa-mir-526b, hsa-mir-520d, hsa-mir-506, hsa-mir-92b, hsa-mir-608, hsa-mir-617, hsa-mir-625, hsa-mir-641, hsa-mir-1264, hsa-mir-1271, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-21, bta-mir-30d, bta-mir-128-1, bta-mir-145, bta-mir-181a-2, bta-mir-30b, bta-mir-181b-2, bta-mir-20b, bta-mir-30e, bta-mir-92a-2, bta-let-7d, bta-mir-148b, bta-mir-181c, bta-mir-191, bta-mir-210, bta-mir-23a, bta-mir-361, bta-mir-425, bta-let-7g, bta-mir-30a, bta-let-7a-1, bta-let-7f-1, bta-mir-30c, bta-let-7i, bta-mir-23b, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-99b, hsa-mir-890, hsa-mir-888, hsa-mir-889, hsa-mir-938, hsa-mir-1184-1, hsa-mir-1203, hsa-mir-1204, hsa-mir-1265, hsa-mir-103b-1, hsa-mir-103b-2, bta-mir-128-2, bta-mir-181d, bta-mir-196a-2, bta-mir-196a-1, bta-mir-196b, bta-mir-218-1, bta-mir-296, bta-mir-30f, bta-mir-486, bta-mir-488, bta-mir-92a-1, bta-mir-92b, bta-mir-1271, bta-mir-181a-1, bta-mir-181b-1, bta-mir-148c, hsa-mir-1184-2, hsa-mir-1184-3, hsa-mir-486-2, bta-mir-1264, bta-mir-148d
php) and normalization was performed using the geometric mean of miR-23a, miR-103, miR-191 and SNORD49A. [score:1]
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[+] score: 1
These miRNAs include: miR-33 [7], miR-122 [8], miR-370 [9], miR-378/378* [10], miR-27 [11], miR-143 [12], miR-335 [13], miR-103 [14] and so on. [score:1]
[1 to 20 of 1 sentences]
32
[+] score: 1
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-16-1, hsa-mir-21, hsa-mir-22, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-27a, hsa-mir-31, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-16-2, hsa-mir-192, hsa-mir-148a, hsa-mir-30c-2, hsa-mir-181a-2, hsa-mir-205, hsa-mir-181a-1, hsa-mir-214, hsa-mir-219a-1, hsa-mir-221, hsa-mir-222, hsa-mir-223, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-30b, hsa-mir-125b-1, hsa-mir-191, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, hsa-mir-146a, hsa-mir-184, hsa-mir-186, hsa-mir-193a, hsa-mir-194-1, hsa-mir-155, hsa-mir-194-2, hsa-mir-29c, hsa-mir-30c-1, hsa-mir-200a, hsa-mir-219a-2, hsa-mir-99b, hsa-mir-26a-2, hsa-mir-365a, hsa-mir-365b, hsa-mir-374a, hsa-mir-148b, hsa-mir-423, hsa-mir-486-1, hsa-mir-499a, hsa-mir-532, hsa-mir-590, bta-mir-26a-2, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-16b, bta-mir-21, bta-mir-221, bta-mir-222, bta-mir-27a, bta-mir-499, bta-mir-125b-1, bta-mir-181a-2, bta-mir-205, bta-mir-27b, bta-mir-30b, bta-mir-31, bta-mir-193a, bta-let-7d, bta-mir-148b, bta-mir-186, bta-mir-191, bta-mir-192, bta-mir-200a, bta-mir-214, bta-mir-22, bta-mir-23a, bta-mir-29c, bta-mir-423, bta-let-7g, bta-mir-24-2, bta-let-7a-1, bta-mir-532, bta-let-7f-1, bta-mir-30c, bta-let-7i, bta-let-7a-2, bta-let-7a-3, bta-let-7b, bta-let-7c, bta-let-7e, bta-mir-125b-2, bta-mir-365-1, bta-mir-374a, bta-mir-99b, hsa-mir-374b, hsa-mir-664a, hsa-mir-103b-1, hsa-mir-103b-2, hsa-mir-1915, bta-mir-146a, bta-mir-155, bta-mir-16a, bta-mir-184, bta-mir-24-1, bta-mir-194-2, bta-mir-219-1, bta-mir-223, bta-mir-26a-1, bta-mir-365-2, bta-mir-374b, bta-mir-486, bta-mir-763, bta-mir-9-1, bta-mir-9-2, bta-mir-181a-1, bta-mir-2284i, bta-mir-2284s, bta-mir-2284l, bta-mir-2284j, bta-mir-2284t, bta-mir-2284d, bta-mir-2284n, bta-mir-2284g, bta-mir-2339, bta-mir-2284p, bta-mir-2284u, bta-mir-2284f, bta-mir-2284a, bta-mir-2284k, bta-mir-2284c, bta-mir-2284v, bta-mir-2284q, bta-mir-2284m, bta-mir-2284b, bta-mir-2284r, bta-mir-2284h, bta-mir-2284o, bta-mir-664a, bta-mir-2284e, bta-mir-1388, bta-mir-194-1, bta-mir-193a-2, bta-mir-2284w, bta-mir-2284x, bta-mir-148c, hsa-mir-374c, hsa-mir-219b, hsa-mir-499b, hsa-mir-664b, bta-mir-2284y-1, bta-mir-2284y-2, bta-mir-2284y-3, bta-mir-2284y-4, bta-mir-2284y-5, bta-mir-2284y-6, bta-mir-2284y-7, bta-mir-2284z-1, bta-mir-2284aa-1, bta-mir-2284z-3, bta-mir-2284aa-2, bta-mir-2284aa-3, bta-mir-2284z-4, bta-mir-2284z-5, bta-mir-2284z-6, bta-mir-2284z-7, bta-mir-2284aa-4, bta-mir-2284z-2, hsa-mir-486-2, hsa-mir-6516, bta-mir-2284ab, bta-mir-664b, bta-mir-6516, bta-mir-219-2, bta-mir-2284ac, bta-mir-219b, bta-mir-374c, bta-mir-148d
Here we added a symbol “`” after miRNA to represent the antisense of a known miRNA precursor (Table  2), i. e. hsa-mir-103 and mir-103′instead of mir-103a, b, respectively. [score:1]
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[+] score: 1
The geometric mean of miR-103 and miR-25 was used as endogenous control. [score:1]
[1 to 20 of 1 sentences]
34
[+] score: 1
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-15a, hsa-mir-16-1, hsa-mir-21, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26a-1, hsa-mir-29a, hsa-mir-30a, hsa-mir-31, hsa-mir-99a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-16-2, hsa-mir-192, hsa-mir-148a, hsa-mir-10b, hsa-mir-181a-2, hsa-mir-181a-1, hsa-mir-215, hsa-mir-223, hsa-mir-224, hsa-mir-200b, hsa-mir-15b, hsa-mir-27b, hsa-mir-125b-1, hsa-mir-141, hsa-mir-143, hsa-mir-152, hsa-mir-125b-2, hsa-mir-126, hsa-mir-146a, hsa-mir-184, hsa-mir-200c, hsa-mir-155, hsa-mir-29c, hsa-mir-200a, hsa-mir-99b, hsa-mir-296, hsa-mir-30e, hsa-mir-26a-2, hsa-mir-378a, hsa-mir-342, hsa-mir-148b, hsa-mir-451a, ssc-mir-125b-2, ssc-mir-148a, ssc-mir-15b, ssc-mir-184, ssc-mir-224, ssc-mir-23a, ssc-mir-24-1, ssc-mir-26a, ssc-mir-29b-1, ssc-let-7f-1, ssc-mir-103-1, ssc-mir-21, ssc-mir-29c, hsa-mir-486-1, hsa-mir-499a, hsa-mir-671, hsa-mir-378d-2, bta-mir-26a-2, bta-mir-29a, bta-let-7f-2, bta-mir-103-1, bta-mir-148a, bta-mir-16b, bta-mir-21, bta-mir-499, bta-mir-99a, bta-mir-125b-1, bta-mir-126, bta-mir-181a-2, bta-mir-27b, bta-mir-31, bta-mir-15b, bta-mir-215, bta-mir-30e, bta-mir-148b, bta-mir-192, bta-mir-200a, bta-mir-200c, bta-mir-23a, bta-mir-29b-2, bta-mir-29c, bta-mir-10b, bta-mir-24-2, bta-mir-30a, bta-mir-200b, bta-let-7a-1, bta-mir-342, bta-let-7f-1, bta-let-7a-2, bta-let-7a-3, bta-mir-125b-2, bta-mir-15a, bta-mir-99b, hsa-mir-664a, ssc-mir-99b, hsa-mir-103b-1, hsa-mir-103b-2, ssc-mir-15a, ssc-mir-16-2, ssc-mir-16-1, bta-mir-141, bta-mir-143, bta-mir-146a, bta-mir-152, bta-mir-155, bta-mir-16a, bta-mir-184, bta-mir-24-1, bta-mir-223, bta-mir-224, bta-mir-26a-1, bta-mir-296, bta-mir-29d, bta-mir-378-1, bta-mir-451, bta-mir-486, bta-mir-671, bta-mir-29e, bta-mir-29b-1, bta-mir-181a-1, ssc-mir-181a-1, ssc-mir-215, ssc-mir-30a, bta-mir-2318, bta-mir-2339, bta-mir-2430, bta-mir-664a, bta-mir-378-2, ssc-let-7a-1, ssc-mir-378-1, ssc-mir-29a, ssc-mir-30e, ssc-mir-499, ssc-mir-143, ssc-mir-10b, ssc-mir-486-1, ssc-mir-152, ssc-mir-103-2, ssc-mir-181a-2, ssc-mir-27b, ssc-mir-24-2, ssc-mir-99a, ssc-mir-148b, ssc-mir-664, ssc-mir-192, ssc-mir-342, ssc-mir-125b-1, oar-mir-21, oar-mir-29a, oar-mir-125b, oar-mir-181a-1, hsa-mir-378b, hsa-mir-378c, ssc-mir-296, ssc-mir-155, ssc-mir-146a, bta-mir-148c, ssc-mir-126, ssc-mir-378-2, ssc-mir-451, hsa-mir-378d-1, hsa-mir-378e, hsa-mir-378f, hsa-mir-378g, hsa-mir-378h, hsa-mir-378i, hsa-mir-451b, hsa-mir-499b, ssc-let-7a-2, ssc-mir-486-2, hsa-mir-664b, hsa-mir-378j, ssc-let-7f-2, ssc-mir-29b-2, ssc-mir-31, ssc-mir-671, bta-mir-378b, bta-mir-378c, hsa-mir-486-2, oar-let-7a, oar-let-7f, oar-mir-103, oar-mir-10b, oar-mir-143, oar-mir-148a, oar-mir-152, oar-mir-16b, oar-mir-181a-2, oar-mir-200a, oar-mir-200b, oar-mir-200c, oar-mir-23a, oar-mir-26a, oar-mir-29b-1, oar-mir-30a, oar-mir-99a, bta-mir-664b, chi-let-7a, chi-let-7f, chi-mir-103, chi-mir-10b, chi-mir-125b, chi-mir-126, chi-mir-141, chi-mir-143, chi-mir-146a, chi-mir-148a, chi-mir-148b, chi-mir-155, chi-mir-15a, chi-mir-15b, chi-mir-16a, chi-mir-16b, chi-mir-184, chi-mir-192, chi-mir-200a, chi-mir-200b, chi-mir-200c, chi-mir-215, chi-mir-21, chi-mir-223, chi-mir-224, chi-mir-2318, chi-mir-23a, chi-mir-24, chi-mir-26a, chi-mir-27b, chi-mir-296, chi-mir-29a, chi-mir-29b, chi-mir-29c, chi-mir-30a, chi-mir-30e, chi-mir-342, chi-mir-378, chi-mir-451, chi-mir-499, chi-mir-671, chi-mir-99a, chi-mir-99b, bta-mir-378d, ssc-mir-378b, oar-mir-29b-2, ssc-mir-141, ssc-mir-200b, ssc-mir-223, bta-mir-148d
Another miRNA, miR-103, was shown to control milk fat accummulation in goat mammary gland during lactation (Lin et al., 2013). [score:1]
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