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1 publications mentioning mml-mir-34c

Open access articles that are associated with the species Macaca mulatta and mention the gene name mir-34c. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 56
The unions of targets predicted by the 9 algorithms at FDR<10% cutoff were used as experimental verified miRNA targets, except for miR-34c-5p, which target FDR was taken at 15% due to a weaker inhibition effect, observed in our transfection experiment (Table S14). [score:9]
To assess whether miRNA with expression divergence on the human lineage might be associated with human cognitive functions, we investigated the expression of genes targeted by five miRNA showing human-specific expression, according to multiple methodologies: miR-184, miR-487a, miR-383, miR-34c-5p and miR-299-3p (Figure 2). [score:7]
Functionally, miR-34c-5p was previously shown to be down-regulated in cancer and Parkinson disease [41]– [44]. [score:6]
Table S16 This table contains GO terms and KEGG pathways enriched in experimental verified targets of miRNA showing human-specific expression, hsa-miR-34c-5p. [score:5]
Putative functions of miR-34c-5p targets were determined using CORNA [65], using experimentally verified target genes of miR-34c-5p as predicted by the 9 aforementioned algorithms, at FDR = 15%. [score:5]
These findings indicate that changes in miR-34c-5p expression on the human evolutionary linage might have resulted in gene expression changes affecting cognitive functions. [score:5]
Thus, although indirectly, these results indicate that the change in miR-34c-5p with human-specific expression might have taken place after the separation of the human and the Neanderthal evolutionary lineages. [score:4]
Requiring significant support by at least two out of three methodologies (sequencing, microarrays and Q-PCR), expression changes in five miRNA (miR-184, miR-299-3p, miR-487a, miR-383 and miR-34c-5p) could be assigned to the human evolutionary lineage and two (miR-375 and miR-154*) to the chimpanzee evolutionary lineage (Figure 2). [score:3]
We indeed found a significant excess of human derived SNPs, indicating the presence of positive selection on the human evolution linage after the human-Neanderthal split, in the upstream regions of one out of five miRNA with human-specific gene expression: miR-34c-5p (Fisher's exact test, Bonferroni corrected p<0.05, ). [score:3]
Signature of positive selection found in the enhancer region of the miRNA, miR-34c-5p, further indicates that this change might have had adaptive significance. [score:1]
On the DNA sequence level, these miRNA tend to be conserved: miR-184 mature miRNA sequence is evolutionarily conserved from insects to humans, with only one nucleotide different at 3′end of mature sequence, while miR-383 and miR-34c-5p are classified as broadly conserved and miR-299-3p - as conserved among animal species [25], [31]. [score:1]
Excess of human derived SNPs in the upstream region of hsa-miR-34c. [score:1]
The plot shows 150kb region upstream of human miR-34c. [score:1]
1002327.g007 Figure 7The plot shows 150kb region upstream of human miR-34c. [score:1]
Notably, for miR-34c-5p signature of positive selection was located in the putative enhancer region approximately 100kb upstream of the miRNA gene (Figure 7). [score:1]
Four windows in an upstream region of miR-34c-5p were significant at Bonferroni corrected p<0.05. [score:1]
We further characterized possible functions of miR-34c-5p in the human brain, based on target genes experimentally verified in cell lines. [score:1]
We chose three types of miRNA differences: (1) consistent by both methodologies: miR-383 and miR-34c-5p; (2) significant according to sequencing, but unconfirmed in the microarray experiment: miR-143 and miR-499; (3) significant according to sequencing, but not detected or masked on the microarrays: miR-184 and miR-299-3p. [score:1]
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