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3 publications mentioning cin-let-7a-2

Open access articles that are associated with the species Ciona intestinalis and mention the gene name let-7a-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 17
Therefore, we were not surprised to find the let-7 homolog expressed in the adult tissue of C. intestinalis. [score:3]
For example, let-7 is highly expressed in adult tissue of C. elegans, D. melanogaster, and vertebrates [23]. [score:3]
Our method predicted let-7, miR-7, and miR-126, as well as 11 other conserved miRNA families. [score:1]
Their published prediction of 41 miRNA precursor sequences overlapped with ours by just one microRNA, let-7. In a subsequent study [37], a clustering approach was used with the Ciona non-coding RNA candidate sequences. [score:1]
When hybridizing against the probes for the miR-72 and let-7 Ciona homologs, the C. intestinalis lanes showed strong positive signals. [score:1]
Figures 2 and 3 show the mfold program's text and graphical output for the putative miR-72 and let-7 for Ciona and C. elegans. [score:1]
For the predicted let-7 and miR-72 Ciona miRNA homologs, oligonucleotides were designed as probes for both the top and bottom strands of the hairpin structure. [score:1]
In both the let-7 and miR-72 homologs, no hybridization to the anti-sense strand occurred. [score:1]
Anti-sense (AS) probes were tested for let-7 and miR-72. [score:1]
The C. elegans lanes showed a weak response to the Ciona miR-72 homolog probe, and no response to the Ciona let-7 homolog probe. [score:1]
Similarly, since the Ciona let-7 sequence contained 2 alignment gaps in the middle section the C. elegans sequence, we did not expect to see hybridization. [score:1]
To validate the strand polarity of the predicted mature miRNAs, we performed the Northern blot analysis with sense and anti-sense probes for the top and bottom strands of the let-7 and miR-72 C. intestinalis homolog predictions. [score:1]
By this method, the authors predicted 58 miRNAs, of which only let-7, miR-7, miR-124, and miR-126 coincided with known miRNAs. [score:1]
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2
[+] score: 7
The low adult expression rate likely results from the unequal contribution of different tissue types in the adult body; nevertheless, some miRs are most highly expressed at the adult stage, for example, the let-7 family members, which regulate developmental timing in a variety of animals [20]. [score:7]
[1 to 20 of 1 sentences]
3
[+] score: 4
Among them are tRNAs and U3 snRNA, and an miRNA cluster, Figure 5, which contains the known miRNAs mir-124-a/b and let-7 as well as candidates for mir-126 and mir-7. Clusters 1384 in Figure 6 and 249 in Figure 7 are good candidates for novel ncRNA classes. [score:1]
A more interesting example is a cluster, Figure 5, that contains two paralogs of mir-124 and one copy of let-7 microRNAs that were previously described in computational screens of C. intestinalis [35, 36], as well as good candidates for mir-126 and mir-7. The other members of the cluster have no sequence similarity with known microRNA families compiled in miRBase release 9.0 (blast E ≤ 0.001). [score:1]
Figure 5Cluster Containing Known and Predicted C. intestinalis microRNAsThe two known mir-124 paralogs are members of subcluster 127, whereas the known let-7 is found in subcluster 139. [score:1]
The two known mir-124 paralogs are members of subcluster 127, whereas the known let-7 is found in subcluster 139. [score:1]
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