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4 publications mentioning dme-mir-982

Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-982. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 27
The expression levels of miR-252–5p and miR-982–5p change post-transcriptionally following Dis3 knockdown. [score:4]
For the downregulated miRNA, miR-982–5p, our data are consistent with a role of Dis3 in the processing of the miRNA from the pre-miRNA precursor. [score:4]
Two miRNAs whose expression was reduced ≥2-fold (miR-982–5p and miR-9b-3p) were also identified (Figs.  4 and 6A). [score:3]
The biological function of miR-982–5p is not known but ectopic expression of pre-miR-982 in the wing pouch using the scalloped-GAL4 driver has been shown to produce phenotypes consistent with a connection to Notch signaling. [score:3]
However, our data shows that pre-miR-982 remains unchanged following Dis3 knockdown. [score:2]
This suggests that Dis3 is involved in the processing of the mature miR-982–5p from the pre-miR-982 precursor. [score:1]
[29] An alternative scenario is that pre-miR-982 binds to particular RNA binding proteins (e. g. Loquacious (PACT in mammals) [42,43]) to enable trimming of the precursor by Dis3. [score:1]
Of all the miRNAs detected by RNA-seq in Dis3 depleted wing imaginal discs, only one, miR-982–5p, showed a decrease in level (-4.4-fold by qRT-PCR) while its precursor remained unchanged. [score:1]
The reasons for this may be twofold; firstly in the absence of Dis3, pre-miR-982 may be signaled for degradation by another 3′-5′ exoribonuclease, for example, by addition of uridyl residues to the 3′ end and degradation by Dis3L2 in a similar manner to that observed during let-7 biogenesis in mouse and human cells. [score:1]
If Dis3 is involved in the processing of pre-miR-982 during its biogenesis then in the absence of Dis3 one may expect the level of precursor to increase. [score:1]
miR-982–5p −2.2/−4.4 Dm Unknown None identified No  Hs – Homo sapiens, Sp – Strongylocentrotus purpuratus, Dm – Drosophila melanogaster, Ce – Caenorhabditis elegans. [score:1]
[40,41] Our data also suggest that Dis3 can specifically affect processing of miR-982–5p from its precursors. [score:1]
[16,18] Alternatively Dis3 could function in the final trimming of miR-982–5p similar to the activity observed for Nibbler. [score:1]
[39,44] In this case the pre-miR-982 would be processed as normal by Dicer and therefore no change in pre-miR-982 levels would be expected. [score:1]
Similarly, miR-982–5p also has a uridine encoded at the next nucleotide after the 3′ end of the mature miRNA in the DNA sequence. [score:1]
** miR-982 is located in close proximity to miR-984, miR-303, miR-983–1 and miR-983–2. [score:1]
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2
[+] score: 22
The cluster of miR-982/2582/303/983/984 appears to be a good example of de novo origin (see below) with point mutations improving miRNA processing step by step (Fig. 2B and Fig. S2 and S3). [score:2]
The miR-982 cluster was examined further by the sliding window analysis of Fay and Wu's H (θ [H]), an estimator of nucleotide diversity sensitive to positive selection [35], [36]. [score:1]
For example, the secondary structure of miR-982 in D. erecta can only form a poor hairpin (−18.20 kcal/mol). [score:1]
As shown in Table 2, all the miRNA genes from the miR-982, miR-303 and miR-983 families have a significantly higher D/P ratio than the flanking regions in both D. melanogaster and D. simulans (p<0.05), suggesting positive selection. [score:1]
Indeed, the burst of adaptive new miRNAs is observable in D. simulans as well as the common ancestor of D. melanogaster and D. simulans, as is evident in the miR-982 cluster (Fig. 2A ). [score:1]
RT-PCR was conducted using total RNA extracted from testes of D. yakuba (for miR-2582/303) or D. erecta (for miR-982/983). [score:1]
The phylogenetic tree of each family of miR-982, miR-2582, miR-303, and miR-983 was reconstructed using the maximum likelihood method as implemented in MEGA 5.0 [69]. [score:1]
Genealogy of miR-982s in Drosophila speciesThe genomic coordinates and precursor sequences of dme-miR-982/303/983-1/983-2/984 and dsi-miR-982c/2582b/982b/2582a/982a/303/983 were retrieved from miRBase (Release v19). [score:1]
1004096.g002 Figure 2(A) This cluster comprises members of five miRNA families (miR-982, miR-2582, miR-303, miR-983 and miR-984), indicated by red, yellow, green, blue and purple boxes, respectively. [score:1]
Orange blocks denote dme-miR-982, dme-miR-303, dme-miR-983-1, dme-miR-983-2 and dme-miR-984 in D. melanogaster, and dsi-miR-982c, dsi-miR-2582b, dsi-miR-982b, dsi-miR-2582a, dsi-miR-982a, dsi-miR-303 and dsi-miR-983 in D. simulans. [score:1]
The ten miRNAs emerged between 4 and 30 Myrs ago are miR-972, miR-978 and miR-2499 from the miR-972s cluster, miR-303, miR-982 and miR-983-1 from the miR-982s cluster, miR-992 and miR-2498 from the miR-310s cluster, miR-1001 and miR-2494. [score:1]
Meanwhile, seed shifting and arm switching occurred in the miR-982/2582/303/983 families (Fig. 2B ). [score:1]
The miR-982s is X-linked, comprising five distinct miRNA families: miR-982, -2582, -303, -983 and -984. [score:1]
The miR-982 cluster consists only of members emerging in the last 30 Myrs, whereas both miR-310s and miR-972s are mixtures of old and new miRNAs (Table S3). [score:1]
a The consensus sequence of dsi-miR-982a/b/c is used as the outgroup of dme-miR-982. [score:1]
The signature is stronger in D. simulans for miR-982 than in D. melanogaster (Fig. S4B and S4C). [score:1]
There were four such candidates: dya-miR-2582-anc, dya-miR-303-anc, der-miR-982-anc, and der-miR-983-anc, where “anc” indicates ancestor. [score:1]
For miR-2582 and miR-303, homologous sequences in D. yakuba were used; for miR-982 and miR-983, homologous sequences in D. erecta were used. [score:1]
miR-2582 and miR-982 were expanded by whole-gene (Fig. 2A, Duplication 1, 2 and 3) or segment duplication (Duplication 4) in D. melanogaster and D. simulans, followed by gene conversion in D. sechellia (Fig. 2A ). [score:1]
b dme-miR-982 is used as the outgroup of dsi-miR-982a/b/c. [score:1]
The genomic coordinates and precursor sequences of dme-miR-982/303/983-1/983-2/984 and dsi-miR-982c/2582b/982b/2582a/982a/303/983 were retrieved from miRBase (Release v19). [score:1]
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3
[+] score: 4
Other miRNAs from this paper: hsa-mir-15a, mmu-mir-15a, dme-mir-252
Another miRNA, miR-982-5p, decreases in expression in Dis3 knock-down discs, suggesting Dis3 may be involved in processing the precursor miRNA into its mature form [80]. [score:4]
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4
[+] score: 1
In addition, SNPs in one long non-coding RNA, CR44190, and downstream of mir-982 are enriched in good performing females. [score:1]
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