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6 publications mentioning dme-mir-986

Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-986. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 46
Differentially expressed miRNAs were identified at the threshold [FDR < 0.05 and log [2](fold change) ≥|1.0|] of 91-C/ 91-R. [b] Fold change was calculated as 91-C/ 91-R. The RT-qPCR validation of the predicted significant quantitative differences in miRNA levels among eight known and four novel miRNAs were amplified showing that the expression levels of miR-986-5p were highly up-regulated in 91-R, whereas miR-286-3p, miR-4919-3p, miR-311-3p, miR-312-3p, and miR-313-3p were significantly down-regulated in 91-R (Fig 1). [score:9]
Differentially expressed miRNAs were identified at the threshold [FDR < 0.05 and log [2](fold change) ≥|1.0|] of 91-C/ 91-R. [b] Fold change was calculated as 91-C/ 91-R. The RT-qPCR validation of the predicted significant quantitative differences in miRNA levels among eight known and four novel miRNAs were amplified showing that the expression levels of miR-986-5p were highly up-regulated in 91-R, whereas miR-286-3p, miR-4919-3p, miR-311-3p, miR-312-3p, and miR-313-3p were significantly down-regulated in 91-R (Fig 1). [score:9]
Specifically, nine miRNAs were significantly down-regulated, and only one (miR-986-5p) was significantly up-regulated in 91-R (25.3-fold) when compared with the susceptible control 91-C (Table 3). [score:6]
Therefore, the miR-986 may be involved in the posttranslational regulation of genes that alleviate oxidative stress induced by DDT insecticide exposures. [score:4]
In contrast, both Cyp6g1 and Cyp6g2 were significantly up-regulated in 91-R, suggesting miR-986-5p may have a transcript stabilizing or enhancing effect, as was demonstrated previously [95, 96]. [score:4]
The function of the most highly up-regulated miRNA in 91-R strain, miR-986-5p, is currently unknown, but levels in the hemolymph of adult virgin males are known to significantly decrease over time [90]. [score:4]
The putative targeted transcripts of miR-986-5p include Cyp6g1 and Cyp6g2. [score:3]
Our transcript target site predictions suggest that miR-986-5p could interact with transcripts of cytochrome P450s, GSTs, esterases, and superoxide dismutases (SODs). [score:3]
Additionally, 4 out of 10 miRNAs predicted to be differentially expressed between 91-R and 91-C (miR-986-5p, miR-995-3p, miR-312-3p, miR-2a-3p) were also predicted to interact with and impact the transcript level of multidrug resistance -associated protein B7 (ABC-B7). [score:3]
Interestingly, the miR-986 precursor is located on the second chromosome within the third intron of the Cyp4e2 gene, which is involved in the metabolism of endogenous and exogenous compounds [91]. [score:1]
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2
[+] score: 8
For example, miR-996-3p and miR-986-5p are expressed at similar levels, yet the former represses its target by over 60% while the latter induces less than 25% repression. [score:5]
We do however note two cases where the minor microRNA* product represses the target more effectively than the dominant arm (bantam and miR-986; compare open and filled points in Figure 1B), despite lower concentration in the cell. [score:3]
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3
[+] score: 7
Figure S1: Total RNA quality control analysis, Figure S2: The insert length distribution of small RNAs, Figure S3: SNP on dme-miR-986-5p, Figure S4: Analysis of 0-2 and 6-8 hour old embryonic RNA-Seq data, Figure S5: Correlation between miRNA and their targets [67], Table S1: The percentage of hairpin and mature miRNA mapped reads in all reads mapped to miRNAs, Table S2: miRNA cluster expression analysis, Table S3: Alignment of the reads to known RNAs. [score:5]
The PCR-amplification and sequencing of dme-miR-986 from P2 strain embryos and S2 cells showed that this particular difference in the sequence stems from a single nucleotide polymorphism (SNP) not an editing process. [score:1]
Based on this approach, we identified one candidate editing event (dme-miR-986, C→T at 11th position) (Figure S3). [score:1]
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4
[+] score: 5
Other miRNAs from this paper: dme-mir-34, dme-bantam, dme-mir-971, dme-mir-1012
This allows us to speculate that the editing may play a major role in what mRNAs are targeted, specifically in the case of mir-986. [score:3]
The functionality of mir-986 is still being elucidated, but the editing is significant due to the fact the site is 1-2 nucleotides away from the seed sequence and the event is conserved through multiple libraries. [score:1]
We found some 30 microRNAs that pass the filter (Table 2), with mir-986 and mir-971 being amongst the microRNAs with the highest editing level. [score:1]
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5
[+] score: 3
These included miR-SPs targeting bantam, miR-1, the K-box family (miR-2b, miR-2c and miR-13b displayed strong phenotypes; miR-2a and miR-13a were flight impaired but fell below our stringent cutoff; ), miR-7, the miR-31 family, miR-34, miR-190, miR-957, miR-986, miR-987 and miR-1001. [score:3]
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6
[+] score: 1
There can be two sub-categories delineated; miRNAs that do not change normally under stress, but do in dystrophic mutants (miR-92a and miR-34) and miRNAs that change as a normal response, but do not in Dys and Dg mutants (miR-956, miR-252, miR-970, miR-137, miR-986, miR-193, miR-1017, miR-962, miR-315, miR-1013, miR-980, miR-975, miR-190, miR-iab-4as-5p, miR-1003 and miR-313). [score:1]
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